497559 preconverted PubChem assays currently available for download.
AID column links lead to PubChem documentation, links on the right are for direct data file download.
This page contains data for AIDs 1 to 10000.
AID SID count Description KNIME Table CACTVS Table
1 42490 Growth inhibition of the NCI-H23 human Non-Small Cell Lung tumor cell line is measured as a screen for anti-cancer activity. Cells are grown in 96 well plates and exposed to the test compound for 48 hours. Compounds are tested at 5 different concentrations and three endpoints are estimated from this dose response curve: GI50, concentration required for 50% inhibition of growth, TGI, the concentration requires for complete inhibition of growth, and LC50, the concentration required for 50% reductio... aid1.table aid1.tbin
3 39148 Growth inhibition of the NCI-H226 human Non-Small Cell Lung tumor cell line is measured as a screen for anti-cancer activity. Cells are grown in 96 well plates and exposed to the test compound for 48 hours. Compounds are tested at 5 different concentrations and three endpoints are estimated from this dose response curve: GI50, concentration required for 50% inhibition of growth, TGI, the concentration requires for complete inhibition of growth, and LC50, the concentration required for 50% reducti... aid3.table aid3.tbin
5 41160 Growth inhibition of the NCI-H322M human Non-Small Cell Lung tumor cell line is measured as a screen for anti-cancer activity. Cells are grown in 96 well plates and exposed to the test compound for 48 hours. Compounds are tested at 5 different concentrations and three endpoints are estimated from this dose response curve: GI50, concentration required for 50% inhibition of growth, TGI, the concentration requires for complete inhibition of growth, and LC50, the concentration required for 50% reduct... aid5.table aid5.tbin
7 41166 Growth inhibition of the NCI-H460 human Non-Small Cell Lung tumor cell line is measured as a screen for anti-cancer activity. Cells are grown in 96 well plates and exposed to the test compound for 48 hours. Compounds are tested at 5 different concentrations and three endpoints are estimated from this dose response curve: GI50, concentration required for 50% inhibition of growth, TGI, the concentration requires for complete inhibition of growth, and LC50, the concentration required for 50% reducti... aid7.table aid7.tbin
9 41173 Growth inhibition of the HOP-62 human Non-Small Cell Lung tumor cell line is measured as a screen for anti-cancer activity. Cells are grown in 96 well plates and exposed to the test compound for 48 hours. Compounds are tested at 5 different concentrations and three endpoints are estimated from this dose response curve: GI50, concentration required for 50% inhibition of growth, TGI, the concentration requires for complete inhibition of growth, and LC50, the concentration required for 50% reduction... aid9.table aid9.tbin
11 11811 Growth inhibition of the HOP-18 human Non-Small Cell Lung tumor cell line is measured as a screen for anti-cancer activity. Cells are grown in 96 well plates and exposed to the test compound for 48 hours. Compounds are tested at 5 different concentrations and three endpoints are estimated from this dose response curve: GI50, concentration required for 50% inhibition of growth, TGI, the concentration requires for complete inhibition of growth, and LC50, the concentration required for 50% reduction... aid11.table aid11.tbin
13 37537 Growth inhibition of the HOP-92 human Non-Small Cell Lung tumor cell line is measured as a screen for anti-cancer activity. Cells are grown in 96 well plates and exposed to the test compound for 48 hours. Compounds are tested at 5 different concentrations and three endpoints are estimated from this dose response curve: GI50, concentration required for 50% inhibition of growth, TGI, the concentration requires for complete inhibition of growth, and LC50, the concentration required for 50% reduction... aid13.table aid13.tbin
15 38588 Growth inhibition of the NCI-H522 human Non-Small Cell Lung tumor cell line is measured as a screen for anti-cancer activity. Cells are grown in 96 well plates and exposed to the test compound for 48 hours. Compounds are tested at 5 different concentrations and three endpoints are estimated from this dose response curve: GI50, concentration required for 50% inhibition of growth, TGI, the concentration requires for complete inhibition of growth, and LC50, the concentration required for 50% reducti... aid15.table aid15.tbin
17 14224 Growth inhibition of the LXFL 529 human Non-Small Cell Lung tumor cell line is measured as a screen for anti-cancer activity. Cells are grown in 96 well plates and exposed to the test compound for 48 hours. Compounds are tested at 5 different concentrations and three endpoints are estimated from this dose response curve: GI50, concentration required for 50% inhibition of growth, TGI, the concentration requires for complete inhibition of growth, and LC50, the concentration required for 50% reducti... aid17.table aid17.tbin
19 42765 Growth inhibition of the A549/ATCC human Non-Small Cell Lung tumor cell line is measured as a screen for anti-cancer activity. Cells are grown in 96 well plates and exposed to the test compound for 48 hours. Compounds are tested at 5 different concentrations and three endpoints are estimated from this dose response curve: GI50, concentration required for 50% inhibition of growth, TGI, the concentration requires for complete inhibition of growth, and LC50, the concentration required for 50% reduct... aid19.table aid19.tbin
21 41485 Growth inhibition of the EKVX human Non-Small Cell Lung tumor cell line is measured as a screen for anti-cancer activity. Cells are grown in 96 well plates and exposed to the test compound for 48 hours. Compounds are tested at 5 different concentrations and three endpoints are estimated from this dose response curve: GI50, concentration required for 50% inhibition of growth, TGI, the concentration requires for complete inhibition of growth, and LC50, the concentration required for 50% reduction i... aid21.table aid21.tbin
23 39737 Growth inhibition of the LOX IMVI human Melanoma tumor cell line is measured as a screen for anti-cancer activity. Cells are grown in 96 well plates and exposed to the test compound for 48 hours. Compounds are tested at 5 different concentrations and three endpoints are estimated from this dose response curve: GI50, concentration required for 50% inhibition of growth, TGI, the concentration requires for complete inhibition of growth, and LC50, the concentration required for 50% reduction in cell ... aid23.table aid23.tbin
25 41760 Growth inhibition of the M14 human Melanoma tumor cell line is measured as a screen for anti-cancer activity. Cells are grown in 96 well plates and exposed to the test compound for 48 hours. Compounds are tested at 5 different concentrations and three endpoints are estimated from this dose response curve: GI50, concentration required for 50% inhibition of growth, TGI, the concentration requires for complete inhibition of growth, and LC50, the concentration required for 50% reduction in cell numbe... aid25.table aid25.tbin
27 15457 Growth inhibition of the M19-MEL human Melanoma tumor cell line is measured as a screen for anti-cancer activity. Cells are grown in 96 well plates and exposed to the test compound for 48 hours. Compounds are tested at 5 different concentrations and three endpoints are estimated from this dose response curve: GI50, concentration required for 50% inhibition of growth, TGI, the concentration requires for complete inhibition of growth, and LC50, the concentration required for 50% reduction in cell n... aid27.table aid27.tbin
29 39817 Growth inhibition of the MALME-3M human Melanoma tumor cell line is measured as a screen for anti-cancer activity. Cells are grown in 96 well plates and exposed to the test compound for 48 hours. Compounds are tested at 5 different concentrations and three endpoints are estimated from this dose response curve: GI50, concentration required for 50% inhibition of growth, TGI, the concentration requires for complete inhibition of growth, and LC50, the concentration required for 50% reduction in cell ... aid29.table aid29.tbin
31 41599 Growth inhibition of the UACC-62 human Melanoma tumor cell line is measured as a screen for anti-cancer activity. Cells are grown in 96 well plates and exposed to the test compound for 48 hours. Compounds are tested at 5 different concentrations and three endpoints are estimated from this dose response curve: GI50, concentration required for 50% inhibition of growth, TGI, the concentration requires for complete inhibition of growth, and LC50, the concentration required for 50% reduction in cell n... aid31.table aid31.tbin
33 42187 Growth inhibition of the UACC-257 human Melanoma tumor cell line is measured as a screen for anti-cancer activity. Cells are grown in 96 well plates and exposed to the test compound for 48 hours. Compounds are tested at 5 different concentrations and three endpoints are estimated from this dose response curve: GI50, concentration required for 50% inhibition of growth, TGI, the concentration requires for complete inhibition of growth, and LC50, the concentration required for 50% reduction in cell ... aid33.table aid33.tbin
35 39770 Growth inhibition of the SK-MEL-2 human Melanoma tumor cell line is measured as a screen for anti-cancer activity. Cells are grown in 96 well plates and exposed to the test compound for 48 hours. Compounds are tested at 5 different concentrations and three endpoints are estimated from this dose response curve: GI50, concentration required for 50% inhibition of growth, TGI, the concentration requires for complete inhibition of growth, and LC50, the concentration required for 50% reduction in cell ... aid35.table aid35.tbin
37 41516 Growth inhibition of the SK-MEL-5 human Melanoma tumor cell line is measured as a screen for anti-cancer activity. Cells are grown in 96 well plates and exposed to the test compound for 48 hours. Compounds are tested at 5 different concentrations and three endpoints are estimated from this dose response curve: GI50, concentration required for 50% inhibition of growth, TGI, the concentration requires for complete inhibition of growth, and LC50, the concentration required for 50% reduction in cell ... aid37.table aid37.tbin
39 41903 Growth inhibition of the SK-MEL-28 human Melanoma tumor cell line is measured as a screen for anti-cancer activity. Cells are grown in 96 well plates and exposed to the test compound for 48 hours. Compounds are tested at 5 different concentrations and three endpoints are estimated from this dose response curve: GI50, concentration required for 50% inhibition of growth, TGI, the concentration requires for complete inhibition of growth, and LC50, the concentration required for 50% reduction in cell... aid39.table aid39.tbin
41 28694 Growth inhibition of the PC-3 human Prostate tumor cell line is measured as a screen for anti-cancer activity. Cells are grown in 96 well plates and exposed to the test compound for 48 hours. Compounds are tested at 5 different concentrations and three endpoints are estimated from this dose response curve: GI50, concentration required for 50% inhibition of growth, TGI, the concentration requires for complete inhibition of growth, and LC50, the concentration required for 50% reduction in cell numb... aid41.table aid41.tbin
43 28594 Growth inhibition of the DU-145 human Prostate tumor cell line is measured as a screen for anti-cancer activity. Cells are grown in 96 well plates and exposed to the test compound for 48 hours. Compounds are tested at 5 different concentrations and three endpoints are estimated from this dose response curve: GI50, concentration required for 50% inhibition of growth, TGI, the concentration requires for complete inhibition of growth, and LC50, the concentration required for 50% reduction in cell nu... aid43.table aid43.tbin
45 42060 Growth inhibition of the SF-268 human Central Nervous System tumor cell line is measured as a screen for anti-cancer activity. Cells are grown in 96 well plates and exposed to the test compound for 48 hours. Compounds are tested at 5 different concentrations and three endpoints are estimated from this dose response curve: GI50, concentration required for 50% inhibition of growth, TGI, the concentration requires for complete inhibition of growth, and LC50, the concentration required for 50% reduct... aid45.table aid45.tbin
47 42463 Growth inhibition of the SF-295 human Central Nervous System tumor cell line is measured as a screen for anti-cancer activity. Cells are grown in 96 well plates and exposed to the test compound for 48 hours. Compounds are tested at 5 different concentrations and three endpoints are estimated from this dose response curve: GI50, concentration required for 50% inhibition of growth, TGI, the concentration requires for complete inhibition of growth, and LC50, the concentration required for 50% reduct... aid47.table aid47.tbin
49 39798 Growth inhibition of the SF-539 human Central Nervous System tumor cell line is measured as a screen for anti-cancer activity. Cells are grown in 96 well plates and exposed to the test compound for 48 hours. Compounds are tested at 5 different concentrations and three endpoints are estimated from this dose response curve: GI50, concentration required for 50% inhibition of growth, TGI, the concentration requires for complete inhibition of growth, and LC50, the concentration required for 50% reduct... aid49.table aid49.tbin
51 12682 Growth inhibition of the XF 498 human Central Nervous System tumor cell line is measured as a screen for anti-cancer activity. Cells are grown in 96 well plates and exposed to the test compound for 48 hours. Compounds are tested at 5 different concentrations and three endpoints are estimated from this dose response curve: GI50, concentration required for 50% inhibition of growth, TGI, the concentration requires for complete inhibition of growth, and LC50, the concentration required for 50% reduct... aid51.table aid51.tbin
53 41977 Growth inhibition of the SNB-19 human Central Nervous System tumor cell line is measured as a screen for anti-cancer activity. Cells are grown in 96 well plates and exposed to the test compound for 48 hours. Compounds are tested at 5 different concentrations and three endpoints are estimated from this dose response curve: GI50, concentration required for 50% inhibition of growth, TGI, the concentration requires for complete inhibition of growth, and LC50, the concentration required for 50% reduct... aid53.table aid53.tbin
55 39710 Growth inhibition of the SNB-75 human Central Nervous System tumor cell line is measured as a screen for anti-cancer activity. Cells are grown in 96 well plates and exposed to the test compound for 48 hours. Compounds are tested at 5 different concentrations and three endpoints are estimated from this dose response curve: GI50, concentration required for 50% inhibition of growth, TGI, the concentration requires for complete inhibition of growth, and LC50, the concentration required for 50% reduct... aid55.table aid55.tbin
57 14295 Growth inhibition of the SNB-78 human Central Nervous System tumor cell line is measured as a screen for anti-cancer activity. Cells are grown in 96 well plates and exposed to the test compound for 48 hours. Compounds are tested at 5 different concentrations and three endpoints are estimated from this dose response curve: GI50, concentration required for 50% inhibition of growth, TGI, the concentration requires for complete inhibition of growth, and LC50, the concentration required for 50% reduct... aid57.table aid57.tbin
59 42473 Growth inhibition of the U251 human Central Nervous System tumor cell line is measured as a screen for anti-cancer activity. Cells are grown in 96 well plates and exposed to the test compound for 48 hours. Compounds are tested at 5 different concentrations and three endpoints are estimated from this dose response curve: GI50, concentration required for 50% inhibition of growth, TGI, the concentration requires for complete inhibition of growth, and LC50, the concentration required for 50% reductio... aid59.table aid59.tbin
61 14000 Growth inhibition of the DMS 273 human Small Cell Lung tumor cell line is measured as a screen for anti-cancer activity. Cells are grown in 96 well plates and exposed to the test compound for 48 hours. Compounds are tested at 5 different concentrations and three endpoints are estimated from this dose response curve: GI50, concentration required for 50% inhibition of growth, TGI, the concentration requires for complete inhibition of growth, and LC50, the concentration required for 50% reduction in... aid61.table aid61.tbin
63 15159 Growth inhibition of the DMS 114 human Small Cell Lung tumor cell line is measured as a screen for anti-cancer activity. Cells are grown in 96 well plates and exposed to the test compound for 48 hours. Compounds are tested at 5 different concentrations and three endpoints are estimated from this dose response curve: GI50, concentration required for 50% inhibition of growth, TGI, the concentration requires for complete inhibition of growth, and LC50, the concentration required for 50% reduction in... aid63.table aid63.tbin
65 42378 Growth inhibition of the HT29 human Colon tumor cell line is measured as a screen for anti-cancer activity. Cells are grown in 96 well plates and exposed to the test compound for 48 hours. Compounds are tested at 5 different concentrations and three endpoints are estimated from this dose response curve: GI50, concentration required for 50% inhibition of growth, TGI, the concentration requires for complete inhibition of growth, and LC50, the concentration required for 50% reduction in cell number.... aid65.table aid65.tbin
67 42041 Growth inhibition of the COLO 205 human Colon tumor cell line is measured as a screen for anti-cancer activity. Cells are grown in 96 well plates and exposed to the test compound for 48 hours. Compounds are tested at 5 different concentrations and three endpoints are estimated from this dose response curve: GI50, concentration required for 50% inhibition of growth, TGI, the concentration requires for complete inhibition of growth, and LC50, the concentration required for 50% reduction in cell num... aid67.table aid67.tbin
69 14890 Growth inhibition of the DLD-1 human Colon tumor cell line is measured as a screen for anti-cancer activity. Cells are grown in 96 well plates and exposed to the test compound for 48 hours. Compounds are tested at 5 different concentrations and three endpoints are estimated from this dose response curve: GI50, concentration required for 50% inhibition of growth, TGI, the concentration requires for complete inhibition of growth, and LC50, the concentration required for 50% reduction in cell number... aid69.table aid69.tbin
71 42032 Growth inhibition of the HCT-15 human Colon tumor cell line is measured as a screen for anti-cancer activity. Cells are grown in 96 well plates and exposed to the test compound for 48 hours. Compounds are tested at 5 different concentrations and three endpoints are estimated from this dose response curve: GI50, concentration required for 50% inhibition of growth, TGI, the concentration requires for complete inhibition of growth, and LC50, the concentration required for 50% reduction in cell numbe... aid71.table aid71.tbin
73 42223 Growth inhibition of the KM12 human Colon tumor cell line is measured as a screen for anti-cancer activity. Cells are grown in 96 well plates and exposed to the test compound for 48 hours. Compounds are tested at 5 different concentrations and three endpoints are estimated from this dose response curve: GI50, concentration required for 50% inhibition of growth, TGI, the concentration requires for complete inhibition of growth, and LC50, the concentration required for 50% reduction in cell number.... aid73.table aid73.tbin
75 14587 Growth inhibition of the KM20L2 human Colon tumor cell line is measured as a screen for anti-cancer activity. Cells are grown in 96 well plates and exposed to the test compound for 48 hours. Compounds are tested at 5 different concentrations and three endpoints are estimated from this dose response curve: GI50, concentration required for 50% inhibition of growth, TGI, the concentration requires for complete inhibition of growth, and LC50, the concentration required for 50% reduction in cell numbe... aid75.table aid75.tbin
77 38076 Growth inhibition of the HCC-2998 human Colon tumor cell line is measured as a screen for anti-cancer activity. Cells are grown in 96 well plates and exposed to the test compound for 48 hours. Compounds are tested at 5 different concentrations and three endpoints are estimated from this dose response curve: GI50, concentration required for 50% inhibition of growth, TGI, the concentration requires for complete inhibition of growth, and LC50, the concentration required for 50% reduction in cell num... aid77.table aid77.tbin
79 42130 Growth inhibition of the HCT-116 human Colon tumor cell line is measured as a screen for anti-cancer activity. Cells are grown in 96 well plates and exposed to the test compound for 48 hours. Compounds are tested at 5 different concentrations and three endpoints are estimated from this dose response curve: GI50, concentration required for 50% inhibition of growth, TGI, the concentration requires for complete inhibition of growth, and LC50, the concentration required for 50% reduction in cell numb... aid79.table aid79.tbin
81 42732 Growth inhibition of the SW-620 human Colon tumor cell line is measured as a screen for anti-cancer activity. Cells are grown in 96 well plates and exposed to the test compound for 48 hours. Compounds are tested at 5 different concentrations and three endpoints are estimated from this dose response curve: GI50, concentration required for 50% inhibition of growth, TGI, the concentration requires for complete inhibition of growth, and LC50, the concentration required for 50% reduction in cell numbe... aid81.table aid81.tbin
83 29117 Growth inhibition of the MCF7 human Breast tumor cell line is measured as a screen for anti-cancer activity. Cells are grown in 96 well plates and exposed to the test compound for 48 hours. Compounds are tested at 5 different concentrations and three endpoints are estimated from this dose response curve: GI50, concentration required for 50% inhibition of growth, TGI, the concentration requires for complete inhibition of growth, and LC50, the concentration required for 50% reduction in cell number... aid83.table aid83.tbin
85 28746 Growth inhibition of the MDA-MB-435 human Breast tumor cell line is measured as a screen for anti-cancer activity. Cells are grown in 96 well plates and exposed to the test compound for 48 hours. Compounds are tested at 5 different concentrations and three endpoints are estimated from this dose response curve: GI50, concentration required for 50% inhibition of growth, TGI, the concentration requires for complete inhibition of growth, and LC50, the concentration required for 50% reduction in cell ... aid85.table aid85.tbin
87 28140 Growth inhibition of the MDA-N human Breast tumor cell line is measured as a screen for anti-cancer activity. Cells are grown in 96 well plates and exposed to the test compound for 48 hours. Compounds are tested at 5 different concentrations and three endpoints are estimated from this dose response curve: GI50, concentration required for 50% inhibition of growth, TGI, the concentration requires for complete inhibition of growth, and LC50, the concentration required for 50% reduction in cell numbe... aid87.table aid87.tbin
89 25781 Growth inhibition of the BT-549 human Breast tumor cell line is measured as a screen for anti-cancer activity. Cells are grown in 96 well plates and exposed to the test compound for 48 hours. Compounds are tested at 5 different concentrations and three endpoints are estimated from this dose response curve: GI50, concentration required for 50% inhibition of growth, TGI, the concentration requires for complete inhibition of growth, and LC50, the concentration required for 50% reduction in cell numb... aid89.table aid89.tbin
91 27156 Growth inhibition of the T-47D human Breast tumor cell line is measured as a screen for anti-cancer activity. Cells are grown in 96 well plates and exposed to the test compound for 48 hours. Compounds are tested at 5 different concentrations and three endpoints are estimated from this dose response curve: GI50, concentration required for 50% inhibition of growth, TGI, the concentration requires for complete inhibition of growth, and LC50, the concentration required for 50% reduction in cell numbe... aid91.table aid91.tbin
93 29048 Growth inhibition of the NCI/ADR-RES human Breast tumor cell line is measured as a screen for anti-cancer activity. Cells are grown in 96 well plates and exposed to the test compound for 48 hours. Compounds are tested at 5 different concentrations and three endpoints are estimated from this dose response curve: GI50, concentration required for 50% inhibition of growth, TGI, the concentration requires for complete inhibition of growth, and LC50, the concentration required for 50% reduction in cell... aid93.table aid93.tbin
95 28261 Growth inhibition of the MDA-MB-231/ATCC human Breast tumor cell line is measured as a screen for anti-cancer activity. Cells are grown in 96 well plates and exposed to the test compound for 48 hours. Compounds are tested at 5 different concentrations and three endpoints are estimated from this dose response curve: GI50, concentration required for 50% inhibition of growth, TGI, the concentration requires for complete inhibition of growth, and LC50, the concentration required for 50% reduction in ... aid95.table aid95.tbin
97 27096 Growth inhibition of the HS 578T human Breast tumor cell line is measured as a screen for anti-cancer activity. Cells are grown in 96 well plates and exposed to the test compound for 48 hours. Compounds are tested at 5 different concentrations and three endpoints are estimated from this dose response curve: GI50, concentration required for 50% inhibition of growth, TGI, the concentration requires for complete inhibition of growth, and LC50, the concentration required for 50% reduction in cell num... aid97.table aid97.tbin
99 41254 Growth inhibition of the OVCAR-3 human Ovarian tumor cell line is measured as a screen for anti-cancer activity. Cells are grown in 96 well plates and exposed to the test compound for 48 hours. Compounds are tested at 5 different concentrations and three endpoints are estimated from this dose response curve: GI50, concentration required for 50% inhibition of growth, TGI, the concentration requires for complete inhibition of growth, and LC50, the concentration required for 50% reduction in cell nu... aid99.table aid99.tbin
101 42139 Growth inhibition of the IGROV1 human Ovarian tumor cell line is measured as a screen for anti-cancer activity. Cells are grown in 96 well plates and exposed to the test compound for 48 hours. Compounds are tested at 5 different concentrations and three endpoints are estimated from this dose response curve: GI50, concentration required for 50% inhibition of growth, TGI, the concentration requires for complete inhibition of growth, and LC50, the concentration required for 50% reduction in cell num... aid101.table aid101.tbin
103 40316 Growth inhibition of the SK-OV-3 human Ovarian tumor cell line is measured as a screen for anti-cancer activity. Cells are grown in 96 well plates and exposed to the test compound for 48 hours. Compounds are tested at 5 different concentrations and three endpoints are estimated from this dose response curve: GI50, concentration required for 50% inhibition of growth, TGI, the concentration requires for complete inhibition of growth, and LC50, the concentration required for 50% reduction in cell nu... aid103.table aid103.tbin
105 40464 Growth inhibition of the OVCAR-4 human Ovarian tumor cell line is measured as a screen for anti-cancer activity. Cells are grown in 96 well plates and exposed to the test compound for 48 hours. Compounds are tested at 5 different concentrations and three endpoints are estimated from this dose response curve: GI50, concentration required for 50% inhibition of growth, TGI, the concentration requires for complete inhibition of growth, and LC50, the concentration required for 50% reduction in cell nu... aid105.table aid105.tbin
107 41414 Growth inhibition of the OVCAR-5 human Ovarian tumor cell line is measured as a screen for anti-cancer activity. Cells are grown in 96 well plates and exposed to the test compound for 48 hours. Compounds are tested at 5 different concentrations and three endpoints are estimated from this dose response curve: GI50, concentration required for 50% inhibition of growth, TGI, the concentration requires for complete inhibition of growth, and LC50, the concentration required for 50% reduction in cell nu... aid107.table aid107.tbin
109 42713 Growth inhibition of the OVCAR-8 human Ovarian tumor cell line is measured as a screen for anti-cancer activity. Cells are grown in 96 well plates and exposed to the test compound for 48 hours. Compounds are tested at 5 different concentrations and three endpoints are estimated from this dose response curve: GI50, concentration required for 50% inhibition of growth, TGI, the concentration requires for complete inhibition of growth, and LC50, the concentration required for 50% reduction in cell nu... aid109.table aid109.tbin
111 1056 Growth inhibition of the P388 human Leukemia tumor cell line is measured as a screen for anti-cancer activity. Cells are grown in 96 well plates and exposed to the test compound for 48 hours. Compounds are tested at 5 different concentrations and three endpoints are estimated from this dose response curve: GI50, concentration required for 50% inhibition of growth, TGI, the concentration requires for complete inhibition of growth, and LC50, the concentration required for 50% reduction in cell numb... aid111.table aid111.tbin
113 39467 Growth inhibition of the RPMI-8226 human Leukemia tumor cell line is measured as a screen for anti-cancer activity. Cells are grown in 96 well plates and exposed to the test compound for 48 hours. Compounds are tested at 5 different concentrations and three endpoints are estimated from this dose response curve: GI50, concentration required for 50% inhibition of growth, TGI, the concentration requires for complete inhibition of growth, and LC50, the concentration required for 50% reduction in cell... aid113.table aid113.tbin
115 35247 Growth inhibition of the SR human Leukemia tumor cell line is measured as a screen for anti-cancer activity. Cells are grown in 96 well plates and exposed to the test compound for 48 hours. Compounds are tested at 5 different concentrations and three endpoints are estimated from this dose response curve: GI50, concentration required for 50% inhibition of growth, TGI, the concentration requires for complete inhibition of growth, and LC50, the concentration required for 50% reduction in cell number... aid115.table aid115.tbin
117 1034 Growth inhibition of the P388/ADR human Leukemia tumor cell line is measured as a screen for anti-cancer activity. Cells are grown in 96 well plates and exposed to the test compound for 48 hours. Compounds are tested at 5 different concentrations and three endpoints are estimated from this dose response curve: GI50, concentration required for 50% inhibition of growth, TGI, the concentration requires for complete inhibition of growth, and LC50, the concentration required for 50% reduction in cell ... aid117.table aid117.tbin
119 40800 Growth inhibition of the CCRF-CEM human Leukemia tumor cell line is measured as a screen for anti-cancer activity. Cells are grown in 96 well plates and exposed to the test compound for 48 hours. Compounds are tested at 5 different concentrations and three endpoints are estimated from this dose response curve: GI50, concentration required for 50% inhibition of growth, TGI, the concentration requires for complete inhibition of growth, and LC50, the concentration required for 50% reduction in cell ... aid119.table aid119.tbin
121 41721 Growth inhibition of the K-562 human Leukemia tumor cell line is measured as a screen for anti-cancer activity. Cells are grown in 96 well plates and exposed to the test compound for 48 hours. Compounds are tested at 5 different concentrations and three endpoints are estimated from this dose response curve: GI50, concentration required for 50% inhibition of growth, TGI, the concentration requires for complete inhibition of growth, and LC50, the concentration required for 50% reduction in cell num... aid121.table aid121.tbin
123 42140 Growth inhibition of the MOLT-4 human Leukemia tumor cell line is measured as a screen for anti-cancer activity. Cells are grown in 96 well plates and exposed to the test compound for 48 hours. Compounds are tested at 5 different concentrations and three endpoints are estimated from this dose response curve: GI50, concentration required for 50% inhibition of growth, TGI, the concentration requires for complete inhibition of growth, and LC50, the concentration required for 50% reduction in cell nu... aid123.table aid123.tbin
125 38933 Growth inhibition of the HL-60(TB) human Leukemia tumor cell line is measured as a screen for anti-cancer activity. Cells are grown in 96 well plates and exposed to the test compound for 48 hours. Compounds are tested at 5 different concentrations and three endpoints are estimated from this dose response curve: GI50, concentration required for 50% inhibition of growth, TGI, the concentration requires for complete inhibition of growth, and LC50, the concentration required for 50% reduction in cell... aid125.table aid125.tbin
127 1071 Growth inhibition of the SN12K1 human Renal tumor cell line is measured as a screen for anti-cancer activity. Cells are grown in 96 well plates and exposed to the test compound for 48 hours. Compounds are tested at 5 different concentrations and three endpoints are estimated from this dose response curve: GI50, concentration required for 50% inhibition of growth, TGI, the concentration requires for complete inhibition of growth, and LC50, the concentration required for 50% reduction in cell numbe... aid127.table aid127.tbin
129 36746 Growth inhibition of the A498 human Renal tumor cell line is measured as a screen for anti-cancer activity. Cells are grown in 96 well plates and exposed to the test compound for 48 hours. Compounds are tested at 5 different concentrations and three endpoints are estimated from this dose response curve: GI50, concentration required for 50% inhibition of growth, TGI, the concentration requires for complete inhibition of growth, and LC50, the concentration required for 50% reduction in cell number.... aid129.table aid129.tbin
131 39716 Growth inhibition of the CAKI-1 human Renal tumor cell line is measured as a screen for anti-cancer activity. Cells are grown in 96 well plates and exposed to the test compound for 48 hours. Compounds are tested at 5 different concentrations and three endpoints are estimated from this dose response curve: GI50, concentration required for 50% inhibition of growth, TGI, the concentration requires for complete inhibition of growth, and LC50, the concentration required for 50% reduction in cell numbe... aid131.table aid131.tbin
133 37867 Growth inhibition of the RXF 393 human Renal tumor cell line is measured as a screen for anti-cancer activity. Cells are grown in 96 well plates and exposed to the test compound for 48 hours. Compounds are tested at 5 different concentrations and three endpoints are estimated from this dose response curve: GI50, concentration required for 50% inhibition of growth, TGI, the concentration requires for complete inhibition of growth, and LC50, the concentration required for 50% reduction in cell numb... aid133.table aid133.tbin
135 11520 Growth inhibition of the RXF-631 human Renal tumor cell line is measured as a screen for anti-cancer activity. Cells are grown in 96 well plates and exposed to the test compound for 48 hours. Compounds are tested at 5 different concentrations and three endpoints are estimated from this dose response curve: GI50, concentration required for 50% inhibition of growth, TGI, the concentration requires for complete inhibition of growth, and LC50, the concentration required for 50% reduction in cell numb... aid135.table aid135.tbin
137 41560 Growth inhibition of the 786-0 human Renal tumor cell line is measured as a screen for anti-cancer activity. Cells are grown in 96 well plates and exposed to the test compound for 48 hours. Compounds are tested at 5 different concentrations and three endpoints are estimated from this dose response curve: GI50, concentration required for 50% inhibition of growth, TGI, the concentration requires for complete inhibition of growth, and LC50, the concentration required for 50% reduction in cell number... aid137.table aid137.tbin
139 41829 Growth inhibition of the ACHN human Renal tumor cell line is measured as a screen for anti-cancer activity. Cells are grown in 96 well plates and exposed to the test compound for 48 hours. Compounds are tested at 5 different concentrations and three endpoints are estimated from this dose response curve: GI50, concentration required for 50% inhibition of growth, TGI, the concentration requires for complete inhibition of growth, and LC50, the concentration required for 50% reduction in cell number.... aid139.table aid139.tbin
141 41208 Growth inhibition of the TK-10 human Renal tumor cell line is measured as a screen for anti-cancer activity. Cells are grown in 96 well plates and exposed to the test compound for 48 hours. Compounds are tested at 5 different concentrations and three endpoints are estimated from this dose response curve: GI50, concentration required for 50% inhibition of growth, TGI, the concentration requires for complete inhibition of growth, and LC50, the concentration required for 50% reduction in cell number... aid141.table aid141.tbin
143 41858 Growth inhibition of the UO-31 human Renal tumor cell line is measured as a screen for anti-cancer activity. Cells are grown in 96 well plates and exposed to the test compound for 48 hours. Compounds are tested at 5 different concentrations and three endpoints are estimated from this dose response curve: GI50, concentration required for 50% inhibition of growth, TGI, the concentration requires for complete inhibition of growth, and LC50, the concentration required for 50% reduction in cell number... aid143.table aid143.tbin
145 42177 Growth inhibition of the SN12C human Renal tumor cell line is measured as a screen for anti-cancer activity. Cells are grown in 96 well plates and exposed to the test compound for 48 hours. Compounds are tested at 5 different concentrations and three endpoints are estimated from this dose response curve: GI50, concentration required for 50% inhibition of growth, TGI, the concentration requires for complete inhibition of growth, and LC50, the concentration required for 50% reduction in cell number... aid145.table aid145.tbin
147 1792 Growth inhibition of yeast strains with defined genetic alterations is measured as a screen for potential anti-cancer activity. Selected strains with alterations in DNA damage repair or cell cycle control were exposed to compounds and growth inhibition was measured relative to vehicle treated controls. The strain used in this assay is rad52 aid147.table aid147.tbin
149 1792 Growth inhibition of yeast strains with defined genetic alterations is measured as a screen for potential anti-cancer activity. Selected strains with alterations in DNA damage repair or cell cycle control were exposed to compounds and growth inhibition was measured relative to vehicle treated controls. The strain used in this assay is wt1 aid149.table aid149.tbin
151 1792 Growth inhibition of yeast strains with defined genetic alterations is measured as a screen for potential anti-cancer activity. Selected strains with alterations in DNA damage repair or cell cycle control were exposed to compounds and growth inhibition was measured relative to vehicle treated controls. The strain used in this assay is rad50EPP+ aid151.table aid151.tbin
153 1792 Growth inhibition of yeast strains with defined genetic alterations is measured as a screen for potential anti-cancer activity. Selected strains with alterations in DNA damage repair or cell cycle control were exposed to compounds and growth inhibition was measured relative to vehicle treated controls. The strain used in this assay is mgt1 aid153.table aid153.tbin
155 86152 Growth inhibition of yeast strains with defined genetic alterations is measured as a screen for potential anti-cancer activity. Selected strains with alterations in DNA damage repair or cell cycle control were exposed to compounds and growth inhibition was measured relative to vehicle treated controls. The strain used in this assay is rad50 aid155.table aid155.tbin
157 86152 Growth inhibition of yeast strains with defined genetic alterations is measured as a screen for potential anti-cancer activity. Selected strains with alterations in DNA damage repair or cell cycle control were exposed to compounds and growth inhibition was measured relative to vehicle treated controls. The strain used in this assay is mec2-1 aid157.table aid157.tbin
159 1792 Growth inhibition of yeast strains with defined genetic alterations is measured as a screen for potential anti-cancer activity. Selected strains with alterations in DNA damage repair or cell cycle control were exposed to compounds and growth inhibition was measured relative to vehicle treated controls. The strain used in this assay is rad14 aid159.table aid159.tbin
161 85470 Growth inhibition of yeast strains with defined genetic alterations is measured as a screen for potential anti-cancer activity. Selected strains with alterations in DNA damage repair or cell cycle control were exposed to compounds and growth inhibition was measured relative to vehicle treated controls. The strain used in this assay is sgs1 mgt1 aid161.table aid161.tbin
163 1792 Growth inhibition of yeast strains with defined genetic alterations is measured as a screen for potential anti-cancer activity. Selected strains with alterations in DNA damage repair or cell cycle control were exposed to compounds and growth inhibition was measured relative to vehicle treated controls. The strain used in this assay is CLN2oe aid163.table aid163.tbin
165 85470 Growth inhibition of yeast strains with defined genetic alterations is measured as a screen for potential anti-cancer activity. Selected strains with alterations in DNA damage repair or cell cycle control were exposed to compounds and growth inhibition was measured relative to vehicle treated controls. The strain used in this assay is cln2 rad14 aid165.table aid165.tbin
167 85477 Growth inhibition of yeast strains with defined genetic alterations is measured as a screen for potential anti-cancer activity. Selected strains with alterations in DNA damage repair or cell cycle control were exposed to compounds and growth inhibition was measured relative to vehicle treated controls. The strain used in this assay is bub3 aid167.table aid167.tbin
169 1820 Growth inhibition of yeast strains with defined genetic alterations is measured as a screen for potential anti-cancer activity. Selected strains with alterations in DNA damage repair or cell cycle control were exposed to compounds and growth inhibition was measured relative to vehicle treated controls. The strain used in this assay is wt2 aid169.table aid169.tbin
171 1792 Growth inhibition of yeast strains with defined genetic alterations is measured as a screen for potential anti-cancer activity. Selected strains with alterations in DNA damage repair or cell cycle control were exposed to compounds and growth inhibition was measured relative to vehicle treated controls. The strain used in this assay is mlh1 aid171.table aid171.tbin
173 1792 Growth inhibition of yeast strains with defined genetic alterations is measured as a screen for potential anti-cancer activity. Selected strains with alterations in DNA damage repair or cell cycle control were exposed to compounds and growth inhibition was measured relative to vehicle treated controls. The strain used in this assay is sgs1 aid173.table aid173.tbin
175 86130 Growth inhibition of yeast strains with defined genetic alterations is measured as a screen for potential anti-cancer activity. Selected strains with alterations in DNA damage repair or cell cycle control were exposed to compounds and growth inhibition was measured relative to vehicle treated controls. The strain used in this assay is mlh1 rad18 aid175.table aid175.tbin
177 1792 Growth inhibition of yeast strains with defined genetic alterations is measured as a screen for potential anti-cancer activity. Selected strains with alterations in DNA damage repair or cell cycle control were exposed to compounds and growth inhibition was measured relative to vehicle treated controls. The strain used in this assay is rad18 aid177.table aid177.tbin
179 44150 The ability of compounds to protect human CEM cells from HIV-1 infection is measured as a screen for new compounds capable of inhibiting the HIV virus. Five concentrations of drug were tested on uninfected and infected cells and cell growth was measured using a soluble formazan assay. The dose response curve for the uninfected cells was used to calculate an IC50, the concentration of drug that causes 50% inhibition of growth (a measure of toxicity). The dose respose curve for the infected cells w... aid179.table aid179.tbin
180 1348 The antitumor activity of compounds was measured in mice bearing transplantable tumors. Survival or tumor size were measured and the results are expressed as the measurement made in the treated group (T) divided by the measurement made in the vehicle treated control group (C). The tumor model used in this assay is Adenocarcinoma 755 (subcutaneous) in B6D2F1 (BDF1) mice aid180.table aid180.tbin
182 103 The antitumor activity of compounds was measured in mice bearing transplantable tumors. Survival or tumor size were measured and the results are expressed as the measurement made in the treated group (T) divided by the measurement made in the vehicle treated control group (C). The tumor model used in this assay is P388 Leukemia resistant to Adriamycin; NSC 123127, Developed at Scr 06 and 41 (intraperitoneal) in B6D2F1 (BDF1) mice aid182.table aid182.tbin
184 77 The antitumor activity of compounds was measured in mice bearing transplantable tumors. Survival or tumor size were measured and the results are expressed as the measurement made in the treated group (T) divided by the measurement made in the vehicle treated control group (C). The tumor model used in this assay is ADJ-PC-6 (intraperitoneal) in BALB/cJ mice aid184.table aid184.tbin
186 150 The antitumor activity of compounds was measured in mice bearing transplantable tumors. Survival or tumor size were measured and the results are expressed as the measurement made in the treated group (T) divided by the measurement made in the vehicle treated control group (C). The tumor model used in this assay is Nontumored Animals (Toxicity Test) in B6D2F1 (BDF1) mice aid186.table aid186.tbin
188 244 The antitumor activity of compounds was measured in mice bearing transplantable tumors. Survival or tumor size were measured and the results are expressed as the measurement made in the treated group (T) divided by the measurement made in the vehicle treated control group (C). The tumor model used in this assay is Nontumored Animals (Toxicity Test) in CD2F1 (CDF1) mice aid188.table aid188.tbin
190 261 The antitumor activity of compounds was measured in mice bearing transplantable tumors. Survival or tumor size were measured and the results are expressed as the measurement made in the treated group (T) divided by the measurement made in the vehicle treated control group (C). The tumor model used in this assay is Lymphoma AKR (Transplanted) (intraperitoneal) in AKR/Lw mice aid190.table aid190.tbin
192 6211 The antitumor activity of compounds was measured in mice bearing transplantable tumors. Survival or tumor size were measured and the results are expressed as the measurement made in the treated group (T) divided by the measurement made in the vehicle treated control group (C). The tumor model used in this assay is B16 Melanoma (intraperitoneal) in B6D2F1 (BDF1) mice aid192.table aid192.tbin
194 266 The antitumor activity of compounds was measured in mice bearing transplantable tumors. Survival or tumor size were measured and the results are expressed as the measurement made in the treated group (T) divided by the measurement made in the vehicle treated control group (C). The tumor model used in this assay is B16 Melanoma (subcutaneous) in B6D2F1 (BDF1) mice aid194.table aid194.tbin
196 134 The antitumor activity of compounds was measured in mice bearing transplantable tumors. Survival or tumor size were measured and the results are expressed as the measurement made in the treated group (T) divided by the measurement made in the vehicle treated control group (C). The tumor model used in this assay is B16 Melanoma (intracerebral) in B6D2F1 (BDF1) mice aid196.table aid196.tbin
198 119 The antitumor activity of compounds was measured in mice bearing transplantable tumors. Survival or tumor size were measured and the results are expressed as the measurement made in the treated group (T) divided by the measurement made in the vehicle treated control group (C). The tumor model used in this assay is B16 Melanoma (intraperitoneal) in C57BL/6 mice aid198.table aid198.tbin
200 1957 The antitumor activity of compounds was measured in mice bearing transplantable tumors. Survival or tumor size were measured and the results are expressed as the measurement made in the treated group (T) divided by the measurement made in the vehicle treated control group (C). The tumor model used in this assay is B16 Melanoma (intraperitoneal) in B6C3F1 mice aid200.table aid200.tbin
202 107 The antitumor activity of compounds was measured in mice bearing transplantable tumors. Survival or tumor size were measured and the results are expressed as the measurement made in the treated group (T) divided by the measurement made in the vehicle treated control group (C). The tumor model used in this assay is HT29;CX-1 Human Adenocarcinoma (MER+) (subcutaneous) in NU/NU Swiss (nude) mice aid202.table aid202.tbin
204 101 The antitumor activity of compounds was measured in mice bearing transplantable tumors. Survival or tumor size were measured and the results are expressed as the measurement made in the treated group (T) divided by the measurement made in the vehicle treated control group (C). The tumor model used in this assay is P288 Lymphocytic Leukemia resistant to methotrexate; NSC 740 (intraperitoneal) in B6D2F1 (BDF1) mice aid204.table aid204.tbin
206 964 The antitumor activity of compounds was measured in mice bearing transplantable tumors. Survival or tumor size were measured and the results are expressed as the measurement made in the treated group (T) divided by the measurement made in the vehicle treated control group (C). The tumor model used in this assay is HT29;CX-1 Human Adenocarcinoma (MER+) (intrarenal inoculation) in NU/NU Swiss (nude) mice aid206.table aid206.tbin
208 241 The antitumor activity of compounds was measured in mice bearing transplantable tumors. Survival or tumor size were measured and the results are expressed as the measurement made in the treated group (T) divided by the measurement made in the vehicle treated control group (C). The tumor model used in this assay is HT29;CX-1 Human Adenocarcinoma (MER+) (intrarenal inoculation) in NU/NU BALB/C (nude) mice aid208.table aid208.tbin
210 298 The antitumor activity of compounds was measured in mice bearing transplantable tumors. Survival or tumor size were measured and the results are expressed as the measurement made in the treated group (T) divided by the measurement made in the vehicle treated control group (C). The tumor model used in this assay is Colon 26 Adenocarcinoma (intraperitoneal) in CD2F1 (CDF1) mice aid210.table aid210.tbin
212 1251 The antitumor activity of compounds was measured in mice bearing transplantable tumors. Survival or tumor size were measured and the results are expressed as the measurement made in the treated group (T) divided by the measurement made in the vehicle treated control group (C). The tumor model used in this assay is Colon Carcinoma 38 (subcutaneous) in B6D2F1 (BDF1) mice aid212.table aid212.tbin
214 193 The antitumor activity of compounds was measured in mice bearing transplantable tumors. Survival or tumor size were measured and the results are expressed as the measurement made in the treated group (T) divided by the measurement made in the vehicle treated control group (C). The tumor model used in this assay is Colon Carcinoma 38 (subcutaneous) in B6C3F1 mice aid214.table aid214.tbin
216 161 The antitumor activity of compounds was measured in mice bearing transplantable tumors. Survival or tumor size were measured and the results are expressed as the measurement made in the treated group (T) divided by the measurement made in the vehicle treated control group (C). The tumor model used in this assay is P288 Lymphocytic Leukemia (intraperitoneal) in B6D2F1 (BDF1) mice aid216.table aid216.tbin
218 150 The antitumor activity of compounds was measured in mice bearing transplantable tumors. Survival or tumor size were measured and the results are expressed as the measurement made in the treated group (T) divided by the measurement made in the vehicle treated control group (C). The tumor model used in this assay is Adenocarcinoma 755 (subcutaneous) in C57BL/6 mice aid218.table aid218.tbin
220 1239 The antitumor activity of compounds was measured in mice bearing transplantable tumors. Survival or tumor size were measured and the results are expressed as the measurement made in the treated group (T) divided by the measurement made in the vehicle treated control group (C). The tumor model used in this assay is Mammary Adenocarcinoma CD8F1 (subcutaneous) in CD8F1 aid220.table aid220.tbin
222 167 The antitumor activity of compounds was measured in mice bearing transplantable tumors. Survival or tumor size were measured and the results are expressed as the measurement made in the treated group (T) divided by the measurement made in the vehicle treated control group (C). The tumor model used in this assay is Ependymoblastoma (intracerebral) in C57BL/6 mice aid222.table aid222.tbin
224 134 The antitumor activity of compounds was measured in mice bearing transplantable tumors. Survival or tumor size were measured and the results are expressed as the measurement made in the treated group (T) divided by the measurement made in the vehicle treated control group (C). The tumor model used in this assay is P335 Leukemia (intraperitoneal) in B6D2F1 (BDF1) mice aid224.table aid224.tbin
226 283 The antitumor activity of compounds was measured in mice bearing transplantable tumors. Survival or tumor size were measured and the results are expressed as the measurement made in the treated group (T) divided by the measurement made in the vehicle treated control group (C). The tumor model used in this assay is Ependymoblastoma (intracerebral) in B6C3F1 mice aid226.table aid226.tbin
228 337 The antitumor activity of compounds was measured in mice bearing transplantable tumors. Survival or tumor size were measured and the results are expressed as the measurement made in the treated group (T) divided by the measurement made in the vehicle treated control group (C). The tumor model used in this assay is Friend Virus Leukemia (Solid) (subcutaneous) in B6D2F1 (BDF1) mice aid228.table aid228.tbin
230 115 The antitumor activity of compounds was measured in mice bearing transplantable tumors. Survival or tumor size were measured and the results are expressed as the measurement made in the treated group (T) divided by the measurement made in the vehicle treated control group (C). The tumor model used in this assay is Lymphosarcoma Gardner 6C3HED (intraperitoneal) in C3H/He mice aid230.table aid230.tbin
232 85 The antitumor activity of compounds was measured in mice bearing transplantable tumors. Survival or tumor size were measured and the results are expressed as the measurement made in the treated group (T) divided by the measurement made in the vehicle treated control group (C). The tumor model used in this assay is Lymphosarcoma Gardner 6C3HED (intraperitoneal) in C3AKF1 (CHKRF1) mice aid232.table aid232.tbin
234 146 The antitumor activity of compounds was measured in mice bearing transplantable tumors. Survival or tumor size were measured and the results are expressed as the measurement made in the treated group (T) divided by the measurement made in the vehicle treated control group (C). The tumor model used in this assay is Cystadenocarcinoma, Liver (No. 1) (Hamster) (subcutaneous) in C3AKF1 (CHKRF1) mice aid234.table aid234.tbin
236 106 The antitumor activity of compounds was measured in mice bearing transplantable tumors. Survival or tumor size were measured and the results are expressed as the measurement made in the treated group (T) divided by the measurement made in the vehicle treated control group (C). The tumor model used in this assay is AK4 Lymphoid Leukemia (intraperitoneal) in C3AKF1 (CHKRF1) mice aid236.table aid236.tbin
238 86 The antitumor activity of compounds was measured in mice bearing transplantable tumors. Survival or tumor size were measured and the results are expressed as the measurement made in the treated group (T) divided by the measurement made in the vehicle treated control group (C). The tumor model used in this assay is Leiomyosarcoma (No. 2) (intraperitoneal) in CAF1 mice aid238.table aid238.tbin
240 88 The antitumor activity of compounds was measured in mice bearing transplantable tumors. Survival or tumor size were measured and the results are expressed as the measurement made in the treated group (T) divided by the measurement made in the vehicle treated control group (C). The tumor model used in this assay is Lymphoma 4 (intraperitoneal) in B6D2F1 (BDF1) mice aid240.table aid240.tbin
242 104 The antitumor activity of compounds was measured in mice bearing transplantable tumors. Survival or tumor size were measured and the results are expressed as the measurement made in the treated group (T) divided by the measurement made in the vehicle treated control group (C). The tumor model used in this assay is Lymphoma 8 (intraperitoneal) in B6D2F1 (BDF1) mice aid242.table aid242.tbin
244 65 The antitumor activity of compounds was measured in mice bearing transplantable tumors. Survival or tumor size were measured and the results are expressed as the measurement made in the treated group (T) divided by the measurement made in the vehicle treated control group (C). The tumor model used in this assay is Lymphoma 8 (intraperitoneal) in CD2F1 (CDF1) mice aid244.table aid244.tbin
246 162 The antitumor activity of compounds was measured in mice bearing transplantable tumors. Survival or tumor size were measured and the results are expressed as the measurement made in the treated group (T) divided by the measurement made in the vehicle treated control group (C). The tumor model used in this assay is P1081 Chloroleukemia (intraperitoneal) in B6D2F1 (BDF1) mice aid246.table aid246.tbin
248 58883 The antitumor activity of compounds was measured in mice bearing transplantable tumors. Survival or tumor size were measured and the results are expressed as the measurement made in the treated group (T) divided by the measurement made in the vehicle treated control group (C). The tumor model used in this assay is L1210 Leukemia (intraperitoneal) in B6D2F1 (BDF1) mice aid248.table aid248.tbin
250 395 The antitumor activity of compounds was measured in mice bearing transplantable tumors. Survival or tumor size were measured and the results are expressed as the measurement made in the treated group (T) divided by the measurement made in the vehicle treated control group (C). The tumor model used in this assay is L1210 Leukemia (subcutaneous) in B6D2F1 (BDF1) mice aid250.table aid250.tbin
252 307 The antitumor activity of compounds was measured in mice bearing transplantable tumors. Survival or tumor size were measured and the results are expressed as the measurement made in the treated group (T) divided by the measurement made in the vehicle treated control group (C). The tumor model used in this assay is L1210 Leukemia (intracerebral) in B6D2F1 (BDF1) mice aid252.table aid252.tbin
254 49 The antitumor activity of compounds was measured in mice bearing transplantable tumors. Survival or tumor size were measured and the results are expressed as the measurement made in the treated group (T) divided by the measurement made in the vehicle treated control group (C). The tumor model used in this assay is L1210 Leukemia (intravenous) in B6D2F1 (BDF1) mice aid254.table aid254.tbin
256 21196 The antitumor activity of compounds was measured in mice bearing transplantable tumors. Survival or tumor size were measured and the results are expressed as the measurement made in the treated group (T) divided by the measurement made in the vehicle treated control group (C). The tumor model used in this assay is L1210 Leukemia (intraperitoneal) in CD2F1 (CDF1) mice aid256.table aid256.tbin
258 230 The antitumor activity of compounds was measured in mice bearing transplantable tumors. Survival or tumor size were measured and the results are expressed as the measurement made in the treated group (T) divided by the measurement made in the vehicle treated control group (C). The tumor model used in this assay is L1210 Leukemia (subcutaneous) in CD2F1 (CDF1) mice aid258.table aid258.tbin
260 167 The antitumor activity of compounds was measured in mice bearing transplantable tumors. Survival or tumor size were measured and the results are expressed as the measurement made in the treated group (T) divided by the measurement made in the vehicle treated control group (C). The tumor model used in this assay is L1210 Leukemia (intracerebral) in CD2F1 (CDF1) mice aid260.table aid260.tbin
262 129 The antitumor activity of compounds was measured in mice bearing transplantable tumors. Survival or tumor size were measured and the results are expressed as the measurement made in the treated group (T) divided by the measurement made in the vehicle treated control group (C). The tumor model used in this assay is Human Lung LX-1 Xenograft (subcutaneous) in NU/NU Swiss (nude) mice aid262.table aid262.tbin
264 953 The antitumor activity of compounds was measured in mice bearing transplantable tumors. Survival or tumor size were measured and the results are expressed as the measurement made in the treated group (T) divided by the measurement made in the vehicle treated control group (C). The tumor model used in this assay is Human Lung LX-1 Xenograft (intrarenal inoculation) in NU/NU Swiss (nude) mice aid264.table aid264.tbin
266 250 The antitumor activity of compounds was measured in mice bearing transplantable tumors. Survival or tumor size were measured and the results are expressed as the measurement made in the treated group (T) divided by the measurement made in the vehicle treated control group (C). The tumor model used in this assay is Human Lung LX-1 Xenograft (intrarenal inoculation) in NU/NU BALB/C (nude) mice aid266.table aid266.tbin
268 192 The antitumor activity of compounds was measured in mice bearing transplantable tumors. Survival or tumor size were measured and the results are expressed as the measurement made in the treated group (T) divided by the measurement made in the vehicle treated control group (C). The tumor model used in this assay is P1798 Lymphosarcoma (subcutaneous) in CD2F1 (CDF1) mice aid268.table aid268.tbin
270 1042 The antitumor activity of compounds was measured in mice bearing transplantable tumors. Survival or tumor size were measured and the results are expressed as the measurement made in the treated group (T) divided by the measurement made in the vehicle treated control group (C). The tumor model used in this assay is Lewis Lung Carcinoma (subcutaneous) in B6D2F1 (BDF1) mice aid270.table aid270.tbin
272 381 The antitumor activity of compounds was measured in mice bearing transplantable tumors. Survival or tumor size were measured and the results are expressed as the measurement made in the treated group (T) divided by the measurement made in the vehicle treated control group (C). The tumor model used in this assay is Lewis Lung Carcinoma (intramuscular) in B6D2F1 (BDF1) mice aid272.table aid272.tbin
274 543 The antitumor activity of compounds was measured in mice bearing transplantable tumors. Survival or tumor size were measured and the results are expressed as the measurement made in the treated group (T) divided by the measurement made in the vehicle treated control group (C). The tumor model used in this assay is Lewis Lung Carcinoma (intravenous) in B6D2F1 (BDF1) mice aid274.table aid274.tbin
276 612 The antitumor activity of compounds was measured in mice bearing transplantable tumors. Survival or tumor size were measured and the results are expressed as the measurement made in the treated group (T) divided by the measurement made in the vehicle treated control group (C). The tumor model used in this assay is Lewis Lung Carcinoma (intravenous) in B6C3F1 mice aid276.table aid276.tbin
278 224 The antitumor activity of compounds was measured in mice bearing transplantable tumors. Survival or tumor size were measured and the results are expressed as the measurement made in the treated group (T) divided by the measurement made in the vehicle treated control group (C). The tumor model used in this assay is A549 Human Adenocarcinoma of Lung with characteristics of Type II Alveolar Epithelial cells (intrarenal inoculation) in NU/NU BALB/C (nude) mice aid278.table aid278.tbin
280 336 The antitumor activity of compounds was measured in mice bearing transplantable tumors. Survival or tumor size were measured and the results are expressed as the measurement made in the treated group (T) divided by the measurement made in the vehicle treated control group (C). The tumor model used in this assay is Human Amelanotic Melanoma (LOX) (intraperitoneal) in NU/NU BALB/C (nude) mice aid280.table aid280.tbin
282 134 The antitumor activity of compounds was measured in mice bearing transplantable tumors. Survival or tumor size were measured and the results are expressed as the measurement made in the treated group (T) divided by the measurement made in the vehicle treated control group (C). The tumor model used in this assay is L1210 Leukemia resistant to A Terephthalanilide; NSC 38280 (intraperitoneal) in B6D2F1 (BDF1) mice aid282.table aid282.tbin
284 124 The antitumor activity of compounds was measured in mice bearing transplantable tumors. Survival or tumor size were measured and the results are expressed as the measurement made in the treated group (T) divided by the measurement made in the vehicle treated control group (C). The tumor model used in this assay is L1210 Leukemia resistant to Methotrexate; NSC 740 (intraperitoneal) in B6D2F1 (BDF1) mice aid284.table aid284.tbin
286 108 The antitumor activity of compounds was measured in mice bearing transplantable tumors. Survival or tumor size were measured and the results are expressed as the measurement made in the treated group (T) divided by the measurement made in the vehicle treated control group (C). The tumor model used in this assay is Sarcoma M5076 (intraperitoneal) in B6D2F1 (BDF1) mice aid286.table aid286.tbin
288 200 The antitumor activity of compounds was measured in mice bearing transplantable tumors. Survival or tumor size were measured and the results are expressed as the measurement made in the treated group (T) divided by the measurement made in the vehicle treated control group (C). The tumor model used in this assay is Sarcoma M5076 (subcutaneous) in B6D2F1 (BDF1) mice aid288.table aid288.tbin
290 123 The antitumor activity of compounds was measured in mice bearing transplantable tumors. Survival or tumor size were measured and the results are expressed as the measurement made in the treated group (T) divided by the measurement made in the vehicle treated control group (C). The tumor model used in this assay is P1798 Lymphosarcoma (subcutaneous) in unknown mice aid290.table aid290.tbin
292 828 The antitumor activity of compounds was measured in mice bearing transplantable tumors. Survival or tumor size were measured and the results are expressed as the measurement made in the treated group (T) divided by the measurement made in the vehicle treated control group (C). The tumor model used in this assay is Sarcoma M5076 (intraperitoneal) in B6C3F1 mice aid292.table aid292.tbin
294 110 The antitumor activity of compounds was measured in mice bearing transplantable tumors. Survival or tumor size were measured and the results are expressed as the measurement made in the treated group (T) divided by the measurement made in the vehicle treated control group (C). The tumor model used in this assay is Human Mammary Carcinoma MX-1 Xenograft (subcutaneous) in NU/NU Swiss (nude) mice aid294.table aid294.tbin
296 1082 The antitumor activity of compounds was measured in mice bearing transplantable tumors. Survival or tumor size were measured and the results are expressed as the measurement made in the treated group (T) divided by the measurement made in the vehicle treated control group (C). The tumor model used in this assay is Human Mammary Carcinoma MX-1 Xenograft (intrarenal inoculation) in NU/NU Swiss (nude) mice aid296.table aid296.tbin
298 620 The antitumor activity of compounds was measured in mice bearing transplantable tumors. Survival or tumor size were measured and the results are expressed as the measurement made in the treated group (T) divided by the measurement made in the vehicle treated control group (C). The tumor model used in this assay is Human Mammary Carcinoma MX-1 Xenograft (intrarenal inoculation) in NU/NU BALB/C (nude) mice aid298.table aid298.tbin
300 76 The antitumor activity of compounds was measured in mice bearing transplantable tumors. Survival or tumor size were measured and the results are expressed as the measurement made in the treated group (T) divided by the measurement made in the vehicle treated control group (C). The tumor model used in this assay is Madison 109 Lung Carcinoma (intramuscular) in unknown mice aid300.table aid300.tbin
302 78 The antitumor activity of compounds was measured in mice bearing transplantable tumors. Survival or tumor size were measured and the results are expressed as the measurement made in the treated group (T) divided by the measurement made in the vehicle treated control group (C). The tumor model used in this assay is Madison 109 Lung Carcinoma (intramuscular) in BALB/CM mice aid302.table aid302.tbin
304 86 The antitumor activity of compounds was measured in mice bearing transplantable tumors. Survival or tumor size were measured and the results are expressed as the measurement made in the treated group (T) divided by the measurement made in the vehicle treated control group (C). The tumor model used in this assay is Lymphosarcoma Mecca (intraperitoneal) in C3AKF1 (CHKRF1) mice aid304.table aid304.tbin
306 137 The antitumor activity of compounds was measured in mice bearing transplantable tumors. Survival or tumor size were measured and the results are expressed as the measurement made in the treated group (T) divided by the measurement made in the vehicle treated control group (C). The tumor model used in this assay is L1210 Leukemia resistant to Methyl-GAG; NSC 32946 (subcutaneous) in B6D2F1 (BDF1) mice aid306.table aid306.tbin
308 164 The antitumor activity of compounds was measured in mice bearing transplantable tumors. Survival or tumor size were measured and the results are expressed as the measurement made in the treated group (T) divided by the measurement made in the vehicle treated control group (C). The tumor model used in this assay is L1210 Leukemia resistant to 6-MP and 6-Thioguanine; NSC 755, NSC 752 (intraperitoneal) in B6D2F1 (BDF1) mice aid308.table aid308.tbin
310 114 The antitumor activity of compounds was measured in mice bearing transplantable tumors. Survival or tumor size were measured and the results are expressed as the measurement made in the treated group (T) divided by the measurement made in the vehicle treated control group (C). The tumor model used in this assay is Osteogenic Sarcoma HE 10734 (subcutaneous) in C3AKF1 (CHKRF1) mice aid310.table aid310.tbin
312 88 The antitumor activity of compounds was measured in mice bearing transplantable tumors. Survival or tumor size were measured and the results are expressed as the measurement made in the treated group (T) divided by the measurement made in the vehicle treated control group (C). The tumor model used in this assay is C1498 Myeloid Leukemia (intraperitoneal) in B6D2F1 (BDF1) mice aid312.table aid312.tbin
314 127 The antitumor activity of compounds was measured in mice bearing transplantable tumors. Survival or tumor size were measured and the results are expressed as the measurement made in the treated group (T) divided by the measurement made in the vehicle treated control group (C). The tumor model used in this assay is P1534 Leukemia (intraperitoneal) in DBA/2 mice aid314.table aid314.tbin
316 247 The antitumor activity of compounds was measured in mice bearing transplantable tumors. Survival or tumor size were measured and the results are expressed as the measurement made in the treated group (T) divided by the measurement made in the vehicle treated control group (C). The tumor model used in this assay is P1534 Leukemia (intraperitoneal) in CD2F1 (CDF1) mice aid316.table aid316.tbin
318 151 The antitumor activity of compounds was measured in mice bearing transplantable tumors. Survival or tumor size were measured and the results are expressed as the measurement made in the treated group (T) divided by the measurement made in the vehicle treated control group (C). The tumor model used in this assay is P815 Mast Cell Leukemia (Ascitic) (intraperitoneal) in B6D2F1 (BDF1) mice aid318.table aid318.tbin
320 80 The antitumor activity of compounds was measured in mice bearing transplantable tumors. Survival or tumor size were measured and the results are expressed as the measurement made in the treated group (T) divided by the measurement made in the vehicle treated control group (C). The tumor model used in this assay is P329 Reticulum Cell Sarcoma (intraperitoneal) in B6D2F1 (BDF1) mice aid320.table aid320.tbin
322 84 The antitumor activity of compounds was measured in mice bearing transplantable tumors. Survival or tumor size were measured and the results are expressed as the measurement made in the treated group (T) divided by the measurement made in the vehicle treated control group (C). The tumor model used in this assay is P388 Leukemia resistant to Adriamycin; NSC 123127, Developed at Scr 08 (intraperitoneal) in CD2F1 (CDF1) mice aid322.table aid322.tbin
324 67 The antitumor activity of compounds was measured in mice bearing transplantable tumors. Survival or tumor size were measured and the results are expressed as the measurement made in the treated group (T) divided by the measurement made in the vehicle treated control group (C). The tumor model used in this assay is P388 Leukemia resistant to AMSA; NSC 249992 (intraperitoneal) in CD2F1 (CDF1) mice aid324.table aid324.tbin
326 48 The antitumor activity of compounds was measured in mice bearing transplantable tumors. Survival or tumor size were measured and the results are expressed as the measurement made in the treated group (T) divided by the measurement made in the vehicle treated control group (C). The tumor model used in this assay is P388 Leukemia resistant to Dihydroxy Anthracenedione; NSC 299195 (intraperitoneal) in CD2F1 (CDF1) mice aid326.table aid326.tbin
328 12988 The antitumor activity of compounds was measured in mice bearing transplantable tumors. Survival or tumor size were measured and the results are expressed as the measurement made in the treated group (T) divided by the measurement made in the vehicle treated control group (C). The tumor model used in this assay is P388 Leukemia (intraperitoneal) in B6D2F1 (BDF1) mice aid328.table aid328.tbin
330 47318 The antitumor activity of compounds was measured in mice bearing transplantable tumors. Survival or tumor size were measured and the results are expressed as the measurement made in the treated group (T) divided by the measurement made in the vehicle treated control group (C). The tumor model used in this assay is P388 Leukemia (intraperitoneal) in CD2F1 (CDF1) mice aid330.table aid330.tbin
332 136 The antitumor activity of compounds was measured in mice bearing transplantable tumors. Survival or tumor size were measured and the results are expressed as the measurement made in the treated group (T) divided by the measurement made in the vehicle treated control group (C). The tumor model used in this assay is ADJ-PC-20 Plasma Cell (subcutaneous) in unknown mice aid332.table aid332.tbin
334 130 The antitumor activity of compounds was measured in mice bearing transplantable tumors. Survival or tumor size were measured and the results are expressed as the measurement made in the treated group (T) divided by the measurement made in the vehicle treated control group (C). The tumor model used in this assay is P388 Leukemia (intracerebral) in CD2F1 (CDF1) mice aid334.table aid334.tbin
336 152 The antitumor activity of compounds was measured in mice bearing transplantable tumors. Survival or tumor size were measured and the results are expressed as the measurement made in the treated group (T) divided by the measurement made in the vehicle treated control group (C). The tumor model used in this assay is P388 Leukemia resistant to Vincristine; NSC 67574 (intraperitoneal) in B6D2F1 (BDF1) mice aid336.table aid336.tbin
338 110 The antitumor activity of compounds was measured in mice bearing transplantable tumors. Survival or tumor size were measured and the results are expressed as the measurement made in the treated group (T) divided by the measurement made in the vehicle treated control group (C). The tumor model used in this assay is P388 Leukemia resistant to A Terephthalanilide; NSC 38280 (intraperitoneal) in B6D2F1 (BDF1) mice aid338.table aid338.tbin
340 84 The antitumor activity of compounds was measured in mice bearing transplantable tumors. Survival or tumor size were measured and the results are expressed as the measurement made in the treated group (T) divided by the measurement made in the vehicle treated control group (C). The tumor model used in this assay is Reticulum Cell Sarcoma (Kelley Mouse) (intraperitoneal) in CD2F1 (CDF1) mice aid340.table aid340.tbin
342 711 The antitumor activity of compounds was measured in mice bearing transplantable tumors. Survival or tumor size were measured and the results are expressed as the measurement made in the treated group (T) divided by the measurement made in the vehicle treated control group (C). The tumor model used in this assay is Sarcoma 180 (subcutaneous) in Swiss mice aid342.table aid342.tbin
344 98 The antitumor activity of compounds was measured in mice bearing transplantable tumors. Survival or tumor size were measured and the results are expressed as the measurement made in the treated group (T) divided by the measurement made in the vehicle treated control group (C). The tumor model used in this assay is Lieberman Plasma Cell No. 1 (LPC-1) (intraperitoneal) in unknown mice aid344.table aid344.tbin
346 3000 NCGC Assay Overview: HIV-1 nucleocapsid protein (HIV-1 NC) is a small (6.5 kDa) basic zinc-finger protein which participates in packaging of viral genomic RNA. The spacing and metal-chelating residues (3 Cys, 1 His) of the Cys-X2-Cys-X4-His-X4-Cys Zn fingers are conserved among all known retroviruses (J. Med. Chem. 1998, 41, 1371-1381). HIV-1 NC was assayed for its binding to the consensus sequence single-stranded 5'-TGTGTGTG. The assay utilizes fluorescence polarization (FP) where probe TGx4F (... aid346.table aid346.tbin
348 4979 NCGC Assay Overview: Beta-glucocerebrosidase catalyzes the hydrolysis of beta-glucocerebroside to glucose and ceramide. The inherited deficiency of beta-glucocerebrosidase results in Gaucher disease, which is characterized by a wide variety of symptoms including hepatosplenomegaly, anemia, thrombocytopenia, bony lesions and bone marrow infiltration with characteristic storage cells, known as Gaucher cells. There are also forms of the disorder affecting the central nervous system. Patients with ... aid348.table aid348.tbin
351 122 Adult sea urchins were collected from Mediterranean Sea at Cyprus coast and kept in aerated seawater tank. Gametes were obtained by intracoelomic injection of 0.5 M KCl. Eggs were washed with filtered sea water and fertilized by adding drops of a diluted sperm. Embryos were cultured at room temperature under gentle agitation with a motor-driven plastic paddle (60 rpm) in filtered sea water up to the beginning of active feeding (mid-pluteus stage). The embryos were observed with light microscope.... aid351.table aid351.tbin
357 10692 NCGC Assay Overview: Activator protein-1 (AP-1), a transcription factor, plays an important role in tumor genesis by regulating genes involved in cell proliferation, differentiation, apoptosis, and angiogenesis. AP-1 activity is induced by a complex network of signaling pathways that involves extracellular signals, such as growth factors. The AP-1 protein complex formed from c-Fos and c-Jun binding to the AP-1 response element results in transcriptional activation of genes containing such elemen... aid357.table aid357.tbin
360 48125 NCGC Assay Overview: Beta-glucocerebrosidase catalyzes the hydrolysis of beta-glucocerebroside to glucose and ceramide. The inherited deficiency of beta-glucocerebrosidase results in Gaucher disease, which is characterized by a wide variety of symptoms including hepatosplenomegaly, anemia, thrombocytopenia, bony lesions and bone marrow infiltration with characteristic storage cells, known as Gaucher cells. There are also forms of the disorder affecting the central nervous system. Patients with t... aid360.table aid360.tbin
361 51441 NCGC Assay Overview: Pyruvate kinase (partially purified from Bacillus stearothermophilus) was assayed for its ability to generate ATP from ADP using phosphoenolpyruvate (PEP) as a substrate. ATP generation was detected in a coupled reaction by luciferase-mediated luminescence, an ATP-dependent process. Pyruvate kinase substrates, PEP and ADP, were present in the assay at Km and 10-fold below Km respectively. The enzyme was assayed at an intermediate level of activity to screen for inhibitors... aid361.table aid361.tbin
362 4282 University of New Mexico Assay Overview: The formylpeptide receptor (FPR) family of G-protein coupled receptors (GPCR) contributes to the localization and activation of tissue-damaging leukocytes at sites of chronic inflammation. FPR ligands trigger a variety of biologic activities in myeloid cells, including chemokinesis, chemotaxis, cytokine production and superoxide generation. It has been proposed that a primary FPR function is to promote trafficking of phagocytic myeloid cells to sites of... aid362.table aid362.tbin
363 749 This assay contains in vitro affinity data extracted from the literature for compounds tested against human Src protein. aid363.table aid363.tbin
364 3316 The Scripps Research Institute Assay Overview: Compound cytotoxicity is an important parameter to measure when developing potential human therapeutics. To this end, a high-throughput screening (HTS) campaign was designed to measure the metabolic activity of a suspension cell line after challenge & 48 hours of incubation with test compound. For this primary HTS campaign the human T-cell line, Jurkat clone E6.1, was screened against the NIH "starter" set of 3,316 diverse compounds. All compounds w... aid364.table aid364.tbin
365 206 This is a cell-free, enzymatic assay for inhibition of E. coli ribonuclease H (Rnase H) activity. This enzyme differs from that of HIV-1 in lacking a DNA polymerase domain, and by having a substantially higher rate constant. aid365.table aid365.tbin
366 206 This is a cell-free, enzymatic assay for inhibition of human ribonuclease H1 (Rnase H1) activity. This assay has typically been run in dose-response format as a secondary assay to RNAH. aid366.table aid366.tbin
367 206 This is a cell-free, enzymatic assay for inhibition of HIV-2 ribonuclease H (Rnase H) activity. This assay has typically been run in dose-response format as a secondary assay to RNAH. aid367.table aid367.tbin
368 65222 Cdc25B HTS Developed and Run at the University of Pittsburgh Molecular Screening Center (PMLSC) part of the Molecular Library Screening Center Network (MLSCN). XO1 submission MH078959 Cdc25B catalytic domain in vitro assay, Assay Provider Dr. Marni Brisson, Department of Pharmacology at the University of Pittsburgh. Cdc25 is a protein tyrosine phosphatase that plays a pivotal role in the regulation of the cell cycle. Of the three isoforms that exist (Cdc25A, B, and C), Cdc25A and Cdc25B have b... aid368.table aid368.tbin
369 66 This assay contains in vitro affinity data extracted from the literature for compounds tested against Avian Sarcoma Virus Src protein. aid369.table aid369.tbin
370 1 This assay provides a robust method for measuring cytotoxicity in a readily automated 384-well format. Cell viability is determined using the CellTiter Glo reagent (Promega), which gives a luminescent readout of cellular ATP levels. Using this assay we have determined the cytotoxicity of doxorubicin against human pulmonary artery cells (HPAECs), which are a target of the aerosol delivery of therapeutic agents. Doxorubicin was provided spiked in 30 random locations in a 384-plate provided from Di... aid370.table aid370.tbin
371 3317 Compounds that inhibit tumor cell growth may have cytotoxic or cytostatic effects and vary widely in structure and mechanism of action. Cancer chemotherapeutic drugs inhibit tumor cell growth by disrupting cell division or other mechanisms, which often results in apoptotic cell death. The ability of a compound to inhibit the growth of the human non-small cell lung tumor line, A549, is a preliminary indication of anticancer activity for treating patients with lung cancer. In order to screen a... aid371.table aid371.tbin
372 99840 This is a cell-free, enzymatic assay for inhibition of the ribonuclease H (RNase H) activity of the HIV-1 reverse transcriptase (RT) p66/p51 heterodimer. aid372.table aid372.tbin
373 59805 Grant Proposal Number: 1 R03 MH076533-01 The biology of S1P receptor subtypes: Sphingosine 1-phosphate (S1P) influences heart rate [1] [2], coronary artery caliber, endothelial integrity, lung epithelial integrity [3] and lymphocyte recirculation [1] [4]-[6] through five related high affinity G-protein coupled receptors [7]. Inhibition of lymphocyte recirculation by nonselective S1P receptor agonists produces clinical immunosuppression preventing transplant rejection, but is associated with tran... aid373.table aid373.tbin
374 65239 MKP-1 HTS Developed and Run at the University of Pittsburgh Molecular Screening Center (PMLSC) part of the Molecular Library Screening Center Network (MLSCN). XO1 submission MH-76391 In vitro HTS assay for MKP-1, Assay Provider Dr. John S. Lazo, Department of Pharmacology at the University of Pittsburgh. Introduction: brief background and rationale for HTS. The mitogen-activated protein kinases (MAPK) are members of the signaling cascades for diverse extracellular stimuli that regulate fundamen... aid374.table aid374.tbin
375 10011 Southern Research Molecular Libraries Screening Center (SRMLSC) Southern Research Institute (Birmingham, Alabama) NIH Molecular Libraries Screening Centers Network (MLSCN) Award: 1R03MH076412-01 Multi-drug resistant Mycobacterium tuberculosis is becoming an increased health problem, especially in immunocompromised individuals with HIV. This form of TB is more difficult to treat and as a result has a higher mortality rate. Because of this, the discovery of drugs targeting novel pathways such as... aid375.table aid375.tbin
376 1960 Background and Rationale The HERG (human ether-a-go-go-related) K+ channel is a voltage-gated K+ channel involved in repolarizing cardiac cells after a depolarizing stimulus. Mutations in the HERG channel, as well as certain medications, including various antipsychotics and antihistamines, can cause potentially fatal cardiac arrhythmias (e.g. "torsades de pointes") by prolonging the Q-T interval of the cardiac action potential; this effect is a product of blockage of the HERG channel (1). Blocka... aid376.table aid376.tbin
377 779 Rationale Many mammalian cells express membrane proteins that transport large hydrophobic molecules from cells (1). The best studied of these transporters is the multidrug-resistance transporter (MDR, also know as P-glycoprotein), which transports xenobiotic compounds. A wide range of hydrophobic substances are substrates, including steroids, calcium channel blockers, opioids, anticancer drugs, antibiotics, and antipsychotics. MDR is expressed in brain, liver, kidney, the intestines and other ... aid377.table aid377.tbin
378 4 The apparent binding of compounds to Human AASDHPPT has been measured using differential scanning fluorimetry (DSF) technique. In this approach, the unfolding of the protein is monitored via a fluorescent hydrophobicity-sensing dye: SYPRO orange (Invitrogen). The hyperbolic curve of increase in fluorescence intensity versus temperature shows the unfolding process. The point of inflexion of this trace provides the measured parameter known as Tm. A difference in Tm between a control without the co... aid378.table aid378.tbin
379 1 The apparent binding of compounds to Human AK1 has been measured using differential scanning fluorimetry (DSF) technique. In this approach, the unfolding of the protein is monitored via a fluorescent hydrophobicity-sensing dye: SYPRO orange (Invitrogen). The hyperbolic curve of increase in fluorescence intensity versus temperature shows the unfolding process. The point of inflexion of this trace provides the measured parameter known as Tm. A difference in Tm between a control without the compoun... aid379.table aid379.tbin
380 1 The apparent binding of compounds to Human AK3 has been measured using differential scanning fluorimetry (DSF) technique. In this approach, the unfolding of the protein is monitored via a fluorescent hydrophobicity-sensing dye: SYPRO orange (Invitrogen). The hyperbolic curve of increase in fluorescence intensity versus temperature shows the unfolding process. The point of inflexion of this trace provides the measured parameter known as Tm. A difference in Tm between a control without the compoun... aid380.table aid380.tbin
381 12 The apparent binding of compounds to Human AKR1C4 has been measured using differential scanning fluorimetry (DSF) technique. In this approach, the unfolding of the protein is monitored via a fluorescent hydrophobicity-sensing dye: SYPRO orange (Invitrogen). The hyperbolic curve of increase in fluorescence intensity versus temperature shows the unfolding process. The point of inflexion of this trace provides the measured parameter known as Tm. A difference in Tm between a control without the comp... aid381.table aid381.tbin
382 121 The apparent binding of compounds to Human CLK1 has been measured using differential scanning fluorimetry (DSF) technique. In this approach, the unfolding of the protein is monitored via a fluorescent hydrophobicity-sensing dye: SYPRO orange (Invitrogen). The hyperbolic curve of increase in fluorescence intensity versus temperature shows the unfolding process. The point of inflexion of this trace provides the measured parameter known as Tm. A difference in Tm between a control without the compou... aid382.table aid382.tbin
383 55 The apparent binding of compounds to Human CLK3 has been measured using differential scanning fluorimetry (DSF) technique. In this approach, the unfolding of the protein is monitored via a fluorescent hydrophobicity-sensing dye: SYPRO orange (Invitrogen). The hyperbolic curve of increase in fluorescence intensity versus temperature shows the unfolding process. The point of inflexion of this trace provides the measured parameter known as Tm. A difference in Tm between a control without the compou... aid383.table aid383.tbin
384 29 The apparent binding of compounds to Human CSNK1G2 has been measured using differential scanning fluorimetry (DSF) technique. In this approach, the unfolding of the protein is monitored via a fluorescent hydrophobicity-sensing dye: SYPRO orange (Invitrogen). The hyperbolic curve of increase in fluorescence intensity versus temperature shows the unfolding process. The point of inflexion of this trace provides the measured parameter known as Tm. A difference in Tm between a control without the com... aid384.table aid384.tbin
385 17 The apparent binding of compounds to Human CSNK1G3 has been measured using differential scanning fluorimetry (DSF) technique. In this approach, the unfolding of the protein is monitored via a fluorescent hydrophobicity-sensing dye: SYPRO orange (Invitrogen). The hyperbolic curve of increase in fluorescence intensity versus temperature shows the unfolding process. The point of inflexion of this trace provides the measured parameter known as Tm. A difference in Tm between a control without the com... aid385.table aid385.tbin
386 4 The apparent binding of compounds to Human DIRAS has been measured using differential scanning fluorimetry (DSF) technique. In this approach, the unfolding of the protein is monitored via a fluorescent hydrophobicity-sensing dye: SYPRO orange (Invitrogen). The hyperbolic curve of increase in fluorescence intensity versus temperature shows the unfolding process. The point of inflexion of this trace provides the measured parameter known as Tm. A difference in Tm between a control without the compo... aid386.table aid386.tbin
387 2 The apparent binding of compounds to Human FDPS has been measured using differential scanning fluorimetry (DSF) technique. In this approach, the unfolding of the protein is monitored via a fluorescent hydrophobicity-sensing dye: SYPRO orange (Invitrogen). The hyperbolic curve of increase in fluorescence intensity versus temperature shows the unfolding process. The point of inflexion of this trace provides the measured parameter known as Tm. A difference in Tm between a control without the compou... aid387.table aid387.tbin
388 16 The apparent binding of compounds to Human HPGD has been measured using differential scanning fluorimetry (DSF) technique. In this approach, the unfolding of the protein is monitored via a fluorescent hydrophobicity-sensing dye: SYPRO orange (Invitrogen). The hyperbolic curve of increase in fluorescence intensity versus temperature shows the unfolding process. The point of inflexion of this trace provides the measured parameter known as Tm. A difference in Tm between a control without the compou... aid388.table aid388.tbin
389 25 The apparent binding of compounds to Human PAK4 has been measured using differential scanning fluorimetry (DSF) technique. In this approach, the unfolding of the protein is monitored via a fluorescent hydrophobicity-sensing dye: SYPRO orange (Invitrogen). The hyperbolic curve of increase in fluorescence intensity versus temperature shows the unfolding process. The point of inflexion of this trace provides the measured parameter known as Tm. A difference in Tm between a control without the compou... aid389.table aid389.tbin
390 22 The apparent binding of compounds to Human PAK5 has been measured using differential scanning fluorimetry (DSF) technique. In this approach, the unfolding of the protein is monitored via a fluorescent hydrophobicity-sensing dye: SYPRO orange (Invitrogen). The hyperbolic curve of increase in fluorescence intensity versus temperature shows the unfolding process. The point of inflexion of this trace provides the measured parameter known as Tm. A difference in Tm between a control without the compou... aid390.table aid390.tbin
391 25 The apparent binding of compounds to Human PAK6 has been measured using differential scanning fluorimetry (DSF) technique. In this approach, the unfolding of the protein is monitored via a fluorescent hydrophobicity-sensing dye: SYPRO orange (Invitrogen). The hyperbolic curve of increase in fluorescence intensity versus temperature shows the unfolding process. The point of inflexion of this trace provides the measured parameter known as Tm. A difference in Tm between a control without the compou... aid391.table aid391.tbin
392 13 The apparent binding of compounds to Human PECR has been measured using differential scanning fluorimetry (DSF) technique. In this approach, the unfolding of the protein is monitored via a fluorescent hydrophobicity-sensing dye: SYPRO orange (Invitrogen). The hyperbolic curve of increase in fluorescence intensity versus temperature shows the unfolding process. The point of inflexion of this trace provides the measured parameter known as Tm. A difference in Tm between a control without the compou... aid392.table aid392.tbin
393 106 The apparent binding of compounds to Human PIM1 has been measured using differential scanning fluorimetry (DSF) technique. In this approach, the unfolding of the protein is monitored via a fluorescent hydrophobicity-sensing dye: SYPRO orange (Invitrogen). The hyperbolic curve of increase in fluorescence intensity versus temperature shows the unfolding process. The point of inflexion of this trace provides the measured parameter known as Tm. A difference in Tm between a control without the compou... aid393.table aid393.tbin
394 5 The apparent binding of compounds to Human PTPN14 has been measured using differential scanning fluorimetry (DSF) technique. In this approach, the unfolding of the protein is monitored via a fluorescent hydrophobicity-sensing dye: SYPRO orange (Invitrogen). The hyperbolic curve of increase in fluorescence intensity versus temperature shows the unfolding process. The point of inflexion of this trace provides the measured parameter known as Tm. A difference in Tm between a control without the comp... aid394.table aid394.tbin
395 55 The apparent binding of compounds to Human STK16 has been measured using differential scanning fluorimetry (DSF) technique. In this approach, the unfolding of the protein is monitored via a fluorescent hydrophobicity-sensing dye: SYPRO orange (Invitrogen). The hyperbolic curve of increase in fluorescence intensity versus temperature shows the unfolding process. The point of inflexion of this trace provides the measured parameter known as Tm. A difference in Tm between a control without the compo... aid395.table aid395.tbin
396 3 The apparent binding of compounds to Human YWHAB has been measured using differential scanning fluorimetry (DSF) technique. In this approach, the unfolding of the protein is monitored via a fluorescent hydrophobicity-sensing dye: SYPRO orange (Invitrogen). The hyperbolic curve of increase in fluorescence intensity versus temperature shows the unfolding process. The point of inflexion of this trace provides the measured parameter known as Tm. A difference in Tm between a control without the compo... aid396.table aid396.tbin
397 31 The apparent binding of compounds to Human GEM has been measured using differential scanning fluorimetry (DSF) technique. In this approach, the unfolding of the protein is monitored via a fluorescent hydrophobicity-sensing dye: SYPRO orange (Invitrogen). The hyperbolic curve of increase in fluorescence intensity versus temperature shows the unfolding process. The point of inflexion of this trace provides the measured parameter known as Tm. A difference in Tm between a control without the compoun... aid397.table aid397.tbin
398 7 The apparent binding of compounds to Human HSD11B1 has been measured using differential scanning fluorimetry (DSF) technique. In this approach, the unfolding of the protein is monitored via a fluorescent hydrophobicity-sensing dye: SYPRO orange (Invitrogen). The hyperbolic curve of increase in fluorescence intensity versus temperature shows the unfolding process. The point of inflexion of this trace provides the measured parameter known as Tm. A difference in Tm between a control without the com... aid398.table aid398.tbin
399 5 Assay Overview: This assay was developed to determine the cytotoxic effects of small molecule compounds on Jurkat E6-1 cells in a 384 well format. It is slightly modified from the procedure in AID:364. In this protocol, ATP-lite 1 step (Perkin Elmer) is used to determine cell viability via a luminescent readout of cellular ATP levels. aid399.table aid399.tbin
400 36 STEP, a striatal-enriched protein tyrosine phosphatase, is preferentially expressed in neurons of the basal ganglia, hippocampus, cortex and related structures. Alternative splicing produces various STEP family members, and both cytosolic (STEP 46) and membrane-associated (STEP 61) variants exist. STEP and its non-neuronal homologs like He-PTP have been implicated in the regulation of ERK activity. Both splice products STEP 61 and STEP 46 are phosphorylated in a common kinase-interacting domain (... aid400.table aid400.tbin
401 45 PTPN7 (also named leukocyte PTP (LPTP) and hematopoetic PTP (HEPTP)) is a member of the protein tyrosine phosphatase (PTP) family. It is preferentially expressed in a variety of hematopoietic cells, particularly B and T lymphocytes, and is an early response gene in lymphokine stimulated cells. PTPN7 belongs to a subgroup of PTPs with two other members (PTPN5 and PTPRR) which have a non-catalytic N-terminal kinase interaction motif (KIM). These phosphatases interact with and negatively regulate mi... aid401.table aid401.tbin
402 19 PTPN14 (Pez) is a member of the cytoplamic FERM-domain containing protein tyrosine phosphatase (PTP) family which are characterized by a FERM (Band 4.1, ezrin, radixin, moesin homology) domains at their N-termini, and PTP (protein tyrosine phosphatase) domains at their C-termini. PTPN14 was first cloned in a screen for PTPs expressed in normal breast tissue. It is also expressed in varying amounts in other tissues including kidney, skeletal muscle, lung and placenta. In addition, PTPN14 is highly... aid402.table aid402.tbin
403 15 PTPRJ is a member of the R3 family of protein tyrosine phosphatases. It possesses an extracellular region containing fibronectin type III repeats, a single transmembrane region, and a single intracytoplasmic catalytic domain. PTPRJ is expressed in all hematopoietic lineages in particular in granulocytes and monocytes/macrophages. Weaker expression levels have been described for peripheral blood lymphocytes, including CD4 and CD8-positive T-cell subsets, B cells- in particular memory B cells- plat... aid403.table aid403.tbin
404 34 PTPRK belongs together with PTPRM, PTPRT and PTPRU to the R2A/IIb subfamily of receptor protein tyrosine phosphatases. The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPRK possesses an extracellular region, a single transmembrane region, and two tandem catalytic domains. The extracellular region contains a meprin-A5 antigen-PTP mu (MAM) domain, an Ig-like domain and four fibronectin type III-like repeats. The purified extracellular domain of PTPRK f... aid404.table aid404.tbin
405 30 The tyrosine receptor phosphatase PTPRR is composed of an extra cellular region, a single transmembrane region, and a single intracellular catalytic domain which classifies this phosphatase as a receptor class 7 family member. The mouse homologue (PTPR-SL) is predominately expressed in brain and was shown to regulate activity and cellular localization of MAP kinases. A 16 amino acid kinase interaction motif (KIM domain) is critical for binding to MAP kinases. PTPRR was shown to potently reduce ER... aid405.table aid405.tbin
406 13 RNA triphosphatase catalyzes the hydrolysis of the gamma-phosphate of nascent pre-mRNA to form a diphosphate end which is subsequently capped with GMP by RNA guanyltransferase and methylated by (guanine-7) methyltransferase to yield mature m-RNA. These three enzymatic activities are present as separate polypeptides in yeasts. Metazoans including humans contain 2 genes: a separate cap methyltransferase (RNA guanine-7-methyltransferase, RNMT), and a bifunctional capping enzyme (RNGTT) encoded by a ... aid406.table aid406.tbin
410 9198 NCGC Assay Overview: The P450 gene superfamily is involved in metabolism and the clearance of xenobiotics. This assay used human CYP1A2 to measure the demethylation of luciferin 6' methyl ether (Luciferin-ME; Promega-Glo) to luciferin. The luciferin is then measured by luminescence after the addition of a luciferase detection regeant. Luciferin-ME concentration in the assay was equal to its Km for CYP1A2. aid410.table aid410.tbin
411 72359 To aid in the interpretation of high-throughput screening (HTS) results derived from luciferase-based assays, we used quantitative HTS, an approach that defines the concentration-response behavior of each library sample, to profile the ATP-dependent luciferase from Photinus pyralis against more than 72 000 samples. Luciferase (PKLight, Cambrex Corporation) was assayed for its ability to generate light using ATP and luciferin as substrates. The ATP concentration in the assay (10 uM) was within t... aid411.table aid411.tbin
414 5 This assay contains in vitro affinity data extracted from the literature for compounds tested against HIV-1 Protease mutant_L10I,L19Q,K20R,E35D,M36I,S37N,M46I,I50V,I54V,I62V,L63P,A71V,V82A,L90M. The target sequence for wild type is as following: PQVTLWQRPL VTIKIGGQLK EALLDTGADD TVLEEMSLPG RWKPKMIGGI GGFIKVRQYD QILIEICGHK AIGTVLVGPT PVNIIGRNLL TQIGCTLNF(chainA), PQVTLWQRPL VTIKIGGQLK EALLDTGADD TVLEEMSLPG RWKPKMIGGI GGFIKVRQYD QILIEICGHK AIGTVLVGPT PVNIIGRNLL TQIGCTLNF(chainB). aid414.table aid414.tbin
417 1420 This assay contains in vitro affinity data extracted from the literature for compounds tested against HIV-1 Protease. The target sequence for wild type is as following: PQVTLWQRPL VTIKIGGQLK EALLDTGADD TVLEEMSLPG RWKPKMIGGI GGFIKVRQYD QILIEICGHK AIGTVLVGPT PVNIIGRNLL TQIGCTLNF(chainA), PQVTLWQRPL VTIKIGGQLK EALLDTGADD TVLEEMSLPG RWKPKMIGGI GGFIKVRQYD QILIEICGHK AIGTVLVGPT PVNIIGRNLL TQIGCTLNF(chainB). aid417.table aid417.tbin
418 5 This assay contains in vitro affinity data extracted from the literature for compounds tested against HIV-1 Protease mutant_I50V. The target sequence for wild type is as following: PQVTLWQRPL VTIKIGGQLK EALLDTGADD TVLEEMSLPG RWKPKMIGGI GGFIKVRQYD QILIEICGHK AIGTVLVGPT PVNIIGRNLL TQIGCTLNF(chainA), PQVTLWQRPL VTIKIGGQLK EALLDTGADD TVLEEMSLPG RWKPKMIGGI GGFIKVRQYD QILIEICGHK AIGTVLVGPT PVNIIGRNLL TQIGCTLNF(chainB). aid418.table aid418.tbin
419 5 This assay contains in vitro affinity data extracted from the literature for compounds tested against HIV-1 Protease mutant_M46I,L63P,A71V,V82F,I84V. The target sequence for wild type is as following: PQVTLWQRPL VTIKIGGQLK EALLDTGADD TVLEEMSLPG RWKPKMIGGI GGFIKVRQYD QILIEICGHK AIGTVLVGPT PVNIIGRNLL TQIGCTLNF(chainA), PQVTLWQRPL VTIKIGGQLK EALLDTGADD TVLEEMSLPG RWKPKMIGGI GGFIKVRQYD QILIEICGHK AIGTVLVGPT PVNIIGRNLL TQIGCTLNF(chainB). aid419.table aid419.tbin
420 3 This assay contains in vitro affinity data extracted from the literature for compounds tested against HIV-1 Protease mutant_Q7K. The target sequence for wild type is as following: PQVTLWQRPL VTIKIGGQLK EALLDTGADD TVLEEMSLPG RWKPKMIGGI GGFIKVRQYD QILIEICGHK AIGTVLVGPT PVNIIGRNLL TQIGCTLNF(chainA), PQVTLWQRPL VTIKIGGQLK EALLDTGADD TVLEEMSLPG RWKPKMIGGI GGFIKVRQYD QILIEICGHK AIGTVLVGPT PVNIIGRNLL TQIGCTLNF(chainB). aid420.table aid420.tbin
421 1408 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] National Institutes of Environmental Health Sciences [NIEHS] National Toxicology Program [NTP] NCGC Assay Overview: We have developed a 1536-well cell-based assay for quantitative high throughput screening (qHTS) against a number of cell lines to determine in vitro cytotoxicity of small molecules. This particular assay uses the BJ cell line from ATCC which is derived from normal human foreskin fibroblas... aid421.table aid421.tbin
422 163857 NIH Molecular Libraries Screening Centers Network [MLSCN] Emory Chemical Biology Discovery Center in MLSCN MLSCN Grant: 1 X01MH78953-01 The 14-3-3 proteins are the prototype for a novel class of protein modules that can recognize phosphoserine/threonine (pS/T)-containing motifs in a variety of signaling proteins. To date, 14-3-3 proteins have been reported to bind more than 200 client proteins. Through these interactions, 14-3-3 proteins play important roles in a wide range of vital regulatory p... aid422.table aid422.tbin
423 10 Each molecule is identified by a unique six digit ADD number assigned internally by the NINDS' Anticonvulsant Screening Program (ASP). The qualitative screening results are represented as a summary of test data generated for any specific compound. Compounds are tested in the maximal electroshock (MES), subcutaneous Metrazol (scMET), and/or 6Hz models. A limited number of animals are utilized at different doses and time points. For the current reporting purposes if any animal is protected from... aid423.table aid423.tbin
424 5 Each molecule is identified by a unique six digit ADD number assigned internally by the NINDS' Anticonvulsant Screening Program (ASP). A summary of quantitative screening results are provided for specific compounds. This data represents an overview of quantitative data generated in the NINDS Anticonvulsant Screening Program. Compounds are tested in several models (e.g., the maximal electroshock (MES), subcutaneous Metrazol (scMET), 6Hz, kindled rat) using 6-8 animals per dose level. More compl... aid424.table aid424.tbin
425 114459 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Number: XO1 MH076390-01 Assay Provider: Dr. John Lazo, University of Pittsburg MKP-3 (mitogen-activated protein kinase phosphatase-3; EC 3.1.3.48, EC 3.1.3.16), a dual specificity phosphatase negatively regulates ERK1/2 by catalyzing the removal of a phosphoryl group from T... aid425.table aid425.tbin
426 1408 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] National Institutes of Environmental Health Sciences [NIEHS] National Toxicology Program [NTP] NCGC Assay Overview: We have developed a 1536-well cell-based assay for quantitative high throughput screening (qHTS) against a number of cell lines to determine in vitro cytotoxicity of small molecules. This particular assay uses the Jurkat cell line (Clone E6-1) which is derived from the human T cell leukemi... aid426.table aid426.tbin
427 1408 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] National Institutes of Environmental Health Sciences [NIEHS] National Toxicology Program [NTP] NCGC Assay Overview: We have developed a 1536-well cell-based assay for quantitative high throughput screening (qHTS) against a number of cell lines to determine in vitro cytotoxicity of small molecules. This particular assay uses the Hek 293 cell line which is derived from human embryonic kidney cells (transf... aid427.table aid427.tbin
429 63918 Emory Chemistry-Biology Discovery Center Assay Overview: Hsp90 is a chaperon with important roles in maintaining transformation and in elevating the survival and growth potential of cancer cells. Recent evidence suggests additional applications of Hsp90 inhibitors in neurodegenerative diseases, nerve injuries, inflammation and infection. Several natural products that inactivate Hsp90 function have anti-tumor effects in in vitro and in vivo models of cancer. However, due to the role of Hsp90... aid429.table aid429.tbin
430 62662 Sanford-Burnham Center for Chemical Genomics (SBCCG) Sanford-Burnham Medical Research Institute (San Diego, CA) NIH Molecular Libraries Screening Centers Network (MLSCN) One of the most deadly forms of cancer in humans is pancreatic cancer. Typically few individuals survive beyond 12 months after diagnosis. The oncogene KRAS has been suggested to play a role in this disease. Mutations which activate KRAS are almost always found in pancreatic adenocarcinoma. This assay was developed to determin... aid430.table aid430.tbin
431 62661 Sanford-Burnham Center for Chemical Genomics (SBCCG) Sanford-Burnham Medical Research Institute (San Diego, CA) NIH Molecular Libraries Screening Centers Network (MLSCN) One of the most deadly forms of cancer in humans is pancreatic cancer. Typically few individuals survive beyond 12 months after diagnosis. The oncogene KRAS has been suggested to play a role in this disease. Mutations which activate KRAS are almost always found in pancreatic adenocarcinoma. This assay was developed to determine... aid431.table aid431.tbin
432 64394 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Number: XO1 MH077632-01 Assay Provider: Dr. John C. Reed, Sanford-Sanford-Burnham Medical Research Institute Bfl-1, also known as A1 in mice is an anti-apoptotic and NF-kB-inducible member of the Bcl-2 protein family involved in regulation of apoptosis. Due to difficulties ... aid432.table aid432.tbin
433 1408 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] National Institutes of Environmental Health Sciences [NIEHS] National Toxicology Program [NTP] NCGC Assay Overview: We have developed a 1536-well cell-based assay for quantitative high throughput screening (qHTS) against a number of cell lines to determine in vitro cytotoxicity of small molecules. This particular assay uses the HepG2 cell line which is derived from hepatocellular carcinoma. aid433.table aid433.tbin
434 1408 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] National Institutes of Environmental Health Sciences [NIEHS] National Toxicology Program [NTP] NCGC Assay Overview: We have developed a 1536-well cell-based assay for quantitative high throughput screening (qHTS) against a number of cell lines to determine in vitro cytotoxicity of small molecules. This particular assay uses the MRC5 cell line which is derived from normal human lung fibroblasts. aid434.table aid434.tbin
435 1408 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] National Institutes of Environmental Health Sciences [NIEHS] National Toxicology Program [NTP] NCGC Assay Overview: We have developed a 1536-well cell-based assay for quantitative high throughput screening (qHTS) against a number of cell lines to determine in vitro cytotoxicity of small molecules. This particular assay uses the SK-N-SH cell line which is derived from human neuroblastoma. aid435.table aid435.tbin
436 71537 NIH Molecular Libraries Screening Centers Network [MLSCN] Emory Chemical Biology Discovery Center in MLSCN Assay provider: Keith D. Wilkinson, Emory University MLSCN Grant: 1 R03 MH076382-01 Assay Overview: BAP1 (BRCA1 associated protein 1) is a member of the Ubiquitin carboxy-terminal hydrolase (UCH) family of deubiquitinating enzymes(DUB). These proteases reverse the conjugation of ubiquitin to targeted proteins. The importance of ubiquitin conjugation in many cellular processes suggests... aid436.table aid436.tbin
437 17 The apparent binding of compounds to Human ITPKC has been measured using differential scanning fluorimetry (DSF) technique. In this approach, the unfolding of the protein is monitored via a fluorescent hydrophobicity-sensing dye: SYPRO orange (Invitrogen). The hyperbolic curve of increase in fluorescence intensity versus temperature shows the unfolding process. The point of inflexion of this trace provides the measured parameter known as Tm. A difference in Tm between a control without the compou... aid437.table aid437.tbin
438 12277 Inflammatory disease requires that endothelial cells detect and amplify a pro-inflammatory signal. This results in the adherence, activation, and ultimately transmigration of lymphocytes at the site of damage. Drugs that block this process would significantly alleviate the symptoms of inflammation. Our assay detects an early event in this process, the nuclear translocation of the transcription factor, NFkappaB. Chronic inflammatory disease is believed to pose a tremendous medical burden in the de... aid438.table aid438.tbin
439 69 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute, TSRI Network: Molecular Library Screening Center Network (MLSCN) Proposal number 1 R03 MH076533-01 External Assay ID: (4.3) S1P3_AG_BLA_1536_EC50 Drun1 Name: S1P3 Agonist Dose-Response Potency Assay The biology of S1P receptor subtypes: Sphingosine 1-phosphate (S1P) influences heart rate [1] [2], coronary artery caliber, endothelial integrity, lung epitheli... aid439.table aid439.tbin
440 24304 University of New Mexico Assay Overview: Assay Support NIH 1R03MH076381-01 Assay for Formylpeptide Receptor Family Ligands PI: Bruce S. Edwards, Ph.D. Assay Background and Significance Formyl peptide receptors. The G-protein coupled formylpeptide receptor (FPR) was one of the originating members of the chemoattractant receptor superfamily (Le et al., 2002a; Oppenheim et al., 1991). N-formylated peptides such as fMLF are high affinity FPR ligands that trigger a variety of biologic activities i... aid440.table aid440.tbin
441 24304 University of New Mexico Assay Overview: Assay Support NIH 1R03MH076381-01 Assay for Formylpeptide Receptor Family Ligands PI: Bruce S. Edwards, Ph.D. Assay Background and Significance Formyl peptide receptors. The G-protein coupled formylpeptide receptor (FPR) was one of the originating members of the chemoattractant receptor superfamily (Le et al., 2002a; Oppenheim et al., 1991). N-formylated peptides such as fMLF are high affinity FPR ligands that trigger a variety of biologic activities... aid441.table aid441.tbin
442 6 The MKP-1 Phosphatase Dose Response Confirmation and Secondary Selectivity/Specificity Assay has been Developed and Run at the University of Pittsburgh Molecular Screening Center (PMLSC) part of the Molecular Library Screening Center Network (MLSCN). XO1 submission MH-76391 In vitro HTS assay for MKP-1, Assay Provider Dr. John S. Lazo, Department of Pharmacology at the University of Pittsburgh. The 107 protein tyrosine phosphatases (PTPs) found in the human genome are defined by the active site ... aid442.table aid442.tbin
443 6 The Cdc25B Phosphatase Secondary Selectivity Assay has been developed and run at the University of Pittsburgh Molecular Screening Center (PMLSC) part of the Molecular Library Screening Center Network (MLSCN). XO1 submission MH078959 Cdc25B catalytic domain in vitro assay, Assay Provider Dr. Marni Brisson, Department of Pharmacology at the University of Pittsburgh. The 107 protein tyrosine phosphatases (PTPs) found in the human genome are defined by the active site sequence C(X)5R(S/T), with X b... aid443.table aid443.tbin
444 10692 NCGC Assay Overview: The nuclear factor of activated T cells (NFAT) family of transcription factors has been found primarily in most immune system cells and some other non-immune cells. NFAT plays the immunomodulatory role, primarily in T-cell activation and differentiation. NFAT target genes are also involved in the regulation of apoptosis and differentiation in non-immune cell types. The NFAT sequence was engineered to the upstream of the ?-lactamase reporter gene and transfected into Jurkat c... aid444.table aid444.tbin
445 112066 NIH Molecular Libraries Screening Centers Network [MLSCN] NIH Chemical Genomics Center [NCGC] NCGC Assay Overview: Nuclear factor kappa-B (NF-kappa-B) plays an important role in normal B cell development and survival. Diffuse large B cell lymphoma (DLBCL) is the most commonly observed type of non-Hodgkin's lymphoma. Gene expression analysis has identified an activated B cell-like subtype of DLBCL (ABC-DLBCL) which expresses known NF-kappa-B target genes. In ABC-DLBCL cell lines this is due to hi... aid445.table aid445.tbin
446 10692 NCGC Assay Overview: The IL-6/STAT signaling pathway was assayed in ME-180 cervical carcinoma cells by a beta-lactamase reporter gene controlled by a STAT Inducible Element (SIE). This reporter is induced by IL-6 at 40 pM AC50 and inhibited by the pan JAK inhibitor, 2-(1,1-Dimethylethyl)-9-fluoro-3,6-dihydro-7H-benz[h]-imidaz[4,5-f]isoquinolin-7-one, at approximately 10 nM AC50. Plated cells were incubated overnight and on the following day, stimulated with 40 pM IL-6 for 5 hrs. The assay was... aid446.table aid446.tbin
447 68887 NIH Molecular Libraries Screening Centers Network [MLSCN] NIH Chemical Genomics Center [NCGC] Suzanne Walker [Harvard Medical School] NCGC Assay Overview: OGT is the sole enzyme that mediates the attachment of O-GlcNAc groups to serine and threonine residues in the nucleus and cytoplasm of eukaryotic cells, and no specific inhibitors are known. This type of glycosylation is involved in signal transduction and plays a key role in many essential cellular processes. High O-GlcNAc levels have been c... aid447.table aid447.tbin
448 64651 NIH Molecular Libraries Screening Centers Network [MLSCN] NIH Chemical Genomics Center [NCGC] David Williams [Illinois State University] NCGC Assay Overview: Schistosoma mansoni, a causative agent of schistosomiasis, resides in the bloodstream of their host up to 30 years without being eliminated by the host immune attack. One proposed survival mechanism is the production of an antioxidant "firewall" that neutralizes the oxidative assault of the host's immune attack. Schistosoma mansoni peroxire... aid448.table aid448.tbin
449 55727 Grant Proposal Number: 1 R03 MH076533-01 The biology of S1P receptor subtypes: Sphingosine 1-phosphate (S1P) influences heart rate [1] [2], coronary artery caliber, endothelial integrity, lung epithelial integrity [3] and lymphocyte recirculation [1] [4]-[6] through five related high affinity G-protein coupled receptors [7]. Inhibition of lymphocyte recirculation by nonselective S1P receptor agonists produces clinical immunosuppression preventing transplant rejection, but is associated with tran... aid449.table aid449.tbin
450 10949 NIH Molecular Libraries Screening Centers Network [MLSCN] NIH Chemical Genomics Center [NCGC] NCGC Assay Overview: The glucocorticoid receptor (GR) Redistribution assay (BioImage) enables the visualization of GR cytoplasmic to nuclear translocation by the use of a GR-GFP fusion. GR is normally cytosolic, however ligands such as dexamethasone, cause nuclear translocation where the protein binds to response elements and interacts with various co-factors to modulate transcription. Because both fu... aid450.table aid450.tbin
451 8728 NIH Molecular Libraries Screening Centers Network [MLSCN] NIH Chemical Genomics Center [NCGC] NCGC Assay Overview: The glucocorticoid receptor (GR) is a cytoplasmic receptor that belongs to the nuclear receptor family of ligand-dependent transcription factors. Upon glucocorticoid binding to its receptor, the glucocorticoid-GR complex translocates into the nucleus, where it binds as a dimer to specific DNA sequences (glucocorticoid response elements), enhancing or suppressing transcription of a w... aid451.table aid451.tbin
452 6 The MKP-3 Phosphatase Secondary Selectivity Assay has been developed and run at the University of Pittsburgh Molecular Screening Center (PMLSC) part of the Molecular Library Screening Center Network (MLSCN). XO1 submission MH-76390 In vitro HTS assay for MKP-3, Assay Provider Dr. John S. Lazo, Department of Pharmacology at the University of Pittsburgh. The 107 protein tyrosine phosphatases (PTPs) found in the human genome are defined by the active site sequence C(X)5R(S/T), with X being any ami... aid452.table aid452.tbin
453 63332 Molecular Library Screening Center Network (MLSCN) Penn Center for Molecular Discovery (PCMD) Assay Provider: Dr. Scott L. Diamond, University of Pennsylvania MLSCN Grant: X01-MH076406-01 Human liver cathepsin B (EC 3.4.22.1) is a lysosomal cysteine protease. There has been a recent resurgence of interest in cathepsin B due to research showing that proteolysis by this enzyme is required for the entry and replication of the Ebola and SARS viruses in human cells. Thus cathepsin B inhibitors have p... aid453.table aid453.tbin
454 9984 VCAM-1 (vascular cell adhesion molecule-1) mRNA and protein levels are potently induced by proinflammatory agents (TNFa, IL-1) resulting in enhanced VCAM-1 surface expression in HUVECs (human umbilical vein endothelial cells). VCAM-1 surface expression on HUVECs was evaluated in a cell-based plate reader screen. Small molecule attenuation of VCAM-1 surface expression on pooled HUVECs sub-maximally induced with TNF-alpha was measured by relative fluorescence intensity resulting from VCAM-1 immu... aid454.table aid454.tbin
455 9984 VCAM-1 (vascular cell adhesion molecule-1) mRNA and protein levels are potently induced by proinflammatory agents (TNFa, IL-1) resulting in enhanced VCAM-1 surface expression in HUVECs (human umbilical vein endothelial cells). VCAM-1 surface expression on HUVECs was evaluated in a cell-based plate reader screen. Small molecule enhancement of VCAM-1 surface expression on HUVECs sub-maximally induced with TNF-alpha was measured by relative fluorescence intensity resulting from VCAM-1 immunostain... aid455.table aid455.tbin
456 9984 VCAM-1 (vascular cell adhesion molecule-1) mRNA and protein levels are potently induced by proinflammatory agents (TNFa, IL-1) resulting in enhanced VCAM-1 surface expression in HUVECs (human umbilical vein endothelial cells). VCAM-1 surface expression on HUVECs was evaluated in a cell-based high-content imaging screen. Small molecule attenuation of VCAM-1 surface expression on HUVECs sub-maximally induced with TNF-alpha was measured by relative fluorescence intensity resulting from VCAM-1 imm... aid456.table aid456.tbin
457 9984 VCAM-1 (vascular cell adhesion molecule-1) mRNA and protein levels are potently induced by proinflammatory agents (TNFa, IL-1) resulting in enhanced VCAM-1 surface expression in HUVECs (human umbilical vein endothelial cells). VCAM-1 surface expression on HUVECs was evaluated in a cell-based high-content imaging screen. Small molecule enhancement of VCAM-1 surface expression on HUVECs sub-maximally induced with TNF-alpha was measured by relative fluorescence intensity resulting from VCAM-1 immu... aid457.table aid457.tbin
458 2 Differential static light scattering assay using StarGazer instrument from Harbinger Biotech: Protein samples are heated gradually, with light scattered by aggregated protein recorded as a function of temperature. aid458.table aid458.tbin
459 5 NIH Molecular Libraries Screening Centers Network [MLSCN] Emory Chemical Biology Discovery Center in MLSCN This assay was developed to determine the cytotoxic effects of small molecule compounds on A549 cells in a 96 well format after 48 hrs of exposure to test compounds. In this assay, cell viability is determined using the CellTiter-Blue reagent (Promega). The CellTiter-Blue cell viability assay provides a homogeneous, fluorometric method for estimating the number of viable cells present in mu... aid459.table aid459.tbin
460 57821 Molecular Library Screening Center Network (MLSCN) Penn Center for Molecular Discovery (PCMD) Assay Provider: Dr. Scott L. Diamond, University of Pennsylvania MLSCN Grant: X01-MH076406-01 Human liver cathepsin L (EC 3.4.22.15) is a lysosomal cysteine protease. Recent interest in cathepsin L has been generated by research showing that proteolysis by this enzyme is required for the entry and replication of the SARS and Ebola viruses in human cells. Thus cathepsin L inhibitors have potential as nov... aid460.table aid460.tbin
461 7113 Southern Research Molecular Libraries Screening Center (SRMLSC) Southern Research Institute (Birmingham, Alabama) NIH Molecular Libraries Screening Centers Network (MLSCN) Her2 (ErbB2) protein is over-expressed in breast and other solid tumors and is often mutated in patients with progressive disease. Her2 is a member of a family of four transmembrane tyrosine kinase receptors. It can form heterodimers with other members of this family to transduce extracellular growth signals via the MAP-Kin... aid461.table aid461.tbin
462 80 Inflammatory disease requires that endothelial cells detect and amplify a pro-inflammatory signal. This results in the adherence, activation, and ultimately transmigration of lymphocytes at the site of damage. Drugs that block this process would significantly alleviate the symptoms of inflammation. Our assay detects an early event in this process, the nuclear translocation of the transcription factor, NFkappaB. Chronic inflammatory disease is believed to pose a tremendous medical burden in the de... aid462.table aid462.tbin
463 56489 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute, TSRI Assay Provider: The Scripps Research Institute, TSRI Network: Molecular Library Screening Center Network (MLSCN) Compound cytotoxicity is an important parameter to measure when developing potential human therapeutics. Previously, in a separate report under Pubchem submission ID 364, we describe a high-throughput screening (HTS) campaign that was designed... aid463.table aid463.tbin
464 706 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute, TSRI Assay Provider: The Scripps Research Institute, TSRI Network: Molecular Library Screening Center Network (MLSCN) Compound cytotoxicity is an important parameter to measure when developing potential human therapeutics. Previously, in a separate report under Pubchem submission ID 364, we describe a high-throughput screening (HTS) campaign that was designed... aid464.table aid464.tbin
465 61609 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute, TSRI Assay Provider: The Burnham Institute Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal number: 1 X01 MH077633-01 Keywords: NF-kB, NF-kappaB, transcription factor, antigen receptor, Protein Kinase C-theta, PMA, ionomycin, luciferase, luminescence Description: Many cellular pathways leading to activation of NF-kB-family transcript... aid465.table aid465.tbin
466 508 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute, TSRI Assay Provider: The Scripps Research Institute, TSRI Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 1 R03 MH076533-01 The biology of S1P receptor subtypes Sphingosine 1-phosphate (S1P) influences heart rate (1,2), coronary artery caliber, endothelial integrity, lung epithelial integrity (3) and lymphocyte recirculati... aid466.table aid466.tbin
467 508 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute, TSRI Assay Provider: The Scripps Research Institute, TSRI Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 1 R03 MH076533-01 The biology of S1P receptor subtypes Sphingosine 1-phosphate (S1P) influences heart rate (1,2), coronary artery caliber, endothelial integrity, lung epithelial integrity (3) and lymphocyte recirculation... aid467.table aid467.tbin
468 508 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute, TSRI Assay Provider: The Scripps Research Institute, TSRI Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 1 R03 MH076533-01 The biology of S1P receptor subtypes Sphingosine 1-phosphate (S1P) influences heart rate (1,2), coronary artery caliber, endothelial integrity, lung epithelial integrity (3) and lymphocyte recirculation... aid468.table aid468.tbin
469 1040 Methylglyoxal (MG) is a highly reactive alpha-oxoaldehyde formed in cells primarily from the triose phosphate intermediates of glycolysis. It is the major physiologic substrate for the enzyme glyoxalase I, which is encoded by the GLOI gene. Together with glyoxalase II and a catalytic amount of GSH, glyoxalase I reduces methylglyoxal to D-lactate. In cells, methylglyoxal reacts almost exclusively with arginine residues to form the major methylglyoxal-derived epitope hydroimidazolone MG-H1 (N -ace... aid469.table aid469.tbin
470 1040 This assay measures the delay in larval development that is induced by a high glucose diet. The assay uses Drosophila embryos grown in 96 well plates containing high glucose food, which extends the normal developmental time from seven days to ten days. Candidate drugs are placed in the food in individual wells and scored for their ability to rescue the effects of the high glucose feeding on the developmental delay. Toxicity is determined by animal viability. aid470.table aid470.tbin
471 1040 This assay measures the expression levels of total IRS-1 in embryonic fibroblasts from PPARgamma2 knock out mice after treatment with different compounds. The assay measures IRS-1 with an ELISA kit from Invitrogen. aid471.table aid471.tbin
472 1040 This assay allows specific, quantitative detection of caspase-3 activity in cellular lysates of pericytes after induction of apoptosis in pericytes exposed to high (25mM) glucose. Caspase 3 activation plays a key role in initiation of cellular events during the early apoptotic process. The assay is based on spectophotometric detection of the chromophore p-nitroaniline (pNA) after cleavage from the labeled substrate DEVD-pNA (1). The free pNA is then quantified using a microtiter plate reader at 4... aid472.table aid472.tbin
473 1040 This ELISA measures the ability of test compounds to accelerate cleavage of the transmembrane protein IAP (integrin associated protein) when cells are grown in culture medium containing high (25mM) glucose. The assay uses a colorimetric assay to detect binding of an antibody whose affinity for IAP is increased following cleavage as a result of exposure of a neoepitope. The extent of antibody binding to cells grown in 25mM glucose in the presence or absence of the test compound is compared with th... aid473.table aid473.tbin
474 1040 This assay measures mitochondrial superoxide after 1 h exposure to 20 mM glucose. The assay uses a fluorescent probe that accumulates in mitochondria and compound activity is determined as a decrease in fluorescence compared to glucose alone. aid474.table aid474.tbin
475 406 Cultures of adult rat sensory neurons are assessed for levels of total axon outgrowth in response to sub-saturating neurotrophic growth factors under conditions of hyperglycemia. aid475.table aid475.tbin
476 30 This assay measures the rate death of cortical neurons in the presence of 15 mM glucose and 100 uM H2O2. We have already established that 15 mM glucose reduces cell viability by 75% under these conditions compared with 5 mM glucose, whereas without the presence of H202, neither 15 mM nor 5 mM glucose reduce viability. aid476.table aid476.tbin
477 1040 This assay measures the growth of endothelial vessel-like structures in a 3D collagen I matrix in response to exogenous stimulators. This assay has been modified to incorporate hyperglycemic injury (presence of 25 mM glucose) that causes endothelial cells to lose their ability to differentiate as they undergo apoptosis. The goal of this screen is to test compounds that protect cells from hyperglycemic injury and promote endothelial cell differentiation. The assay uses in-house developed software... aid477.table aid477.tbin
478 1040 This assay measures changes in calpain activity in heart microvascular endothelial in the presence of 25 mM D-glucose. The assay uses a membrane-permeable fluorescent substrate to measure calpain activity in situ. Calpain is a calcium-dependent protease implicated in diabetes and vascular disease. Hyperglycemia increases calpain activity in the vascular endothelium of the microcirculation with subsequent endothelial dysfunction and vascular inflammation. aid478.table aid478.tbin
479 1040 This assay measures the relative amount of fatty acyl-CoA bound to PPARalpha protein in the presence of 20 mM glucose. This assay utilizes a synthetic fluorescent fatty acyl-CoA analogue (BODIPY C-16-CoA) whose fluorescence intensity increases upon transfer from an aqueous environment (i.e. buffer in a cuvette) to a hydrophobic environment (i.e. ligand binding pocket). The BODIPY C-16-CoA is known to bind to PPARalpha with high affinity, and is displaced from the PPARalpha ligand binding pocket... aid479.table aid479.tbin
480 1040 This assay measures the degree of phosphorylation of c-Src (corresponding to the Tyrosine 418 residue of human c-Src) in human retinal endothelial cells stimulated with VEGF (25 ng/ml) in the presence of pharmacologic inhibitors. After treatment, cells are fixed and incubated with an antibody to phosphorylated c-Src. Cells are subsequently incubated with a secondary HRP-conjugated antibody with a chemiluminescent readout. aid480.table aid480.tbin
481 1040 This assay is designed to ascertain compounds that modulate insulin promoter activity in TRM6, a cell line derived from human fetal islets. The cells have been engineered to express PDX-1, NeuroD1, a tamoxifen-inducible form of E47 (E47MER), and the human insulin promoter driving eGFP. aid481.table aid481.tbin
482 1040 This assay measures the level of phosphorylated ERK1/2 in endothelial cells in the presence of 25 microg/mL S100b. The assay utilizes colorimetry to quantitate phosphorylated ERK1/2 in an in-situ cell-based ELISA. aid482.table aid482.tbin
483 9966 The mutation underlying Huntington's disease is an expansion of a polyglutamine tract in the N-terminus of the protein huntingtin (htt). Under nonpathogenic conditions, this stretch of glutamines range from 2 to 34 repeats, while greater than 37 repeats invariably leads to the disease. In an inducible mouse model of Huntington's disease, we found that abolishing mutant htt expression led to complete recovery of symptomatic mice (Yamamoto et al. 2000). Tightly linked to the symptomatic reversa... aid483.table aid483.tbin
484 196 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute, TSRI Assay Provider: The Scripps Research Institute, TSRI Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 1 R03 MH076533-01 The biology of S1P receptor subtypes: Sphingosine 1-phosphate (S1P) influences heart rate [1] [2], coronary artery caliber, endothelial integrity, lung epithelial integrity [3] and lymphocyte recirculati... aid484.table aid484.tbin
485 169238 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute (TSRI) Assay Provider: Hugh Rosen, TSRI Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 1 R03 MH076533-01 Grant Proposal PI: Germana Sanna, TSRI External Assay ID: S1P3_ANT_BLA_1536_%INH Name: Primary Cell-Based High Throughput Assay for Antagonists of the Sphingosine 1-Phosphate Receptor 3 (S1P3) Description: The biology of... aid485.table aid485.tbin
486 92 The mutation underlying Huntington's disease is an expansion of a polyglutamine tract in the N-terminus of the protein huntingtin (htt). Under nonpathogenic conditions, this stretch of glutamines range from 2 to 34 repeats, while greater than 37 repeats invariably leads to the disease. In an inducible mouse model of Huntington's disease, we found that abolishing mutant htt expression led to complete recovery of symptomatic mice (Yamamoto et al. 2000). Tightly linked to the symptomatic reversal wa... aid486.table aid486.tbin
487 12344 Inflammatory disease requires that endothelial cells detect and amplify a pro-inflammatory signal. This results in the adherence, activation, and ultimately transmigration of lymphocytes at the site of damage. Drugs that block this process would significantly alleviate the symptoms of inflammation. Our assay detects an early event in this process, the expression of E-selectin on the surface of endothelial cells, which is essential for lymphocyte adherence. Assay Principle. Cytokines such as tumor... aid487.table aid487.tbin
488 62108 One of our goals at the Penn Center for Molecular Discovery (PCMD) is to develop capabilities for screening multiple members of target classes, for example cysteine and serine proteases. Many HTS labs focus effort on one target of interest within a class due to resource and time constraints. A few compounds are then tested for selectivity against additional target class members during the hit-to-lead process. Our goal is to test the entire MLSCN compound library against multiple cysteine and seri... aid488.table aid488.tbin
489 96 This assay contains in vitro affinity data extracted from the literature for compounds tested against Influenza B virus (strain B/Lee/40). aid489.table aid489.tbin
490 14 This assay contains in vitro affinity data extracted from the literature for compounds tested against Influenza A virus (strain A/Tokyo/3/67 H2N2). aid490.table aid490.tbin
491 64 This assay contains in vitro affinity data extracted from the literature for compounds tested against Influenza A virus (strain A/Singapore/1/57 H2N2). aid491.table aid491.tbin
492 37 This assay contains in vitro affinity data extracted from the literature for compounds tested against Influenza A virus (A/Puerto Rico/8/34/Mount Sinai(H1N1)). aid492.table aid492.tbin
493 53 This assay contains in vitro affinity data extracted from the literature for compounds tested against Influenza A virus (A/Puerto Rico/8/34(H1N1)). aid493.table aid493.tbin
494 78 This assay contains in vitro affinity data extracted from the literature for compounds tested against Influenza A virus (A/tern/Australia/G70C/1975(H11N9)). aid494.table aid494.tbin
495 8 This assay contains in vitro affinity data extracted from the literature for compounds tested against Influenza A Subtype N2. aid495.table aid495.tbin
496 50 This assay contains in vitro affinity data extracted from the literature for compounds tested against Influenza B virus (strain B/Victoria/3/85). aid496.table aid496.tbin
497 2 This assay contains in vitro affinity data extracted from the literature for compounds tested against Influenza A virus (A/Shangdong/9/1993(H3N2)). aid497.table aid497.tbin
498 10 This assay contains in vitro affinity data extracted from the literature for compounds tested against Influenza B virus. aid498.table aid498.tbin
499 19 This assay contains in vitro affinity data extracted from the literature for compounds tested against Influenza B virus (B/Memphis/3/93). aid499.table aid499.tbin
500 2 This assay contains in vitro affinity data extracted from the literature for compounds tested against Influenza B virus (B/Nashville/6/89). aid500.table aid500.tbin
501 62029 Molecular Library Screening Center Network (MLSCN) Penn Center for Molecular Discovery (PCMD) Assay Provider: Dr. Scott L. Diamond, University of Pennsylvania MLSCN Grant: X01-MH076406-01 Human cathepsin S (EC 3.4.22.27) is a lysosomal cysteine protease that is expressed in antigen-presenting cells, especially dendritic cells, B-cells and macrophages. Cathepsin S plays a key role in the processing of antigenic peptides for presentation by MHC Class II molecules on the surface of antigen-presenti... aid501.table aid501.tbin
502 1 The apparent binding of compounds to Human AKR7A3 has been measured using differential scanning fluorimetry (DSF) technique. In this approach, the unfolding of the protein is monitored via a fluorescent hydrophobicity-sensing dye: SYPRO orange (Invitrogen). The hyperbolic curve of increase in fluorescence intensity versus temperature shows the unfolding process. The point of inflexion of this trace provides the measured parameter known as Tm. A difference in Tm between a control without the comp... aid502.table aid502.tbin
503 2 The apparent binding of compounds to Human RGS18 has been measured using differential scanning fluorimetry (DSF) technique. In this approach, the unfolding of the protein is monitored via a fluorescent hydrophobicity-sensing dye: SYPRO orange (Invitrogen). The hyperbolic curve of increase in fluorescence intensity versus temperature shows the unfolding process. The point of inflexion of this trace provides the measured parameter known as Tm. A difference in Tm between a control without the compo... aid503.table aid503.tbin
504 3 The apparent binding of compounds to Human RAC3 has been measured using differential scanning fluorimetry (DSF) technique. In this approach, the unfolding of the protein is monitored via a fluorescent hydrophobicity-sensing dye: SYPRO orange (Invitrogen). The hyperbolic curve of increase in fluorescence intensity versus temperature shows the unfolding process. The point of inflexion of this trace provides the measured parameter known as Tm. A difference in Tm between a control without the compou... aid504.table aid504.tbin
505 67 The apparent binding of compounds to Human PIM2 has been measured using differential scanning fluorimetry (DSF) technique. In this approach, the unfolding of the protein is monitored via a fluorescent hydrophobicity-sensing dye: SYPRO orange (Invitrogen). The hyperbolic curve of increase in fluorescence intensity versus temperature shows the unfolding process. The point of inflexion of this trace provides the measured parameter known as Tm. A difference in Tm between a control without the compou... aid505.table aid505.tbin
506 16 The apparent binding of compounds to Human NEK2 has been measured using differential scanning fluorimetry (DSF) technique. In this approach, the unfolding of the protein is monitored via a fluorescent hydrophobicity-sensing dye: SYPRO orange (Invitrogen). The hyperbolic curve of increase in fluorescence intensity versus temperature shows the unfolding process. The point of inflexion of this trace provides the measured parameter known as Tm. A difference in Tm between a control without the compou... aid506.table aid506.tbin
507 1 The apparent binding of compounds to Human MGC4172 has been measured using differential scanning fluorimetry (DSF) technique. In this approach, the unfolding of the protein is monitored via a fluorescent hydrophobicity-sensing dye: SYPRO orange (Invitrogen). The hyperbolic curve of increase in fluorescence intensity versus temperature shows the unfolding process. The point of inflexion of this trace provides the measured parameter known as Tm. A difference in Tm between a control without the com... aid507.table aid507.tbin
508 1 The apparent binding of compounds to Human MAT2A has been measured using differential scanning fluorimetry (DSF) technique. In this approach, the unfolding of the protein is monitored via a fluorescent hydrophobicity-sensing dye: SYPRO orange (Invitrogen). The hyperbolic curve of increase in fluorescence intensity versus temperature shows the unfolding process. The point of inflexion of this trace provides the measured parameter known as Tm. A difference in Tm between a control without the compo... aid508.table aid508.tbin
509 2 The apparent binding of compounds to Human HSD17B4 has been measured using differential scanning fluorimetry (DSF) technique. In this approach, the unfolding of the protein is monitored via a fluorescent hydrophobicity-sensing dye: SYPRO orange (Invitrogen). The hyperbolic curve of increase in fluorescence intensity versus temperature shows the unfolding process. The point of inflexion of this trace provides the measured parameter known as Tm. A difference in Tm between a control without the com... aid509.table aid509.tbin
510 5 The apparent binding of compounds to Human DHRS6 has been measured using differential scanning fluorimetry (DSF) technique. In this approach, the unfolding of the protein is monitored via a fluorescent hydrophobicity-sensing dye: SYPRO orange (Invitrogen). The hyperbolic curve of increase in fluorescence intensity versus temperature shows the unfolding process. The point of inflexion of this trace provides the measured parameter known as Tm. A difference in Tm between a control without the compo... aid510.table aid510.tbin
511 2 The apparent binding of compounds to Human CENTG1 has been measured using differential scanning fluorimetry (DSF) technique. In this approach, the unfolding of the protein is monitored via a fluorescent hydrophobicity-sensing dye: SYPRO orange (Invitrogen). The hyperbolic curve of increase in fluorescence intensity versus temperature shows the unfolding process. The point of inflexion of this trace provides the measured parameter known as Tm. A difference in Tm between a control without the comp... aid511.table aid511.tbin
512 9 The apparent binding of compounds to Human CBR3 has been measured using differential scanning fluorimetry (DSF) technique. In this approach, the unfolding of the protein is monitored via a fluorescent hydrophobicity-sensing dye: SYPRO orange (Invitrogen). The hyperbolic curve of increase in fluorescence intensity versus temperature shows the unfolding process. The point of inflexion of this trace provides the measured parameter known as Tm. A difference in Tm between a control without the compou... aid512.table aid512.tbin
513 23 The apparent binding of compounds to Human CSNK1G1 has been measured using differential scanning fluorimetry (DSF) technique. In this approach, the unfolding of the protein is monitored via a fluorescent hydrophobicity-sensing dye: SYPRO orange (Invitrogen). The hyperbolic curve of increase in fluorescence intensity versus temperature shows the unfolding process. The point of inflexion of this trace provides the measured parameter known as Tm. A difference in Tm between a control without the com... aid513.table aid513.tbin
514 90 The apparent binding of compounds to Human SLK has been measured using differential scanning fluorimetry (DSF) technique. In this approach, the unfolding of the protein is monitored via a fluorescent hydrophobicity-sensing dye: SYPRO orange (Invitrogen). The hyperbolic curve of increase in fluorescence intensity versus temperature shows the unfolding process. The point of inflexion of this trace provides the measured parameter known as Tm. A difference in Tm between a control without the compoun... aid514.table aid514.tbin
515 35 The apparent binding of compounds to Human MAP3K5 has been measured using differential scanning fluorimetry (DSF) technique. In this approach, the unfolding of the protein is monitored via a fluorescent hydrophobicity-sensing dye: SYPRO orange (Invitrogen). The hyperbolic curve of increase in fluorescence intensity versus temperature shows the unfolding process. The point of inflexion of this trace provides the measured parameter known as Tm. A difference in Tm between a control without the comp... aid515.table aid515.tbin
516 2 The apparent binding of compounds to Human BLVRA has been measured using differential scanning fluorimetry (DSF) technique. In this approach, the unfolding of the protein is monitored via a fluorescent hydrophobicity-sensing dye: SYPRO orange (Invitrogen). The hyperbolic curve of increase in fluorescence intensity versus temperature shows the unfolding process. The point of inflexion of this trace provides the measured parameter known as Tm. A difference in Tm between a control without the compo... aid516.table aid516.tbin
517 93 Inflammatory disease requires that endothelial cells detect and amplify a pro-inflammatory signal. This results in the adherence, activation, and ultimately transmigration of lymphocytes at the site of damage. Drugs that block this process would significantly alleviate the symptoms of inflammation. Our assay detects an early event in this process, the expression of E-selectin on the surface of endothelial cells, which is essential for lymphocyte adherence. Assay Principle. Cytokines such as tum... aid517.table aid517.tbin
518 64394 Sanford-Burnham Center for Chemical Genomics (SBCCG) Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Number: MH077602-01 Alkaline phosphatase (EC 3.1.3.1) (APs) catalyze the hydrolysis of phosphomonoesters, releasing phosphate and alcohol. APs are dimeric enzymes found in the most organism. In human, four isozymes of APs have been identified. Three isozymes are tissue-specific and the fourth one is tissue-nonsepeci... aid518.table aid518.tbin
519 272 University of New Mexico Assay Overview: Assay Support: NIH 1R03MH076381-01 Assay for Formylpeptide Receptor Family Ligands PI: Bruce S. Edwards, Ph.D. Assay Background and Significance Formyl peptide receptors. The G-protein coupled formylpeptide receptor (FPR) was one of the originating members of the chemoattractant receptor superfamily (Le et al., 2002a; Oppenheim et al., 1991). N-formylated peptides such as fMLF are high affinity FPR ligands that trigger a variety of biologic activities... aid519.table aid519.tbin
520 272 University of New Mexico Assay Overview Assay Support: NIH 1R03MH076381-01 Assay for Formylpeptide Receptor Family Ligands PI: Bruce S. Edwards, Ph.D. Assay Background and Significance Formyl peptide receptors. The G-protein coupled formylpeptide receptor (FPR) was one of the originating members of the chemoattractant receptor superfamily (Le et al., 2002a; Oppenheim et al., 1991). N-formylated peptides such as fMLF are high affinity FPR ligands that trigger a variety of biologic activities ... aid520.table aid520.tbin
521 114391 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Number: XO1 MH077603-01 Assay Provider: Dr. Tomas Mustelin, Sanford-Burnham Medical Research Institute Protein tyrosine phosphatases (PTPs), working with protein tyrosine kinases (PTKs), control the phosphorylation state of many proteins in the signal transduction pathways.... aid521.table aid521.tbin
522 64925 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute, TSRI Assay Provider: Orphagen Pharmaceuticals, San Diego, CA Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 1X01-MH077624-01 External Assay ID: SF1_AG_LUMI_1536_%ACT Name: Primary Cell-based High Throughput Screening assay for activators of the nuclear receptor Steroidogenic Factor 1 (SF-1) Description: Nuclear receptors ... aid522.table aid522.tbin
523 27 Human liver cathepsin B (EC 3.4.22.1) is a lysosomal cysteine protease. There has been a recent resurgence of interest in cathepsin B due to research showing that proteolysis by this enzyme is required for the entry and replication of the Ebola and SARS viruses in human cells. Thus cathepsin B inhibitors have potential as novel anti-viral agents. Cathepsin B is also implicated in cancer progression. Upregulation and secretion of this enzyme occurs in many types of tumors and correlates positive... aid523.table aid523.tbin
524 64925 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute, TSRI Assay Provider: Scripps Florida Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: NA External Assay ID: PKA_INH_Lumi_1536_%INH Name: Primary biochemical high-throughput screening assay for inhibitors of protein kinase A (PKA) activity Description: PKA is an ubiquitous serine/threonine protein kinase and belongs to the... aid524.table aid524.tbin
525 64925 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute, TSRI Assay Provider: Orphagen Pharmaceuticals, San Diego, CA Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 1X01-MH077624-01 Description: Nuclear receptors are a family of small molecule and hormone-regulated transcription factors that share conserved DNA-binding and ligand-binding domains. Small pharmacological compounds a... aid525.table aid525.tbin
526 67063 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] NCGC Assay Overview: The ubiquitin-proteasome pathway is present within all eukaryotic cells and plays roles in normal cellular functions and disease-related dysfunction. Proteins are tagged with a poly-ubiquitin chain that targets them for the proteasome, a multimeric protease, that degrades the protein into peptides and free ubiquitin. The proteasome has a key role in regulating cell cycle and growth ... aid526.table aid526.tbin
527 24085 University of New Mexico Assay Overview Assay Support 1X01MH078952-01 Small Molecule Inhibition of Staphylococcus aureus Virulence PI: Hattie D. Gresham, Ph.D. Background and Significance Quorum sensing is a cell-to-cell communication system that permits members of a bacterial population to coordinate their behavior dependent on cell density (for review see ref. Waters and Bassler, 2005). The mediators of this communication system are small, diffusible pheromones or autoinducers that are se... aid527.table aid527.tbin
528 23786 Assay Support: 1 X01 MH077638-01 MLSCN Assay for Allosteric Ligands for the VLA-4 Integrin PI: SKLAR, LARRY A University of New Mexico Assay Overview: This is a high throughput flow cytometry cell-based assay to identify small molecules that bind to an allosteric regulatory site on the integrin alpha-4-beta-1 heterodimer very late antigen (VLA-4). Such sites have the potential to control the affinity and conformation of integrins. Affinity states have been directly evaluated by a peptide ligan... aid528.table aid528.tbin
529 23786 Assay Support: 1 X01 MH077638-01 MLSCN Assay for Allosteric Ligands for the VLA-4 Integrin PI: SKLAR, LARRY A University of New Mexico Assay Overview: This is a high throughput flow cytometry cell-based assay to identify small molecules that bind to an allosteric regulatory site on the integrin alpha-4-beta-1 heterodimer very late antigen (VLA-4). Such sites have the potential to control the affinity and conformation of integrins. Affinity states have been directly evaluated by a peptide ligan... aid529.table aid529.tbin
530 11014 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] NCGC Assay Overview: The c-jun N-terminal kinase (JNK) family are serine/threonine protein kinases that phosphorylate c-jun, a component of the transcription factor protein-1 (AP-1). JNK kinases are members of the mitogen-activated protein kinase family including the extracellular regulated kinases and p38 kinases. Three JNK genes (JNK 1, 2, and 3) have been identified in humans so far. JNK1 and JNK2 h... aid530.table aid530.tbin
538 62137 Molecular Library Screening Center Network (MLSCN) Penn Center for Molecular Discovery (PCMD) Assay Provider: Scott L. Diamond, University of Pennsylvania MLSCN Grant: X01-MH076406-01 Target Complement factor C1s (EC 3.4.21.42) is a trypsin-like serine protease that is activated in one of the first steps in the classical complement cascade. Despite the essential role for the complement cascade in immune defense, unregulated activation leading to acute inflammation and tissue damage has been im... aid538.table aid538.tbin
539 65417 Southern Research Molecular Libraries Screening Center (SRMLSC) Southern Research Institute (Birmingham, Alabama) NIH Molecular Libraries Screening Centers Network (MLSCN) Assay Provider: Dr. Thomas S. Leyh, Albert Einstein College of Medicine of Yeshiva University Streptococcus pneumoniae takes the lives of nearly 4,000 people daily and antibiotic resistant strains are becoming an increasing problem. Because of this, the discovery of drugs targeting novel pathways has become increasingly impo... aid539.table aid539.tbin
540 1408 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] National Institutes of Environmental Health Sciences [NIEHS] National Toxicology Program [NTP] NCGC Assay Overview: We have developed a 1536-well cell-based assay for quantitative high throughput screening (qHTS) against a number of cell lines to determine in vitro cytotoxicity of small molecules. This particular assay uses the N2a cell line which is derived from mouse neuroblastoma. aid540.table aid540.tbin
541 1408 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] National Institutes of Environmental Health Sciences [NIEHS] National Toxicology Program [NTP] NCGC Assay Overview: We have developed a 1536-well cell-based assay for quantitative high throughput screening (qHTS) against a number of cell lines to determine in vitro cytotoxicity of small molecules. This particular assay uses the NIH 3T3 fibroblast cell line which is established from NIH Swiss mouse embry... aid541.table aid541.tbin
542 1408 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] National Institutes of Environmental Health Sciences [NIEHS] National Toxicology Program [NTP] NCGC Assay Overview: We have developed a 1536-well cell-based assay for quantitative high throughput screening (qHTS) against a number of cell lines to determine in vitro cytotoxicity of small molecules. This particular assay uses the HUV-EC-C cell line which is derived from normal human vascular endothelial c... aid542.table aid542.tbin
543 1408 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] National Institutes of Environmental Health Sciences [NIEHS] National Toxicology Program [NTP] NCGC Assay Overview: We have developed a 1536-well cell-based assay for quantitative high throughput screening (qHTS) against a number of cell lines to determine in vitro cytotoxicity of small molecules. This particular assay uses the H-4-II-E cell line which is derived from rat hepatoma. aid543.table aid543.tbin
544 1408 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] National Institutes of Environmental Health Sciences [NIEHS] National Toxicology Program [NTP] NCGC Assay Overview: We have developed a 1536-well cell-based assay for quantitative high throughput screening (qHTS) against a number of cell lines to determine in vitro cytotoxicity of small molecules. This particular assay uses the SH-SY5Y cell line which is derived from human neuroblastoma. aid544.table aid544.tbin
545 1408 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] National Institutes of Environmental Health Sciences [NIEHS] National Toxicology Program [NTP] NCGC Assay Overview: We have developed a 1536-well cell-based assay for quantitative high throughput screening (qHTS) against a number of cell lines to determine in vitro cytotoxicity of small molecules. This particular assay uses the renal proximal tubule cells which are derived from normal kidney cells fresh... aid545.table aid545.tbin
546 1408 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] National Institutes of Environmental Health Sciences [NIEHS] National Toxicology Program [NTP] NCGC Assay Overview: We have developed a 1536-well cell-based assay for quantitative high throughput screening (qHTS) against a number of cell lines to determine in vitro cytotoxicity of small molecules. This particular assay uses the kidney mesenchymal cell line which is derived from normal human renal glomer... aid546.table aid546.tbin
547 1280 NIH Molecular Libraries Screening Centers Network [MLSCN] Emory Chemical Biology Discovery Center in MLSCN MLSCN Grant Number: none Escherichia coli DnaK, a homolog of heat shock protein 70, has been shown to protect denature proteins from aggregation and promote their refolding by ATP hydrolysis. DnaK, along with its two co-cohort proteins DnaJ and GrpE, forms a microbial chaperone system that shelters microorganisms from environmental stresses such as temperature, osmotic, and pH changes, carb... aid547.table aid547.tbin
548 94 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute, TSRI Assay Provider: Scripps Florida Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: NA PKA is an ubiquitous serine/threonine protein kinase and belongs to the AGC kinase family. It has several functions in the cell, including regulation of immune response [1], transcription [2], cell cycle and apoptosis [3]. PKA is a cAMP de... aid548.table aid548.tbin
549 320 Southern Research Molecular Libraries Screening Center (SRMLSC) Southern Research Institute (Birmingham, Alabama) NIH Molecular Libraries Screening Centers Network (MLSCN) Assay Provider: Dr. Thomas S. Leyh, Albert Einstein College of Medicine of Yeshiva University Streptococcus pneumoniae takes the lives of nearly 4,000 people daily and antibiotic resistant strains are becoming an increasing problem. Because of this, the discovery of drugs targeting novel pathways has become increasingly impo... aid549.table aid549.tbin
550 320 Southern Research Molecular Libraries Screening Center (SRMLSC) Southern Research Institute (Birmingham, Alabama) NIH Molecular Libraries Screening Centers Network (MLSCN) Assay Provider: Dr. Thomas S. Leyh, Albert Einstein College of Medicine of Yeshiva University Streptococcus pneumoniae takes the lives of nearly 4,000 people daily and antibiotic resistant strains are becoming an increasing problem. Because of this, the discovery of drugs targeting novel pathways has become increasingly impo... aid550.table aid550.tbin
551 118 The MKP-1 HTS confirmation dose response assay has been developed to confirm actives identified in the MH-76391 In vitro HTS assay for MKP-1 inhibitors screened at the PMLSC AID #374.The MKP-1 Phosphatase HTS Dose Response Confirmation Assay has been Developed and Run at the University of Pittsburgh Molecular Screening Center (PMLSC) part of the Molecular Library Screening Center Network (MLSCN). XO1 submission MH-76391 In vitro HTS assay for MKP-1, Assay Provider Dr. John S. Lazo, Department of ... aid551.table aid551.tbin
552 19644 Southern Research Molecular Libraries Screening Center (SRMLSC) Southern Research Institute (Birmingham, Alabama) NIH Molecular Libraries Screening Centers Network (MLSCN) Assay Provider: Dr. Kim Lewis, Northeastern University Award: X01MH077622 There is considerable unmet need for novel antibiotics due to the rise of multi-drug resistant pathogens and the threat of engineered bioweapons. There is also an urgent need for novel antibiotics that can act against slow-growing or dormant pathogens a... aid552.table aid552.tbin
553 118 The in vitro MKP-3 Phosphatase dose response hit/probe assessment assay has been developed and run at the University of Pittsburgh Molecular Screening Center (PMLSC) part of the Molecular Library Screening Center Network (MLSCN)to follow up on actives identified in the MKP-3 HTS run at (Burham Institute) SDCCG center AID # 425 and the MKP-1 HTS run at the PMLSC AID # 374. XO1 submission MH-76390 In vitro HTS assay for MKP-3, Assay Provider Dr. John S. Lazo, Department of Pharmacology at the Unive... aid553.table aid553.tbin
555 65267 Southern Research Molecular Libraries Screening Center (SRMLSC) Southern Research Institute (Birmingham, Alabama) NIH Molecular Libraries Screening Centers Network (MLSCN) Assay Provider: Dr. Thomas S. Leyh, Albert Einstein College of Medicine of Yeshiva University Streptococcus pneumoniae takes the lives of nearly 4,000 people daily and antibiotic resistant strains are becoming an increasing problem. Because of this, the discovery of drugs targeting novel pathways has become increasingly impo... aid555.table aid555.tbin
556 65410 Southern Research Molecular Libraries Screening Center (SRMLSC) Southern Research Institute (Birmingham, Alabama) NIH Molecular Libraries Screening Centers Network (MLSCN) Assay Provider: Dr. Thomas S. Leyh, Albert Einstein College of Medicine of Yeshiva University Streptococcus pneumoniae takes the lives of nearly 4,000 people daily and antibiotic resistant strains are becoming an increasing problem. Because of this, the discovery of drugs targeting novel pathways has become increasingly impo... aid556.table aid556.tbin
557 318 Southern Research Molecular Libraries Screening Center (SRMLSC) Southern Research Institute (Birmingham, Alabama) NIH Molecular Libraries Screening Centers Network (MLSCN) Assay Provider: Dr. Thomas S. Leyh, Albert Einstein College of medicine of Yeshiva University Streptococcus pneumonia (SP) takes the lives of nearly 4,000 people daily and antibiotic resistant strains are becoming an increasing problem. Because of this, the discovery of drugs targeting novel pathways such as the mevalonate p... aid557.table aid557.tbin
558 58 The MKP-1 dose response Active/Probe assessment-DTT assay has been developed to evaluate the effects of increased DTT concentration on the MKP-1 inhibition of actives identified in the MH-76391 In vitro MKP-1 HTS assay AID #374, and subsequently confirmed in the HTS dose response confirmation assay AID #551. Protein tyrosine phosphatases have an active site cysteine that is very susceptible to inactivation by oxidation. In addition, a number of compounds such as quinone-like compounds are capabl... aid558.table aid558.tbin
559 62237 Molecular Library Screening Center Network (MLSCN) Penn Center for Molecular Discovery (PCMD) Assay Provider: Dr. Arkady Mustaev, Public Health Research Institute, Newark, NJ MLSCN Grant: RO3 MH076325-01 DNA-directed RNA polymerase (EC 2.7.7.6) is responsible for bacterial RNA synthesis and as such is essential for bacterial gene expression. Owing to its central role in DNA transcription, the enzyme RNA polymerase is the target of various natural antibiotics. The best known is rifampicin, a pot... aid559.table aid559.tbin
560 64925 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute, TSRI Assay Provider: Orphagen Pharmaceuticals, San Diego, CA Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 1X01-MH077624-01 External Assay ID: RORA_AG_Lumi_1536_%ACT Name: Primary Cell-based High Throughput Screening assay for activators of the Retinoic Acid Receptor-related orphan receptor A (RORA) Description: Nuclear r... aid560.table aid560.tbin
561 64925 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute, TSRI Assay Provider: Orphagen Pharmaceuticals, San Diego, CA Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 1X01-MH077624-01 Nuclear receptors are a family of small molecule and hormone-regulated transcription factors that share conserved DNA-binding and ligand-binding domains. Small pharmacological compounds able to bind ... aid561.table aid561.tbin
562 58 The MKP-1 dose response Active/Probe assessment-Catalase assay has been developed to evaluate the effects of adding 100 U/mL of Catalase on the MKP-1 inhibition of actives identified in the MH-76391 In vitro MKP-1 HTS assay AID #374, and subsequently confirmed in the HTS dose response confirmation assay AID #551. Protein tyrosine phosphatases have an active site cysteine that is very susceptible to inactivation by oxidation. In addition, a number of compounds such as quinone-like compounds are ca... aid562.table aid562.tbin
563 58 The MKP-1 dose response assay Active/Probe Assessment Assay - Reproducibility testing has been developed to test the reproducibility of MKP-1 inhibitors identified in the MH-76391 In vitro HTS assay for MKP-1 inhibitors AID #374 and subsequently confirmed in the MKP-1 HTS dose response confirmation assay AID # 551.The MKP-1 Phosphatase dose response assay Active/Probe Assessment Assay - Reproducibility testing has been Developed and Run at the University of Pittsburgh Molecular Screening Center (... aid563.table aid563.tbin
564 58 The MKP-3 dose response Active/Probe assessment-Catalase assay has been developed to evaluate the effects of adding 100 U/mL of Catalase on the MKP-3 inhibition of actives identified in the MKP-3 HTS run at (Burham Institute) SDCCG center AID 425 and the MKP-1 HTS run at the PMLSC AID 374, and subsequently confirmed in the MKP-3 & MKP-1 HTS dose response confirmation assays AID's 553 & 551. Protein tyrosine phosphatases have an active site cysteine that is very susceptible to inactivation by oxid... aid564.table aid564.tbin
565 65239 The HIV-1 RT-RNase H assay was submitted by Dr. Michael Parniak of the University of Pittsburgh, MLSCN XO1 MH077605, and the HTS was developed and screened at the University of Pittsburgh Molecular Library Screening Center (PMLSC). The rapid development of HIV-1 resistance to antiretroviral agents is a major clinical problem. This troubling phenomenon has been observed with each class of current clinically used anti-HIV agents. An increasingly serious clinical problem is the emergence of multi-d... aid565.table aid565.tbin
566 58 The MKP-3 dose response assay Active/Probe Assessment Assay - Reproducibility testing has been developed to test the reproducibility of MKP-3 inhibitors of actives identified in the MKP-3 HTS run at (Burham Institute) SDCCG center AID 425 and the MKP-1 HTS run at the PMLSC AID 374, and subsequently confirmed in the MKP-3 & MKP-1 HTS dose response confirmation assays AID's 553 & 551. The in vitro MKP-3 Phosphatase dose response hit/probe assessment reproducibility testing assay has been developed ... aid566.table aid566.tbin
567 64925 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute, TSRI Assay Provider: Scripps Florida Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 1 R03 MH076345-01 External Assay ID: 5HT1a_AG_BLA_1536_%ACT Name: Primary HTS assay for 5-Hydroxytryptamine (Serotonin) Receptor Subtype 1a (5HT1a) agonists Description: Widely expressed in the human brain, 5-hydroxytryptamine (5-HT, seroto... aid567.table aid567.tbin
568 82559 Sanford-Burnham Center for Chemical Genomics (SBCCG) Sanford-Burnham Medical Research Institute (San Diego, CA) NIH Molecular Libraries Screening Centers Network (MLSCN) Over-expression of molecular chaperones occurs commonly in cancers and provides protection from a wide variety of cellular stresses, both endogenous and iatrogenic. Molecular chaperones also play important roles in maintaining the activity of several signal-transducing proteins and transcriptions factors involved in malignant tr... aid568.table aid568.tbin
569 84 The Cdc25B Phosphatase HTS dose response confirmation has been developed to confirm actives identified in the Cdc25B HTS AID 368, screened at the University of Pittsburgh Molecular Screening Center (PMLSC) part of the Molecular Library Screening Center Network (MLSCN). XO1 submission MH078959 Cdc25B catalytic domain in vitro assay, Assay Provider Dr. Marni Brisson, Department of Pharmacology at the University of Pittsburgh. The 107 protein tyrosine phosphatases (PTPs) found in the human genome ... aid569.table aid569.tbin
570 64925 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute, TSRI Assay Provider: Florida Atlantic University Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 1 X01 MH078948-01 Osteoarthritis (OA) is an age-related debilitating disease affecting more than 80% of people over the age of 75, caused by the destruction of articular cartilage. The major components of the cartilage extracellul... aid570.table aid570.tbin
571 64925 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute, TSRI Assay Provider: Albany Medical College Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 1 R03 MH076345-01 External Assay ID: 5HT1E_ANT_BLA_1536_%INH Name: Primary Cell Based High Throughput Screening Assay for Antagonists of the 5-Hydroxytryptamine Receptor Subtype 1E (5HT1E) Description: The neurotransmitter, serotoni... aid571.table aid571.tbin
572 9033 Southern Research Molecular Libraries Screening Center (SRMLSC) Southern Research Institute (Birmingham, Alabama) NIH Molecular Libraries Screening Centers Network (MLSCN) (Proposal number 1R03MH076408-012) Submitted by Gabriela Chiosis of the Memorial Sloan-Kettering Institute for Cancer Research Compounds that inhibit tumor cell growth may have cytotoxic or cytostatic effects and vary widely in structure and mechanism of action. Cancer chemotherapeutic drugs inhibit tumor cell growth by disru... aid572.table aid572.tbin
573 65120 Southern Research Molecular Libraries Screening Center (SRMLSC) Southern Research Institute (Birmingham, Alabama) NIH Molecular Libraries Screening Centers Network (MLSCN) Assay Provider: Dr. Kim Lewis, Northeastern University Award: X01MH077622 There is considerable unmet need for novel antibiotics due to the rise of multi-drug resistant pathogens and the threat of engineered bioweapons. There is also an urgent need for novel antibiotics that can act against slow-growing or dormant pathogens ... aid573.table aid573.tbin
574 64925 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute, TSRI Assay Provider: Albany Medical College Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 1 R03 MH076345-01 The neurotransmitter, serotonin (5HT, 5-hydroxytryptamine) is important in a large number of neurological behaviors including of mood[1], appetite[2], cognition[3], pain[4] and memory[5]. The serotonin receptors are ... aid574.table aid574.tbin
575 9993 Southern Research Molecular Libraries Screening Center (SRMLSC) Southern Research Institute (Birmingham, Alabama) NIH Molecular Libraries Screening Centers Network (MLSCN) Assay Provider: Dr. Zhican Qu, Southern Research Institute Angiogenesis is a process of new blood vessel formation. Endothelial cell proliferation is an essential step during the angiogenesis process and is involved in many human diseases including cancer, diabetic retinopathy, and rheumatoid arthritis. Targeting endothelia... aid575.table aid575.tbin
576 23785 Assay Support: 1 X01 MH077638-01 MLSCN Assay for Allosteric Ligands for the VLA-4 Integrin PI: SKLAR, LARRY A University of New Mexico Assay Overview: This is a high throughput flow cytometry cell-based assay to identify auto-fluorescence of small molecules. The fluorescence measured is from the compounds potential internal and external association with cells. This screen establishes a baseline used during screening of allosteric regulators of the integrin alpha-4-beta-1 heterodimer very late... aid576.table aid576.tbin
577 65239 The HTS assay to identify Inhibitors of West Nile Virus NS2bNS3 Proteinase was proposed by Dr Alex Strongin of the Burnham Institute XO1-MH077601, and was developed and screened at the University of Pittsburgh Molecular Library Screening Center part of the Molecular Library Screening Center Network (MLSCN). Extracted from the XO1-MH077601 Proposal submitted by Dr. Alex Strongin, Burnham Institute: West Nile virus, a member of the Flaviviridae family, was first isolated in 1937 in the West Nile... aid577.table aid577.tbin
578 960 This assay contains in vitro affinity data extracted from the literature for compounds tested against EGF-R Tyrosine Kinase Homo sapiens. aid578.table aid578.tbin
579 47 The MKP-1 dose response assay SAR support Assay - has been developed to test the activity of a series Analog compounds synthesized by the PMLSC Chemistry Core based on MKP-1 inhibitors identified in the MH-76391 In vitro HTS assay for MKP-1 inhibitors AID 374 and subsequently confirmed in the MKP-1 HTS dose response confirmation assay AID 551. The MKP-1 Phosphatase dose response SAR support assay has been Developed and Run at the University of Pittsburgh Molecular Screening Center (PMLSC) part o... aid579.table aid579.tbin
580 9991 Southern Research Molecular Libraries Screening Center (SRMLSC) Southern Research Institute (Birmingham, Alabama) NIH Molecular Libraries Screening Centers Network (MLSCN) (Proposal number 1X01-MH077620-01) Submitted by Gary A. Piazza of Southern Research Institute Compounds that inhibit cell growth may have cytotoxic or cytostatic effects and vary widely in structure and mechanism of action. Most cancer chemotherapeutic drugs inhibit tumor cell growth by disrupting cell division, which often r... aid580.table aid580.tbin
581 62105 Molecular Library Screening Center Network (MLSCN) Penn Center for Molecular Discovery (PCMD) Assay Provider: Dr. Scott L. Diamond, University of Pennsylvania MLSCN Grant: X01-MH076406-01 Cathepsin G (EC 3.4.21.20) is a chymotrypsin-like serine protease that is secreted from neutrophils. Disregulated cathepsin G activity is implicated in the progression of various chronic inflammatory diseases such as asthma and chronic pulmonary obstructive disease. Thus cathepsin G inhibitors represent useful... aid581.table aid581.tbin
583 135404 Sanford-Burnham Center for Chemical Genomics (SBCCG) Sanford-Burnham Medical Research Institute (San Diego, CA) NIH Molecular Libraries Screening Centers Network (MLSCN) MLSCN Grant: XO1 MH079863-01 Over-expression of molecular chaperones occurs commonly in cancers and provides protection from a wide variety of cellular stresses, both endogenous and iatrogenic. Molecular chaperones also play important roles in maintaining the activity of several signal-transducing proteins and transcriptions fac... aid583.table aid583.tbin
584 70699 NIH Molecular Libraries Screening Centers Network [MLSCN] NIH Chemical Genomics Center [NCGC] MLSCN Grant: 1 X01 MH079825-01 PI Name: Shoichet, Brian K. NCGC Assay Overview: This aggregation profiling approach exploits the sensitivity of aggregate formation to detergent. Inhibition of b-lactamase is measured in the presence and absence of 0.01% Triton X-100. This particular assay had the prescence of 0.01% Triton X-100. See Pubchem assay "Promiscuous and Specific Inhibitors of AmpC Beta-Lactama... aid584.table aid584.tbin
585 70699 NIH Molecular Libraries Screening Centers Network [MLSCN] NIH Chemical Genomics Center [NCGC] MLSCN Grant: 1 X01 MH079825-01 PI Name: Shoichet, Brian K. NCGC Assay Overview: This aggregation profiling approach exploits the sensitivity of aggregate formation to detergent. Inhibition of b-lactamase is measured in the presence and absence of 0.01% Triton X-100. See Pubchem assay "Promiscuous and Specific Inhibitors of AmpC Beta-Lactamase (assay with detergent)" for related screen. Compounds that i... aid585.table aid585.tbin
586 112 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute, TSRI Assay Provider: The Burnham Institute Network: Molecular Library Screening Center Network (MLSCN) Proposal number 1X01-MH077633-01 External Assay ID: NFkB_INH_LUMI_1536_IC50 Name: Dose-response cell-based assay for chemical inhibitors of antigen receptor-induced NF-kappaB activation Description: Many cellular pathways leading to activation of NF-kB-fam... aid586.table aid586.tbin
587 59094 NIH Molecular Libraries Screening Centers Network [MLSCN] NIH Chemical Genomics Center [NCGC] NCGC Assay Overview: The rate of false hits is dramatically reduced by the qHTS approach (Inglese et al, PNAS, 103, 1147 (2006)) as spurious high- or low-response wells are quickly revealed when plotted in the context of concentration response. Occasionally, the artifactual effect may originate from the compound#s own spectral or other biophysical properties which generally tend to track its assay conce... aid587.table aid587.tbin
588 59094 NIH Molecular Libraries Screening Centers Network [MLSCN] NIH Chemical Genomics Center [NCGC] NCGC Assay Overview: The rate of false hits is dramatically reduced by the qHTS approach (Inglese et al, PNAS, 103, 1147 (2006)) as spurious high- or low-response wells are quickly revealed when plotted in the context of concentration response. Occasionally, the artifactual effect may originate from the compound#s own spectral or other biophysical properties which generally tend to track its assay conce... aid588.table aid588.tbin
589 59094 NIH Molecular Libraries Screening Centers Network [MLSCN] NIH Chemical Genomics Center [NCGC] NCGC Assay Overview: The rate of false hits is dramatically reduced by the qHTS approach (Inglese et al, PNAS, 103, 1147 (2006)) as spurious high- or low-response wells are quickly revealed when plotted in the context of concentration response. Occasionally, the artifactual effect may originate from the compounds own spectral or other biophysical properties which generally tend to track its assay concen... aid589.table aid589.tbin
590 59094 NIH Molecular Libraries Screening Centers Network [MLSCN] NIH Chemical Genomics Center [NCGC] NCGC Assay Overview: The rate of false hits is dramatically reduced by the qHTS approach (Inglese et al, PNAS, 103, 1147 (2006)) as spurious high- or low-response wells are quickly revealed when plotted in the context of concentration response. Occasionally, the artifactual effect may originate from the compounds own spectral or other biophysical properties which generally tend to track its assay concen... aid590.table aid590.tbin
591 59094 NIH Molecular Libraries Screening Centers Network [MLSCN] NIH Chemical Genomics Center [NCGC] NCGC Assay Overview: The rate of false hits is dramatically reduced by the qHTS approach (Inglese et al, PNAS, 103, 1147 (2006)) as spurious high- or low-response wells are quickly revealed when plotted in the context of concentration response. Occasionally, the artifactual effect may originate from the compounds own spectral or other biophysical properties which generally tend to track its assay concen... aid591.table aid591.tbin
592 59094 NIH Molecular Libraries Screening Centers Network [MLSCN] NIH Chemical Genomics Center [NCGC] MLSCN Grant number: none NCGC Assay Overview: The rate of false hits is dramatically reduced by the qHTS approach (Inglese et al, PNAS, 103, 1147 (2006)) as spurious high- or low-response wells are quickly revealed when plotted in the context of concentration response. Occasionally, the artifactual effect may originate from the compounds own spectral or other biophysical properties which generally tend ... aid592.table aid592.tbin
593 59093 NIH Molecular Libraries Screening Centers Network [MLSCN] NIH Chemical Genomics Center [NCGC] NCGC Assay Overview: The rate of false hits is dramatically reduced by the qHTS approach (Inglese et al, PNAS, 103, 1147 (2006)) as spurious high- or low-response wells are quickly revealed when plotted in the context of concentration response. Occasionally, the artifactual effect may originate from the compound's own spectral or other biophysical properties which generally tend to track its assay conce... aid593.table aid593.tbin
594 59094 NIH Molecular Libraries Screening Centers Network [MLSCN] NIH Chemical Genomics Center [NCGC] NCGC Assay Overview: The rate of false hits is dramatically reduced by the qHTS approach (Inglese et al, PNAS, 103, 1147 (2006)) as spurious high- or low-response wells are quickly revealed when plotted in the context of concentration response. Occasionally, the artifactual effect may originate from the compound#s own spectral or other biophysical properties which generally tend to track its assay conce... aid594.table aid594.tbin
595 70699 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] NCGC Assay Overview: Hsp90 (heat shock protein 90) is the essential molecular chaperone and it accounts for 1-2% of all cytosolic proteins and is critical for the activity of diverse cellular proteins that are involved in a variety of cellular processes, including development, cell cycle, and steroid hormone signaling. Its client proteins include signaling kinases such as IGF1R, Akt, v-Src, Raf-1; regul... aid595.table aid595.tbin
596 70699 NIH Molecular Libraries Screening Centers Network [MLSCN] NIH Chemical Genomics Center [NCGC] MLSCN Grant: 1 X01 MH079825-01 Assay Provider: Jeffrey A. Kuret, Ohio State University NCGC Assay Overview: Tau monomers form filaments in vitro in the presence of anionic detergents or fatty acids. The dye Thioflavine S (ThS) binds to tau filaments and upon binding, increases in fluorescence several fold. Small molecules that displace ThS binding or prevent filament binding are identified by a reduct... aid596.table aid596.tbin
597 68401 NIH Molecular Libraries Screening Centers Network [MLSCN] NIH Chemical Genomics Center [NCGC] MLSCN Grant: 1 X01 MH079860-01 Assay Provider: Elisabeth D. Martinez, University of Texas SW Medical Center NCGC Assay Overview: The Locus Derepression assay detects the derepression of a GFP reporter that is stably integrated in a region of the genome of murine c127i mammary cells that is presumably silenced. GFP transcription in this construct is controlled by a CMV promoter, which normally is stron... aid597.table aid597.tbin
598 85210 Southern Research Molecular Libraries Screening Center (SRMLSC) Southern Research Institute (Birmingham, Alabama) NIH Molecular Libraries Screening Centers Network (MLSCN) (Proposal number 1X01-MH077620-01) Submitted by Gary A. Piazza of Southern Research Institute Compounds that inhibit cell growth may have cytotoxic or cytostatic effects and vary widely in structure and mechanism of action. Most cancer chemotherapeutic drugs inhibit tumor cell growth by disrupting cell division, which often r... aid598.table aid598.tbin
599 357 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute, TSRI Assay Provider: Orphagen Pharmaceuticals, San Diego, CA Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal number 1X01-MH077624-01 External Assay ID: RORA_INH_Lumi_1536_CS_IC50 Name: Counterscreen for inhibitors of the nuclear receptor Steroidogenic Factor 1 (SF-1): A cell-based dose-response assay for inhibition of the RAR-rel... aid599.table aid599.tbin
600 359 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute, TSRI Assay Provider: Orphagen Pharmaceuticals, San Diego, CA Network: Molecular Library Screening Center Network (MLSCN) Grant proposal number 1X01-MH077624-01 External Assay ID: SF-1_INH_Lumi_1536_IC50 Name: Dose-response cell-based assay for inhibitors of the nuclear receptor Steroidogenic Factor 1 (SF-1) Description: Nuclear receptors are a family of sma... aid600.table aid600.tbin
601 9991 Southern Research Molecular Libraries Screening Center (SRMLSC) Southern Research Institute (Birmingham, Alabama) NIH Molecular Libraries Screening Centers Network (MLSCN) (Proposal number 1X01-MH077620-01) Submitted by Dr. Gary A. Piazza of Southern Research Institute. Drug resistance, whether intrinsic or acquired, is a major clinical obstacle, which limits the efficacy of cancer chemotherapy. Multi-drug resistance (MDR) is a phenomenon by which tumor cells display or develop resistance to a ... aid601.table aid601.tbin
602 85210 Southern Research Molecular Libraries Screening Center (SRMLSC) Southern Research Institute (Birmingham, Alabama) NIH Molecular Libraries Screening Centers Network (MLSCN) (Proposal number 1X01-MH077620-01) Submitted by Dr. Gary A. Piazza of Southern Research Institute. Drug resistance, whether intrinsic or acquired, is a major clinical obstacle, which limits the efficacy of cancer chemotherapy. Multi-drug resistance (MDR) is a phenomenon by which tumor cells display or develop resistance to a ... aid602.table aid602.tbin
603 70699 NIH Molecular Libraries Screening Centers Network [MLSCN] NIH Chemical Genomics Center [NCGC] MLSCN Grant: 1 X01 MH077636-01 Assay Provider: Charles McHenry, University of Colorado NCGC Assay Overview: E. coli DNA polymerase III holoenzyme complex was assayed for DNA production by fluorescent detection of the double-stranded DNA product with PicoGreen dye. The holoenzyme complex was reconstituted from the following purified protein components: DNA Polymerase III, beta subunit processivity facto... aid603.table aid603.tbin
604 59805 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute, TSRI Assay Provider: Scripps Florida Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: NA External Assay ID: Rhok2_INH_LUMI_1536_%INH Name: Primary biochemical high-throughput screening assay for inhibitors of Rho kinase 2 (Rhok2) Description: Rho-Kinase is a serine/threonine kinase that is involved in the regulation of smoo... aid604.table aid604.tbin
605 70699 NIH Molecular Libraries Screening Centers Network [MLSCN] NIH Chemical Genomics Center [NCGC] Assay Provider: Eric Brown, McMaster University NCGC Assay Overview: YjeE is an essential E.coli protein of unknown function that binds adenosine diphosphate (ADP). A complex of YjeE and BODIPY Texas Red-labeled ADP probe (Invitrogen, catalog number A22359) is incubated with library members. Inhibitors of YjeE-ADP binding are detected by a decrease in the fluorescence polarization (FP) of the fluoropho... aid605.table aid605.tbin
606 51943 LYP, a lymphocyte specific protein tyrosine phosphatase that plays a critical regulatory role in T cell receptor signaling, is encoded by the PTPN22 gene. A single-nucleotide polymorphism in PTPN22 is associated with a number of autoimmune disorders, including type 1 diabetes, rheumatoid arthritis, juvenile rheumatoid arthritis, systemic lupus erythematosus and Grave#s disease. The autoimmunity-predisposing allele is a gain-of-function mutant suggesting that its effect could be eliminated by a sp... aid606.table aid606.tbin
607 9202 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] NCGC Assay Overview: The cyclic nucleotide phosphodiesterases (PDEs) are proteins that catalyze hydrolysis of 3', 5'-cyclic nucleotides, such as cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP), to their corresponding 5'-nucleotide monophosphates. These enzymes play an important role in controlling cellular concentrations of cyclic nucleotides and have a central role in a ... aid607.table aid607.tbin
608 3819 Sanford-Burnham Center for Chemical Genomics (SBCCG) Sanford-Burnham Medical Research Institute (San Diego, CA) NIH Molecular Libraries Screening Centers Network (MLSCN) Peptidylprolyl isomerases (PPIases) are a group of cytosolic enzymes initially characterized by their ability to catalyze the cis-trans isomerization of cis-peptidylprolyl bonds. The cis-trans interconversion accelerated by PPIases is significant for protein folding because cis proline introduces critical bends within the protei... aid608.table aid608.tbin
609 3316 MKP-3 Chemical Complementation HTS Developed and Run at the University of Pittsburgh Molecular Screening Center (PMLSC) as part of the Molecular Library Screening Center Network (MLSCN). XO1 submission MH76330 Chemical Complementation Assay for MKP-3, Assay Provider Dr. John Lazo, Department of Pharmacology at the University of Pittsburgh. The activities of mitogen-activated protein kinases (MAPK) are negatively regulated by mitogen activated protein kinase (MAPK) phosphatases (MKPs). To date, ... aid609.table aid609.tbin
610 273 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute, TSRI Assay Provider: Orphagen Pharmaceuticals, San Diego, CA Network: Molecular Library Screening Center Network (MLSCN) Proposal number 1X01-MH077624-01 External Assay ID: RORA_INH_Lumi_1536_IC50 Name: Dose-response cell-based assay for inhibitors of the Retinoic Acid Receptor-related orphan receptor A (RORA) Description: Nuclear receptors are a family o... aid610.table aid610.tbin
611 273 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute, TSRI Assay Provider: Orphagen Pharmaceuticals, San Diego, CA Network: Molecular Library Screening Center Network (MLSCN) Proposal number 1X01-MH077624-01 External Assay ID: SF1_INH_Lumi_1536_CS_IC50 Name: Counterscreen for inhibitors of the Retinoic Acid Receptor-related orphan receptor A (RORA): A cell-based dose-response assay for inhibition of the Steroi... aid611.table aid611.tbin
612 61609 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute, TSRI Assay Provider: Scripps Florida Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 1 R03 MH076345-01 External Assay ID: 5HT1a_ANT_BLA_1536_%INH Name: Primary HTS assay for 5-Hydroxytryptamine (Serotonin) Receptor Subtype 1a (5HT1a) antagonists Description: Widely expressed in the human brain, 5-hydroxytryptamine (5-HT, ... aid612.table aid612.tbin
613 346 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute, TSRI Assay Provider: Scripps Florida Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 1 R03 MH076345-01 Assay Description: Widely expressed in the human brain, 5-hydroxytryptamine (5-HT, serotonin) receptors have been shown to have an important role in depression as well as other cognitive and metabolic disorders [1, 2]. Agoni... aid613.table aid613.tbin
614 20540 Sanford-Burnham Center for Chemical Genomics (SBCCG) Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) NIH Molecular Libraries Screening Centers Network (MLSCN) Alkaline phosphatase (EC 3.1.3.1) (APs) catalyze the hydrolysis of phosphomonoesters, releasing phosphate and alcohol. APs are dimeric enzymes found in most of the organisms. In human, four isozymes of APs have been identified. Three isozymes are tissue-specific and the fourth one is tissue-nonsepecifc, named TNAP. TNAP d... aid614.table aid614.tbin
615 20540 Sanford-Burnham Center for Chemical Genomics (SBCCG) Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) NIH Molecular Libraries Screening Centers Network (MLSCN) Alkaline phosphatase (EC 3.1.3.1) (APs) catalyze the hydrolysis of phosphomonoesters, releasing phosphate and alcohol. APs are dimeric enzymes found in most of the organisms. In human, four isozymes of APs have been identified. Three isozymes are tissue-specific and the fourth one is tissue-nonsepecifc, named TNAP. TNAP d... aid615.table aid615.tbin
617 1259 Southern Research Molecular Libraries Screening Center (SRMLSC) Southern Research Institute (Birmingham, Alabama) NIH Molecular Libraries Screening Centers Network (MLSCN) Assay Provider: Dr. Kim Lewis, Northeastern University Award: X01MH077622 There is considerable unmet need for novel antibiotics due to the rise of multi-drug resistant pathogens and the threat of engineered bioweapons. There is also an urgent need for novel antibiotics that can act against slow-growing or dormant pathogens a... aid617.table aid617.tbin
618 86750 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Number: XO1 MH78949-01 Assay Provider: Dr. Alex Strongin, Sanford-Burnham Medical Research Institute The sustained presence of matrix metalloproteinases (MMPs) in a tumor environment is a characteristic of many cancer types. The expression of the MT1-MMP mRNA and the MT1-MM... aid618.table aid618.tbin
619 97109 The PLK1 HTS assay was developed and run at the University of Pittsburgh Molecular Screening Center (PMLSC) as part of the Molecular Library Screening Center Network (MLSCN)(1 X01 MH76330). Polo-like kinase 1 (Plk1) is a serine-threonine protein kinase that functions as a key regulator of mitosis/meiosis and cytokinesis (Barr et al., 2004). Numerous research studies have demonstrated that Plk1 gene expression is frequently up regulated in human cancers and carcinoma-derived cell lines (Simiz... aid619.table aid619.tbin
620 86758 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Number: XO1 MH78949-01 Assay Provider: Dr. Alex Strongin, Sanford-Burnham Medical Research Institute This assay was developed to determine the cytotoxic effects of small molecule compounds on HT1080 fibrosarcoma cells that have been stably transfected with the luciferase ge... aid620.table aid620.tbin
621 185 Sanford-Burnham Center for Chemical Genomics (SBCCG) Sanford-Burnham Medical Research Institute (San Diego, CA) NIH Molecular Libraries Screening Centers Network (MLSCN) Bfl-1, also known as A1 in mice is an anti-apoptotic and NF-kB-inducible member of the Bcl-2 protein family involved in regulation of apoptosis. Due to difficulties with accomplishing targeted gene ablation in mouse models, the endogenous functions of Bfl-1 are largely unknown. Chemical inhibitors of Bfl-1 can be used as researc... aid621.table aid621.tbin
622 12369 Assay Provider: Ming Zhou Assay Provider Affiliation: Columbia University Grant Title: Small-molecule modulators of a family of voltage-dependent potassium channel Grant Number: 1 R03 MH076402-1 Voltage-dependent potassium channels (Kv) are integral membrane proteins that catalyze potassium ion diffusion across the cell membrane in response to voltage changes. Kv channels regulate membrane excitability and are essential to processes such as beating of the heart, communicating between neurons an... aid622.table aid622.tbin
623 12369 Assay Provider: Ming Zhou Assay Provider Affiliation: Columbia University Grant Title: Small-molecule modulators of a family of voltage-dependent potassium channel Grant Number: 1 R03 MH076402-1 Voltage-dependent potassium channels (Kv) are integral membrane proteins that catalyze potassium ion diffusion across the cell mmbrane in response to voltage changes. Kv channels regulate membrane excitability and are essential to processes such as beating of the heart, communicating between neurons and ... aid623.table aid623.tbin
624 8536 Assay Provider: Colleen Niswender Assay Provider Affliation: Vanderbilt University Grant Title: Measurement of GPCR-mediated thallium flux through GIRK channels Grant Number: 1 R03 MH076398-01 The aim of this work was to use high throughput screening of a small molecule library to identify compounds that interact with the alpha2C adrenergic receptor. The assay utilized thallium influx through G-protein Inwardly Rectifying K+ (GIRK) channels as a measure of alpha2C activation. Compounds were test... aid624.table aid624.tbin
625 12369 Assay Provider: Colleen Niswender Assay Provider Affliation: Vanderbilt University Grant Title: Discovery of Novel Allosteric Agonists of the M4 Muscarinic Receptor Grant Number: 1 X01 MH077607-1 The focus of this screening campaign was to identify highly selective small molecules that act as allosteric agonists of the M4 muscarinic receptor. The assay utilized thallium influx through G-protein Inwardly Rectifying K+ (GIRK) channels as a measure of M4 activation. Compounds were tested at 10uM fi... aid625.table aid625.tbin
626 63682 Assay Provider: P. Jeffery Conn Assay Provider Affiliation: Vanderbilt University Grant Title: Discovery of novel allosteric modulators of the M1 muscarinic receptor Grant Number: 1 R03 MH077606-01 The M1 muscarinic receptor is thought to be an important therapeutic target in schizophrenia. A cell-based fluorometric calcium assay was developed for high throughput screening. This assay was used to identify compounds with high selectivity for the M1 receptor subtype that act at an allosteric site ... aid626.table aid626.tbin
627 5 Assay Provider: Michelle Lewis Assay Provider Affiliation: Vanderbilt University Grant Title: Vanderbilt Screen Center - GPCRS, Ion Channels and Transporters Grant Number: 5U54MH074427-02 This assay measures the viability of HEK293 adherent cells using the cytoplasmic fluorescent indicator c-12-resorufin after 48hrs of exposure to test compounds. aid627.table aid627.tbin
628 63662 Assay Provider: P. Jeffrey Conn Assay Provider Affiliation: Vanderbilt University Grant Title: Discovery of novel allosteric modulators of the M1 muscarinic receptor Grant Number: 1 R03 MH077606-01 The M1 muscarinic receptor is thought to be an important therapeutic target in schizophrenia. A cell-based fluorometric calcium assay was developed for high throughput screening. This assay was used to identify compounds with high selectivity for the M1 receptor subtype that act at an allosteric site ... aid628.table aid628.tbin
629 86106 NIH Molecular Libraries Screening Centers Network [MLSCN] Emory Chemical Biology Discovery Center in MLSCN Assay provider: John A. Katzenellenbogen, University of Illinois at Urbana-Champaign MLSCN Grant: 1 X01MH78953-01 Title: HTS for Estrogen Receptor-alpha Coactivator Binding inhibitors Assay Overview Estrogens, which are responsible for the growth of many breast cancers, act through the estrogen receptors, ER-alpha and ER-beta, which are ligand-modulated transcription factors and memb... aid629.table aid629.tbin
630 88074 NIH Molecular Libraries Screening Centers Network [MLSCN] Emory Chemical Biology Discovery Center in MLSCN Assay provider: F.M. Hoffmann, University of Wisconsin-Madison MLSCN Grant: 1R21NS057002-01 Assay Overview: Transforming growth factor beta (TGF-Beta) regulates a variety of processes in mammalian cells, including proliferation, apoptosis, cell migration and extracellular matrix production. Aberrant increases in TGF-Beta signaling have been implicated in several pathological conditions ... aid630.table aid630.tbin
631 196256 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute, TSRI Assay Provider: Scripps Florida Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 1 X01 MH079861-01, Patrick Griffin, PI External Assay ID: PPARgSRC1_AG_HTRF_1536_%ACT Name: Primary biochemical High Throughput Screening assay for agonists of the steroid receptor coactivator 1 (SRC-1) recruitment by the peroxisome proli... aid631.table aid631.tbin
632 47 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] MLSCN Grant: X01 MH077625-01 NCGC Assay Overview: Hsp90 (heat shock protein 90) is the essential molecular chaperone and it accounts for 1-2% of all cytosolic proteins and is critical for the activity of diverse cellular proteins that are involved in a variety of cellular processes, including development, cell cycle, and steroid hormone signaling. Its client proteins include signaling kinases such as IG... aid632.table aid632.tbin
633 86106 NIH Molecular Libraries Screening Centers Network [MLSCN] Emory Chemical Biology Discovery Center in MLSCN Assay provider: John A. Katzenellenbogen, University of Illinois Urbana-Champaign MLSCN Grant: 1 X01MH78953-01 Assay Overview Estrogens, which are responsible for the growth of many breast cancers, act through the estrogen receptors, ER-alpha and ER-beta, which are ligand-modulated transcription factors and members of the nuclear receptor gene superfamily. ER-alpha and ER-beta are wel... aid633.table aid633.tbin
634 22 Assay Provider: Ming Zhou Assay Provider Affiliation: Columbia University Grant Title: Small-molecule modulators of a family of voltage-dependent potassium channel Grant Number: 1 R03 MH076402-1 Voltage-dependent potassium channels (Kv) are integral membrane proteins that catalyze potassium ion diffusion across the cell membrane in response to voltage changes. Kv channels regulate membrane excitability and are essential to processes such as beating of the heart, communicating between neurons and... aid634.table aid634.tbin
635 1265 Southern Research Molecular Libraries Screening Center (SRMLSC) Southern Research Institute (Birmingham, Alabama) NIH Molecular Libraries Screening Centers Network (MLSCN) Assay Provider: Dr. Kim Lewis, Northeastern University (Boston, MA) Award: X01MH077622 There is considerable unmet need for novel antibiotics due to the rise of multi-drug resistant pathogens and the threat of engineered bioweapons. There is also an urgent need for novel antibiotics that can act against slow-growing or dorman... aid635.table aid635.tbin
636 9808 Southern Research Molecular Libraries Screening Center (SRMLSC) Southern Research Institute (Birmingham, Alabama) NIH Molecular Libraries Screening Centers Network (MLSCN) Assay Provider: Dr. Edina Harsay, University of Kansas Award: X01-MH077628-01 Chemical Genetic Screens to Identify Modulators of Post-Golgi Transport The intracellular transport and targeted delivery of cell surface and secreted proteins is a fundamental process in all eukaryotic cells. The regulation of membrane transport pa... aid636.table aid636.tbin
637 9808 Southern Research Molecular Libraries Screening Center (SRMLSC) Southern Research Institute (Birmingham, Alabama) NIH Molecular Libraries Screening Centers Network (MLSCN) Assay Provider: Dr. Edina Harsay, University of Kansas Award: X01-MH077628-01 Chemical Genetic Screens to Identify Modulators of Post-Golgi Transport The intracellular transport and targeted delivery of cell surface and secreted proteins is a fundamental process in all eukaryotic cells. The regulation of membrane transport pa... aid637.table aid637.tbin
638 1265 Southern Research Molecular Libraries Screening Center (SRMLSC) Southern Research Institute (Birmingham, Alabama) NIH Molecular Libraries Screening Centers Network (MLSCN) Assay Provider: Dr. Kim Lewis, Northeastern University Award: X01MH077622 There is considerable unmet need for novel antibiotics due to the rise of multi-drug resistant pathogens and the threat of engineered bioweapons. There is also an urgent need for novel antibiotics that can act against slow-growing or dormant pathogens a... aid638.table aid638.tbin
639 86106 NIH Molecular Libraries Screening Centers Network [MLSCN] Emory Chemical Biology Discovery Center in MLSCN Assay provider: John A. Katzenellenbogen, University of Illinois Urbana-Champaign MLSCN Grant: 1 X01MH78953-01 Assay Overview Menopause is associated with the onset of hot flashes, night sweats, mood changes, and urogenital atrophy, which many women find distressing enough to seek medical management for relief. Estrogens in the form of hormone therapy (HT) have been the standard treatmen... aid639.table aid639.tbin
640 96409 LYP, a lymphocyte specific protein tyrosine phosphatase that plays a critical regulatory role in T cell receptor signaling, is encoded by the PTPN22 gene. A single-nucleotide polymorphism in PTPN22 is associated with a number of autoimmune disorders, including type 1 diabetes, rheumatoid arthritis, juvenile rheumatoid arthritis, systemic lupus erythematosus and Graves disease. The autoimmunity-predisposing allele is a gain-of-function mutant suggesting that its effect could be eliminated by a spe... aid640.table aid640.tbin
641 57709 Assay Provider: Val Watts Assay Provider Affiliation: Purdue University Grant Title: Allosteric Modulators of D1 Receptors Grant Number: 1 X01 MH077619-01 Dopamine receptors have been classified into two large families, the D1-like and the D2-like (Neve et al., 2004). Members of the D1-like receptor family include D1 and D5 dopamine receptors. Activation of D1-like receptors stimulate Gs which in turn activates adenylate cyclase resulting in enhanced cyclic AMP accumulation (Neve et al., 200... aid641.table aid641.tbin
642 2239 Assay Provider: Val Watts Assay Provider Affiliation: Purdue University Grant Title: Allosteric Modulators of D1 Receptors Grant Number: 1 X01 MH077619-01 Dopamine receptors have been classified into two large families, the D1-like and the D2-like (Neve et al., 2004). Members of the D1-like receptor family include D1 and D5 dopamine receptors. Activation of D1-like receptors stimulate Gs which in turn activates adenylate cyclase resulting in enhanced cyclic AMP accumulation (Neve et al., 200... aid642.table aid642.tbin
643 48 Assay Provider: Colleen Niswender Assay Provider Affliation: Vanderbilt University Grant Title: Discovery of Novel Allosteric Agonists of the M4 Muscarinic Receptor Grant Number: 1 X01 MH077607-1 The focus of this screening campaign was to identify highly selective small molecules that act as allosteric agonists of the M4 muscarinic receptor. The assay utilized thallium influx through G-protein Inwardly Rectifying K+ (GIRK) channels as a measure of M4 activation. Compounds were tested at 10uM fi... aid643.table aid643.tbin
644 206 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute, TSRI Assay Provider: Scripps Florida Network: Molecular Library Screening Center Network (MLSCN) Proposal Number: None External Assay ID: Rock2_INH_ LUMI_1536_ IC50 Name: Dose-response biochemical assay of inhibitors of Rho kinase 2 (Rock2) Description: Rho-Kinase is a serine/threonine kinase involved in the regulation of smooth muscle contraction and cyt... aid644.table aid644.tbin
645 64653 Southern Research Molecular Libraries Screening Center (SRMLSC), a member of the Molecular Libraries Screening Center Network (MLSCN) (Proposal number 1R03MH076408-012) Submitted by Dr. Gabriela Chiosis of the Memorial Sloan-Kettering Institute for Cancer Research Her2 (ErbB2) protein is over-expressed in breast and other solid tumors and is often mutated in cancer patients with progressive disease. Her2 is a member of a family of four transmembrane tyrosine kinase receptors. It can form heter... aid645.table aid645.tbin
646 1909 Assay Provider: Val Watts Assay Provider Affiliation: Purdue University Grant Title: Allosteric Modulators of D1 Receptors Grant Number: 1 X01 MH077619-01 Dopamine receptors have been classified into two large families, the D1-like and the D2-like (Neve et al., 2004). Members of the D1-like receptor family include D1 and D5 dopamine receptors. Activation of D1-like receptors stimulate Gs which in turn activates adenylate cyclase resulting in enhanced cyclic AMP accumulation (Neve et al., 2004). ... aid646.table aid646.tbin
647 2240 Assay Provider: Val Watts Assay Provider Affiliation: Purdue University Grant Title: Allosteric Modulators of D1 Receptors Grant Number: 1 X01 MH077619-01 Dopamine receptors have been classified into two large families, the D1-like and the D2-like (Neve et al., 2004). Members of the D1-like receptor family include D1 and D5 dopamine receptors. Activation of D1-like receptors stimulate Gs which in turn activates adenylate cyclase resulting in enhanced cyclic AMP accumulation (Neve et al., 2004). ... aid647.table aid647.tbin
648 86170 Southern Research Molecular Libraries Screening Center (SRMLSC) Southern Research Institute (Birmingham, Alabama) NIH Molecular Libraries Screening Centers Network (MLSCN) Assay Provider: Dr. Zhican Qu, Southern Research Institute Award: X01MH079851-01 Angiogenesis is a process of new blood vessel formation. Endothelial cell proliferation is an essential step during the angiogenesis process and is involved in many human diseases including cancer, diabetic retinopathy, and rheumatoid arthritis. ... aid648.table aid648.tbin
649 29 The Cdc25B Phosphatase probe assessment dose response assay in 25 mM DTT has been developed to assess actives that were identified in the Cdc25B HTS AID 368 and confirmed in the Cdc25B 10-pt dose response confirmation assay AID 569 conducted by the University of Pittsburgh Molecular Screening Center (PMLSC) part of the Molecular Library Screening Center Network (MLSCN). XO1 submission MH078959 Cdc25B catalytic domain in vitro assay, Assay Provider Dr. Marni Brisson, Department of Pharmacology at ... aid649.table aid649.tbin
650 29 The Cdc25B Phosphatase probe assessment dose response reproducibility assay has been developed to re-confirm actives that were identified in the Cdc25B HTS AID 368 and confirmed in the Cdc25B 10-pt dose response confirmation assay AID 569 conducted by the University of Pittsburgh Molecular Screening Center (PMLSC) part of the Molecular Library Screening Center Network (MLSCN). XO1 submission MH078959 Cdc25B catalytic domain in vitro assay, Assay Provider Dr. Marni Brisson, Department of Pharmaco... aid650.table aid650.tbin
651 1003 The HIV-1 RT-RNase H assay was submitted by Dr. Michael Parniak of the University of Pittsburgh, MLSCN XO1 MH077605, and the HTS was developed and screened at the University of Pittsburgh Molecular Library Screening Center (PMLSC), AID 565. In order to confirm actives identified in the HIV RNase H primary HTS, a cherry pick order of compounds that produced >/= 50% inhibition was re-screened at 10 uM in duplicate wells. The rapid development of HIV-1 resistance to antiretroviral agents is a major... aid651.table aid651.tbin
652 390 The HIV-1 RT-RNase H assay was submitted by Dr. Michael Parniak of the University of Pittsburgh, MLSCN XO1 MH077605, and the HTS was developed and screened at the University of Pittsburgh Molecular Library Screening Center (PMLSC), AID 565. Actives identified in the HIV RNase H primary HTS, were confirmed by re-screening at 10 uM in duplicate wells, AID (pending). If the compound was active in the primary HTS, and the % inhibition in both of the confirmation duplicate tests was > 50%, it was foll... aid652.table aid652.tbin
653 126 The HTS assay to identify Inhibitors of West Nile Virus (WNV) NS2bNS3 Proteinase was proposed by Dr Alex Strongin of the Burnham Institute XO1-MH077601, and was developed and screened at the University of Pittsburgh Molecular Library Screening Center part of the Molecular Library Screening Center Network (MLSCN). The 10-point IC50 dose response confirmation assay was developed and run at the PMLSC to confirm the activity of WNV NS2bNS3 Proteinase inhibitors identified in the primary HTS, AID 577.... aid653.table aid653.tbin
654 1408 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] National Institutes of Environmental Health Sciences [NIEHS] National Toxicology Program [NTP] Grant number: None NCGC Assay Overview: We have developed a 1536-well cell-based assay for quantitative high throughput screening (qHTS) against a number of cell lines to determine in vitro caspase 3/7 of small molecules. This particular assay uses the HepG2 cell line which is derived from human hepatocellula... aid654.table aid654.tbin
655 1408 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] National Institutes of Environmental Health Sciences [NIEHS] National Toxicology Program [NTP] Grant number: None NCGC Assay Overview: We have developed a 1536-well cell-based assay for quantitative high throughput screening (qHTS) against a number of cell lines to determine in vitro caspase 3/7 of small molecules. This particular assay uses the Jurkat cell line which is derived from human T cell leuke... aid655.table aid655.tbin
656 1408 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] National Institutes of Environmental Health Sciences [NIEHS] National Toxicology Program [NTP] Grant number: None NCGC Assay Overview: We have developed a 1536-well cell-based assay for quantitative high throughput screening (qHTS) against a number of cell lines to determine in vitro caspase 3/7 of small molecules. This particular assay uses the HUV-EC-C cell line which is derived from normal human vas... aid656.table aid656.tbin
657 1408 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] National Institutes of Environmental Health Sciences [NIEHS] National Toxicology Program [NTP] Grant number: None NCGC Assay Overview: We have developed a 1536-well cell-based assay for quantitative high throughput screening (qHTS) against a number of cell lines to determine in vitro caspase 3/7 activity of small molecules. This particular assay uses the SH-SY5Y cell line which is derived from human ne... aid657.table aid657.tbin
658 1408 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] National Institutes of Environmental Health Sciences [NIEHS] National Toxicology Program [NTP] Grant number: None NCGC Assay Overview: We have developed a 1536-well cell-based assay for quantitative high throughput screening (qHTS) against a number of cell lines to determine in vitro caspase 3/7 activity of small molecules. This particular assay uses BJ cell line which is derived from normal human fore... aid658.table aid658.tbin
659 1408 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] National Institutes of Environmental Health Sciences [NIEHS] National Toxicology Program [NTP] Grant number: None NCGC Assay Overview: We have developed a 1536-well cell-based assay for quantitative high throughput screening (qHTS) against a number of cell lines to determine in vitro caspase 3/7 activity of small molecules. This particular assay uses the MRC-5 cell line which is derived from human norm... aid659.table aid659.tbin
660 1408 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] National Institutes of Environmental Health Sciences [NIEHS] National Toxicology Program [NTP] Grant number: None NCGC Assay Overview: We have developed a 1536-well cell-based assay for quantitative high throughput screening (qHTS) against a number of cell lines to determine in vitro caspase 3/7 of small molecules. This particular assay uses the mesangial cell line which is derived from normal human re... aid660.table aid660.tbin
661 1408 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] National Institutes of Environmental Health Sciences [NIEHS] National Toxicology Program [NTP] Grant number: None NCGC Assay Overview: We have developed a 1536-well cell-based assay for quantitative high throughput screening (qHTS) against a number of cell lines to determine in vitro caspase 3/7 activity of small molecules. This particular assay uses the SK-N-SH cell line which is derived from human ne... aid661.table aid661.tbin
662 70701 NIH Molecular Libraries Screening Centers Network [MLSCN] NIH Chemical Genomics Center [NCGC] MLSCN Grant: 1X01MH079867-01 Assay Provider: Marshall W. Nirenberg, U.S. National heart lung & blood institute NCGC Assay Overview Memories persist for different lengths of time, from seconds or minutes to a lifetime. There are two kinds of memory: short-term memory (STM) and long-term memory (LTM). STM lasts for minutes to hours, which maybe mediated by modifications of molecules involved in synaptic... aid662.table aid662.tbin
663 1408 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] National Institutes of Environmental Health Sciences [NIEHS] National Toxicology Program [NTP] Grant number: None NCGC Assay Overview: We have developed a 1536-well cell-based assay for quantitative high throughput screening (qHTS) against a number of cell lines to determine in vitro caspase 3/7 of small molecules. This particular assay uses the H-4-II-E cell line which is derived from rat hepatoma. aid663.table aid663.tbin
664 1408 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] National Institutes of Environmental Health Sciences [NIEHS] National Toxicology Program [NTP] Grant number: None NCGC Assay Overview: We have developed a 1536-well cell-based assay for quantitative high throughput screening (qHTS) against a number of cell lines to determine in vitro caspase 3/7 of small molecules. This particular assay uses the Hek 293 cell line which is derived from human embryonic k... aid664.table aid664.tbin
665 1408 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] National Institutes of Environmental Health Sciences [NIEHS] National Toxicology Program [NTP] Grant number: None NCGC Assay Overview: We have developed a 1536-well cell-based assay for quantitative high throughput screening (qHTS) against a number of cell lines to determine in vitro caspase 3/7 activity of small molecules. This particular assay uses the N2a cell line which is derived from mouse neurob... aid665.table aid665.tbin
666 1408 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] National Institutes of Environmental Health Sciences [NIEHS] National Toxicology Program [NTP] Grant number: None NCGC Assay Overview: We have developed a 1536-well cell-based assay for quantitative high throughput screening (qHTS) against a number of cell lines to determine in vitro caspase 3/7 of small molecules. This particular assay uses the NIH 3T3 cell line which is derived from mouse fibroblasts... aid666.table aid666.tbin
667 1408 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] National Institutes of Environmental Health Sciences [NIEHS] National Toxicology Program [NTP] Grant number: None NCGC Assay Overview: We have developed a 1536-well cell-based assay for quantitative high throughput screening (qHTS) against a number of cell lines to determine in vitro caspase 3/7 activity of small molecules. This particular assay uses the primary kidney proximal tubule cells freshly iso... aid667.table aid667.tbin
668 29 The Cdc25B Phosphatase probe assessment dose response assay in Catalase has been developed to assess actives that were identified in the Cdc25B HTS AID 368 and confirmed in the Cdc25B 10-pt dose response confirmation assay AID 569 conducted by the University of Pittsburgh Molecular Screening Center (PMLSC) part of the Molecular Library Screening Center Network (MLSCN). XO1 submission MH078959 Cdc25B catalytic domain in vitro assay, Assay Provider Dr. Marni Brisson, Department of Pharmacology at t... aid668.table aid668.tbin
669 29 The Cdc25B Phosphatase probe assessment dose response assay in 25 mM DTT with Catalase has been developed to assess actives that were identified in the Cdc25B HTS AID 368 and confirmed in the Cdc25B 10-pt dose response confirmation assay AID 569 conducted by the University of Pittsburgh Molecular Screening Center (PMLSC) part of the Molecular Library Screening Center Network (MLSCN). XO1 submission MH078959 Cdc25B catalytic domain in vitro assay, Assay Provider Dr. Marni Brisson, Department of Ph... aid669.table aid669.tbin
670 29 The Cdc25B Phosphatase probe assessment dose response assay in Beta-Mercaptoethanol (BME) has been developed to assess actives that were identified in the Cdc25B HTS AID 368 and confirmed in the Cdc25B 10-pt dose response confirmation assay AID 569 conducted by the University of Pittsburgh Molecular Screening Center (PMLSC) part of the Molecular Library Screening Center Network (MLSCN). XO1 submission MH078959 Cdc25B catalytic domain in vitro assay, Assay Provider Dr. Marni Brisson, Department of... aid670.table aid670.tbin
671 29 The Cdc25B Phosphatase probe assessment dose response assay in Glutathione (GSH) has been developed to assess actives that were identified in the Cdc25B HTS AID 368 and confirmed in the Cdc25B 10-pt dose response confirmation assay AID 569 conducted by the University of Pittsburgh Molecular Screening Center (PMLSC) part of the Molecular Library Screening Center Network (MLSCN). XO1 submission MH078959 Cdc25B catalytic domain in vitro assay, Assay Provider Dr. Marni Brisson, Department of Pharmaco... aid671.table aid671.tbin
672 58 Hydrogen peroxide (H2O2) can modulate (activate or inhibit) the activity of a variety of proteins including protein kinases, protein phosphatases, transcription factors, phospholipases, ion channels and G proteins. H2O2 is capable of oxidizing the cysteine residues of proteins that may be crucial for their catalytic and/or structural function. Many proteins, including a significant number of the targets screened by the MLSCN, contain an active site cysteine that is required for biological activi... aid672.table aid672.tbin
673 29 The Cdc25B Phosphatase probe assessment MKP-1 dose response selectivity assay has been developed to test the phosphatase specificity of actives that were identified in the Cdc25B HTS AID 368 and confirmed in the Cdc25B 10-pt dose response confirmation assay AID 569 conducted by the University of Pittsburgh Molecular Screening Center (PMLSC) part of the Molecular Library Screening Center Network (MLSCN). XO1 submission MH078959 Cdc25B catalytic domain in vitro assay, Assay Provider Dr. Marni Bris... aid673.table aid673.tbin
674 29 The Cdc25B Phosphatase probe assessment MKP-1 dose response selectivity assay has been developed to test the phosphatase specificity of actives that were identified in the Cdc25B HTS AID 368 and confirmed in the Cdc25B 10-pt dose response confirmation assay AID 569 conducted by the University of Pittsburgh Molecular Screening Center (PMLSC) part of the Molecular Library Screening Center Network (MLSCN). XO1 submission MH078959 Cdc25B catalytic domain in vitro assay, Assay Provider Dr. Marni Bris... aid674.table aid674.tbin
675 107 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute, TSRI Assay Provider: Orphagen Pharmaceuticals, San Diego, CA Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 1X01-MH077624-01 External Assay ID: RORA_AG_LUMI_1536_CS_EC50 Name: Counterscreen for activators of the nuclear receptor Steroidogenic Factor 1 (SF-1): A cell-based dose-response assay for inhibition of the RAR-rela... aid675.table aid675.tbin
676 46 The MKP-1 Dual Specificity Protein Tyrosine Phosphatase Probe Assessment Cdc25B Dose Response Selectivity Assay has been developed to evaluate the PTP selectivity of actives identified in the MH-76391 In vitro HTS assay for MKP-1 inhibitors. The MKP-1 HTS assay was screened by the PMLSC against the year 1 full diversity NIH compound library (65,239 compounds) and the data were uploaded to the PubChem database AID 374. The PMLSC confirmed the actives from the MKP-1 screen in dose response assays a... aid676.table aid676.tbin
677 1665 Assay Provider: P. Jeffrey Conn Assay Provider Affiliation: Vanderbilt University Grant Title: Discovery of novel allosteric modulators of the M1 muscarinic receptor Grant Number: 1 R03 MH077606-01 The M1 muscarinic receptor is thought to be an important therapeutic target in schizophrenia. A cell-based fluorometric calcium assay was developed for high throughput screening. This assay was used to identify compounds with high selectivity for the M1 receptor subtype that act at an allosteric site ... aid677.table aid677.tbin
678 1665 Assay Provider: P. Jeffrey Conn Assay Provider Affiliation: Vanderbilt University Grant Title: Discovery of novel allosteric modulators of the M1 muscarinic receptor Grant Number: 1 R03 MH077606-01 The M1 muscarinic receptor is thought to be an important therapeutic target in schizophrenia. A cell-based fluorometric calcium assay was developed for high throughput screening. This assay was used to identify compounds with high selectivity for the M1 receptor subtype that act at an allosteric site ... aid678.table aid678.tbin
679 94 Molecular Library Screening Center Network (MLSCN) Penn Center for Molecular Discovery (PCMD) Assay Provider: Scott L. Diamond, University of Pennsylvania MLSCN Grant: X01-MH076406-01 Target Factor XI (FXI) circulates as a complex with high molecular weight kininogen (HK) in the plasma at a concentration of 5 ug/ml (equivalent to 31.3 nM, dimeric concentration) as a homodimeric glycosylated blood plasma zymogen of approximately 160 kDa, containing monomeric subunits of 80 kDa each (1). Throm... aid679.table aid679.tbin
680 62106 Molecular Library Screening Center Network (MLSCN) Penn Center for Molecular Discovery (PCMD) Assay Provider: Scott L. Diamond, University of Pennsylvania MLSCN Grant: X01-MH076406-01 Target Factor XI (FXI) circulates as a complex with high molecular weight kininogen (HK) in the plasma at a concentration of 5 ug/ml (equivalent to 31.3 nM, dimeric concentration) as a homodimeric glycosylated blood plasma zymogen of approximately 160 kDa, containing monomeric subunits of 80 kDa each (1). Throm... aid680.table aid680.tbin
681 63 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute, TSRI Assay Provider: Orphagen Pharmaceuticals, San Diego, CA Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 1X01-MH077624-01 External Assay ID: RORA_AG_LUMI_1536_EC50 Name: Dose-response cell-based assay for activators of the Retinoic Acid Receptor-related orphan receptor A (RORA) Description: Nuclear receptors are a fam... aid681.table aid681.tbin
682 29 The Cdc25B Phosphatase probe assessment compound dose dependent redox cycling H2O2 generation assay in the presence of 0.5 mM DTT, has been developed to evaluate actives that were identified in the Cdc25B HTS AID 368 and confirmed in the Cdc25B 10-pt dose response confirmation assay AID 569 conducted by the University of Pittsburgh Molecular Screening Center (PMLSC) part of the Molecular Library Screening Center Network (MLSCN). XO1 submission MH078959 Cdc25B catalytic domain in vitro assay, Assa... aid682.table aid682.tbin
683 29 The Cdc25B Phosphatase probe assessment compound dose dependent redox cycling H2O2 generation assay in the presence of 1.0 mM DTT, has been developed to evaluate actives that were identified in the Cdc25B HTS AID 368 and confirmed in the Cdc25B 10-pt dose response confirmation assay AID 569 conducted by the University of Pittsburgh Molecular Screening Center (PMLSC) part of the Molecular Library Screening Center Network (MLSCN). XO1 submission MH078959 Cdc25B catalytic domain in vitro assay, Assa... aid683.table aid683.tbin
684 62104 Target Factor XII (FXII) is a 80 kDa zymogen found at a concentration of 0.375 uM in plasma, and upon activation by kallikrein at R353, a disulfide-linked two chain molecule called factor XIIa alpha (FXIIa) is generated. FXIIa is also capable of autoactivation by binding to negatively charged surfaces (1). Kallikrein can also cleave other scissile bonds in FXIIa alpha outside of the catalytic domain at R334, R343, and R353, generating FXIIa beta, a 30 kDa enzyme that is no longer able to bind ... aid684.table aid684.tbin
685 30077 Molecular Library Screening Center Network (MLSCN) Penn Center for Molecular Discovery (PCMD) Assay Provider: Dr. Graham Pavitt, University of Manchester, U.K. MLSCN Grant: X01-MH077608-01 eIF2B-related disorders are caused by genetically inherited mutations in the general translation initiation factor eIF2B [Pavitt GD, Ramaiah KV, Kimball SR, Hinnebusch AG, Genes Dev. 1998, 12, 514-26]. We are currently screening the MLSCN library to identify compounds that can restore eIF2B function using wild... aid685.table aid685.tbin
686 3806 Molecular Library Screening Center Network (MLSCN) Penn Center for Molecular Discovery (PCMD) Assay Provider: Dr. Amy Rubenstein, Zygogen LLC, Atlanta, GA MLSCN Grant: X01-MH077634-01 The zebrafish processes lipids through its digestive system in a similar way to mammals. Thus it is a useful model organism that provides for in vivo measurement of lipid absorption and processing in a vertebrate organism. Zebrafish larvae are transparent, allowing for observation of lipid metabolism in the whole ... aid686.table aid686.tbin
687 33069 Molecular Library Screening Center Network (MLSCN) Penn Center for Molecular Discovery (PCMD) Assay Provider: Scott L. Diamond, University of Pennsylvania MLSCN Grant: X01-MH076406-01 Target Factor XI (FXI) circulates as a complex with high molecular weight kininogen (HK) in the plasma at a concentration of 5 ug/ml (equivalent to 31.3 nM, dimeric concentration) as a homodimeric glycosylated blood plasma zymogen of approximately 160 kDa, containing monomeric subunits of 80 kDa each (1). Throm... aid687.table aid687.tbin
688 27198 Molecular Library Screening Center Network (MLSCN) Penn Center for Molecular Discovery (PCMD) Assay Provider: Dr. Graham Pavitt, University of Manchester, U.K. MLSCN Grant: X01-MH077608-01 eIF2B is a translation initiation factor that functions in the first step of protein synthesis. It is a guanine-nucleotide exchange factor (GEF), converting eIF2 (inactive GDP-bound form) to eIF2GTP (active) [Pavitt GD, Ramaiah KV, Kimball SR, Hinnebusch AG, Genes Dev. 1998, 12, 514-26.] Mutations of eIF2B man... aid688.table aid688.tbin
689 61808 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Number: XO1 MH077603-01 Assay Provider: Dr. Elena Pasquale, Sanford-Burnham Medical Research Institute EphA4 is a member of the large Eph family of receptor tyrosine kinases. The signaling ability of EphA4, and the other nine closely related EphA receptors, is activated by ... aid689.table aid689.tbin
690 95864 Sanford-Burnham Center for Chemical Genomics (SBCCG) Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) NIH Molecular Libraries Screening Centers Network (MLSCN) Alkaline phosphatase (EC 3.1.3.1) (APs) catalyze the hydrolysis of phosphomonoesters, releasing phosphate and alcohol. APs are dimeric enzymes found in most organisms. In human, four isozymes of APs have been identified: three isozymes are tissue-specific and the fourth one is tissue-nonspecific. Placental alkaline phosph... aid690.table aid690.tbin
691 53 Molecular Library Screening Center Network (MLSCN) Penn Center for Molecular Discovery (PCMD) Assay Provider: Dr. Amy Rubenstein, Zygogen LLC, Atlanta, GA MLSCN Grant: X01-MH077634-01 The zebrafish processes lipids through its digestive system in a similar way to mammals. Thus it is a useful model organism that provides for in vivo measurement of lipid absorption and processing in a vertebrate organism. Zebrafish larvae are transparent, allowing for observation of lipid metabolism in the whole ... aid691.table aid691.tbin
692 107 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute, TSRI Assay Provider: Orphagen Pharmaceuticals, San Diego, CA Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 1X01-MH077624-01 External Assay ID: SF1_AG_LUMI_1536_EC50 Nuclear receptors are a family of small molecule and hormone-regulated transcription factors that share conserved DNA-binding and ligand-binding domains. Small... aid692.table aid692.tbin
693 97049 Extracted from the MH078944 proposal submitted by Dr. Michael Yaffe of MIT. The Polo-like kinases (Plks) play important roles in many cell cycle-related events including the initiation of mitosis, chromosome segregation, centrosome maturation, bipolar spindle formation, regulation of anaphase-promoting complex and execution of cytokinesis. The prototypic Polo kinase was originally identified in flies, in which mutants resulted in abnormal spindle poles. A single Polo family member is found in fl... aid693.table aid693.tbin
694 1280 NIH Molecular Libraries Screening Centers Network [MLSCN] Emory Chemical Biology Discovery Center in MLSCN Assay provider: John A. Katzenellenbogen, University of Illinois at Urbana-Champaign MLSCN Grant: 1 X01MH78953-01 Assay Overview Estrogens, which are responsible for the growth of many breast cancers, act through the estrogen receptors, ER-alpha and ER-beta, which are ligand-modulated transcription factors and members of the nuclear receptor gene superfamily. ER-alpha and ER-alpha are... aid694.table aid694.tbin
695 63 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute, TSRI Assay Provider: Orphagen Pharmaceuticals, San Diego, CA (1-X01-MH077624-01) Network: Molecular Library Screening Center Network (MLSCN) External Assay ID: SF1_AG_LUMI_1536_CS_EC50 Name: Counterscreen for activators of the Retinoic Acid Receptor-related orphan receptor A (RORA): A cell-based dose-response assay for inhibition of the Steroidogenic Factor ... aid695.table aid695.tbin
696 95739 Sanford-Burnham Center for Chemical Genomics (SBCCG) Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) NIH Molecular Libraries Screening Centers Network (MLSCN) Alkaline phosphatase (EC 3.1.3.1) (APs) catalyze the hydrolysis of phosphomonoesters, releasing phosphate and alcohol. APs are dimeric enzymes found in most organisms. In human, four isozymes of APs have been identified: three isozymes are tissue-specific and the fourth one is tissue-nonspecific. Placental alkaline phosph... aid696.table aid696.tbin
697 96409 LYP, a lymphocyte specific protein tyrosine phosphatase that plays a critical regulatory role in T cell receptor signaling, is encoded by the PTPN22 gene. A single-nucleotide polymorphism in PTPN22 is associated with a number of autoimmune disorders, including type 1 diabetes, rheumatoid arthritis, juvenile rheumatoid arthritis, systemic lupus erythematosus and Graves disease. The autoimmunity-predisposing allele is a gain-of-function mutant, suggesting that an animal model could be created with ... aid697.table aid697.tbin
698 7 University of New Mexico Assay Overview: Assay Support NIH 1R03MH076381-01 Assay for Formylpeptide Receptor Family Ligands PI: Bruce S. Edwards, Ph.D. Assay Background and Significance Formyl peptide receptors. The G-protein coupled formylpeptide receptor (FPR) was one of the originating members of the chemoattractant receptor superfamily (Le et al., 2002a; Oppenheim et al., 1991). N-formylated peptides such as fMLF are high affinity FPR ligands that trigger a variety of biologic activities i... aid698.table aid698.tbin
699 34 University of New Mexico Assay Overview: Assay Support NIH 1R03MH076381-01 Assay for Formylpeptide Receptor Family Ligands PI: Bruce S. Edwards, Ph.D. Assay Background and Significance Formyl peptide receptors. The G-protein coupled formylpeptide receptor (FPR) was one of the originating members of the chemoattractant receptor superfamily (Le et al., 2002a; Oppenheim et al., 1991). N-formylated peptides such as fMLF are high affinity FPR ligands that trigger a variety of biologic activities ... aid699.table aid699.tbin
700 94 University of New Mexico Assay Overview Assay Support 1X01MH078952-01 Small Molecule Inhibition of Staphylococcus aureus Virulence PI: Hattie D. Gresham, Ph.D. Background and Significance Quorum sensing is a cell-to-cell communication system that permits members of a bacterial population to coordinate their behavior dependent on cell density (for review see ref. Waters and Bassler, 2005). The mediators of this communication system are small, diffusible pheromones or autoinducers that are se... aid700.table aid700.tbin
701 33069 Molecular Library Screening Centre Network (MLSCN) Penn Center for Molecular Discovery (PCMD) Assay Provider: Scott L. Diamond, University of Pennsylvania MLSCN Grant: X01-MH076406-01 Target Factor XII (FXII) is a 80 kDa zymogen found at a concentration of 0.375 uM in plasma, and upon activation by kallikrein at R353, a disulfide-linked two chain molecule called factor XIIa alpha (FXIIa) is generated. FXIIa is also capable of autoactivation by binding to negatively charged surfaces (1). K... aid701.table aid701.tbin
702 62 Assay Support: 1 X01 MH077638-01 MLSCN Assay for Allosteric Ligands for the VLA-4 Integrin PI: SKLAR, LARRY A University of New Mexico Assay Overview: This is a high throughput flow cytometry cell-based assay to measure dose response of small molecules# allosteric inhibition of integrin alpha-4-beta-1 heterodimer very late antigen (VLA-4). Inhibition of VLA-4 activation affects the affinity and conformation of integrins. Affinity states have been directly evaluated by a peptide ligand derived ... aid702.table aid702.tbin
703 5 Assay Support: 1 X01 MH077638-01 MLSCN Assay for Allosteric Ligands for the VLA-4 Integrin PI: SKLAR, LARRY A University of New Mexico Assay Overview: This is a high throughput flow cytometry cell-based assay to measure dose response of small molecules allosteric activators of integrin alpha-4-beta-1 heterodimer very late antigen (VLA-4). Activation of VLA-4 affects the affinity and conformation of integrins. Affinity states have been directly evaluated by a peptide ligand derived from the LD... aid703.table aid703.tbin
704 96889 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute, TSRI Assay Provider: The Scripps Research Institute, TSRI Network: Molecular Library Screening Center Network (MLSCN) Proposal Number: 1 X01 MH078935-01 External Assay ID: HIVREVRRE_INH_FRET_1536_%INH Name: Primary biochemical high-throughput screening assay for inhibitors of the HIV Rev - RRE RNA interaction (disruption of protein-RNA interaction) Descri... aid704.table aid704.tbin
705 9532 Southern Research Molecular Libraries Screening Center (SRMLSC) Southern Research Institute (Birmingham, Alabama) NIH Molecular Libraries Screening Centers Network (MLSCN) Assay Provider: Dr. David S. Goldfarb, University of Rochester Award: R03 MH076395-01 There is now solid evidence for the early evolution of conserved pathways for aging. These pathways may allow eukaryotic cells and animals to postpone reproduction in unfavorable environmental conditions. Key elements of public cellular mecha... aid705.table aid705.tbin
706 9532 Southern Research Molecular Libraries Screening Center (SRMLSC) Southern Research Institute (Birmingham, Alabama) NIH Molecular Libraries Screening Centers Network (MLSCN) Assay Provider: Dr. David S. Goldfarb, University of Rochester Award: R03 MH076395-01 There is now solid evidence for the early evolution of conserved pathways for aging. These pathways may allow eukaryotic cells and animals to postpone reproduction in unfavorable environmental conditions. Key elements of public cellular mecha... aid706.table aid706.tbin
707 90649 Hypertension and vascular inflammation are associated with cardiovascular diseases, the primary cause of death in our society. Because a large proportion of patients are not responding to current therapies, the next generation of drugs will not only need to reduce blood pressure but also treat vascular and renal inflammation as well as reduce smooth muscle cell proliferation, which in turn should also reduce hypertension related organ damage. Using inhibitors developed in the Hammock laboratory, ... aid707.table aid707.tbin
708 65448 Southern Research Molecular Libraries Screening Center (SRMLSC) Southern Research Institute (Birmingham, Alabama) NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Number: None When small molecule libraries are screened in bioassays, a common source of artifacts and interference is various optical properties of the compounds themselves. If an assay is using absorbance at a certain wavelength as a readout, it is possible that some screened compounds will absorb light efficiently en... aid708.table aid708.tbin
709 65445 Southern Research Molecular Libraries Screening Center (SRMLSC) Southern Research Institute (Birmingham, Alabama) NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Number: None When small molecule libraries are screened in bioassays, a common source of artifacts and interference is various optical properties of the compounds themselves. Autofluorescent compounds may for example yield false positives or negatives in screens where an increase or decrease of fluorescence is measured o... aid709.table aid709.tbin
710 97559 Southern Research Molecular Libraries Screening Center (SRMLSC) Southern Research Institute (Birmingham, Alabama) NIH Molecular Libraries Screening Centers Network (MLSCN) Assay Provider: Dr. Kim Lewis, Northeastern University Award: X01MH077622 There is considerable unmet need for novel antibiotics due to the rise of multi-drug resistant pathogens and the threat of engineered bioweapons. There is also an urgent need for novel antibiotics that can act against slow-growing or dormant pathogens a... aid710.table aid710.tbin
711 52 NIH Molecular Libraries Screening Centers Network [MLSCN] Emory Chemical Biology Discovery Center in MLSCN MLSCN Grant: 1 X01MH78953-01 The 14-3-3 proteins are the prototype for a novel class of protein modules that can recognize phosphoserine/threonine (pS/T)-containing motifs in a variety of signaling proteins. To date, 14-3-3 proteins have been reported to bind more than 200 client proteins. Through these interactions, 14-3-3 proteins play important roles in a wide range of vital regulatory p... aid711.table aid711.tbin
712 268 Emory Chemistry-Biology Discovery Center Assay Overview: MLSCN Grant: 1 X01MH78953-01 Hsp90 is a chaperon with important roles in maintaining transformation and in elevating the survival and growth potential of cancer cells. Recent evidence suggests additional applications of Hsp90 inhibitors in neurodegenerative diseases, nerve injuries, inflammation and infection. Several natural products that inactivate Hsp90 function have anti-tumor effects in in vitro and in vivo models of cancer. Howe... aid712.table aid712.tbin
713 439 NIH Molecular Libraries Screening Centers Network [MLSCN] Emory Chemical Biology Discovery Center in MLSCN Assay provider: John A. Katzenellenbogen, University of Illinois at Urbana-Champaign MLSCN Grant: 1 X01MH78953-01 Title: HTS for Estrogen Receptor-alpha Coactivator Binding inhibitors Assay Overview Estrogens, which are responsible for the growth of many breast cancers, act through the estrogen receptors, ER-alpha and ER-beta, which are ligand-modulated transcription factors and memb... aid713.table aid713.tbin
714 42 NIH Molecular Libraries Screening Centers Network [MLSCN] Emory Chemical Biology Discovery Center in MLSCN Assay provider: Keith D. Wilkinson, Emory University MLSCN Grant: 1 R03 MH076382-01 Assay Overview: BAP1 (BRCA1 associated protein 1) is a member of the Ubiquitin carboxy-terminal hydrolase (UCH) family of deubiquitinating enzymes(DUB). These proteases reverse the conjugation of ubiquitin to targeted proteins. The importance of ubiquitin conjugation in many cellular processes suggests... aid714.table aid714.tbin
715 30 NIH Molecular Libraries Screening Centers Network [MLSCN] Emory Chemical Biology Discovery Center in MLSCN Assay provider: F.M. Hoffmann, University of Wisconsin-Madison MLSCN Grant: 1R21NS057002-01 Assay Overview: Transforming growth factor beta (TGF-Beta) regulates a variety of processes in mammalian cells, including proliferation, apoptosis, cell migration and extracellular matrix production. Aberrant increases in TGF-Beta signaling have been implicated in several pathological conditions ... aid715.table aid715.tbin
716 93 Molecular Library Screening Center Network (MLSCN) Penn Center for Molecular Discovery (PCMD) Assay Provider: Scott L. Diamond, University of Pennsylvania MLSCN Grant: X01-MH076406-01 Target Factor XII (FXII) is a 80 kDa zymogen found at a concentration of 0.375 uM in plasma, and upon activation by kallikrein at R353, a disulfide-linked two chain molecule called factor XIIa alpha (FXIIa) is generated. FXIIa is also capable of autoactivation by binding to negatively charged surfaces (1). K... aid716.table aid716.tbin
717 90649 Hypertension and vascular inflammation are associated with cardiovascular diseases, the primary cause of death in our society. Because a large proportion of patients are not responding to current therapies, the next generation of drugs will not only need to reduce blood pressure but also treat vascular and renal inflammation as well as reduce smooth muscle cell proliferation, which in turn should also reduce hypertension related organ damage. Using inhibitors developed in the Hammock laboratory, ... aid717.table aid717.tbin
718 51 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute, TSRI Assay Provider: The Scripps Research Institute Molecular Screening Center Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 1 R03 MH076345-01 Assay Description: Widely expressed in the human brain, 5-hydroxytryptamine (5-HT, serotonin) receptors have been shown to have an important role in depression as well as other cogni... aid718.table aid718.tbin
719 84890 Southern Research Molecular Libraries Screening Center (SRMLSC) Southern Research Institute (Birmingham, Alabama) NIH Molecular Libraries Screening Centers Network (MLSCN) Assay Provider: Dr. Zhican Qu, Southern Research Institute Award: X01-MH077628-01 Assay Description Angiogenesis is the process of new blood vessel formation, which is believed to be involved in many human diseases including cancer, diabetic retinopathy, and rheumatoid arthritis. Endothelial cell proliferation is known to oc... aid719.table aid719.tbin
720 96889 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute, TSRI Assay Provider: The Scripps Research Institute, TSRI Network: Molecular Library Screening Center Network (MLSCN) Proposal Number: 1 X01 MH079857-01 External Assay ID: EphB4TNYLRAW_INH_FP_1536_%INH Name: Primary biochemical high-throughput screening assay for antagonists of the interaction between the Eph receptor B4 (EphB4) and its ligand ephrin-B2 via ... aid720.table aid720.tbin
721 94 Molecular Library Screening Center Network (MLSCN) Penn Center for Molecular Discovery (PCMD) Assay Provider: Scott L. Diamond, University of Pennsylvania MLSCN Grant: X01-MH076406-01 Target Factor XI (FXI) circulates as a complex with high molecular weight kininogen (HK) in the plasma at a concentration of 5 ug/ml (equivalent to 31.3 nM, dimeric concentration) as a homodimeric glycosylated blood plasma zymogen of approximately 160 kDa, containing monomeric subunits of 80 kDa each (1). Throm... aid721.table aid721.tbin
722 1276 University of New Mexico Assay Overview: Assay Support: NIH 1R03MH076381-01 Assay for Formylpeptide Receptor Family Ligands PI: Bruce S. Edwards, Ph.D. Assay Background and Significance Formyl peptide receptors. The G-protein coupled formylpeptide receptor (FPR) was one of the originating members of the chemoattractant receptor superfamily (Le et al., 2002a; Oppenheim et al., 1991). N-formylated peptides such as fMLF are high affinity FPR ligands that trigger a variety of biologic activitie... aid722.table aid722.tbin
723 58 University of New Mexico Assay Overview: Assay Support: NIH 1R03MH076381-01 Assay for Formylpeptide Receptor Family Ligands PI: Bruce S. Edwards, Ph.D. Assay Background and Significance Formyl peptide receptors. The G-protein coupled formylpeptide receptor (FPR) was one of the originating members of the chemoattractant receptor superfamily (Le et al., 2002a; Oppenheim et al., 1991). N-formylated peptides such as fMLF are high affinity FPR ligands that trigger a variety of biologic activiti... aid723.table aid723.tbin
724 58 University of New Mexico Assay Overview: Assay Support: NIH 1R03MH076381-01 Assay for Formylpeptide Receptor Family Ligands PI: Bruce S. Edwards, Ph.D. Assay Background and Significance Formyl peptide receptors. The G-protein coupled formylpeptide receptor (FPR) was one of the originating members of the chemoattractant receptor superfamily (Le et al., 2002a; Oppenheim et al., 1991). N-formylated peptides such as fMLF are high affinity FPR ligands that trigger a variety of biologic activiti... aid724.table aid724.tbin
725 1276 University of New Mexico Assay Overview: Assay Support: NIH 1R03MH076381-01 Assay for Formylpeptide Receptor Family Ligands PI: Bruce S. Edwards, Ph.D. Assay Background and Significance Formyl peptide receptors. The G-protein coupled formylpeptide receptor (FPR) was one of the originating members of the chemoattractant receptor superfamily (Le et al., 2002a; Oppenheim et al., 1991). N-formylated peptides such as fMLF are high affinity FPR ligands that trigger a variety of biologic activitie... aid725.table aid725.tbin
726 51 Data Source: The Scripps Research Institute Molecular Screening Center Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute, TSRI Assay Provider: The Scripps Research Institute Molecular Screening Center Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 1 R03 MH076345-01 Description The neurotransmitter, serotonin (5HT, 5-hydroxytryptamine) is important in a large number of neuro... aid726.table aid726.tbin
727 96889 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute, TSRI Assay Provider: Scripps Florida Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: Not Applicable External Assay ID: FAK_INH_TRFRET_1536_%INH Name: Primary biochemical high-throughput screening assay for inhibitors of Focal Adhesion Kinase (FAK) Description: The focal adhesion kinase (FAK) is a tyrosine kinase involve... aid727.table aid727.tbin
728 87 Molecular Library Screening Center Network (MLSCN) Penn Center for Molecular Discovery (PCMD) Assay Provider: Scott L. Diamond, University of Pennsylvania MLSCN Grant: X01-MH076406-01 Target Factor XII (FXII) is a 80 kDa zymogen found at a concentration of 0.375 uM in plasma, and upon activation by kallikrein at R353, a disulfide-linked two chain molecule called factor XIIa alpha (FXIIa) is generated. FXIIa is also capable of autoactivation by binding to negatively charged surfaces (1). K... aid728.table aid728.tbin
729 96889 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute, TSRI Assay Provider: TSRI Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 1 R03 MH076533-01 External Assay ID: S1P2_AG_BLA_1536_%ACT Name: Primary Cell-Based High-Throughput Screening to Identify Agonists of the Sphingosine 1-phosphate receptor 2 (S1P2) Description: Sphingosine 1-phosphate (S1P) influences heart rate [1,2... aid729.table aid729.tbin
730 51 External Assay ID: S1P3_EC50_CS_5HT1e_Agonist Name: S1P3 Dose Response Assay Counterscreen for 5-Hydroxytryptamine(Serotonin) Receptor Subtype 1E Agonists Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute, TSRI Assay Provider: The Scripps Research Institute Molecular Screening Center Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 1 R03 MH076345-01 Description: The neurotr... aid730.table aid730.tbin
731 196256 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute, TSRI Assay Provider: Scripps Florida Network: Molecular Library Screening Center Network (MLSCN) Proposal Number: 1 X01 MH079861-01 PI: Patrick Griffin External Assay ID: PPARgSRC3_AG_TRFRET_1536_%ACT Name: Primary biochemical High Throughput Screening assay for agonists of the steroid receptor coactivator 3 (SRC-3) recruitment by the peroxisome proliferator-... aid731.table aid731.tbin
732 1307 NIH Molecular Libraries Screening Centers Network [MLSCN] Emory Chemical Biology Discovery Center in MLSCN Assay provider: Dr. Thanh Doan & Eric Sandberg, ZYGOGEN, LLC MLSCN Grant: 1 X01 MH077629-01 Assay Overview: Pathological angiogenesis contributes to over 70 diseases, including cancer, age-related macular degeneration and rheumatoid arthritis. Current in vitro models employed in screening compounds for effects on angiogenesis lack the biological complexity of in vivo systems. Zygogen, LLC d... aid732.table aid732.tbin
733 390 NIH Molecular Libraries Screening Centers Network [MLSCN] Emory Chemical Biology Discovery Center in MLSCN Assay provider: John A. Katzenellenbogen, University of Illinois Urbana-Champaign MLSCN Grant: 1 X01MH78953-01 Assay Overview Estrogens, which are responsible for the growth of many breast cancers, act through the estrogen receptors, ER-alpha and ER-beta, which are ligand-modulated transcription factors and members of the nuclear receptor gene superfamily. ER-alpha and ER-beta are wel... aid733.table aid733.tbin
734 5149 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute, TSRI Assay Provider: Florida Atlantic University Network: Molecular Library Screening Center Network (MLSCN) Proposal Number: 1 X01 MH078948-01 External Assay ID: MMP13_INH_deltaRFU_ 1536_3X%INH Name: Assay to identify inhibitors among the possible fluorescent artifacts from the primary HTS inhibition assay of Matrix Metalloproteinase 13 (MMP13) activity Des... aid734.table aid734.tbin
735 42 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute, TSRI Assay Provider: Florida Atlantic University Network: Molecular Library Screening Center Network (MLSCN) Proposal Number: 1 X01 MH078948-01 External Assay ID: MMP13_INH_fTHP_1536_IC50 Name: Dose-response biochemical assay for inhibitors of Matrix Metalloproteinase 13 (MMP13) activity Description: Osteoarthritis (OA) is an age-related debilitating diseas... aid735.table aid735.tbin
736 96889 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute, TSRI Assay Provider: TSRI Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 1 R03 MH076533-01 External Assay ID: S1P2_ANT_BLA_1536_%INH Name: Primary Cell-Based High-Throughput Screening to Identify Antagonists of the Sphingosine 1-phosphate receptor 2 (S1P2) Description: Sphingosine 1-phosphate (S1P) influences heart rate [... aid736.table aid736.tbin
737 96 NIH Molecular Libraries Screening Centers Network [MLSCN] Emory Chemical Biology Discovery Center in MLSCN Assay provider: John A. Katzenellenbogen, University of Illinois Urbana-Champaign MLSCN Grant: 1 X01MH78953-01 Assay Overview Menopause is associated with the onset of hot flashes, night sweats, mood changes, and urogenital atrophy, which many women find distressing enough to seek medical management for relief. Estrogens in the form of hormone therapy (HT) have been the standard treatmen... aid737.table aid737.tbin
738 97528 Southern Research Molecular Libraries Screening Center (SRMLSC) Southern Research Institute (Birmingham, Alabama) NIH Molecular Libraries Screening Centers Network (MLSCN) Assay Provider: Dr. Edina Harsay, University of Kansas Award: X01-MH077628-01 Chemical Genetic Screens to Identify Modulators of Post-Golgi Transport The intracellular transport and targeted delivery of cell surface and secreted proteins is a fundamental process in all eukaryotic cells. The regulation of membrane transport pa... aid738.table aid738.tbin
739 97519 Southern Research Molecular Libraries Screening Center (SRMLSC) Southern Research Institute (Birmingham, Alabama) NIH Molecular Libraries Screening Centers Network (MLSCN) Assay Provider: Dr. Edina Harsay, University of Kansas Award: X01-MH077628-01 Chemical Genetic Screens to Identify Modulators of Post-Golgi Transport The intracellular transport and targeted delivery of cell surface and secreted proteins is a fundamental process in all eukaryotic cells. The regulation of membrane transport pa... aid739.table aid739.tbin
740 95520 Southern Research Molecular Libraries Screening Center (SRMLSC) Southern Research Institute (Birmingham, Alabama) NIH Molecular Libraries Screening Centers Network (MLSCN) Assay Provider: Dr. William E. Severson, Southern Research Institute Award: R03 MH081270-01 Currently, there is no commercially available vaccine to protect humans against the highly pathogenic avian influenza H5N1 virus that is spreading across Asia, Europe, and Africa. Since humans have no immunity against any H5 viruses, th... aid740.table aid740.tbin
741 296 Southern Research Molecular Libraries Screening Center (SRMLSC) Southern Research Institute (Birmingham, Alabama) NIH Molecular Libraries Screening Centers Network (MLSCN) (Proposal number 1X01-MH077620-01) Submitted by Dr. Gary A. Piazza of Southern Research Institute Drug resistance, whether intrinsic or acquired, is a major clinical obstacle, which limits the efficacy of cancer chemotherapy. Multi-drug resistance (MDR) is a phenomenon by which tumor cells display or develop resistance to a n... aid741.table aid741.tbin
742 300 Southern Research Molecular Libraries Screening Center (SRMLSC) Southern Research Institute (Birmingham, Alabama) NIH Molecular Libraries Screening Centers Network (MLSCN) Assay Provider: Dr. Gabriela Chiosis, Memorial Sloan-Kettering Cancer Center Award: 1R03MH076408-012 Her2 (ErbB2) protein is over-expressed in breast and other solid tumors and is often mutated in patients with progressive disease. Her2 is a member of a family of four transmembrane tyrosine kinase receptors. It can form hetero... aid742.table aid742.tbin
744 209 The PLK1 HTS assay was developed and run at the University of Pittsburgh Molecular Screening Center (PMLSC) as part of the Molecular Library Screening Center Network (MLSCN)(1 X01 MH76330). Polo-like kinase 1 (Plk1) is a serine-threonine protein kinase that functions as a key regulator of mitosis/meiosis and cytokinesis (Barr et al., 2004). Numerous research studies have demonstrated that Plk1 gene expression is frequently up regulated in human cancers and carcinoma-derived cell lines (Simizu a... aid744.table aid744.tbin
745 33360 Emory Chemistry-Biology Discovery Center Assay Overview: Grant number: none Paramyxoviruses comprise several major human pathogens. Although a live-attenuated vaccine protects against measles virus (MV), a member of the paramyxovirus family, the virus remains a principal cause of worldwide mortality and accounts for approximately 21 million cases and 300,000 to 400,000 deaths annually. The development of novel antivirals that allow improved case management of severe measles and silence viral out... aid745.table aid745.tbin
746 59805 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute, TSRI Assay Provider: Scripps Florida Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: None External Assay ID: JNK3_INH_TR-FRET_1536_%INH Name: Primary biochemical high-throughput screening assay for inhibitors of the c-Jun N-Terminal Kinase 3 (JNK3) Description: The c-Jun N-Terminal Kinases (JNK) are members of the mitogen a... aid746.table aid746.tbin
747 26 Differential static light scattering assay using StarGazer instrument from Harbinger Biotech: Protein samples are heated gradually, with light scattered by aggregated protein recorded as a function of temperature. aid747.table aid747.tbin
748 96416 Sanford-Burnham Center for Chemical Genomics (SBCCG) Sanford-Burnham Medical Research Institute (San Diego, CA) NIH Molecular Libraries Screening Centers Network (MLSCN) Bcl-B is an anti-apoptotic member of the Bcl-2 family that is prominently expressed in plasma and multiple myeloma cells. TR3 (NR4A1; HMR; NP10; GFRP1; NAK1; NUR77; NGFIB) is an orphan member of the steroid/thyroid/retinoid nuclear receptor superfamily that translocates from cellular nuclei to mitochondria upon exposure to vario... aid748.table aid748.tbin
749 44 External Assay ID: Dose Response Cell Based Assay for Antagonists of the 5-Hydroxytryptamine Receptor Subtype 1E (5HT1E) Name: 5H1E_Antagonists_IC50 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute, TSRI Assay Provider: The Scripps Research Institute Molecular Screening Center Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 1 R03 MH076345-01 Description: The neurotransmi... aid749.table aid749.tbin
750 86480 Sanford-Burnham Center for Chemical Genomics (SBCCG) Sanford-Burnham Medical Research Institute (San Diego, CA) NIH Molecular Libraries Screening Centers Network (MLSCN) The sustained presence of matrix metalloproteinases (MMPs) in a tumor environment is a characteristic of many cancer types. The expression of the MT1-MMP mRNA and the MT1-MMP protein closely correlates with increased tumor volume, tumor invasiveness, and the incidence of local and distant metastases. Tumorigenic MT1-MMP is effe... aid750.table aid750.tbin
751 23718 University of New Mexico Assay Overview: Assay Support: NIH 1 X01 MH077613-01 Assay for disassembly of the 26S Proteasome PI: Dorota Skowyra, PhD Assay implementation: Peter Simons PhD, Irena Ivnitski-Steele PhD, Terry Foutz BS, Mark Carter MS, Anna Waller PhD Assay Background and Significance The 26S proteasome in a highly conserved ATP-dependent protease that plays a central role in the control of protein stability in all eukaryotic cells. It is the most complex example of ATP-dependent p... aid751.table aid751.tbin
752 2 Organism: Rattus norvegicus; Strain: Wistar. RNAi silencing of endogenous KLF5 and Edg1 expression was performed on primary culture of vascular smooth muscle cells (VSMCs). aid752.table aid752.tbin
753 309 NIH Molecular Libraries Screening Centers Network [MLSCN] Emory Chemical Biology Discovery Center in MLSCN Grant number: 1 R03 MH076382-01 BAP1 (BRCA1 Associated Protein 1) is a member of the Ubiquitin carboxy-terminal hydrolase (UCH) family of deubiquitinating enzymes (DUB). These proteases reverse the conjugation of ubiquitin to targeted proteins. The importance of ubiquitin conjugation in many cellular processes suggests a critical role of DUBs in normal physiology and potentially in patholog... aid753.table aid753.tbin
754 81 Emory Chemistry-Biology Discovery Center Assay Overview: MLSCN Grant: 1 X01MH78953-01 Hsp90 is a chaperon with important roles in maintaining transformation and in elevating the survival and growth potential of cancer cells. Recent evidence suggests additional applications of Hsp90 inhibitors in neurodegenerative diseases, nerve injuries, inflammation and infection. Several natural products that inactivate Hsp90 function have anti-tumor effects in in vitro and in vivo models of cancer. Howe... aid754.table aid754.tbin
755 44 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute, TSRI Assay Provider: The Scripps Research Institute Molecular Screening Center Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 1 R03 MH076345-01 Assay Description: Widely expressed in the human brain, 5-hydroxytryptamine (5-HT, serotonin) receptors have been shown to have an important role in depression as well as other cogni... aid755.table aid755.tbin
756 209 The Plk1 HTS assay was developed and run at the University of Pittsburgh Molecular Screening Center (PMLSC) as part of the Molecular Library Screening Center Network (MLSCN)(1 X01 MH76330). Polo-like kinase 1 (Plk1) is a serine-threonine protein kinase that functions as a key regulator of mitosis/meiosis and cytokinesis (Barr et al., 2004). Numerous research studies have demonstrated that Plk1 gene expression is frequently up regulated in human cancers and carcinoma-derived cell lines (Simiz... aid756.table aid756.tbin
757 194655 University of New Mexico Assay Overview: Assay Support: NIH I RO3 MH081231-01 HTS to identify specific small molecule inhibitors of Ras and Ras-related GTPases PI: Angela Wandinger-Ness, Ph.D. Co-PI: Larry Sklar, Ph.D. Assay Development: Zurab Surviladze, Ph.D. Assay Implementation: Zurab Surviladze, Danuta Wlodek, Terry Foutz, Mark Carter, Anna Waller Target Team Leader for the Center: Larry Sklar (lsklar@salud.unm.edu) Assay Background and Significance: Ras and related small molecular weight... aid757.table aid757.tbin
758 194659 University of New Mexico Assay Overview: Assay Support: NIH I RO3 MH081231-01 HTS to identify specific small molecule inhibitors of Ras and Ras-related GTPases PI: Angela Wandinger-Ness, Ph.D. Co-PI: Larry Sklar, Ph.D. Assay Development: Zurab Surviladze, Ph.D. Assay Implementation: Zurab Surviladze, Danuta Wlodek, Terry Foutz, Mark Carter, Anna Waller Target Team Leader for the Center: Larry Sklar (lsklar@salud.unm.edu) Assay Background and Significance: Ras and related small molecular weight... aid758.table aid758.tbin
759 194653 University of New Mexico Assay Overview: Assay Support: NIH I RO3 MH081231-01 HTS to identify specific small molecule inhibitors of Ras and Ras-related GTPases PI: Angela Wandinger-Ness, Ph.D. Co-PI: Larry Sklar, Ph.D. Assay Development: Zurab Surviladze, Ph.D. Assay Implementation: Zurab Surviladze, Danuta Wlodek, Terry Foutz, Mark Carter, Anna Waller Target Team Leader for the Center: Larry Sklar (lsklar@salud.unm.edu) Assay Background and Significance: Ras and related small molecular weight... aid759.table aid759.tbin
760 194656 University of New Mexico Assay Overview: Assay Support: NIH I RO3 MH081231-01 HTS to identify specific small molecule inhibitors of Ras and Ras-related GTPases PI: Angela Wandinger-Ness, Ph.D. Co-PI: Larry Sklar, Ph.D. Assay Development: Zurab Surviladze, Ph.D. Assay Implementation: Zurab Surviladze, Danuta Wlodek, Terry Foutz, Mark Carter, Anna Waller Target Team Leader for the Center: Larry Sklar (lsklar@salud.unm.edu) Assay Background and Significance: Ras and related small molecular weight... aid760.table aid760.tbin
761 194656 University of New Mexico Assay Overview: Assay Support: NIH I RO3 MH081231-01 HTS to identify specific small molecule inhibitors of Ras and Ras-related GTPases PI: Angela Wandinger-Ness, Ph.D. Co-PI: Larry Sklar, Ph.D. Assay Development: Zurab Surviladze, Ph.D. Assay Implementation: Zurab Surviladze, Danuta Wlodek, Terry Foutz, Mark Carter, Anna Waller Target Team Leader for the Center: Larry Sklar (lsklar@salud.unm.edu) Assay Background and Significance: Ras and related small molecular weight... aid761.table aid761.tbin
762 15 University of New Mexico Assay Overview: Assay Support: NIH 1R03MH076381-01 Assay for Formylpeptide Receptor Family Ligands PI: Bruce S. Edwards, Ph.D. Assay Background and Significance Formyl peptide receptors. The G-protein coupled formylpeptide receptor (FPR) was one of the originating members of the chemoattractant receptor superfamily (Le et al., 2002a; Oppenheim et al., 1991). N-formylated peptides such as fMLF are high affinity FPR ligands that trigger a variety of biologic activities... aid762.table aid762.tbin
763 170 University of New Mexico Assay Overview: Assay Support: NIH 1R03MH076381-01 Assay for Formylpeptide Receptor Family Ligands PI: Bruce S. Edwards, Ph.D. Assay Background and Significance Formyl peptide receptors. The G-protein coupled formylpeptide receptor (FPR) was one of the originating members of the chemoattractant receptor superfamily (Le et al., 2002a; Oppenheim et al., 1991). N-formylated peptides such as fMLF are high affinity FPR ligands that trigger a variety of biologic activities ... aid763.table aid763.tbin
764 194653 University of New Mexico Assay Overview: Assay Support: NIH I RO3 MH081231-01 HTS to identify specific small molecule inhibitors of Ras and Ras-related GTPases PI: Angela Wandinger-Ness, Ph.D. Co-PI: Larry Sklar, Ph.D. Assay Development: Zurab Surviladze, Ph.D. Assay Implementation: Zurab Surviladze, Danuta Wlodek, Terry Foutz, Mark Carter, Anna Waller Target Team Leader for the Center: Larry Sklar (lsklar@salud.unm.edu) Assay Background and Significance: Ras and related small molecular weight... aid764.table aid764.tbin
765 170 University of New Mexico Assay Overview: Assay Support: NIH 1R03MH076381-01 Assay for Formylpeptide Receptor Family Ligands PI: Bruce S. Edwards, Ph.D. Assay Background and Significance Formyl peptide receptors. The G-protein coupled formylpeptide receptor (FPR) was one of the originating members of the chemoattractant receptor superfamily (Le et al., 2002a; Oppenheim et al., 1991). N-formylated peptides such as fMLF are high affinity FPR ligands that trigger a variety of biologic activities ... aid765.table aid765.tbin
766 15 University of New Mexico Assay Overview: Assay Support: NIH 1R03MH076381-01 Assay for Formylpeptide Receptor Family Ligands PI: Bruce S. Edwards, Ph.D. Assay Background and Significance Formyl peptide receptors. The G-protein coupled formylpeptide receptor (FPR) was one of the originating members of the chemoattractant receptor superfamily (Le et al., 2002a; Oppenheim et al., 1991). N-formylated peptides such as fMLF are high affinity FPR ligands that trigger a variety of biologic activities... aid766.table aid766.tbin
767 3 Organism: Homo sapiens. RNAi silencing of endogenous TRBP was performed on HeLa cells (cervical adenocarcinoma). aid767.table aid767.tbin
768 3 Organism: Homo sapiens. RNAi silencing of transfected TRBP was performed on HeLa cells (cervical adenocarcinoma). aid768.table aid768.tbin
769 8 External Assay ID: MMP13_INH_deltaRFU_ 1536_IC50 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute, TSRI Assay Provider: Florida Atlantic University Network: Molecular Library Screening Center Network (MLSCN) Proposal Number: 1 X01 MH078948-01 Name: Dose response biochemical assay for autofluorescent inhibitors of Matrix Metalloproteinase 13 (MMP13) activity Description: Osteoarthritis (OA) is an age-related ... aid769.table aid769.tbin
770 3316 Southern Research Molecular Libraries Screening Center (SRMLSC) Southern Research Institute (Birmingham, Alabama) NIH Molecular Libraries Screening Centers Network (MLSCN) Grant number: None Compounds that display in vitro tumor cell growth inhibitory activity may have antiproliferative, apoptotic, or non-selective cytotoxic effects and vary widely in structure and mechanism of action. Cancer chemotherapeutic drugs display this activity primarily by disrupting cell division, which often results... aid770.table aid770.tbin
771 3317 Southern Research Molecular Libraries Screening Center (SRMLSC) Southern Research Institute (Birmingham, Alabama) NIH Molecular Libraries Screening Centers Network (MLSCN) Grant number: None Compounds that display in vitro tumor cell growth inhibitory activity may have antiproliferative, apoptotic, or non-selective cytotoxic effects and vary widely in structure and mechanism of action. Cancer chemotherapeutic drugs display this activity primarily by disrupting cell division, which often results... aid771.table aid771.tbin
772 3317 Southern Research Molecular Libraries Screening Center (SRMLSC) Southern Research Institute (Birmingham, Alabama) NIH Molecular Libraries Screening Centers Network (MLSCN) Grant number: None Compounds that display in vitro tumor cell growth inhibitory activity may have antiproliferative, apoptotic, or non-selective cytotoxic effects and vary widely in structure and mechanism of action. Cancer chemotherapeutic drugs display this activity primarily by disrupting cell division, which often results ... aid772.table aid772.tbin
773 270 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Number: XO1 MH78949-01 Assay Provider: Dr. Alex Strongin, Sanford-Burnham Medical Research Institute This functional assay was developed for detection of compounds inhibiting luciferase. These compounds would be observed as false positives of assays employing luciferase-bas... aid773.table aid773.tbin
774 65449 Southern Research Molecular Libraries Screening Center (SRMLSC) Southern Research Institute (Birmingham, Alabama) NIH Molecular Libraries Screening Centers Network (MLSCN) Grant number: None A common readout in enzymatic assays is to use a coupled enzymatic reaction that in the end leads to the oxidation of NADH to NAD+ or vice versa. This results in an inexpensive readout where either the change in absorbance or fluorescence in the assay mixture is measured. Usually the last enzyme in the sequ... aid774.table aid774.tbin
775 132796 Southern Research Molecular Libraries Screening Center (SRMLSC) Southern Research Institute (Birmingham, Alabama) NIH Molecular Libraries Screening Centers Network (MLSCN) Assay Provider: Dr. David S. Goldfarb, University of Rochester Award: R03 MH076395-01 There is now solid evidence for the existence of conserved pathways that regulate cell aging and senescence. These pathways may have evolved to allow eukaryotic cells and animals to remain reproductively viable for long periods during unfavor... aid775.table aid775.tbin
776 44 External Assay ID: S1P3_IC50_CS_5H1E_Antagonists Name: S1P3 Dose Response Assay Counterscreen for 5-Hydroxytryptamine(Serotonin) Receptor Subtype 1E Antagonists Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute, TSRI Assay Provider: The Scripps Research Institute Molecular Screening Center Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 1 R03 MH076345-01 Description: The neur... aid776.table aid776.tbin
777 95868 Sanford-Burnham Center for Chemical Genomics (SBCCG) Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) NIH Molecular Libraries Screening Centers Network (MLSCN) The cytochrome P450 enzymes represent a diverse superfamily of hemoproteins present in eukaryotic, bacterial, and archaean systems. The primary function of these enzymes is in the metabolism and clearance of both endogenous and exogenous (xenobiotic) compounds due to their propensity to metabolize multiple substrates thro... aid777.table aid777.tbin
778 95867 Sanford-Burnham Center for Chemical Genomics (SBCCG) Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) NIH Molecular Libraries Screening Centers Network (MLSCN) The cytochrome P450 enzymes represent a diverse superfamily of hemoproteins present in eukaryotic, bacterial, and archaean systems. The primary function of these enzymes is in the metabolism and clearance of both endogenous and exogenous (xenobiotic) compounds due to their propensity to metabolize multiple substrates thro... aid778.table aid778.tbin
779 134 Sanford-Burnham Center for Chemical Genomics (SBCCG) Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Number: MH077609-01 This functional assay was developed for detection of compounds inhibiting placental alkaline phosphatase. These compounds would be observed as false positives of assays employing alkaline phosphatase-based detection. This assay was primarily utilized as counter screen for EphA4 hits identified ... aid779.table aid779.tbin
780 36 Assay Provider: Colleen Niswender Assay Provider Affliation: Vanderbilt University Grant Title: Measurement of GPCR-mediated thallium flux through GIRK channels Grant Number: 1 R03 MH076398-01 The aim of this work was to use high throughput screening of a small molecule library to identify compounds that interact with the alpha2C adrenergic receptor. The assay utilized thallium influx through G-protein Inwardly Rectifying K+ (GIRK) channels as a measure of alpha2C activation. Compounds were test... aid780.table aid780.tbin
781 217516 Ultra-High Throughput Screening for 14-3-3/Bad interaction inhibitors NIH Molecular Libraries Screening Centers Network [MLSCN] Emory Chemical Biology Discovery Center in MLSCN Assay provider: Dr. Haian Fu, Emory University MLSCN Grant: 1R03MH76385-1 Assay Overview 14-3-3 proteins are a family of phosphoserine/threonine binding proteins, which consists of seven isoforms in mammalian cells (Fu et al, Ann Rev of Pharm & Tox 40:617-47; 2000). These isoforms are encoded by genes in different chro... aid781.table aid781.tbin
782 217516 Screening for Small Molecule Inhibitors of Eukaryotic Translation Initiation NIH Molecular Libraries Screening Centers Network [MLSCN] Emory Chemical Biology Discovery Center in MLSCN Assay provider: Dr. Jerry Pelletier, McGill UNIVERSITY MLSCN Grant: 1 R03 MH081216-01 Title: uHTS for Small Molecule Inhibitors of Eukaryotic Translation Initiation Assay Overview The recruitment of the 40S ribosomal subunit and associated factors (43S pre-initiation complex) to the mRNA during translation initia... aid782.table aid782.tbin
783 3 RNAi silencing of endogenous genes was performed on HeLa cells (cervical adenocarcinoma) by transfection of small interfering RNAs. aid783.table aid783.tbin
784 29549 External Assay ID: BetaGlucosidase_L444P_Primary_Screening Name: Primary Cell Based High Throughput Screening Assay for Enhancers of Beta-Glucosidase Activity Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute, TSRI Assay Provider: The Scripps Research Institute Molecular Screening Center Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: MH078940-01 Assay Overview: Gaucher dise... aid784.table aid784.tbin
785 40 The Plk1 HTS assay was developed and run at the University of Pittsburgh Molecular Screening Center (PMLSC) as part of the Molecular Library Screening Center Network (MLSCN)(1 X01 MH76330). Polo-like kinase 1 (Plk1) is a serine-threonine protein kinase that functions as a key regulator of mitosis/meiosis and cytokinesis (Barr et al., 2004). Numerous research studies have demonstrated that Plk1 gene expression is frequently up regulated in human cancers and carcinoma-derived cell lines (Simiz... aid785.table aid785.tbin
786 33 Sanford-Burnham Center for Chemical Genomics (SBCCG) Sanford-Burnham Medical Research Institute (San Diego, CA) NIH Molecular Libraries Screening Centers Network (MLSCN) MLSCN Grant: XO1 MH079863-01 Over-expression of molecular chaperones occurs commonly in cancers and provides protection from a wide variety of cellular stresses, both endogenous and iatrogenic. Molecular chaperones also play important roles in maintaining the activity of several signal-transducing proteins and transcriptions fac... aid786.table aid786.tbin
787 184 Molecular Library Screening Center Network (MLSCN) Penn Center for Molecular Discovery (PCMD) Assay Provider: Dr. Scott L. Diamond, University of Pennsylvania MLSCN Grant: X01-MH076406-01 Complement factor C1s (EC 3.4.21.42) is a trypsin-like serine protease that is activated in one of the first steps in the classical complement cascade. Despite the essential role for the complement cascade in immune defense, unregulated activation leading to acute inflammation and tissue damage has been implica... aid787.table aid787.tbin
788 320 Southern Research Molecular Libraries Screening Center (SRMLSC) Southern Research Institute (Birmingham, Alabama) NIH Molecular Libraries Screening Centers Network (MLSCN) Assay Provider: Dr. Edina Harsay, University of Kansas Award: X01-MH077628-01 Chemical Genetic Screens to Identify Modulators of Post-Golgi Transport The intracellular transport and targeted delivery of cell surface and secreted proteins is a fundamental process in all eukaryotic cells. The regulation of membrane transport pa... aid788.table aid788.tbin
789 320 Southern Research Molecular Libraries Screening Center (SRMLSC) Southern Research Institute (Birmingham, Alabama) NIH Molecular Libraries Screening Centers Network (MLSCN) Assay Provider: Dr. Edina Harsay, University of Kansas Award: X01-MH077628-01 Chemical Genetic Screens to Identify Modulators of Post-Golgi Transport The intracellular transport and targeted delivery of cell surface and secreted proteins is a fundamental process in all eukaryotic cells. The regulation of membrane transport pa... aid789.table aid789.tbin
790 320 Southern Research Molecular Libraries Screening Center (SRMLSC) Southern Research Institute (Birmingham, Alabama) NIH Molecular Libraries Screening Centers Network (MLSCN) Assay Provider: Dr. Edina Harsay, University of Kansas Award: X01-MH077628-01 Chemical Genetic Screens to Identify Modulators of Post-Golgi Transport The intracellular transport and targeted delivery of cell surface and secreted proteins is a fundamental process in all eukaryotic cells. The regulation of membrane transport pa... aid790.table aid790.tbin
791 23 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute, TSRI Assay Provider: Scripps Florida Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: None Name: Dose-response biochemical assay of Rho kinase 2 (Rock2) inhibitors Description: Rho-Kinase is a serine/threonine kinase involved in the regulation of smooth muscle contraction and cytoskeletal reorganization of nonmuscle cells (... aid791.table aid791.tbin
792 448 Molecular Library Screening Center Network (MLSCN) Penn Center for Molecular Discovery (PCMD), University of Pennsylvania Assay Provider: Dr. Graham Pavitt, University of Manchester, U.K. MLSCN Grant: X01-MH077608-01 eIF2B is a translation initiation factor that functions in the first step of protein synthesis. It is a guanine-nucleotide exchange factor (GEF), converting eIF2 (inactive GDP-bound form) to eIF2GTP (active). Mutations of eIF2B manifest as a dysfunction in brain myelin leading to i... aid792.table aid792.tbin
793 140109 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Affiliation: The Scripps Research Institute, TSRI Assay Provider: Scripps Florida Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number 1 R21 NS056950-01 (Fast Track) PI: Claes Wahlestedt External Assay ID: NPY-Y2_ANT_CNGC_1536_%INH Name: Primary cell based high-throughput screening assay for antagonists of neuropeptide Y receptor Y2 (NPY-Y2) Description: Neuropeptide Y (NPY) is a n... aid793.table aid793.tbin
794 753 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute, TSRI Assay Provider: Scripps Florida Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: Not Applicable PI: Peter Hodder External Assay ID: FAK_INH_TRFRET_1536_%INH_3X# Name: Confirmation biochemical assay for inhibitors of Focal Adhesion Kinase (FAK) Description: The focal adhesion kinase (FAK) is a tyrosine kinase involved in g... aid794.table aid794.tbin
795 23718 University of New Mexico Assay Overview Assay Support: 1 X01 MH078937-01 MLSCN Assay for Activators of Prostate Cell Differentiation PI: Todd A. Thompson, PhD Assay Implementation: Mark Haynes PhD, Mark Carter MS Assay Background and Significance: Prostate cancer is the third leading cause of cancer-related deaths among men in the United States [Jemal A, et al. 2005]. Although there is no known etiology, the majority of cases are adenocarcinomas that develop from glandular epithelium by sub... aid795.table aid795.tbin
796 94275 Inflammatory disease requires that endothelial cells detect and amplify a pro-inflammatory signal. This results in the adherence, activation, and ultimately transmigration of lymphocytes at the site of damage (Pober, 2002). Drugs that block this process would significantly alleviate the symptoms of inflammation. Our assay detects an early event in this process, the nuclear translocation of the transcription factor, NFkappaB (Senftleben, et al., 2002). Chronic inflammatory disease is believed to p... aid796.table aid796.tbin
797 196173 The PKD HTS assay was developed and run at the University of Pittsburgh Molecular Screening Center (PMLSC) as part of the Molecular Library Screening Center Network (MLSCN)(1R03DA24898-01). Protein kinase D (PKD) is a novel family of serine/threonine kinases targeted by diacylglycerol. It regulates many fundamental cell functions including cell proliferation, survival, differentiation and protein trafficking, and plays important roles in pathological conditions such as cardiac hypertrophy and c... aid797.table aid797.tbin
798 218784 Molecular Library Screening Center Network (MLSCN) Penn Center for Molecular Discovery (PCMD) Assay Provider: Scott L. Diamond, University of Pennsylvania MLSCN Grant: X01-MH076406-01 Target Factor XI (FXI) circulates as a complex with high molecular weight kininogen (HK) in the plasma at a concentration of 5 ug/ml (equivalent to 31.3 nM, dimeric concentration) as a homodimeric glycosylated blood plasma zymogen of approximately 160 kDa, containing monomeric subunits of 80 kDa each (1). Throm... aid798.table aid798.tbin
799 138738 Southern Research Molecular Libraries Screening Center (SRMLSC) Southern Research Institute (Birmingham, Alabama) NIH Molecular Libraries Screening Centers Network (MLSCN) Submitted by Dr. Gary A. Piazza (Southern Research Institute) in collaboration with Sharon Terry (Genetic Alliance) Proposal number 1X01-MH077620-01 Pseudoxanthoma elasticum (PXE) is a rare genetic disorder, which involves damage to connective tissues that result in multiple manifestations including skin abnormalities, blindne... aid799.table aid799.tbin
800 217502 Molecular Library Screening Center Network (MLSCN) Penn Center for Molecular Discovery (PCMD) Assay Provider: Scott L. Diamond, University of Pennsylvania MLSCN Grant: X01-MH076406-01 Target Factor XII (FXII) is a 80 kDa zymogen found at a concentration of 0.375 uM in plasma, and upon activation by kallikrein at R353, a disulfide-linked two chain molecule called factor XIIa alpha (FXIIa) is generated. FXIIa is also capable of autoactivation by binding to negatively charged surfaces (1). K... aid800.table aid800.tbin
801 26 NIH Molecular Libraries Screening Centers Network [MLSCN] Emory Chemical Biology Discovery Center in MLSCN Assay provider: Dr. Eric Sandberg, ZYGOGEN, LLC MLSCN Grant: 1 X01 MH077629-01 Assay Overview: Pathological angiogenesis contributes to over 70 diseases, including cancer, age-related macular degeneration and rheumatoid arthritis. Current in vitro models employed in screening compounds for effects on angiogenesis lack the biological complexity of in vivo systems. Zygogen, LLC developed a r... aid801.table aid801.tbin
802 94508 Cytokines such as TNF alpha and IL-1B activate a pro-inflammatory response in endothelial cells by nuclear translocation of the NFkB transcription factor (1). This response induces the transcription of pro-inflammatory genes including, at late times, the cell adhesion molecule, VCAM-1 (Vascular Cell Adhesion Molecule - 1) (2). The resulting cell surface expression of VCAM-1 upon stimulation with cytokines in primary human umbilical vein cells (HUVEC cells) provides a read-out to study the effec... aid802.table aid802.tbin
803 140109 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Affiliation: The Scripps Florida Research Institute, TSRI Assay Provider: Steven Brown, TSRI Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 1 R21 NS057101-01 PI: Steven Brown External Assay ID: GALR2_AG_BLA_1536_%ACT Name: Primary cell-based high-throughput screening assay to identify agonists of Galanin Receptor 2 (GALR2) Description: Galanin, a 29 amino acid neuropeptide... aid803.table aid803.tbin
804 138758 Southern Research Molecular Libraries Screening Center (SRMLSC) Southern Research Institute (Birmingham, Alabama) NIH Molecular Libraries Screening Centers Network (MLSCN) Assay Provider: Dr. David S. Goldfarb, University of Rochester Award: R03 MH076395-01 There is now solid evidence for the existence of conserved pathways that regulate cell aging and senescence. These pathways may have evolved to allow eukaryotic cells and animals to remain reproductively viable for long periods during unfavor... aid804.table aid804.tbin
805 15 Principal Investigator: Bruce S. Edwards, Ph.D (BEdwards@salud.unm.edu) Grant: NIH 1R03MH076381-01 Screening Center: New Mexico Molecular Libraries Screening Center Background/Significance Formyl peptide receptors. The G-protein coupled formylpeptide receptor(FPR) was one of the originating members of the chemoattractant receptor superfamily (1, 2). N-formylated peptides such as fMLF are high affinity FPR ligands that trigger a variety of biologic activities in myeloid cells, including chemoki... aid805.table aid805.tbin
806 305 Southern Research Molecular Libraries Screening Center (SRMLSC) Southern Research Institute (Birmingham, Alabama) NIH Molecular Libraries Screening Centers Network (MLSCN) Assay Provider: Dr. William E. Severson, Southern Research Institute Award: R03 MH081270-01 Currently, there is no commercially available vaccine to protect humans against the highly pathogenic avian influenza H5N1 virus that is spreading across Asia, Europe, and Africa. Since humans have no immunity against any H5 viruses, th... aid806.table aid806.tbin
807 9532 Southern Research Molecular Libraries Screening Center (SRMLSC) Southern Research Institute (Birmingham, Alabama) NIH Molecular Libraries Screening Centers Network (MLSCN) Submitted by Dr. Gary A. Piazza (Southern Research Institute) in collaboration with Sharon Terry (Genetic Alliance) Proposal number 1X01-MH077620-01 Pseudoxanthoma elasticum (PXE) is a rare genetic disorder, which involves damage to connective tissues that result in multiple manifestations including skin abnormalities, blindne... aid807.table aid807.tbin
808 92911 Cytokines such as TNF alpha and IL-1B activate a pro-inflammatory response in endothelial cells by nuclear translocation of the NFkB transcription factor (1). This response induces the transcription of pro-inflammatory genes including, at late times, the cell adhesion molecule, VCAM-1 (Vascular Cell Adhesion Molecule - 1) (2). The resulting cell surface expression of VCAM-1 upon stimulation with cytokines in primary human umbilical vein cells (HUVEC cells) provides a read-out to study the effec... aid808.table aid808.tbin
809 499 Southern Research Molecular Libraries Screening Center (SRMLSC) Southern Research Institute (Birmingham, Alabama) NIH Molecular Libraries Screening Centers Network (MLSCN) Assay Provider: Dr. David S. Goldfarb, University of Rochester Award: R03 MH076395-01 There is now solid evidence for the existence of conserved pathways that regulate cell aging and senescence. These pathways may have evolved to allow eukaryotic cells and animals to remain reproductively viable for long periods during unfavor... aid809.table aid809.tbin
810 210 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute, TSRI Assay Provider: Scripps Florida Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: Not Applicable PI: Peter Hodder External Assay ID: FAK_INH_TRFRET_1536_IC50 Name: Dose-response biochemical assay for inhibitors of Focal Adhesion Kinase (FAK) Description: The focal adhesion kinase (FAK) is a tyrosine kinase involved i... aid810.table aid810.tbin
811 15680 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute, TSRI Assay Provider: The Scripps Research Institute Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 1R03MH081265-01 PI: PI Peter Tobias External Assay ID: TLR4-MYD88_Antagonist BLA Enzyme Complementation Name: Primary Cell Based High Throughput Screening Assay for Inhibitors of TLR4-MyD88 binding Description In atheroscleros... aid811.table aid811.tbin
812 499 Southern Research Molecular Libraries Screening Center (SRMLSC) Southern Research Institute (Birmingham, Alabama) NIH Molecular Libraries Screening Centers Network (MLSCN) Assay Provider: Dr. David S. Goldfarb, University of Rochester Award: R03 MH076395-01 There is now solid evidence for the existence of conserved pathways that regulate cell aging and senescence. These pathways may have evolved to allow eukaryotic cells and animals to remain reproductively viable for long periods during unfavor... aid812.table aid812.tbin
813 64394 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Proposal Number: 1R03 MH082385-01 Alkaline phosphatases (EC 3.1.3.1) (APs) catalyze the hydrolysis of phosphomonoesters, releasing phosphate and alcohol. APs are dimeric enzymes found in the most organisms. In human, four isozymes of APs have been identified. Three isozymes ... aid813.table aid813.tbin
814 24 Data Source: SRMLSC (Her2) Southern Research Molecular Libraries Screening Center (SRMLSC) Southern Research Institute (Birmingham, Alabama) NIH Molecular Libraries Screening Centers Network (MLSCN) Assay Provider: Dr. Gabriela Chiosis, Memorial Sloan-Kettering Cancer Center Award: 1R03MH076408-012 Her2 (ErbB2) protein is over-expressed in breast and other solid tumors and is often mutated in patients with progressive disease. Her2 is a member of a family of four transmembrane tyrosine kinase r... aid814.table aid814.tbin
815 34 Emory Chemistry-Biology Discovery Center Assay Overview: Grant number: none Paramyxoviruses comprise several major human pathogens. Although a live-attenuated vaccine protects against measles virus (MV), a member of the paramyxovirus family, the virus remains a principal cause of worldwide mortality and accounts for approximately 21 million cases and 300,000 to 400,000 deaths annually. The development of novel antivirals that allow improved case management of severe measles and silence viral out... aid815.table aid815.tbin
816 499 Southern Research Molecular Libraries Screening Center (SRMLSC) Southern Research Institute (Birmingham, Alabama) NIH Molecular Libraries Screening Centers Network (MLSCN) Assay Provider: Dr. David S. Goldfarb, University of Rochester Award: R03 MH076395-01 There is now solid evidence for the existence of conserved pathways that regulate cell aging and senescence. These pathways may have evolved to allow eukaryotic cells and animals to remain reproductively viable for long periods during unfavor... aid816.table aid816.tbin
817 138758 NIH Molecular Libraries Screening Centers Network [MLSCN] Emory Chemical Biology Discovery Center in MLSCN Assay provider: Dr. Paul De Figueiredo, Texas A&M University System MLSCN Grant: 1 X01 MH079865-01 Assay Overview: Shwachman-Diamond Syndrome (SDS) is an autosomal recessive genetic syndrome that results in hematopoietic defects as well as impaired pancreatic function and skeletal development (1, 2). Presently, therapeutic options are limited to supportive care or bone marrow transplant, ... aid817.table aid817.tbin
818 138758 Southern Research Molecular Libraries Screening Center (SRMLSC) Southern Research Institute (Birmingham, Alabama) NIH Molecular Libraries Screening Centers Network (MLSCN) Submitted by Dr. Todd Waldman (Lombardi Cancer Center, Georgetown University School of Medicine) Award: R03-NS053741 Numerous genes have been identified which participate, either through activation (oncogenes) or inactivation (tumor suppressors), in the multifactorial process of carcinogenesis (Vogelstein B and Kinzler KW, 200... aid818.table aid818.tbin
819 94241 Inflammatory disease requires that endothelial cells detect and amplify a pro-inflammatory signal. This results in the adherence, activation, and ultimately transmigration of lymphocytes at the site of damage (Pober, 2002). This assay detects an early event in this process, the nuclear translocation of the transcription factor, NFkappaB (Senftleben, et al., 2002). Chronic inflammatory disease is believed to pose a tremendous medical burden in the developed world, not only in terms of patient suf... aid819.table aid819.tbin
820 75 Screening Center: Penn Center for Molecular Discovery Center Affiliation: University of Pennsylvania Network: Molecular Library Screening Center Network (MLSCN) Assay Provider: Scott Diamond, University of Pennsylvania Grant number: MH076406-01 Human liver cathepsin B (EC 3.4.22.1) is a lysosomal cysteine protease. There has been a recent resurgence of interest in cathepsin B due to research showing that proteolysis by this enzyme is required for the entry and replication of the Ebola and SARS v... aid820.table aid820.tbin
821 697 Southern Research Molecular Libraries Screening Center (SRMLSC) Southern Research Institute (Birmingham, Alabama) NIH Molecular Libraries Screening Centers Network (MLSCN) Assay Provider: Dr. Zhican Qu, Southern Research Institute Award: X01MH079851-01 Angiogenesis is the process of new blood vessel formation, which is believed to be involved in many human diseases including cancer, diabetic retinopathy, and rheumatoid arthritis (Carmeliet, 2005). Endothelial cell proliferation is known to occur... aid821.table aid821.tbin
822 697 Southern Research Molecular Libraries Screening Center (SRMLSC) Southern Research Institute (Birmingham, Alabama) NIH Molecular Libraries Screening Centers Network (MLSCN) Assay Provider: Dr. Zhican Qu, Southern Research Institute Award: X01MH079851-01 Angiogenesis is a process of new blood vessel formation. Endothelial cell proliferation is an essential step during the angiogenesis process and is involved in many human diseases including cancer, diabetic retinopathy, and rheumatoid arthritis (C... aid822.table aid822.tbin
823 9532 Southern Research Molecular Libraries Screening Center (SRMLSC) Southern Research Institute (Birmingham, Alabama) NIH Molecular Libraries Screening Centers Network (MLSCN) Submitted by Dr. Todd Waldman (Lombardi Cancer Center, Georgetown University School of Medicine) Award: R03-NS053741 Numerous genes have been identified which participate, either through activation (oncogenes) or inactivation (tumor suppressors), in the multifactorial process of carcinogenesis (Vogelstein B and Kinzler KW, 200... aid823.table aid823.tbin
824 9532 Southern Research Molecular Libraries Screening Center (SRMLSC) Southern Research Institute (Birmingham, Alabama) NIH Molecular Libraries Screening Centers Network (MLSCN) Submitted by Dr. Todd Waldman (Lombardi Cancer Center, Georgetown University School of Medicine) Award: 1R03NS050857-01 Numerous genes have been identified which participate, either through activation (oncogenes) or inactivation (tumor suppressors), in the multifactorial process of carcinogenesis (Vogelstein B and Kinzler KW, ... aid824.table aid824.tbin
825 102 Screening Center: Penn Center for Molecular Discovery Center Affiliation: University of Pennsylvania Network: Molecular Library Screening Center Network (MLSCN) Assay Provider: Scott Diamond, University of Pennsylvania Grant number: MH076406-01 Human liver cathepsin L (EC 3.4.22.15) is a lysosomal cysteine protease. Recent interest in cathepsin L has been generated by research showing that proteolysis by this enzyme is required for the entry and replication of the SARS and Ebola viruses in human... aid825.table aid825.tbin
826 105 Screening Center: Penn Center for Molecular Discovery Center Affiliation: University of Pennsylvania Network: Molecular Library Screening Center Network (MLSCN) Assay Provider: Arkady Mustaev, Public Health Research Institute, Newark, NJ Grant number: MH076325-01 DNA-directed RNA polymerase (EC 2.7.7.6) is responsible for bacterial RNA synthesis and as such is essential for bacterial gene expression. Owing to its central role in DNA transcription, the enzyme RNA polymerase is the target of vario... aid826.table aid826.tbin
827 138438 Southern Research Molecular Libraries Screening Center (SRMLSC) Southern Research Institute (Birmingham, Alabama) NIH Molecular Libraries Screening Centers Network (MLSCN) Submitted by Dr. Todd Waldman (Lombardi Cancer Center, Georgetown University School of Medicine) Award: 1R03NS050857-01 Numerous genes have been identified which participate, either through activation (oncogenes) or inactivation (tumor suppressors), in the multifactorial process of carcinogenesis (Vogelstein B and Kinzler KW, ... aid827.table aid827.tbin
828 140109 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Affiliation: The Scripps Florida Research Institute, TSRI Assay Provider: Steven Brown, TSRI Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 1 R21 NS057101-01 PI: Steven Brown External Assay ID: GALR2_ANT_BLA_1536_%INH Name: Primary cell-based high-throughput screening assay to identify antagonists of Galanin Receptor 2 (GALR2) Description: Galanin, a 29 amino acid neurope... aid828.table aid828.tbin
829 21 Molecular Library Screening Center Network (MLSCN) Penn Center for Molecular Discovery (PCMD) Assay Provider: Scott L. Diamond, University of Pennsylvania MLSCN Grant: X01-MH076406-01 The classical pathway mediates specific antibody responses. The classical pathway is initiated by the binding of antibodies to cell surface antigens. Subsequent binding of the antibody to complement C1q subunits of C1 result in catalytically active C1s subunits. The two activated C1s subunits are then able to cata... aid829.table aid829.tbin
830 56 Screening Center: Penn Center for Molecular Discovery Center Affiliation: University of Pennsylvania Network: Molecular Library Screening Center Network (MLSCN) Assay Provider: Scott Diamond, University of Pennsylvania Grant number: MH076406-01 One of our goals at the Penn Center for Molecular Discovery (PCMD) is to develop capabilities for screening multiple members of target classes, for example cysteine and serine proteases. Many HTS labs focus effort on one target of interest within a class ... aid830.table aid830.tbin
831 129 Screening Center: Penn Center for Molecular Discovery Center Affiliation: University of Pennsylvania Network: Molecular Library Screening Center Network (MLSCN) Assay Provider: Scott Diamond, University of Pennsylvania Grant number: MH076406-01 Human cathepsin S (EC 3.4.22.27) is a lysosomal cysteine protease that is expressed in antigen-presenting cells, especially dendritic cells, B-cells and macrophages. Cathepsin S plays a key role in the processing of antigenic peptides for presentation by ... aid831.table aid831.tbin
832 93 Screening Center: Penn Center for Molecular Discovery Center Affiliation: University of Pennsylvania Network: Molecular Library Screening Center Network (MLSCN) Assay Provider: Scott Diamond, University of Pennsylvania Grant number: MH076406-01 Cathepsin G (EC 3.4.21.20) is a chymotrypsin-like serine protease that is secreted from neutrophils. Disregulated cathepsin G activity is implicated in the progression of various chronic inflammatory diseases such as asthma and chronic pulmonary obstructi... aid832.table aid832.tbin
833 16000 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute, TSRI Assay Provider: Steven Brown, TSRI Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 1R21NS057101-01 PI: Steven Brown External Assay ID: GALR2_Agonist_BLAReporter_MaybridgeHitFinder_PrimaryScreen Name: Primary Cell Based High Throughput Screening Assay for Agonists of GALR2 Description: Galanin is a 29 amino acid neuro... aid833.table aid833.tbin
834 84889 Molecular Library Screening Center Network (MLSCN) Penn Center for Molecular Discovery (PCMD) Assay Provider: Kim Lewis, Northeastern University, Boston, MA MLSCN Grant: X01 MH080686-01 This screen is for compounds that are potentiators of the antifungal drug clotrimazole that are active against multidrug tolerant persister cells of Candida albicans biofilms. Biofilms are notoriously resistant to antimicrobial therapy, but the mechanism of resistance remains largely unknown. The recently charact... aid834.table aid834.tbin
835 128 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute, TSRI Assay Provider: Peter Kuhn, TSRI Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 1 X01 MH079857-01 Grant Proposal PI: Peter Kuhn External Assay ID: EphB4TNYLRAW_INH_FP_1536_IC50 Name: Dose-response biochemical assay for antagonists of the interaction between the Eph receptor B4 (EphB4) and its ligand ephrin-B2 via TNYL... aid835.table aid835.tbin
836 91307 Data Source: Columbia University Molecular Screening Center Source (MLSCN Center Name): Columbia University Molecular Screening Center Center Affiliation: Columbia University Molecular Screening Center Assay Provider: Thomas Mayer, Columbia University Molecular Screening Center Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 1R03 MH076343-01 During inflammation, cytokine activation of the NFkB signaling pathway results in, among others, VCAM-1 (Vascular Cell ... aid836.table aid836.tbin
837 171 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute, TSRI Assay Provider: James R. Williamson, TSRI Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 1 X01 MH078935-01 Grant Proposal PI: James R. Williamson External Assay ID: HIVREVRRE_INH_FRET_1536_3X%INH Name: Confirmation biochemical assay for inhibitors of the HIV Rev-RRE RNA interaction (disruption of protein-RNA interact... aid837.table aid837.tbin
838 50 Southern Research Molecular Libraries Screening Center (SRMLSC) Southern Research Institute (Birmingham, Alabama) NIH Molecular Libraries Screening Centers Network (MLSCN) Award: 1R03MH076412-01 Multi-drug resistant Mycobacterium tuberculosis is becoming an increased health problem, especially in immunocompromised individuals with HIV. This form of TB is more difficult to treat and as a result has a higher mortality rate. Because of this, the discovery of drugs targeting novel pathways such as t... aid838.table aid838.tbin
839 24 Southern Research Molecular Libraries Screening Center (SRMLSC) Southern Research Institute (Birmingham, Alabama) NIH Molecular Libraries Screening Centers Network (MLSCN) Assay Provider: Dr. Edina Harsay, University of Kansas Award: X01-MH077628-01 Chemical Genetic Screens to Identify Modulators of Post-Golgi Transport The intracellular transport and targeted delivery of cell surface and secreted proteins is a fundamental process in all eukaryotic cells. The regulation of membrane transport pa... aid839.table aid839.tbin
840 271 We analyzed the RNAi induced by a large subset of our siRNA library. The targets of this library include genes with a broad range of known and putative cancer-related functions including classical oncogenes and tumor suppressors, established and putative anti-cancer targets (primary and metastatic) and proteins associated with anti-cancer chemotherapeutic responses. Overall, we characterized the target specific effects of 258 synthetic siRNAs on mRNA levels corresponding to 129 human genes. To co... aid840.table aid840.tbin
841 137798 Emory Chemistry-Biology Discovery Center Assay Overview: Grant number: none Paramyxoviruses comprise several major human pathogens. Although a live-attenuated vaccine protects against measles virus (MV), a member of the paramyxovirus family, the virus remains a principal cause of worldwide mortality and accounts for approximately 21 million cases and 300,000 to 400,000 deaths annually. The development of novel antivirals that allow improved case management of severe measles and silence viral out... aid841.table aid841.tbin
842 28 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute, TSRI Assay Provider: James R. Williamson, TSRI Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 1 X01 MH078935-01 Grant Proposal PI: James R. Williamson External Assay ID: HIVREVRRE_INH_FRET_1536_ IC50 Name: Dose response biochemical assay to identify inhibitors of the HIV Rev - RRE RNA interaction (disruption of protein-RNA... aid842.table aid842.tbin
843 63 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute (TSRI) Assay Provider: Hugh Rosen, TSRI Grant Proposal Number: 1 R03 MH076533-01 Grant Proposal PI: Germana Sanna External Assay ID: Counterscreen for S1P2 Agonists: Dose Response High Throughput Cell-Based Screen to Identify Activators of CRE-BLA Name: CRE_AG_BLA_384_EC50_%ACT Description: Sphingosine 1-phosphate (S1P) influences heart rate [1,2], corona... aid843.table aid843.tbin
844 803 Southern Research Molecular Libraries Screening Center (SRMLSC) Southern Research Institute (Birmingham, Alabama) NIH Molecular Libraries Screening Centers Network (MLSCN) Award 1X01-MH077620-01: Submitted by Dr. Gary A. Piazza (Southern Research Institute) in collaboration with Sharon Terry (Genetic Alliance) Pseudoxanthoma elasticum (PXE) is a rare genetic disorder (Bergen, 2007), which involves damage to connective tissues that result in multiple manifestations including skin abnormalities, b... aid844.table aid844.tbin
845 94241 Data Source: Columbia University Molecular Screening Center Source (MLSCN Center Name): Columbia University Molecular Screening Center Center Affiliation: Columbia University Molecular Screening Center Assay Provider: Thomas Mayer, Columbia University Molecular Screening Center Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 1R03MH076344-01 Inflammatory disease requires that endothelial cells detect and amplify a pro-inflammatory signal. This results in the adh... aid845.table aid845.tbin
846 302 Molecular Library Screening Center Network (MLSCN) Penn Center for Molecular Discovery (PCMD) Assay Provider: Scott L. Diamond, University of Pennsylvania MLSCN Grant: X01-MH076406-01 Target Factor XI (FXI) circulates as a complex with high molecular weight kininogen (HK) in the plasma at a concentration of 5 ug/ml (equivalent to 31.3 nM, dimeric concentration) as a homodimeric glycosylated blood plasma zymogen of approximately 160 kDa, containing monomeric subunits of 80 kDa each (1). Throm... aid846.table aid846.tbin
847 41218 Southern Research Molecular Libraries Screening Center (SRMLSC) Southern Research Institute (Birmingham, Alabama) NIH Molecular Libraries Screening Centers Network (MLSCN) (Proposal number 1R03MH076408-012) Submitted by Gabriela Chiosis of the Memorial Sloan-Kettering Institute for Cancer Research Compounds that inhibit tumor cell growth may have cytotoxic or cytostatic effects and vary widely in structure and mechanism of action. Cancer chemotherapeutic drugs inhibit tumor cell growth by disru... aid847.table aid847.tbin
848 92911 Data Source: Columbia University Molecular Screening Center Source (MLSCN Center Name): Columbia University Molecular Screening Center Center Affiliation: Columbia University Molecular Screening Center Assay Provider: Thomas Mayer, Columbia University Molecular Screening Center Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 1R03 MH076343-01 During inflammation, cytokine activation of the NFkB signaling pathway results in, among others, VCAM-1 (Vascular Cell A... aid848.table aid848.tbin
849 759 Southern Research Molecular Libraries Screening Center (SRMLSC) Southern Research Institute (Birmingham, Alabama) NIH Molecular Libraries Screening Centers Network (MLSCN) Assay Provider: Dr. David S. Goldfarb, University of Rochester Award: R03 MH076395-01 There is now solid evidence for the existence of conserved pathways that regulate cell aging and senescence. These pathways may have evolved to allow eukaryotic cells and animals to remain reproductively viable for long periods during unfavor... aid849.table aid849.tbin
850 759 Southern Research Molecular Libraries Screening Center (SRMLSC) Southern Research Institute (Birmingham, Alabama) NIH Molecular Libraries Screening Centers Network (MLSCN) Assay Provider: Dr. David S. Goldfarb, University of Rochester Award: R03 MH076395-01 There is now solid evidence for the existence of conserved pathways that regulate cell aging and senescence. These pathways may have evolved to allow eukaryotic cells and animals to remain reproductively viable for long periods during unfavor... aid850.table aid850.tbin
851 82 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute (TSRI) Assay Provider: Hugh Rosen, TSRI Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 1 R03 MH076533-01 Grant Proposal PI: Germana Sanna External Assay ID: S1P2_ANT_BLA_384_IC50 Name: Dose Response Cell Based Assay for Antagonists of the S1P2 Receptor Description: Sphingosine 1-phosphate (S1P) influences heart rate [1,2... aid851.table aid851.tbin
852 649 Molecular Library Screening Center Network (MLSCN) Penn Center for Molecular Discovery (PCMD) Assay Provider: Scott L. Diamond, University of Pennsylvania MLSCN Grant: X01-MH076406-01 Target Factor XII (FXII) is a 80 kDa zymogen found at a concentration of 0.375 uM in plasma, and upon activation by kallikrein at R353, a disulfide-linked two chain molecule called factor XIIa alpha (FXIIa) is generated. FXIIa is also capable of autoactivation by binding to negatively charged surfaces (1). K... aid852.table aid852.tbin
853 46736 Data Source: Columbia University Molecular Screening Center Source (MLSCN Center Name): Columbia University Molecular Screening Center Center Affiliation: Columbia University Molecular Screening Center Assay Provider: Drs. A Yamamoto and JE Rothman, Department of Physiology and Cellular Biophysics at Columbia University. Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 1R03MH076348-01 The neurodegenerative disorder Huntington's Disease is caused by a polyglutami... aid853.table aid853.tbin
854 63 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute (TSRI) Assay Provider: Hugh Rosen, TSRI Grant Proposal Number: 1 R03 MH076533-01 Grant Proposal PI: Germana Sanna External Assay ID: S1P2_AG_BLA_384_EC50 Name: Dose Response Cell Based Assay for Agonists of the S1P2 Receptor Description: Sphingosine 1-phosphate (S1P) influences heart rate [1, 2], coronary artery caliber, endothelial integrity, lung epithe... aid854.table aid854.tbin
855 564 Dose Response Confirmation for Small Molecule Inhibitors of Eukaryotic Translation Initiation NIH Molecular Libraries Screening Centers Network [MLSCN] Emory Chemical Biology Discovery Center in MLSCN Assay provider: Dr. Jerry Pelletier, McGill UNIVERSITY MLSCN Grant: 1 R03 MH081216-01 Title: Dose Response Confirmation for Small Molecule Inhibitors of Eukaryotic Translation Initiation Assay Overview The recruitment of the 40S ribosomal subunit and associated factors (43S pre-initiation complex... aid855.table aid855.tbin
856 82 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute (TSRI) Assay Provider: Hugh Rosen, TSRI Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 1 R03 MH076533-01 Grant Proposal PI: Germana Sanna External Assay ID: CRE _ANT_BLA_384_IC50_Counterscreen_ S1P2_Hits Name: Counterscreen for S1P2 Antagonists: Dose Response Cell-Based Screen to Identify Antagonists of CRE-BLA Descript... aid856.table aid856.tbin
857 457 Assay Provider: Val Watts Assay Provider Affiliation: Purdue University Grant Title: Allosteric Modulators of D1 Receptors Grant Number: 1 X01 MH077619-01 Dopamine receptors have been classified into two large families, the D1-like and the D2-like (Neve et al., 2004). Members of the D1-like receptor family include D1 and D5 dopamine receptors. Activation of D1-like receptors stimulate Gs which in turn activates adenylate cyclase resulting in enhanced cyclic AMP accumulation (Neve et al., 2004). ... aid857.table aid857.tbin
858 517 Assay Provider: Val Watts Assay Provider Affiliation: Purdue University Grant Title: Allosteric Modulators of D1 Receptors Grant Number: 1 X01 MH077619-01 Dopamine receptors have been classified into two large families, the D1-like and the D2-like (Neve et al., 2004). Members of the D1-like receptor family include D1 and D5 dopamine receptors. Activation of D1-like receptors stimulate Gs which in turn activates adenylate cyclase resulting in enhanced cyclic AMP accumulation (Neve et al., 2004). ... aid858.table aid858.tbin
859 591 Assay Provider: P. Jeffrey Conn Assay Provider Affiliation: Vanderbilt University Grant Title: Discovery of novel allosteric modulators of the M1 muscarinic receptor Grant Number: 1 R03 MH077606-01 The M1 muscarinic receptor is thought to be an important therapeutic target in schizophrenia. A cell-based fluorometric calcium assay was developed for high throughput screening. This assay was used to identify compounds with high selectivity for the M1 receptor subtype that act at an allosteric site ... aid859.table aid859.tbin
860 719 Assay Provider: P. Jeffrey Conn Assay Provider Affiliation: Vanderbilt University Grant Title: Discovery of novel allosteric modulators of the M1 muscarinic receptor Grant Number: 1 R03 MH077606-01 The M1 muscarinic receptor is thought to be an important therapeutic target in schizophrenia. A cell-based fluorometric calcium assay was developed for high throughput screening. This assay was used to identify compounds with high selectivity for the M1 receptor subtype that act at an allosteric site ... aid860.table aid860.tbin
861 196012 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute, TSRI Assay Provider: Peter Tobias, TSRI Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 1 X01 MH079857-01 Grant Proposal PI: Peter Tobias External Assay ID: TLR4-MyD88 _INH_ BLA_1536_%INH Name: Primary cell-based high-throughput screening assay for inhibitors of TLR4-MyD88 binding Description: In atherosclerosis, kidney t... aid861.table aid861.tbin
862 194698 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute (TSRI) Assay Provider: David Frank, Dana-Farber Cancer Institute Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 1 X01 MH079826-01 Grant Proposal PI: David Frank External Assay ID: STAT3_INH_LUMI_1536_%INH Name: Primary cell-based high throughput screening assay to identify inhibitors of STAT3 Description: The signal... aid862.table aid862.tbin
863 15 Principal Investigator: Bruce S. Edwards, Ph.D Grant: NIH 1R03MH076381-01 Screening Center: New Mexico Molecular Libraries Screening Center Assay Background and Significance Formyl peptide receptors. The G-protein coupled formylpeptide receptor (FPR) was one of the originating members of the chemoattractant receptor superfamily (Le et al., 2002a; Oppenheim et al., 1991). N-formylated peptides such as fMLF are high affinity FPR ligands that trigger a variety of biologic activities in myeloid ce... aid863.table aid863.tbin
864 455 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Affiliation: The Scripps Florida Research Institute, TSRI Assay Provider: Steven Brown, TSRI Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 1 R21 NS057101-01 Grant Proposal PI: Steven Brown External Assay ID: GALR2_AG_BLA_1536_3X%ACT Name: Confirmatory cell-based high-throughput screening assay to identify agonists of galanin receptor 2 (GALR2) Description: Galanin, a 29 a... aid864.table aid864.tbin
865 455 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Affiliation: The Scripps Florida Research Institute (TSRI) Assay Provider: Steven Brown, TSRI Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 1 R21 NS057101-01 Grant Proposal PI: Steven Brown External Assay ID: NFAT_ACT_BLA_1536_3X%ACT Name: Counterscreen for agonists of galanin receptor 2 (GalR2): a cell-based high-throughput screening assay for activators of beta-lactamase ... aid865.table aid865.tbin
866 478 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Affiliation: The Scripps Research Institute (TSRI) Assay Provider: Steven Brown, TSRI Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 1 R21 NS057 101-01 Grant Proposal PI: Steven Brown External Assay ID: GALR2_ANT_BLA_1536_3X%INH Name: Confirmatory cell-based high-throughput screening assay to identify antagonists of galanin receptor 2 (GALR2) Description: Galanin, a 29 ami... aid866.table aid866.tbin
867 478 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Affiliation: The Scripps Research Institute (TSRI) Assay Provider: Steven Brown, TSRI Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 1 R21 NS057101-01 Grant Proposal PI: Steven Brown External Assay ID: NFAT_INH_BLA_1536_3X%INH Name: Counterscreen for antagonists of galanin receptor 2 (GalR2): a cell-based high-throughput screening assay for inhibitors of beta-lactamase activ... aid867.table aid867.tbin
868 194582 Southern Research Molecular Libraries Screening Center (SRMLSC) Southern Research Institute (Birmingham, Alabama) NIH Molecular Libraries Screening Centers Network (MLSCN) Assay Provider: Dr. Eric Weiss, Northwestern University Award: 1R21NS056968-01 A screen for inhibitors of the budding yeast RAM network The RAM network is a novel and highly conserved signaling pathway involved in maintenance of polarized growth, cell proliferation, and control of gene expression. The pathway is well conserve... aid868.table aid868.tbin
869 9532 Southern Research Molecular Libraries Screening Center (SRMLSC) Southern Research Institute (Birmingham, Alabama) NIH Molecular Libraries Screening Centers Network (MLSCN) Assay Provider: Dr. Eric Weiss, Northwestern University Award: 1R21NS056968-01 A screen for inhibitors of the budding yeast RAM network The RAM network is a novel and highly conserved signaling pathway involved in maintenance of polarized growth, cell proliferation, and control of gene expression. The pathway is well conserve... aid869.table aid869.tbin
871 194698 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute (TSRI) Assay Provider: David Frank, Dana Farber Cancer Institute Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 1 X01 MH079826-01 Grant Proposal PI: David Frank External Assay ID: STAT3_POT_LUC_1536_%ACT Name: Primary cell-based high throughput screening assay to measure STAT3 activation. Description: The signal transdu... aid871.table aid871.tbin
872 59 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute (TSRI) Assay Provider: Hugh Rosen, TSRI Grant Proposal Number: 1 R03 MH076533-01 Grant Proposal PI: Germana Sanna External Assay ID: S1P2_AG_BLA_384_EC50_Purchased_Analogues Name: Dose Response Cell Based Assay for Agonists of the S1P2 Receptor of Purchased Analogues Description: Sphingosine 1-phosphate (S1P) influences heart rate [1, 2], coronary artery ca... aid872.table aid872.tbin
873 214261 Molecular Library Screening Center Network (MLSCN) Penn Center for Molecular Discovery (PCMD) Assay Provider: Scott L. Diamond, University of Pennsylvania MLSCN Grant: X01-MH076406-01 Target Human kallikrein 5 (hK5) is a member of the human tissue kallikrein family, which contains 15 kallikrein-like serine proteases (1). It is synthesized as a 293 amino acid zymogen, and loses a 29 amino acid signal peptide upon secretion, followed by cleavage at the Arg66-Ile67 bond, which releases a 37 ami... aid873.table aid873.tbin
874 59 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute (TSRI) Assay Provider: Hugh Rosen, TSRI Grant Proposal Number: 1 R03 MH076533-01 Grant Proposal PI: Germana Sanna External Assay ID: CRE_AG_BLA_384_EC50_S1P2_Purchased_Analogues Name: Counterscreen for S1P2 Agonists: Dose Response High Throughput Cell-Based Screen to Identify Activators of CRE-BLA: S1P2 Purchased Analogues Description: Sphingosine 1-phosphat... aid874.table aid874.tbin
875 75028 NIH Molecular Libraries Screening Centers Network [MLSCN] NIH Chemical Genomics Center [NCGC] MLSCN Grant: MH081227-01 PI Name: Natarajan, Amarnath; University of Texas Inhibitors of the BRCT:pBACH1 interaction should prove useful in studies of BRCA1's tumor suppression role and to potentially sensitive tumors to chemotherapeutic agents. A complex of BRCT (C-terminal portion of BRCA1, MW ~35 kDa, His-tagged) and fluorescently labeled pBACH1 phosphorylated 10 aa peptide fragment of BACH1, a helic... aid875.table aid875.tbin
876 119 List of compounds to be tested: Compounds that met the active criterion of Z-score is </= -3 in the in vitro PLK1-PBD binding primary screen AID 693, will be tested in 10-point concentration response assays in the presence of 0.5 mM DTT. The PLK-1-PBD hit characterization compound concentration dependent redox cycling H2O2 generation assay in the presence of 0.5 mM DTT, has been developed to evaluate actives that were identified in the in vitro PLK1-PBD binding primary screen AID 693, conducte... aid876.table aid876.tbin
877 117 List of compounds to be tested: Compounds that met the active criterion of Z-score is </= -3 in the in vitro PLK1-PBD binding primary screen AID 693, will be confirmed in 10-point concentration response assays. Extracted from the MH078944 proposal submitted by Dr. Michael Yaffe of MIT. The Polo-like kinases (Plks) play important roles in many cell cycle-related events including the initiation of mitosis, chromosome segregation, centrosome maturation, bipolar spindle formation, regulation of an... aid877.table aid877.tbin
878 195853 Hydrogen peroxide (H2O2) can modulate (activate or inhibit) the activity of a variety of proteins including protein kinases, protein phosphatases, transcription factors, phospholipases, ion channels and G proteins. H2O2 is capable of oxidizing the cysteine residues of proteins that may be crucial for their catalytic and/or structural function. Many proteins, including a significant number of the targets screened by the MLSCN, contain an active site cysteine that is required for biological activi... aid878.table aid878.tbin
879 1279 Assay Overview: NIH Molecular Libraries Screening Centers Network [MLSCN] NIH Chemical Genomics Center [NCGC] MLSCN Grant: NS053754-01 Assay Provider: Siderovski, David P, University of North Carolina at Chapel Hill G-protein-coupled receptors (GPCRs) are major targets for drug discovery. The regulator of G-protein signaling (RGS)-protein family has important roles in GPCR signal transduction. RGS proteins contain a conserved RGS-box, which is often accompanied by other signaling regulatory ele... aid879.table aid879.tbin
880 234161 Assay Submitter: Siderovski, David P, University of North Carolina at Chapel Hill Screening Center PI: Austin, C.P. Screening Center: NIH Chemical Genomics Center [NCGC] NIH Grant: NS053754-01 G-protein-coupled receptors (GPCRs) are major targets for drug discovery. The regulator of G-protein signaling (RGS)-protein family has important roles in GPCR signal transduction. RGS proteins contain a conserved RGS-box, which is often accompanied by other signaling regulatory elements. RGS proteins acce... aid880.table aid880.tbin
881 108408 Assay Overview: NIH Molecular Libraries Screening Centers Network [MLSCN] NIH Chemical Genomics Center [NCGC] MLSCN Grant: MH081283-01A1 PI Name: Holman, T.R., University of California, Santa Cruz Human lipoxygenase 15hLO-2 is a member of the closely related lipoxygenase family of enzymes which catalyze the site-specific oxidation of arachidonic acid to various hormone precursor molecules and as such is a candidate for drug development in a variety of disease areas, such as cancer and inflammat... aid881.table aid881.tbin
883 10320 NIH Molecular Libraries Screening Centers Network [MLSCN] NIH Chemical Genomics Center [NCGC] MLSCN Grant: None NCGC Assay Overview: The P450 gene superfamily is involved in the metabolism and clearance of xenobiotics. This assay used human CYP2C9 to measure the hydroxylation of deoxyluciferin (Luciferin-H; P450 Glo-Buffer) to luciferin. The luciferin is then measured by luminescence after the addition of a luciferase detection reagent. Luciferin-H concentration in the assay was equal to its M... aid883.table aid883.tbin
884 14155 NIH Molecular Libraries Screening Centers Network [MLSCN] NIH Chemical Genomics Center [NCGC] MLSCN Grant: None NCGC Assay Overview: The P450 gene superfamily is involved in the metabolism and clearance of xenobiotics. This assay used human CYP3A4 to measure the dealkylation of luciferin-6' phenylpiperazinylyl (Luciferin-PPXE; luciferin detection buffer) to luciferin. The luciferin is then measured by luminescence after the addition of a luciferase detection reagent. Luciferin-PPXE concentrati... aid884.table aid884.tbin
885 14155 NIH Molecular Libraries Screening Centers Network [MLSCN] NIH Chemical Genomics Center [NCGC] MLSCN Grant: None NCGC Assay Overview: The P450 gene superfamily is involved in the metabolism and clearance of xenobiotics. This assay used human CYP3A4 to measure the dealkylation of luciferin-6' phenylpiperazinylyl (Luciferin-PPXE; luciferin detection buffer) to luciferin. The luciferin is then measured by luminescence after the addition of a luciferase detection reagent. Luciferin-PPXE concentrati... aid885.table aid885.tbin
886 73252 Assay Overview: NIH Molecular Libraries Screening Centers Network [MLSCN] NIH Chemical Genomics Center [NCGC] Structural Genomics Consortium [SGC] Grant Number: None HADH2: Hydroxyacyl-Coenzyme A dehydrogenase, type II, was supplied by researchers at the Structural Genomics Consortium, Oxford University lab (PI Udo Oppermann). The mitochondrial enzyme 17-hydroxysteroid dehydrogenase type 10 (HSD17-10) previously classified as type II hydroxyacyl-CoA dehydrogenase (HADH2) catalyzes the NAD+ depe... aid886.table aid886.tbin
887 74290 Assay Overview: NIH Molecular Libraries Screening Centers Network [MLSCN] NIH Chemical Genomics Center [NCGC] MLSCN Grant: MH081283-01A1 PI Name: Holman, T.R., University of California, Santa Cruz Human lipoxygenase 15hLO-1 is a member of the closely related lipoxygenase family of enzymes which catalyze the site-specific oxidation of arachidonic acid to various hormone precursor molecules and as such is a candidate for drug development in a variety of disease areas, such as cancer and inflammat... aid887.table aid887.tbin
888 28 NIH Molecular Libraries Screening Centers Network [MLSCN] NIH Chemical Genomics Center [NCGC] MLSCN Grant: 1 X01 MH078950-01 PI Name: Dr. James Wells, Dr. Enrique Perez-Paya, Dr.Janice Williams, Dr. Dennis Wolan, Mr. Brandon Butler NCGC Assay Overview: The caspases (Cysteine Aspartyl Protease) comprise a related family of 14 dimeric proteases that are critical mediators of apoptosis (programmed cell death) and inflammation. The caspases represent an important class of drug targets for stroke,... aid888.table aid888.tbin
889 74940 NIH Molecular Libraries Screening Centers Network [MLSCN] NIH Chemical Genomics Center [NCGC] MLSCN Grant: 1 X01 MH078950-01 PI Name: Dr. James Wells, Dr. Enrique Perez-Paya, Dr.Janice Williams, Dr. Dennis Wolan, Mr. Brandon Butler NCGC Assay Overview: The caspases (Cysteine Aspartyl Protease) comprise a related family of 14 dimeric proteases that are critical mediators of apoptosis (programmed cell death) and inflammation. The caspases represent an important class of drug targets for stroke,... aid889.table aid889.tbin
890 51 NIH Molecular Libraries Screening Centers Network [MLSCN] NIH Chemical Genomics Center [NCGC] MLSCN Grant: 1 X01 MH079860-01 Assay Provider: Elisabeth D. Martinez, University of Texas SW Medical Center NCGC Assay Overview: The Locus Derepression assay detects the derepression of a GFP reporter that is stably integrated in a region of the genome of murine c127i mammary cells that is presumably silenced. GFP transcription in this construct is controlled by a CMV promoter, which normally is stron... aid890.table aid890.tbin
891 10320 NIH Molecular Libraries Screening Centers Network [MLSCN] NIH Chemical Genomics Center [NCGC] MLSCN Grant: None. NCGC Assay Overview: The P450 gene superfamily is involved in the metabolism and clearance of xenobiotics. This assay used human CYP2D6 to measure the demethylation of ethylene glycol ester of luciferin 6' methyl ether (Luciferin-ME EGE; luciferin detection buffer) to luciferin. The luciferin is then measured by luminescence after the addition of a luciferase detection reagent. Luci... aid891.table aid891.tbin
892 75028 Assay Overview: NIH Molecular Libraries Screening Centers Network [MLSCN] NIH Chemical Genomics Center [NCGC] MLSCN Grant: MH081227-01 PI Name: Natarajan, Amarnath; University of Texas Inhibitors of the BRCT:pBACH1 interaction should prove useful in studies of BRCA1's tumor suppression role and to potentially sensitive tumors to chemotherapeutic agents. A complex of BRCT (C-terminal portion of BRCA1, MW ~35 kDa, His-tagged) and fluorescently labeled pBACH1 phosphorylated 10 aa peptide fragment ... aid892.table aid892.tbin
893 75028 Assay Overview: NIH Molecular Libraries Screening Centers Network [MLSCN] NIH Chemical Genomics Center [NCGC] Structural Genomics Consortium [SGC] Grant number: None HADH2: Hydroxyacyl-Coenzyme A dehydrogenase, type II, was supplied by researchers at the Structural Genomics Consortium, Oxford University lab (PI Udo Oppermann). The mitochondrial enzyme 17-hydroxysteroid dehydrogenase type 10 (HSD17-10) previously classified as type II hydroxyacyl-CoA dehydrogenase (HADH2) catalyzes the NAD+ depen... aid893.table aid893.tbin
894 150839 Human 15-Hydroxyprostaglandin dehydrogenase (HPGD) catalyzes the inactivation of prostaglandin E2, and plays a major role in cancer biology by antagonizing the oncogenic potential of cyclooxygenase type 2 (COX2). Assays are available, based on absorbance/fluorescence increase of NADH with the substrate 15-OH prostaglandin E2. References 1. Myung SJ, Rerko RM, Yan M, Platzer P, Guda K, Dotson A, Lawrence E, Dannenberg AJ, Lovgren AK, Luo G, Pretlow TP, Newman RA, Willis J, Dawson D, Markowitz ... aid894.table aid894.tbin
895 1279 NIH Molecular Libraries Screening Centers Network [MLSCN] NIH Chemical Genomics Center [NCGC] MLSCN Grant: None NCGC Assay Overview: The TNF alpha/NFkB signaling pathway was assayed in NFkB-bla cells (Invitrogen), a ME-180 cervical carcinoma line containing a stably integrated beta-lactamase reporter gene controlled by a NFkB response element. This reporter is induced by TNF alpha at 10 pM EC50 and inhibited by the proteosome inhibitor MG-132 at approximately 100 nM IC50. The assay was screene... aid895.table aid895.tbin
896 58 NIH Molecular Libraries Screening Centers Network [MLSCN] NIH Chemical Genomics Center [NCGC] MLSCN Grant: 1 X01 MH078950-01 PI Name: Dr. James Wells, Dr. Enrique Perez-Paya, Dr.Janice Williams, Dr. Dennis Wolan, Mr. Brandon Butler NCGC Assay Overview: The caspases (Cysteine Aspartyl Protease) comprise a related family of 14 dimeric proteases that are critical mediators of apoptosis (programmed cell death) and inflammation. The caspases represent an important class of drug targets for stroke,... aid896.table aid896.tbin
897 58 NIH Molecular Libraries Screening Centers Network [MLSCN] NIH Chemical Genomics Center [NCGC] MLSCN Grant: 1 X01 MH078950-01 PI Name: Dr. James Wells, Dr. Enrique Perez-Paya, Dr.Janice Williams, Dr. Dennis Wolan, Mr. Brandon Butler NCGC Assay Overview: The caspases (Cysteine Aspartyl Protease) comprise a related family of 14 dimeric proteases that are critical mediators of apoptosis (programmed cell death) and inflammation. The caspases represent an important class of drug targets for stroke,... aid897.table aid897.tbin
898 139950 Grant number: none. Yersinia pestis is the causal agent of the bubonic plague and, although modern antibiotics are extremely effective against the malady, the plague remains a threat in many areas in the world. Outbreaks of hundreds of cases still occur in Asia, Africa and South America and, in the United States cases are reported sporadically, mainly because of people handling infected animals or by being bitten by infected wild rodent fleas (http://www.cdc.gov). YopH (Yersinia outer protein ... aid898.table aid898.tbin
899 10320 NIH Molecular Libraries Screening Centers Network [MLSCN] NIH Chemical Genomics Center [NCGC] MLSCN Grant: None NCGC Assay Overview: The P450 gene superfamily is involved in the metabolism and clearance of xenobiotics. This assay used human CYP2C19 to measure the hydroxylation of ethylene glycol ester of 6' deoxyluciferin (Luciferin-H EGE; luciferin detection buffer) to luciferin. The luciferin is then measured by luminescence after the addition of a luciferase detection reagent. Luciferin-H E... aid899.table aid899.tbin
900 75028 NIH Molecular Libraries Screening Centers Network [MLSCN] NIH Chemical Genomics Center [NCGC] MLSCN Grant: 1 X01 MH078950-01 PI Name: Dr. James Wells, Dr. Enrique Perez-Paya, Dr.Janice Williams, Dr. Dennis Wolan, Mr. Brandon Butler NCGC Assay Overview: The caspases (Cysteine Aspartyl Protease) comprise a related family of 14 dimeric proteases that are critical mediators of apoptosis (programmed cell death) and inflammation. The caspases represent an important class of drug targets for stroke,... aid900.table aid900.tbin
901 123827 NCGC IMPase Assay Overview Lithium has been widely used for the treatment of bipolar disorder. But lithium has a narrow therapeutic index and it can cause side effects such as thirst, weight gain, tremor, polyuria and memory problems. Although the mechanism for lithium action in treatment of bipolar disorder is still not fully understood, inhibition of inositol monophosphatase (IMPase) and the subsequent depletion of the inositol pool in living cells have been implicated as the primary therapeut... aid901.table aid901.tbin
902 126600 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] MLSCN Grant: 1 X01 MH079844-01 Assay Submitter (PI): Dr. SUN, YI, University of Michigan NCGC Assay Overview: Synthetic Lethal Screen for Compounds to Kill Cancer Cells with p53 Mutation. Tumor suppressor gene p53 serves as a major cellular barrier against tumorigenesis. It has been reported that p53 mutations occurs in half of all tumors, and the other half possess alterations in the p53 pathway th... aid902.table aid902.tbin
903 54550 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] MLSCN Grant: 1 X01 MH079844-01 Assay Submitter (PI): Dr. SUN, YI, University of Michigan NCGC Assay Overview: Synthetic Lethal Screen for Compounds to Kill Cancer Cells with p53 Mutation. Tumor suppressor gene p53 serves as a major cellular barrier against tumorigenesis. It has been reported that p53 mutations occurs in half of all tumors, and the other half possess alterations in the p53 pathway th... aid903.table aid903.tbin
904 54550 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] MLSCN Grant: 1 X01 MH079844-01 Assay Submitter (PI): Dr. SUN, YI, University of Michigan NCGC Assay Overview: Synthetic Lethal Screen for Compounds to Kill Cancer Cells with p53 Mutation. Tumor suppressor gene p53 serves as a major cellular barrier against tumorigenesis. It has been reported that p53 mutations occurs in half of all tumors, and the other half possess alterations in the p53 pathway th... aid904.table aid904.tbin
905 120 NIH Molecular Libraries Screening Centers Network [MLSCN] NIH Chemical Genomics Center [NCGC] National Institutes of Environmental Health Sciences [NIEHS] National Toxicology Program [NTP] MLSCN Grant: 1X01MH079867-01 Assay Provider: Marshall W. Nirenberg, U.S. National Heart, Lung and Blood Institute NCGC Assay Overview: We have developed a 1536-well cell-based assay for quantitative high throughput screening (qHTS) against a number of cell lines to determine in vitro cytotoxicity of small mo... aid905.table aid905.tbin
906 120 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] National Institutes of Environmental Health Sciences [NIEHS] National Toxicology Program [NTP] MLSCN Grant: 1X01MH079867-01 Assay Provider: Marshall W. Nirenberg, U.S. National Heart, Lung and Blood Institute NCGC Assay Overview: We have developed a 1536-well cell-based assay for quantitative high throughput screening (qHTS) against a number of cell lines to determine in vitro cytotoxicity of small mo... aid906.table aid906.tbin
907 120 NIH Molecular Libraries Screening Centers Network [MLSCN] NIH Chemical Genomics Center [NCGC] MLSCN Grant: 1X01MH079867-01 Assay Provider: Marshall W. Nirenberg, U.S. National Heart, Lung and Blood Institute CRE Luciferase Assay Overview Memories persist for different lengths of time, from seconds or minutes to a lifetime. There are two kinds of memory: short-term memory (STM) and long-term memory (LTM). STM lasts for minutes to hours, which maybe mediated by modifications of molecules involve... aid907.table aid907.tbin
908 58 NIH Molecular Libraries Screening Centers Network [MLSCN] NIH Chemical Genomics Center [NCGC] MLSCN Grant: 1 X01 MH078950-01 PI Name: Dr. James Wells, Dr. Enrique Perez-Paya, Dr.Janice Williams, Dr. Dennis Wolan, Mr. Brandon Butler NCGC Assay Overview: The caspases (Cysteine Aspartyl Protease) comprise a related family of 14 dimeric proteases that are critical mediators of apoptosis (programmed cell death) and inflammation. The caspases represent an important class of drug targets for stroke,... aid908.table aid908.tbin
909 28 NIH Molecular Libraries Screening Centers Network [MLSCN] NIH Chemical Genomics Center [NCGC] MLSCN Grant: 1 X01 MH078950-01 PI Name: Dr. James Wells, Dr. Enrique Perez-Paya, Dr.Janice Williams, Dr. Dennis Wolan, Mr. Brandon Butler NCGC Assay Overview: The caspases (Cysteine Aspartyl Protease) comprise a related family of 14 dimeric proteases that are critical mediators of apoptosis (programmed cell death) and inflammation. The caspases represent an important class of drug targets for stroke,... aid909.table aid909.tbin
910 2385 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] MLSCN Grant: 1 X01 MH080684-01 PI Name: Dr. Ryszard Kole NCGC Assay Overview: Modulation of Alternative Splicing as Therapy Abnormal splicing is associated with a number of diseases, including cancer and genetic diseases such as cystic fibrosis, muscular dystrophy and beta-thalassemia. Alteration of disease associated splicing patterns provides a promising target for treatment. Work in the Kole labor... aid910.table aid910.tbin
911 58 NIH Molecular Libraries Screening Centers Network [MLSCN] NIH Chemical Genomics Center [NCGC] MLSCN Grant: 1 X01 MH079825-01 Assay Provider: Jeffrey A. Kuret, Ohio State University NCGC Assay Overview: This assay is a confirmation study for the qHTS Assay for Tau Filament Binding (PubChem AID 596). Tau monomers form filaments in vitro in the presence of arachidonic acid. The dye Thioflavine S (ThS) binds to tau filaments and upon binding, increases in fluorescence several fold. Small molecules... aid911.table aid911.tbin
912 70086 NIH Molecular Libraries Screening Centers Network [MLSCN] NIH Chemical Genomics Center [NCGC] MLSCN Grant: 1 X01 MH082337-01 PI Name: Dr. Thomas Bugge NCGC Assay Overview: Lethal factor (LF, 83 kDa), edema factor (98 kDa) and protective antigen (PA, 83 kDa) are lethal toxins produced by bacillus anthracis, a Gram-positive and spore-forming bacterium responsible for anthrax. While LF and EF contribute the cytotoxic activity of anthrax bacteria, PA is required for internalization of LF an EF. ... aid912.table aid912.tbin
914 11410 NIH Molecular Libraries Screening Centers Network [MLSCN] NIH Chemical Genomics Center [NCGC] MLSCN Grant: None NCGC Assay Overview: Hypoxia-inducible factor-1 (HIF-1) is the major hypoxia-regulated transcription factor that controls cellular responses to low oxygen concentration. HIF-1 is composed of two subunits: hypoxia responsive HIF-1 alpha and constitutively expressed HIF-1 beta, which is also known as aryl hydrocarbon receptor nuclear translocator. During hypoxic conditions, HIF-1 alpha... aid914.table aid914.tbin
915 11410 NIH Molecular Libraries Screening Centers Network [MLSCN] NIH Chemical Genomics Center [NCGC] MLSCN Grant: None NCGC Assay Overview: Hypoxia-inducible factor-1 (HIF-1) is the major hypoxia-regulated transcription factor that controls cellular responses to low oxygen concentration. HIF-1 is composed of two subunits: hypoxia responsive HIF-1 alpha and constitutively expressed HIF-1 beta, which is also known as aryl hydrocarbon receptor nuclear translocator. During hypoxic conditions, HIF-1 alpha... aid915.table aid915.tbin
916 120 NIH Molecular Libraries Screening Centers Network [MLSCN] NIH Chemical Genomics Center [NCGC] MLSCN Grant: 1X01MH079867-01 Assay Provider: Marshall W. Nirenberg, U.S. National Heart, Lung and Blood Institute CRE beta-lactamase Assay Overview Memories persist for different lengths of time, from seconds or minutes to a lifetime. There are two kinds of memory: short-term memory (STM) and long-term memory (LTM). STM lasts for minutes to hours, which maybe mediated by modifications of molecules inv... aid916.table aid916.tbin
917 1277 NIH Molecular Libraries Screening Centers Network [MLSCN] NIH Chemical Genomics Center [NCGC] MLSCN Grant: None NCGC Assay Overview: The thrombopoietin (TPO) signaling pathway was assayed in murine Ba/F3 cells stably transfected with the human TPO receptor and a luciferase reporter gene controlled by the Glycoprotein IIb promoter (provided by Axxam). BaF3 cells are an IL-3-dependent pro B cell line that upon TPO stimulation, undergo cell division and induce the megakaryocyte marker, Glycoprot... aid917.table aid917.tbin
918 1277 NIH Molecular Libraries Screening Centers Network [MLSCN] NIH Chemical Genomics Center [NCGC] MLSCN Grant: None NCGC Assay Overview: The thrombopoietin (TPO) signaling pathway was assayed in murine Ba/F3 cells stably transfected with the human TPO receptor and a luciferase reporter gene controlled by the Glycoprotein IIb promoter (provided by Axxam). BaF3 cells are an IL-3-dependent pro B cell line that upon TPO stimulation, undergo cell division and induce the megakaryocyte marker, Glycoprot... aid918.table aid918.tbin
919 58 NIH Molecular Libraries Screening Centers Network [MLSCN] NIH Chemical Genomics Center [NCGC] MLSCN Grant: 1 X01 MH078950-01 PI Name: Dr. James Wells, Dr. Enrique Perez-Paya, Dr.Janice Williams, Dr. Dennis Wolan, Mr. Brandon Butler NCGC Assay Overview: The caspases (Cysteine Aspartyl Protease) comprise a related family of 14 dimeric proteases that are critical mediators of apoptosis (programmed cell death) and inflammation. The caspases represent an important class of drug targets for stroke,... aid919.table aid919.tbin
920 196012 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute (TSRI) Assay Provider: David Frank Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 1 X01 MH079826-01 Grant Proposal PI: David Frank External Assay ID: STAT1_INH_LUMI_1536_%INH Name: Primary cell-based high throughput screening assay to identify inhibitors of STAT1 Description: The signal transducer and activator of tr... aid920.table aid920.tbin
921 1408 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] National Institutes of Environmental Health Sciences [NIEHS] National Toxicology Program [NTP] MLSCN Grant: None NCGC Assay Overview: We have developed a 1536-well cell-based assay for quantitative high throughput screening (qHTS) against a number of cell lines to determine in vitro cytotoxicity of small molecules. This particular assay uses a human lymphoblastoid cell line (LYMP2-001) purchased from ... aid921.table aid921.tbin
922 120 NIH Molecular Libraries Screening Centers Network [MLSCN] NIH Chemical Genomics Center [NCGC] MLSCN Grant: None NCGC Assay Overview: This assay is a retest of actives identified from the Stat signaling pathway assay (PubChem AID 446). The Stat assay is cell-based and measures Interleukin 6 (IL-6) mediated modulation of a beta-lactamase reporter gene controlled by a STAT inducible element (SIE-bla cells, Invitrogen). IL-6 is a cytokine that signals via the JAK/STAT pathway and is implicated in ... aid922.table aid922.tbin
923 75028 NIH Molecular Libraries Screening Centers Network [MLSCN] NIH Chemical Genomics Center [NCGC] MLSCN Grant: 1 X01 MH078950-01 PI Name: Dr. James Wells, Dr. Enrique Perez-Paya, Dr.Janice Williams, Dr. Dennis Wolan, Mr. Brandon Butler NCGC Assay Overview: The rate of false hits is dramatically reduced by the qHTS approach (Inglese et al, PNAS, 103, 1147 (2006)) as spurious high- or low-response wells are quickly revealed when plotted in the context of concentration response. Occasionally, the ar... aid923.table aid923.tbin
924 125218 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] MLSCN Grant: 1 X01 MH079844-01 Assay Submitter (PI): Dr. SUN, YI, University of Michigan NCGC Assay Overview: Synthetic Lethal Screen for Compounds to Kill Cancer Cells with p53 Mutation. Tumor suppressor gene p53 serves as a major cellular barrier against tumorigenesis. It has been reported that p53 mutations occurs in half of all tumors, and the other half possess alterations in the p53 pathway th... aid924.table aid924.tbin
925 65077 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] MLSCN Grant: 1 X01 MH080684-01 PI Name: Dr. Ryszard Kole NCGC Assay Overview: Modulation of Alternative Splicing as Therapy Abnormal splicing is associated with a number of diseases, including cancer and genetic diseases such as cystic fibrosis, muscular dystrophy and beta-thalassemia. Alteration of disease associated splicing patterns provides a promising target for treatment. Work in the Kole labor... aid925.table aid925.tbin
926 73162 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] MLSCN Grant: 1 X01 MH080680-01 PI Name: Dr. Marvin Gershengorn, NIH NCGC Assay Overview: Thyroid Stimulating Hormone Receptor TSH is an alpha/beta heterodimeric glycoprotein hormone secreted from the anterior pituitary gland which belongs to the glycoprotein hormone family. The actions of TSH are mediated by a seven-transmembrane receptor, which upon TSH binding couples preferentially to the G-alpha ... aid926.table aid926.tbin
927 59724 NIH Molecular Libraries Screening Centers Network [MLSCN] NIH Chemical Genomics Center [NCGC] MLSCN Grant: XO1-MH079852-01 PI Name: Nicholson, Ben. Progenra Inc, Malvern, PA NCGC Assay Overview: Homeostasis of cellular proteins is maintained through a combination of synthesis and degradation. The pathway that accounts for the majority of protein degradation is the ubiquitin-proteasomal pathway. Ubiquitin (Ub) is highly conserved in all cells and the generation of a multi-Ub chain typically tar... aid927.table aid927.tbin
928 1279 NIH Molecular Libraries Screening Centers Network [MLSCN] NIH Chemical Genomics Center [NCGC] MLSCN Grant: None NCGC Assay Overview: The TNF alpha/NFkB signaling pathway was assayed in NFkB-bla cells (Invitrogen), a ME-180 cervical carcinoma line containing a stably integrated beta-lactamase reporter gene controlled by a NFkB response element. This reporter is induced by TNF alpha at 10 pM EC50 and inhibited by the proteosome inhibitor MG-132 at approximately 100 nM IC50. The assay was screene... aid928.table aid928.tbin
929 58 NIH Molecular Libraries Screening Centers Network [MLSCN] NIH Chemical Genomics Center [NCGC] MLSCN Grant: 1 X01 MH078950-01 PI Name: Dr. James Wells, Dr. Enrique Perez-Paya, Dr.Janice Williams, Dr. Dennis Wolan, Mr. Brandon Butler NCGC Assay Overview: The caspases (Cysteine Aspartyl Protease) comprise a related family of 14 dimeric proteases that are critical mediators of apoptosis (programmed cell death) and inflammation. The caspases represent an important class of drug targets for stroke,... aid929.table aid929.tbin
930 2385 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] MLSCN Grant: 1 X01 MH080684-01 PI Name: Dr. Ryszard Kole NCGC Assay Overview: Modulation of Alternative Splicing as Therapy Abnormal splicing is associated with a number of diseases, including cancer and genetic diseases such as cystic fibrosis, muscular dystrophy and beta-thalassemia. Alteration of disease associated splicing patterns provides a promising target for treatment. Work in the Kole labor... aid930.table aid930.tbin
931 22 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] MLSCN Grant: 1 X01 MH080684-01 PI Name: Dr. Ryszard Kole NCGC Assay Overview: Modulation of Alternative Splicing as Therapy Abnormal splicing is associated with a number of diseases, including cancer and genetic diseases such as cystic fibrosis, muscular dystrophy and beta-thalassemia. Alteration of disease associated splicing patterns provides a promising target for treatment. Work in the Kole labor... aid931.table aid931.tbin
932 196012 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute (TSRI) Assay Provider: David Frank Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 1 X01 MH079826-01 Grant Proposal PI: David Frank External Assay ID: STAT1_POT_LUMI_1536_%ACT Name: Primary cell-based high throughput screening assay to measure STAT1 activation Description: The signal transducer and activator of transcript... aid932.table aid932.tbin
933 367 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] MLSCN Grant: 1 X01 MH080680-01 PI Name: Dr. Marvin Gershengorn, NIH NCGC Assay Overview: Confirmation of Thyroid Stimulating Hormone Receptor Agonists TSH is an alpha/beta heterodimeric glycoprotein hormone secreted from the anterior pituitary gland which belongs to the glycoprotein hormone family. The actions of TSH are mediated by a seven-transmembrane receptor, which upon TSH binding couples prefe... aid933.table aid933.tbin
934 120 NIH Molecular Libraries Screening Centers Network [MLSCN] NIH Chemical Genomics Center [NCGC] MLSCN Grant: None NCGC Assay Overview: This assay is a counter screen for the Stat signaling pathway assay (PubChem AID 446). The Stat assay is cell-based and measures Interleukin 6 (IL-6) mediated modulation of a beta-lactamase reporter gene controlled by a STAT inducible element (SIE-bla cells, Invitrogen). IL-6 is a cytokine that signals via the JAK/STAT pathway and is implicated in cancer and infl... aid934.table aid934.tbin
935 120 NIH Molecular Libraries Screening Centers Network [MLSCN] NIH Chemical Genomics Center [NCGC] MLSCN Grant: None NCGC Assay Overview: This assay is a retest of actives identified from the Stat signaling pathway assay (PubChem AID 446). The Stat assay is cell-based and measures Interleukin 6 (IL-6) mediated modulation of a beta-lactamase reporter gene controlled by a STAT inducible element (SIE-bla cells, Invitrogen). IL-6 is a cytokine that signals via the JAK/STAT pathway and is implicated in ... aid935.table aid935.tbin
936 119 List of compounds to be tested: Compounds that met the active criterion of Z-score is </= -3 in the in vitro PLK1-PBD binding primary screen AID 693, will be tested in 10-point concentration response assays in the presence of 1.0 mM DTT. The PLK-1-PBD hit characterization compound concentration dependent redox cycling H2O2 generation assay in the presence of 1.0 mM DTT, has been developed to evaluate actives that were identified in the in vitro PLK1-PBD binding primary screen AID 693, conducte... aid936.table aid936.tbin
937 58 NIH Molecular Libraries Screening Centers Network [MLSCN] NIH Chemical Genomics Center [NCGC] MLSCN Grant: 1 X01 MH079825-01 Assay Provider: Jeffrey A. Kuret, Ohio State University NCGC Assay Overview: Lewy body disease is characterized by the appearance of lesions containing alpha-synuclein. The protein components in these lesions can be induced to form filaments under experimentally tractable times when incubated in vitro with anionic inducers such as alkyl sulfate detergents or fatty acids.... aid937.table aid937.tbin
938 73162 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] MLSCN Grant: 1 X01 MH080680-01 PI Name: Dr. Marvin Gershengorn NCGC Assay Overview: This cell based assay utilized a cyclic nucleotide gated ion channel (CNG) as a biosensor for cAMP induction. HEK 293 cells stably expressing the modified CNG were purchased from BD biosciences (http://www.atto.com/products/actone/features_benefits.shtml) . Stimulation of cAMP production causes the CNG to open and subse... aid938.table aid938.tbin
939 151 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] MLSCN Grant: 1 X01 MH080680-01 PI Name: Dr. Marvin Gershengorn, NIH NCGC Assay Overview: Thyroid Stimulating Hormone Receptor TSH is an alpha/beta heterodimeric glycoprotein hormone secreted from the anterior pituitary gland which belongs to the glycoprotein hormone family. The actions of TSH are mediated by a seven-transmembrane receptor, which upon TSH binding couples preferentially to the G-alpha ... aid939.table aid939.tbin
940 262043 NIH Molecular Libraries Screening Centers Network [MLSCN] Emory Chemical Biology Discovery Center in MLSCN Assay provider: Dr. Raymond Dingledine, Emory University MLSCN Grant: 5-U01NS058158-02 Assay Overview: Injury of the brain is a major cause of death and morbidity after the prolonged seizures termed status epilepticus (SE). Studies in rodents have demonstrated that cyclooxygenase 2 (COX2) activation by ischemia and SE generally contributes to neuronal injury, but multiple downstream COX2 si... aid940.table aid940.tbin
941 349 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute, TSRI Assay Provider: Peter Tobias, TSRI Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 1 X01 MH079857-01 Grant Proposal PI: Peter Tobias External Assay ID: TRL4-MyD88_INH_BLA_1536_3X%INH Name: Confirmatory cell-based high-throughput screening assay for inhibitors of TLR4-MyD88 binding Description: In atherosclerosis, kidn... aid941.table aid941.tbin
942 35 NIH Molecular Libraries Screening Centers Network [MLSCN] NIH Chemical Genomics Center [NCGC] MLSCN Grant: 1 X01 MH082337-01 PI Name: Dr. Thomas Bugge NCGC Assay Overview: Lethal factor (LF, 83 kDa), edema factor (98 kDa) and protective antigen (PA, 83 kDa) are lethal toxins produced by bacillus anthracis, a Gram-positive and spore-forming bacterium responsible for anthrax. While LF and EF contribute the cytotoxic activity of anthrax bacteria, PA is required for internalization of LF an EF. ... aid942.table aid942.tbin
943 1279 NIH Molecular Libraries Screening Centers Network [MLSCN] NIH Chemical Genomics Center [NCGC] MLSCN Grant: None NCGC Assay Overview: Acetylcholine muscarinic M1 receptor is a G-protein coupled receptor (GPCR) coupling to the Gq G protein. It is mainly expressed in brain and functionally related to the learning and memory processes. The agonists of this receptor have the potential for the treatment of Alzheimer's disease and cognitive impairments associated with Schizophrenia. Upon an activatio... aid943.table aid943.tbin
944 1279 NIH Molecular Libraries Screening Centers Network [MLSCN] NIH Chemical Genomics Center [NCGC] MLSCN Grant: None NCGC Assay Overview: Acetylcholine muscarinic M1 receptor is a G-protein coupled receptor (GPCR) coupling to the Gq G protein. It is mainly expressed in brain and functionally related to the learning and memory processes. The agonists of this receptor have the potential for the treatment of Alzheimer's disease and cognitive impairments associated with Schizophrenia. Upon an activat... aid944.table aid944.tbin
945 1279 NIH Molecular Libraries Screening Centers Network [MLSCN] NIH Chemical Genomics Center [NCGC] MLSCN Grant: None PI names: Malcom Walkinshaw, Hugh Morgan and Linda Gilmore University of Edinburgh, Edinburgh, UK Assay Overview: Species of Leishmania are responsible for a wide spectrum of diseases throughout the tropics and subtropics, ranging from self-healing ulcers to highly disfiguring lesions and serious, often lethal visceral diseases, such as kala-azar. In Leishmania spp. it has been shown... aid945.table aid945.tbin
946 1408 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] National Institutes of Environmental Health Sciences [NIEHS] National Toxicology Program [NTP] MLSCN Grant: None NCGC Assay Overview: We have developed a 1536-well cell-based assay for quantitative high throughput screening (qHTS) against a number of cell lines to determine in vitro cytotoxicity of small molecules. This particular assay uses a human lymphoblastoid cell line (LYMP1-001) purchased from ... aid946.table aid946.tbin
947 1408 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] National Institutes of Environmental Health Sciences [NIEHS] National Toxicology Program [NTP] MLSCN Grant: None NCGC Assay Overview: We have developed a 1536-well cell-based assay for quantitative high throughput screening (qHTS) against a number of cell lines to determine in vitro cytotoxicity of small molecules. This particular assay uses a human lymphoblastoid cell line (LYMP1-001) purchased from ... aid947.table aid947.tbin
948 1408 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] National Institutes of Environmental Health Sciences [NIEHS] National Toxicology Program [NTP] MLSCN Grant: None NCGC Assay Overview: We have developed a 1536-well cell-based assay for quantitative high throughput screening (qHTS) against a number of cell lines to determine in vitro cytotoxicity of small molecules. This particular assay uses a human lymphoblastoid cell line (LYMP1-002) purchased from ... aid948.table aid948.tbin
949 443 NIH Molecular Libraries Screening Centers Network [MLSCN] Emory Chemical Biology Discovery Center in MLSCN Assay provider: Dr. Paul De Figueiredo, Texas A&M University System MLSCN Grant: 1 X01 MH079865-01 Assay Overview: Shwachman-Diamond Syndrome (SDS) is an autosomal recessive genetic syndrome that results in hematopoietic defects as well as impaired pancreatic function and skeletal development (1, 2). Presently, therapeutic options are limited to supportive care or bone marrow transplant, ... aid949.table aid949.tbin
950 194920 University of New Mexico Assay Overview: Assay Support: NIH 1X01 MH079850-01 HTS to identify small molecule regulators of Bcl-2 family protein interactions PI: Larry Sklar, Ph.D. Assay Implementatiion: Peter Simons Ph.D, Susan Young MS, Anna Waller Ph.D, Mark Carter MS Assay Background and Significance: One arm of apoptosis is regulated by the balance of anti-apoptotic and pro-apoptotic Bcl-2 family members. In humans, six genes have been identified that encode anti-apoptotic proteins cha... aid950.table aid950.tbin
951 194920 University of New Mexico Assay Overview: Assay Support: NIH 1X01 MH079850-01 HTS to identify small molecule regulators of Bcl-2 family protein interactions PI: Larry Sklar, Ph.D. Assay Implementatiion: Peter Simons Ph.D, Susan Young MS, Anna Waller Ph.D, Mark Carter MS Assay Background and Significance: One arm of apoptosis is regulated by the balance of anti-apoptotic and pro-apoptotic Bcl-2 family members. In humans, six genes have been identified that encode anti-apoptotic proteins ch... aid951.table aid951.tbin
952 194920 University of New Mexico Assay Overview: Assay Support: NIH 1X01 MH079850-01 HTS to identify small molecule regulators of Bcl-2 family protein interactions PI: Larry Sklar, Ph.D. Assay Implementatiion: Peter Simons Ph.D, Susan Young MS, Anna Waller Ph.D, Mark Carter MS Assay Background and Significance: One arm of apoptosis is regulated by the balance of anti-apoptotic and pro-apoptotic Bcl-2 family members. In humans, six genes have been identified that encode anti-apoptotic proteins ch... aid952.table aid952.tbin
953 151 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] MLSCN Grant: 1 X01 MH080680-01 PI Name: Dr. Marvin Gershengorn NCGC Assay Overview: This cell based assay utilized a cyclic nucleotide gated ion channel (CNG) as a biosensor for cAMP induction. HEK 293 cells stably expressing the modified CNG were purchased from BD biosciences (http://www.atto.com/products/actone/features_benefits.shtml) . Stimulation of cAMP production causes the CNG to open and subse... aid953.table aid953.tbin
954 1279 NIH Molecular Libraries Screening Centers Network [MLSCN] NIH Chemical Genomics Center [NCGC] MLSCN Grant: None Wael M. Rabeh, Lyudmila Nedyalkova and Hee-Wan Park [Structural Genomics Consortium, 100 College St. Toronto, Ontario, Canada] NCGC Assay Overview: Human pyruvate kinase muscle 2 enzyme was supplied as a highly purified (>95% pure) preparation by the Structural Genomics Consortium in Toronto (Ontario, Canada) and was assayed for its ability to generate ATP from ADP using phosphoenol... aid954.table aid954.tbin
955 1408 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] National Institutes of Environmental Health Sciences [NIEHS] National Toxicology Program [NTP] MLSCN Grant: None NCGC Assay Overview: We have developed a 1536-well cell-based assay for quantitative high throughput screening (qHTS) against a number of cell lines to determine in vitro cytotoxicity of small molecules. This particular assay uses a human lymphoblastoid cell line (LYMP1-002) purchased from ... aid955.table aid955.tbin
956 58 NIH Molecular Libraries Screening Centers Network [MLSCN] NIH Chemical Genomics Center [NCGC] MLSCN Grant: 1 X01 MH079825-01 Assay Provider: Jeffrey A. Kuret, Ohio State University NCGC Assay Overview: Alzheimer disease is characterized by the accumulation of proteinacious aggregates comprised of tau and beta-amyloid. The protein components of each lesion can be induced to form filaments under experimentally tractable times when incubated in vitro with anionic inducers such as alkyl sulfate d... aid956.table aid956.tbin
957 1279 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] MLSCN Grant: 1 X01MH78932-01 PI Name: Zheng, Wei [NIH] NCGC Assay Overview: Alpha-galactosidase is a homodimeric glycoprotein that hydrolyzes the terminal alpha-galactosyl moieties from glycolipids and glycoproteins. Deficiency of this enzyme results in Fabry Disease with progressive accumulation of globotriaosylceramide and other glycosphingolipids in vascular endothelial cells that causes renal fail... aid957.table aid957.tbin
958 1279 NIH Molecular Libraries Screening Centers Network [MLSCN] NIH Chemical Genomics Center [NCGC] MLSCN Grant: None Wael M. Rabeh, Lyudmila Nedyalkova and Hee-Wan Park [Structural Genomics Consortium, 100 College St. Toronto, Ontario, Canada] NCGC Assay Overview: Human pyruvate kinase muscle 2 enzyme was supplied as a highly purified (>95% pure) preparation by the Structural Genomics Consortium in Toronto (Ontario, Canada) and was assayed for its ability to generate ATP from ADP using phosphoenol... aid958.table aid958.tbin
959 1279 NIH Molecular Libraries Screening Centers Network [MLSCN] NIH Chemical Genomics Center [NCGC] MLSCN Grant: None PI names: Malcom Walkinshaw, Hugh Morgan and Linda Gilmore University of Edinburgh, Edinburgh, UK Assay Overview: Species of Leishmania are responsible for a wide spectrum of diseases throughout the tropics and subtropics, ranging from self-healing ulcers to highly disfiguring lesions and serious, often lethal visceral diseases, such as kala-azar. In Leishmania spp. it has been shown... aid959.table aid959.tbin
960 1408 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] National Institutes of Environmental Health Sciences [NIEHS] National Toxicology Program [NTP] MLSCN Grant: None NCGC Assay Overview: We have developed a 1536-well cell-based assay for quantitative high throughput screening (qHTS) against a number of cell lines to determine in vitro cytotoxicity of small molecules. This particular assay uses a human lymphoblastoid cell line (LYMP1-002) purchased from ... aid960.table aid960.tbin
961 1408 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] National Institutes of Environmental Health Sciences [NIEHS] National Toxicology Program [NTP] MLSCN Grant: None NCGC Assay Overview: We have developed a 1536-well cell-based assay for quantitative high throughput screening (qHTS) against a number of cell lines to determine in vitro cytotoxicity of small molecules. This particular assay uses a human lymphoblastoid cell line (LYMP1-003) purchased from ... aid961.table aid961.tbin
962 1408 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] National Institutes of Environmental Health Sciences [NIEHS] National Toxicology Program [NTP] MLSCN Grant: None NCGC Assay Overview: We have developed a 1536-well cell-based assay for quantitative high throughput screening (qHTS) against a number of cell lines to determine in vitro cytotoxicity of small molecules. This particular assay uses a human lymphoblastoid cell line (LYMP1-003) purchased from ... aid962.table aid962.tbin
963 1408 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] National Institutes of Environmental Health Sciences [NIEHS] National Toxicology Program [NTP] MLSCN Grant: None NCGC Assay Overview: We have developed a 1536-well cell-based assay for quantitative high throughput screening (qHTS) against a number of cell lines to determine in vitro cytotoxicity of small molecules. This particular assay uses a human lymphoblastoid cell line (LYMP1-003) purchased from ... aid963.table aid963.tbin
964 1408 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] National Institutes of Environmental Health Sciences [NIEHS] National Toxicology Program [NTP] MLSCN Grant: None NCGC Assay Overview: We have developed a 1536-well cell-based assay for quantitative high throughput screening (qHTS) against a number of cell lines to determine in vitro cytotoxicity of small molecules. This particular assay uses a human lymphoblastoid cell line (LYMP1-003) purchased from ... aid964.table aid964.tbin
965 1408 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] National Institutes of Environmental Health Sciences [NIEHS] National Toxicology Program [NTP] MLSCN Grant: None NCGC Assay Overview: We have developed a 1536-well cell-based assay for quantitative high throughput screening (qHTS) against a number of cell lines to determine in vitro cytotoxicity of small molecules. This particular assay uses a human lymphoblastoid cell line (LYMP2-003) purchased from ... aid965.table aid965.tbin
966 1408 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] National Institutes of Environmental Health Sciences [NIEHS] National Toxicology Program [NTP] MLSCN Grant: None NCGC Assay Overview: We have developed a 1536-well cell-based assay for quantitative high throughput screening (qHTS) against a number of cell lines to determine in vitro cytotoxicity of small molecules. This particular assay uses a human lymphoblastoid cell line (LYMP2-004) purchased from ... aid966.table aid966.tbin
967 1408 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] National Institutes of Environmental Health Sciences [NIEHS] National Toxicology Program [NTP] MLSCN Grant: None NCGC Assay Overview: We have developed a 1536-well cell-based assay for quantitative high throughput screening (qHTS) against a number of cell lines to determine in vitro cytotoxicity of small molecules. This particular assay uses a human lymphoblastoid cell line (LYMP2-005) purchased from ... aid967.table aid967.tbin
968 1408 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] National Institutes of Environmental Health Sciences [NIEHS] National Toxicology Program [NTP] MLSCN Grant: None NCGC Assay Overview: We have developed a 1536-well cell-based assay for quantitative high throughput screening (qHTS) against a number of cell lines to determine in vitro cytotoxicity of small molecules. This particular assay uses a human lymphoblastoid cell line (LYMP2-009) purchased from ... aid968.table aid968.tbin
969 1408 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] National Institutes of Environmental Health Sciences [NIEHS] National Toxicology Program [NTP] MLSCN Grant: None NCGC Assay Overview: We have developed a 1536-well cell-based assay for quantitative high throughput screening (qHTS) against a number of cell lines to determine in vitro cytotoxicity of small molecules. This particular assay uses a human lymphoblastoid cell line (LYMP2-011) purchased from ... aid969.table aid969.tbin
970 1408 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] National Institutes of Environmental Health Sciences [NIEHS] National Toxicology Program [NTP] MLSCN Grant: None NCGC Assay Overview: We have developed a 1536-well cell-based assay for quantitative high throughput screening (qHTS) against a number of cell lines to determine in vitro cytotoxicity of small molecules. This particular assay uses a human lymphoblastoid cell line (LYMP2-013) purchased from ... aid970.table aid970.tbin
971 1408 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] National Institutes of Environmental Health Sciences [NIEHS] National Toxicology Program [NTP] MLSCN Grant: None NCGC Assay Overview: We have developed a 1536-well cell-based assay for quantitative high throughput screening (qHTS) against a number of cell lines to determine in vitro cytotoxicity of small molecules. This particular assay uses a human lymphoblastoid cell line (LYMP2-015) purchased from ... aid971.table aid971.tbin
972 1408 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] National Institutes of Environmental Health Sciences [NIEHS] National Toxicology Program [NTP] MLSCN Grant: None NCGC Assay Overview: We have developed a 1536-well cell-based assay for quantitative high throughput screening (qHTS) against a number of cell lines to determine in vitro cytotoxicity of small molecules. This particular assay uses a human lymphoblastoid cell line (LYMP2-017) purchased from ... aid972.table aid972.tbin
973 1408 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] National Institutes of Environmental Health Sciences [NIEHS] National Toxicology Program [NTP] MLSCN Grant: None NCGC Assay Overview: We have developed a 1536-well cell-based assay for quantitative high throughput screening (qHTS) against a number of cell lines to determine in vitro cytotoxicity of small molecules. This particular assay uses a human lymphoblastoid cell line (LYMP2-019) purchased from ... aid973.table aid973.tbin
974 1408 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] National Institutes of Environmental Health Sciences [NIEHS] National Toxicology Program [NTP] MLSCN Grant: None NCGC Assay Overview: We have developed a 1536-well cell-based assay for quantitative high throughput screening (qHTS) against a number of cell lines to determine in vitro cytotoxicity of small molecules. This particular assay uses a human lymphoblastoid cell line (LYMP2-021) purchased from ... aid974.table aid974.tbin
975 1408 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] National Institutes of Environmental Health Sciences [NIEHS] National Toxicology Program [NTP] MLSCN Grant: None NCGC Assay Overview: We have developed a 1536-well cell-based assay for quantitative high throughput screening (qHTS) against a number of cell lines to determine in vitro cytotoxicity of small molecules. This particular assay uses a human lymphoblastoid cell line (LYMP2-023) purchased from ... aid975.table aid975.tbin
976 1408 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] National Institutes of Environmental Health Sciences [NIEHS] National Toxicology Program [NTP] MLSCN Grant: None NCGC Assay Overview: We have developed a 1536-well cell-based assay for quantitative high throughput screening (qHTS) against a number of cell lines to determine in vitro cytotoxicity of small molecules. This particular assay uses a human lymphoblastoid cell line (LYMP2-025) purchased from ... aid976.table aid976.tbin
977 1408 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] National Institutes of Environmental Health Sciences [NIEHS] National Toxicology Program [NTP] MLSCN Grant: None NCGC Assay Overview: We have developed a 1536-well cell-based assay for quantitative high throughput screening (qHTS) against a number of cell lines to determine in vitro cytotoxicity of small molecules. This particular assay uses a human lymphoblastoid cell line (LYMP2-002) purchased from ... aid977.table aid977.tbin
978 1408 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] National Institutes of Environmental Health Sciences [NIEHS] National Toxicology Program [NTP] MLSCN Grant: None NCGC Assay Overview: We have developed a 1536-well cell-based assay for quantitative high throughput screening (qHTS) against a number of cell lines to determine in vitro cytotoxicity of small molecules. This particular assay uses a human lymphoblastoid cell line (LYMP2-006) purchased from ... aid978.table aid978.tbin
979 1408 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] National Institutes of Environmental Health Sciences [NIEHS] National Toxicology Program [NTP] MLSCN Grant: None NCGC Assay Overview: We have developed a 1536-well cell-based assay for quantitative high throughput screening (qHTS) against a number of cell lines to determine in vitro cytotoxicity of small molecules. This particular assay uses a human lymphoblastoid cell line (LYMP2-007) purchased from ... aid979.table aid979.tbin
980 1408 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] National Institutes of Environmental Health Sciences [NIEHS] National Toxicology Program [NTP] MLSCN Grant: None NCGC Assay Overview: We have developed a 1536-well cell-based assay for quantitative high throughput screening (qHTS) against a number of cell lines to determine in vitro cytotoxicity of small molecules. This particular assay uses a human lymphoblastoid cell line (LYMP2-008) purchased from ... aid980.table aid980.tbin
981 1408 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] National Institutes of Environmental Health Sciences [NIEHS] National Toxicology Program [NTP] MLSCN Grant: None NCGC Assay Overview: We have developed a 1536-well cell-based assay for quantitative high throughput screening (qHTS) against a number of cell lines to determine in vitro cytotoxicity of small molecules. This particular assay uses a human lymphoblastoid cell line (LYMP2-010) purchased from ... aid981.table aid981.tbin
982 1408 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] National Institutes of Environmental Health Sciences [NIEHS] National Toxicology Program [NTP] MLSCN Grant: None NCGC Assay Overview: We have developed a 1536-well cell-based assay for quantitative high throughput screening (qHTS) against a number of cell lines to determine in vitro cytotoxicity of small molecules. This particular assay uses a human lymphoblastoid cell line (LYMP2-012) purchased from ... aid982.table aid982.tbin
983 1408 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] National Institutes of Environmental Health Sciences [NIEHS] National Toxicology Program [NTP] MLSCN Grant: None NCGC Assay Overview: We have developed a 1536-well cell-based assay for quantitative high throughput screening (qHTS) against a number of cell lines to determine in vitro cytotoxicity of small molecules. This particular assay uses a human lymphoblastoid cell line (LYMP2-014) purchased from ... aid983.table aid983.tbin
984 1408 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] National Institutes of Environmental Health Sciences [NIEHS] National Toxicology Program [NTP] MLSCN Grant: None NCGC Assay Overview: We have developed a 1536-well cell-based assay for quantitative high throughput screening (qHTS) against a number of cell lines to determine in vitro cytotoxicity of small molecules. This particular assay uses a human lymphoblastoid cell line (LYMP2-016) purchased from ... aid984.table aid984.tbin
985 1408 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] National Institutes of Environmental Health Sciences [NIEHS] National Toxicology Program [NTP] MLSCN Grant: None NCGC Assay Overview: We have developed a 1536-well cell-based assay for quantitative high throughput screening (qHTS) against a number of cell lines to determine in vitro cytotoxicity of small molecules. This particular assay uses a human lymphoblastoid cell line (LYMP2-018) purchased from ... aid985.table aid985.tbin
986 1408 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] National Institutes of Environmental Health Sciences [NIEHS] National Toxicology Program [NTP] MLSCN Grant: None NCGC Assay Overview: We have developed a 1536-well cell-based assay for quantitative high throughput screening (qHTS) against a number of cell lines to determine in vitro cytotoxicity of small molecules. This particular assay uses a human lymphoblastoid cell line (LYMP2-020) purchased from ... aid986.table aid986.tbin
987 1408 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] National Institutes of Environmental Health Sciences [NIEHS] National Toxicology Program [NTP] MLSCN Grant: None NCGC Assay Overview: We have developed a 1536-well cell-based assay for quantitative high throughput screening (qHTS) against a number of cell lines to determine in vitro cytotoxicity of small molecules. This particular assay uses a human lymphoblastoid cell line (LYMP2-022) purchased from ... aid987.table aid987.tbin
988 1408 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] National Institutes of Environmental Health Sciences [NIEHS] National Toxicology Program [NTP] MLSCN Grant: None NCGC Assay Overview: We have developed a 1536-well cell-based assay for quantitative high throughput screening (qHTS) against a number of cell lines to determine in vitro cytotoxicity of small molecules. This particular assay uses a human lymphoblastoid cell line (LYMP2-024) purchased from ... aid988.table aid988.tbin
989 1408 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] National Institutes of Environmental Health Sciences [NIEHS] National Toxicology Program [NTP] MLSCN Grant: None NCGC Assay Overview: We have developed a 1536-well cell-based assay for quantitative high throughput screening (qHTS) against a number of cell lines to determine in vitro cytotoxicity of small molecules. This particular assay uses a human lymphoblastoid cell line (LYMP2-026) purchased from ... aid989.table aid989.tbin
990 51 NIH Molecular Libraries Screening Centers Network [MLSCN] NIH Chemical Genomics Center [NCGC] MLSCN Grant: 1 X01 MH079860-01 Assay Provider: Elisabeth D. Martinez, University of Texas SW Medical Center NCGC Assay Overview: This assay is a counter screen for the Locus Derepression (LDR) assay (PubChem AID 597). The LDR assay detects the derepression of a GFP reporter that is stably integrated in a region of the genome of murine c127i mammary cells that is presumably silenced. Compounds that cau... aid990.table aid990.tbin
991 6 NIH Molecular Libraries Screening Centers Network [MLSCN] NIH Chemical Genomics Center [NCGC] MLSCN Grant: 1 X01 MH079825-01 Assay Provider: Jeffrey A. Kuret, Ohio State University NCGC Assay Overview: A number of solution-based assays are available for quantifying the amount of aggregated protein, such as fluorescence spectroscopy, sedimentation, and static and laser light scattering. However, none of these methods allows direct visualization of filaments. Transmission electron microscopy (T... aid991.table aid991.tbin
992 1284 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] MLSCN Grant: 1 X01MH78932-01 PI Name: Zheng, Wei [NIH] NCGC Assay Overview: Alpha-galactosidase is a homodimeric glycoprotein that hydrolyzes the terminal alpha-galactosyl moieties from glycolipids and glycoproteins. Deficiency of this enzyme results in Fabry Disease with progressive accumulation of globotriaosylceramide and other glycosphingolipids in vascular endothelial cells that causes renal fail... aid992.table aid992.tbin
993 1408 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] National Institutes of Environmental Health Sciences [NIEHS] National Toxicology Program [NTP] MLSCN Grant: None NCGC Assay Overview: We have developed a 1536-well cell-based assay for quantitative high throughput screening (qHTS) against a number of cell lines to determine in vitro cytotoxicity of small molecules. This particular assay uses a human lymphoblastoid cell line (LYMP1-002) purchased from ... aid993.table aid993.tbin
994 1408 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] National Institutes of Environmental Health Sciences [NIEHS] National Toxicology Program [NTP] MLSCN Grant: None NCGC Assay Overview: We have developed a 1536-well cell-based assay for quantitative high throughput screening (qHTS) against a number of cell lines to determine in vitro cytotoxicity of small molecules. This particular assay uses a human lymphoblastoid cell line (LYMP1-002) purchased from ... aid994.table aid994.tbin
995 72004 qHTS Assay for Inhibitors of the ERK Signaling Pathway using a Homogeneous Screening Assay NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] MLSCN Grant: 1X01MH082406-01 Assay Submitter (PI): Dr. Wei Zheng, NCGC/NIH NCGC Assay Overview: The Ras/extracellular-signal-regulated kinase (ERK) mitogen activated protein (MAP) kinase signaling pathway (ERK1/2 cascade) plays is a key role in transmitting signals from the cell surface to the nucleus (Nis... aid995.table aid995.tbin
996 58 NIH Molecular Libraries Screening Centers Network [MLSCN] NIH Chemical Genomics Center [NCGC] MLSCN Grant: 1 X01 MH078950-01 PI Name: Dr. James Wells, Dr. Enrique Perez-Paya, Dr.Janice Williams, Dr. Dennis Wolan, Mr. Brandon Butler NCGC Assay Overview: The caspases (Cysteine Aspartyl Protease) comprise a related family of 14 dimeric proteases that are critical mediators of apoptosis (programmed cell death) and inflammation. The caspases represent an important class of drug targets for stroke,... aid996.table aid996.tbin
997 1284 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] MLSCN Grant: 1 X01MH78932-01 PI Name: Zheng, Wei [NIH] NCGC Assay Overview: Alpha-glucosidase is responsible for hydrolysis of terminal, non-reducing 1,4-linked alpha-D-glucose residues with release of alpha-D-glucose. It is a lysosomal hydrolase that is required for the degradation of a small percentage (1-3%) of cellular glycogen in human. Deficiency of this enzyme results in glycogen-storage diseas... aid997.table aid997.tbin
998 1279 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] MLSCN Grant: 1 X01MH78932-01 PI Name: Zheng, Wei [NIH] NCGC Assay Overview: Alpha-galactosidase is a homodimeric glycoprotein that hydrolyzes the terminal alpha-galactosyl moieties from glycolipids and glycoproteins. Deficiency of this enzyme results in Fabry Disease with progressive accumulation of globotriaosylceramide and other glycosphingolipids in vascular endothelial cells that causes renal fail... aid998.table aid998.tbin
999 156 Southern Research Molecular Libraries Screening Center (SRMLSC) Southern Research Institute (Birmingham, Alabama) NIH Molecular Libraries Screening Centers Network (MLSCN) Submitted by Dr. Todd Waldman (Lombardi Cancer Center, Georgetown University School of Medicine) Award: 1R03NS050857-01 Numerous genes have been identified which participate, either through activation (oncogenes) or inactivation (tumor suppressors), in the multifactorial process of carcinogenesis (Vogelstein B and Kinzler KW, ... aid999.table aid999.tbin
1000 57 Southern Research Molecular Libraries Screening Center (SRMLSC) Southern Research Institute (Birmingham, Alabama) NIH Molecular Libraries Screening Centers Network (MLSCN) Assay Provider: Dr. Thomas S. Leyh, Albert Einstein College of medicine of Yeshiva University Award: R03 MH078936-01 Streptococcus pneumonia (SP) takes the lives of nearly 4,000 people daily and antibiotic resistant strains are becoming an increasing problem. Because of this, the discovery of drugs targeting novel pathways... aid1000.table aid1000.tbin
1001 195632 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Proposal Number: 1R03 MH082385-01 Alkaline phosphatases (EC 3.1.3.1) (APs) catalyze the hydrolysis of phosphomonoesters, releasing phosphate and alcohol. APs are dimeric enzymes found in the most organisms. In human, four isozymes of APs have been identified. Three isozymes ... aid1001.table aid1001.tbin
1002 9 NIH Molecular Libraries Screening Centers Network [MLSCN] NIH Chemical Genomics Center [NCGC] MLSCN Grant: MH079825-01 Assay Provider: Shoichet, Brian K. This aggregation profiling approach exploits the sensitivity of aggregate formation to detergent. Inhibition of b-lactamase is measured in the presence and absence of 0.01% Triton X-100 (Feng 2007). This particular assay is a confirmation of previous qHTS (Inglese, 2006), Pubchem AID 584, assay with presence of 0.01% Triton X-100. For a relate... aid1002.table aid1002.tbin
1003 8 NIH Molecular Libraries Screening Centers Network [MLSCN] NIH Chemical Genomics Center [NCGC] MLSCN Grant: MH079825-01 Assay Provider: Shoichet, Brian K. This aggregation profiling approach exploits the sensitivity of aggregate formation to detergent. Inhibition of b-lactamase is measured in the presence and absence of 0.01% Triton X-100 (Feng 2007). This particular assay is a confirmation of previous qHTS (Inglese, 2006), Pubchem AID 584, assay with presence of 0.01% Triton X-100. For a relate... aid1003.table aid1003.tbin
1004 156 Southern Research Molecular Libraries Screening Center (SRMLSC) Southern Research Institute (Birmingham, Alabama) NIH Molecular Libraries Screening Centers Network (MLSCN) Submitted by Dr. Todd Waldman (Lombardi Cancer Center, Georgetown University School of Medicine) Award: R03-NS053741 Numerous genes have been identified which participate, either through activation (oncogenes) or inactivation (tumor suppressors), in the multifactorial process of carcinogenesis (Vogelstein B and Kinzler KW, 200... aid1004.table aid1004.tbin
1006 195634 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Number: None This functional assay was developed for detection of compounds inhibiting luciferase. These compounds would be observed as false positives of assays employing luciferase-based detection. aid1006.table aid1006.tbin
1007 194920 University of New Mexico Assay Overview: Assay Support: NIH 1X01 MH079850-01 HTS to identify small molecule regulators of Bcl-2 family protein interactions PI: Larry Sklar, Ph.D. Assay Implementatiion: Peter Simons Ph.D, Susan Young MS, Anna Waller Ph.D, Mark Carter MS Assay Background and Significance: One arm of apoptosis is regulated by the balance of anti-apoptotic and pro-apoptotic Bcl-2 family members. In humans, six genes have been identified that encode anti-apoptotic proteins ch... aid1007.table aid1007.tbin
1008 194920 University of New Mexico Assay Overview: Assay Support: NIH 1X01 MH079850-01 HTS to identify small molecule regulators of Bcl-2 family protein interactions PI: Larry Sklar, Ph.D. Assay Implementatiion: Peter Simons Ph.D, Susan Young MS, Anna Waller Ph.D, Mark Carter MS Assay Background and Significance: One arm of apoptosis is regulated by the balance of anti-apoptotic and pro-apoptotic Bcl-2 family members. In humans, six genes have been identified that encode anti-apoptotic proteins ch... aid1008.table aid1008.tbin
1009 194920 University of New Mexico Assay Overview: Assay Support: NIH 1X01 MH079850-01 HTS to identify small molecule regulators of Bcl-2 family protein interactions PI: Larry Sklar, Ph.D. Assay Implementatiion: Peter Simons Ph.D, Susan Young MS, Anna Waller Ph.D, Mark Carter MS Assay Background and Significance: One arm of apoptosis is regulated by the balance of anti-apoptotic and pro-apoptotic Bcl-2 family members. In humans, six genes have been identified that encode anti-apoptotic proteins ch... aid1009.table aid1009.tbin
1010 48000 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Number: 1R03 DA024887-01 Assay Provider: Dr. John C. Reed, Sanford-Burnham Medical Research Institute The endoplasmic reticulum (ER) fulfills multiple cellular functions (reviewed in [1-4]). The lumen of the ER contains high concentration of Ca2+ due to the transport of cal... aid1010.table aid1010.tbin
1011 27 NIH Molecular Libraries Screening Centers Network [MLSCN] NIH Chemical Genomics Center [NCGC] MLSCN Grant: MH076449-01 Assay Provider: Prof David Williams, Illinois State University This is a confirmation assay for PubChem BioAssay AID 448. Schistosoma mansoni, a causative agent of schistosomiasis, resides in the bloodstream of their host up to 30 years without being eliminated by the host immune attack. One proposed survival mechanism is the production of an antioxidant "firewall" that neutral... aid1011.table aid1011.tbin
1012 195634 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Proposal Number: MH077602-01 Alkaline phosphatase (EC 3.1.3.1) (APs) catalyze the hydrolysis of phosphomonoesters, releasing phosphate and alcohol. APs are dimeric enzymes found in the most organism. In human, four isozymes of APs have been identified. Three isozymes are tis... aid1012.table aid1012.tbin
1013 493 Data Source: Columbia University Molecular Screening Center Source (MLSCN Center Name): Columbia University Molecular Screening Center Center Affiliation: Columbia University Molecular Screening Center Assay Provider: Thomas Mayer, Columbia University Molecular Screening Center Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 1R03 MH076343-01 Cytokines such as TNF alpha and IL-1B activate a pro-inflammatory response in endothelial cells by nuclear translocation... aid1013.table aid1013.tbin
1014 240 Principal Investigator: Hattie D. Gresham, Ph.D. Grant: NIH 1X01MH078952-01 Screening Center: New Mexico Molecular Libraries Screening Center Assay Background and Significance Quorum sensing is a cell-to-cell communication system that permits members of a bacterial population to coordinate their behavior dependent on cell density (for review see Waters and Bassler, 2005). The mediators of this communication system are small, diffusible pheromones or autoinducers that are secreted by the bacte... aid1014.table aid1014.tbin
1015 32 Principal Investigator: Hattie D. Gresham, Ph.D. Grant: NIH 1X01MH078952-01 Screening Center: New Mexico Molecular Libraries Screening Center Assay Background and Significance Quorum sensing is a cell-to-cell communication system that permits members of a bacterial population to coordinate their behavior dependent on cell density (for review see Waters and Bassler, 2005). The mediators of this communication system are small, diffusible pheromones or autoinducers that are secreted by the bacte... aid1015.table aid1015.tbin
1016 195645 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Proposal Number: MH082385-01 Alkaline phosphatase (EC 3.1.3.1) (APs) catalyze the hydrolysis of phosphomonoesters, releasing inorganic phosphate and alcohol. APs are dimeric enzymes found in most organisms. In human, four isozymes of APs have been identified. One isozyme is ... aid1016.table aid1016.tbin
1017 67 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Proposal Number: XO1 MH077602-01 Alkaline phosphatase (EC 3.1.3.1) (APs) catalyze the hydrolysis of phosphomonoesters, releasing inorganic phosphate and alcohol. APs are dimeric enzymes found in most organisms. In human, four isozymes of APs have been identified. One isozym... aid1017.table aid1017.tbin
1018 195645 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Proposal Number: MH081277-01 Assay Provider: John C. Reed, Sanford-Burnham Medical Research Institute, San Diego, CA Apoptosis plays an essential role in many aspects of normal development and physiology, becoming dysregulated in myriad diseases characterized by insufficient... aid1018.table aid1018.tbin
1019 194182 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Proposal Number: MH077602-01 Alkaline phosphatase (EC 3.1.3.1) (APs) catalyze the hydrolysis of phosphomonoesters, releasing inorganic phosphate and alcohol. APs are dimeric enzymes found in most organisms. In human, four isozymes of APs have been identified. One isozyme is... aid1019.table aid1019.tbin
1020 195634 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) This functional assay was developed for detection of compounds inhibiting glucose-6-phosphate dehydrogenase (G6PDH) from Leuconostoc mesenteroides. These compounds would be observed as false positives of assays employing G6PDH, e.g. cytochrome P450 enzyme assays where G6PDH is uti... aid1020.table aid1020.tbin
1021 218788 NIH Molecular Libraries Screening Centers Network [MLSCN] Emory Chemical Biology Discovery Center in MLSCN Assay provider: Nikolovska-Coleska, University of Michigan MLSCN Grant: R21NS057014 The Bcl-2 protein family includes anti-apoptotic proteins such as Bcl-2, Bcl-xL and Mcl-1 and pro-apoptotic proteins such as Bak, Bax, Bim, Bid and Bad. All members of the Bcl-2 protein family contain at least one conserved Bcl-2 homology (BH) domain. These domains have been demonstrated to be involved in th... aid1021.table aid1021.tbin
1022 217515 NIH Molecular Libraries Screening Centers Network [MLSCN] Emory Chemical Biology Discovery Center in MLSCN Assay provider: Nikolovska-Coleska, University of Michigan MLSCN Grant: R21NS057014 The Bcl-2 protein family includes anti-apoptotic proteins such as Bcl-2, Bcl-xL and Mcl-1 and pro-apoptotic proteins such as Bak, Bax, Bim, Bid and Bad. All members of the Bcl-2 protein family contain at least one conserved Bcl-2 homology (BH) domain. These domains have been demonstrated to be involved in th... aid1022.table aid1022.tbin
1023 6 Data Source: Columbia University Molecular Screening Center Source (MLSCN Center Name): Columbia University Molecular Screening Center Center Affiliation: Columbia University Molecular Screening Center Assay Provider: Thomas Mayer, Columbia University Molecular Screening Center Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 1R03MH076344-01 Eight compounds were confirmed as hits from the original TNFalpha induced NFkappaB translocation assay (primary AID: 438, ... aid1023.table aid1023.tbin
1024 95868 Sanford-Burnham Center for Chemical Genomics (SBCCG) Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Number: None The cytochrome P450 enzymes represent a diverse superfamily of hemoproteins present in eukaryotic, bacterial, and archaean systems. The primary function of these enzymes is in the metabolism and clearance of both endogenous and exogenous (xenobiotic) compounds due to their propensity to metabolize mult... aid1024.table aid1024.tbin
1025 95867 Sanford-Burnham Center for Chemical Genomics (SBCCG) Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Number: None The cytochrome P450 enzymes represent a diverse superfamily of hemoproteins present in eukaryotic, bacterial, and archaean systems. The primary function of these enzymes is in the metabolism and clearance of both endogenous and exogenous (xenobiotic) compounds due to their propensity to metabolize mult... aid1025.table aid1025.tbin
1026 301 Data Source: Columbia University Molecular Screening Center Source (MLSCN Center Name): Columbia University Molecular Screening Center Center Affiliation: Columbia University Molecular Screening Center Assay Provider: Dr. Bruce D. Hammock, UC, Davis, CA. Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: X01 MH078954-01 Hypertension and vascular inflammation are associated with cardiovascular diseases, the primary cause of death in our society. Because a large pro... aid1026.table aid1026.tbin
1027 195634 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Number: None This functional assay was developed for detection of compounds activating luciferase. These compounds would be observed as false positives of assays with increase-of-signal detection employing luciferase-based reactions. Potentially, the same compounds would act... aid1027.table aid1027.tbin
1028 76 Southern Research Molecular Libraries Screening Center (SRMLSC) Southern Research Institute (Birmingham, Alabama) NIH Molecular Libraries Screening Centers Network (MLSCN) Assay Provider: Dr. Thomas S. Leyh, Albert Einstein College of Medicine of Yeshiva University Award: R03 MH078936-01 Streptococcus pneumoniae takes the lives of nearly 4,000 people daily and antibiotic resistant strains are becoming an increasing problem. Because of this, the discovery of drugs targeting novel pathways ha... aid1028.table aid1028.tbin
1029 109291 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Proposal Number: 1R03 MH076502-01 Assay Provider: Dr. Fabienne Paumet, Columbia University Phagocytic uptake of large particles such as invading pathogens, foreign particles and dead cell bodies represents a key component of the immune system of mammalian organisms. Due to ... aid1029.table aid1029.tbin
1030 220402 Aldehyde dehydrogenase 1 (ALDH1A1) catalyzes the NAD+ dependent oxidation of a variety of endogenous and exogenous aldehydes to the corresponding carboxylic acids. The enzyme is the critical step in the metabolic activation of retinoic acid, which plays essential roles as nuclear receptor ligand. Furthermore, the precursor, retinaldehyde has recently been shown to play a fundamental role in adipogenesis and obesity, which makes inhibitor development a possible target in metabolic diseases. See [1... aid1030.table aid1030.tbin
1031 102 Data Source: Columbia University Molecular Screening Center Source (MLSCN Center Name): Columbia University Molecular Screening Center Center Affiliation: Columbia University Molecular Screening Center Assay Provider: Drs. Tomas Mustelin and Lutz Tautz, Burnham Institute for Medical Research, La Jolla, CA Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: X01-MH077604-01 LYP, a lymphocyte specific protein tyrosine phosphatase that plays a critical regulatory role ... aid1031.table aid1031.tbin
1032 196255 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute (TSRI) Assay Provider: Pat Griffin, TSRI Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 1 X01 MH079861-01 Grant Proposal PI: Pat Griffin, TSRI External Assay ID: PPARgSRC2_AG_TRFRET_1536_%ACT Name: Pimary biochemical High Throughput Screening assay for agonists of the steroid receptor coactivator 2 (SRC-2) recruitment by the... aid1032.table aid1032.tbin
1033 3684 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Number: XO1 MH078942 Assay Provider: Dr. Maurizio Pellecchia, Sanford-Burnham Medical Research Institute The misregulation of protein folding often results in a variety of deleterious consequences on cellular function that range from the accumulation of protein aggregates l... aid1033.table aid1033.tbin
1034 227 Data Source: Columbia University Molecular Screening Center Source (MLSCN Center Name): Columbia University Molecular Screening Center Center Affiliation: Columbia University Molecular Screening Center Assay Provider: Thomas Mayer, Columbia University Molecular Screening Center Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 1R03 MH076343-01 Cytokines such as TNF alpha and IL-1B activate a pro-inflammatory response in endothelial cells by nuclear translocation ... aid1034.table aid1034.tbin
1035 14 NIH Molecular Libraries Screening Centers Network [MLSCN] NIH Chemical Genomics Center [NCGC] MLSCN Grant: 1 X01 MH079860-01 Assay Provider: Elisabeth D. Martinez, University of Texas SW Medical Center NCGC Assay Overview: This secondary assay characterizes selected actives identified in the qHTS of the Locus Derepression (LDR) assay (PubChem AID 597). The LDR assay detects the derepression of a GFP reporter that is stably integrated in a region of the genome of murine c127i mammary cells that... aid1035.table aid1035.tbin
1036 9 NIH Molecular Libraries Screening Centers Network [MLSCN] NIH Chemical Genomics Center [NCGC] MLSCN Grant: 1 X01 MH079860-01 Assay Provider: Elisabeth D. Martinez, University of Texas SW Medical Center NCGC Assay Overview: This secondary assay characterizes selected actives identified in the qHTS of the Locus Derepression (LDR) assay (PubChem AID 597). The LDR assay detects the derepression of a GFP reporter that is stably integrated in a region of the genome of murine c127i mammary cells that... aid1036.table aid1036.tbin
1037 9 NIH Molecular Libraries Screening Centers Network [MLSCN] NIH Chemical Genomics Center [NCGC] MLSCN Grant: 1 X01 MH079860-01 Assay Provider: Elisabeth D. Martinez, University of Texas SW Medical Center NCGC Assay Overview: This secondary assay characterizes selected actives identified in the qHTS of the Locus Derepression (LDR) assay (PubChem AID 597). The LDR assay detects the derepression of a GFP reporter that is stably integrated in a region of the genome of murine c127i mammary cells that... aid1037.table aid1037.tbin
1038 9 NIH Molecular Libraries Screening Centers Network [MLSCN] NIH Chemical Genomics Center [NCGC] MLSCN Grant: 1 X01 MH079860-01 Assay Provider: Elisabeth D. Martinez, University of Texas SW Medical Center NCGC Assay Overview: This secondary assay characterizes selected actives identified in the qHTS of the Locus Derepression (LDR) assay (PubChem AID 597). The LDR assay detects the derepression of a GFP reporter that is stably integrated in a region of the genome of murine c127i mammary cells that... aid1038.table aid1038.tbin
1039 9 NIH Molecular Libraries Screening Centers Network [MLSCN] NIH Chemical Genomics Center [NCGC] MLSCN Grant: 1 X01 MH079860-01 Assay Provider: Elisabeth D. Martinez, University of Texas SW Medical Center NCGC Assay Overview: This secondary assay characterizes selected actives identified in the qHTS of the Locus Derepression (LDR) assay (PubChem AID 597). The LDR assay detects the derepression of a GFP reporter that is stably integrated in a region of the genome of murine c127i mammary cells that... aid1039.table aid1039.tbin
1040 196255 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute (TSRI) Assay Provider: Claes Wahlestedt, TSRI Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number 1 R21 NS056950-01 Grant Proposal PI: Claes Wahlestedt External Assay ID: NPY-Y1_ANT_CNGC_1536_%INH Name: Primary cell-based high-throughput screening assay for antagonists of NPY-Y1 Description: Neuropeptide Y (NPY) is a neurotransm... aid1040.table aid1040.tbin
1041 13 NIH Molecular Libraries Screening Centers Network [MLSCN] NIH Chemical Genomics Center [NCGC] MLSCN Grant: 1 X01 MH079860-01 Assay Provider: Elisabeth D. Martinez, University of Texas SW Medical Center NCGC Assay Overview: This secondary assay characterizes selected actives identified in the qHTS of the Locus Derepression (LDR) assay (PubChem AID 597). The LDR assay detects the derepression of a GFP reporter that is stably integrated in a region of the genome of murine c127i mammary cells that... aid1041.table aid1041.tbin
1042 13 NIH Molecular Libraries Screening Centers Network [MLSCN] NIH Chemical Genomics Center [NCGC] MLSCN Grant: 1 X01 MH079860-01 Assay Provider: Elisabeth D. Martinez, University of Texas SW Medical Center NCGC Assay Overview: This secondary assay characterizes selected actives identified in the qHTS of the Locus Derepression (LDR) assay (PubChem AID 597). The LDR assay detects the derepression of a GFP reporter that is stably integrated in a region of the genome of murine c127i mammary cells that... aid1042.table aid1042.tbin
1043 12 NIH Molecular Libraries Screening Centers Network [MLSCN] NIH Chemical Genomics Center [NCGC] MLSCN Grant: 1 X01 MH079860-01 Assay Provider: Elisabeth D. Martinez, University of Texas SW Medical Center NCGC Assay Overview: This secondary assay characterizes selected actives identified in the qHTS of the Locus Derepression (LDR) assay (PubChem AID 597). The LDR assay detects the derepression of a GFP reporter that is stably integrated in a region of the genome of murine c127i mammary cells that... aid1043.table aid1043.tbin
1044 15999 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute, TSRI Assay Provider: The Scripps Research Institute Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 1 R03 MH076533-01 Grant Proposal PI: Germana Sanna External Assay ID: S1P1_AG_BLA_384_%ACT Name: Primary cell-based high-throughput screening assay to identify agonists of Sphingosine 1-Phosphate receptor 1 (S1P1) Descri... aid1044.table aid1044.tbin
1045 1515 Southern Research Molecular Libraries Screening Center (SRMLSC) Southern Research Institute (Birmingham, Alabama) NIH Molecular Libraries Screening Centers Network (MLSCN) Submitted by Dr. Todd Waldman (Lombardi Cancer Center, Georgetown University School of Medicine) Award: R03-NS053741 Numerous genes have been identified which participate, either through activation (oncogenes) or inactivation (tumor suppressors), in the multifactorial process of carcinogenesis (Vogelstein B and Kinzler KW, 200... aid1045.table aid1045.tbin
1046 217250 Molecular Library Screening Center Network (MLSCN) Penn Center for Molecular Discovery (PCMD) Assay Provider: Scott L. Diamond, University of Pennsylvania MLSCN Grant: X01-MH076406-01 Target Prothrombin, a 72 kDa blood zymogen (plasma concentration = 2 uM, (1)) is converted to thrombin by factor Xa (FXa) in the prothrombinase complex on platelets by cleavage of R271 and R320. Thrombin then further processes itself by cleavage at R155 and R284 in order to remove prothrombin fragment 1 and fra... aid1046.table aid1046.tbin
1047 1515 Southern Research Molecular Libraries Screening Center (SRMLSC) Southern Research Institute (Birmingham, Alabama) NIH Molecular Libraries Screening Centers Network (MLSCN) Submitted by Dr. Todd Waldman (Lombardi Cancer Center, Georgetown University School of Medicine) Award: R03-NS053741 Numerous genes have been identified which participate, either through activation (oncogenes) or inactivation (tumor suppressors), in the multifactorial process of carcinogenesis (Vogelstein B and Kinzler KW, 200... aid1047.table aid1047.tbin
1048 99367 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute, TSRI Assay Provider: Scripps Florida Network: Molecular Library Screening Center Network (MLSCN) Proposal Number: 1 X01 MH079861-01 Grant Proposal PI: Pat Griffin, TSRI External Assay ID: PPARgSRC3_ARTEFACT_TRFRET_1536_RAW RATIO Name: Measurement of TR-FRET detection format artefact in the screen for agonists of steroid receptor coactivator 3 (SRC-3) recruit... aid1048.table aid1048.tbin
1049 196255 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute (TSRI) Assay Provider: Pat Griffin, TSRI Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 1 X01 MH079861-01 Grant Proposal PI: Pat Griffin, TSRI External Assay ID: PPARgSRC2_ARTEFACT_TRFRET_1536_RAW RATIO Name: Measurement of TR-FRET detection format artefact in the screen for agonists of steroid receptor coactivator 2 (SRC-2... aid1049.table aid1049.tbin
1050 11 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Affiliation: The Scripps Florida Research Institute, TSRI Assay Provider: Steven Brown, TSRI Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 1 R21 NS057101-01 Grant Proposal PI: Steven Brown External Assay ID: GALR2_AG_BLA_1536_EC50 Name: Dose Response cell-based high-throughput screening assay to identify agonists of galanin receptor 2 (GALR2) Description: Galanin, a 29 am... aid1050.table aid1050.tbin
1051 99367 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute (TSRI) Assay Provider: Pat Griffin, TSRI Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 1 X01 MH079861-01 Grant Proposal PI: Pat Griffin, TSRI External Assay ID: PPARgSRC1_ARTEFACT_TRFRET_1536_RAW RATIO Name: Measurement of TR-FRET detection format artefact in the screen for agonists of steroid receptor coactivator 1 (SRC-1... aid1051.table aid1051.tbin
1052 112 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute(SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Number: XO1 MH076390-01 Assay Provider: Dr. John Lazo, University of Pittsburg This assay is designed as a counter-screen for the MKP-3 in vitro HTS assay (AID 425) aimed at identification of compounds with time-dependent behavior. MKP-3 (mitogen-activated protein kinase p... aid1052.table aid1052.tbin
1053 184 List of compounds to be tested: 184 purchased chemical analogs of selected hits identified in the in vitro PLK1-PBD binding primary screen AID 693, and confirmed in the 10-point concentration response assay AID 877. Extracted from the MH078944 proposal submitted by Dr. Michael Yaffe of MIT. The Polo-like kinases (Plks) play important roles in many cell cycle-related events including the initiation of mitosis, chromosome segregation, centrosome maturation, bipolar spindle formation, regulation o... aid1053.table aid1053.tbin
1054 136 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Number: XO1 MH076390-01 Assay Provider: Dr. John Lazo, University of Pittsburg This assay is designed as a counter-screen for the MKP-3 in vitro HTS assay (AID 425) aimed at identification of compounds modulating the redox state of the enzyme active site. MKP-3 (mitogen-a... aid1054.table aid1054.tbin
1055 193 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Number: XO1 MH076390-01 Assay Provider: Dr. John Lazo, University of Pittsburg This MKP-3 dose response assay is developed and performed for the purpose of SAR study on analogs of hits originally identified in the MKP-3 in vitro HTS assay (AID 425) MKP-3 (mitogen-activated... aid1055.table aid1055.tbin
1056 485 Sanford-Burnham Center for Chemical Genomics (SBCCG) Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Number: MH077602-01 Assay Provider: Dr. Jose Luis Millan, Sanford-Burnham Medical Research Institute, San Diego, CA This TNAP dose response assay is developed and performed for the purpose of SAR study on analogs of hits originally identified in the TNAP luminescent HTS assay (AID 518) Alkaline phosphatase (EC 3.1... aid1056.table aid1056.tbin
1057 33 List of compounds to be tested: 33 component starting materials and chemical analogs of the hits identified in the in vitro PLK1-PBD binding primary screen AID 693, and confirmed in the 10-point concentration response assay AID 877. None of the chemistry analogs tested were confirmed active. Extracted from the MH078944 proposal submitted by Dr. Michael Yaffe of MIT. The Polo-like kinases (Plks) play important roles in many cell cycle-related events including the initiation of mitosis, chromosom... aid1057.table aid1057.tbin
1058 37 NIH Molecular Libraries Screening Centers Network [MLSCN] Emory Chemical Biology Discovery Center in MLSCN Assay provider: F.M. Hoffmann, University of Wisconsin-Madison MLSCN Grant: 1R21NS057002-01 Assay Overview: Transforming growth factor beta (TGF-Beta) regulates a variety of processes in mammalian cells, including proliferation, apoptosis, cell migration and extracellular matrix production. Aberrant increases in TGF-Beta signaling have been implicated in several pathological conditions ... aid1058.table aid1058.tbin
1059 112 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Number: XO1 MH077603-01 Assay Provider: Dr. Tomas Mustelin, Sanford-Burnham Medical Research Institute This HePTP dose response assay is developed and performed for the purpose of SAR study on analogs of hits originally identified in the HePTPcolorimetric HTS assay (AID 521)... aid1059.table aid1059.tbin
1060 354 NIH Molecular Libraries Screening Centers Network [MLSCN] Emory Chemical Biology Discovery Center in MLSCN Assay provider: John A. Katzenellenbogen, University of Illinois Urbana-Champaign MLSCN Grant: 1 X01MH78953-01 Assay Overview Estrogens, which are responsible for the growth of many breast cancers, act through the estrogen receptors, ER-alpha and ER-beta, which are ligand-modulated transcription factors and members of the nuclear receptor gene superfamily. ER-alpha and ER-beta are wel... aid1060.table aid1060.tbin
1061 6 Principal Investigator: Bruce S. Edwards, Ph.D Grant: NIH 1R03MH076381-01 Screening Center: New Mexico Molecular Libraries Screening Center Assay Background and Significance Formyl peptide receptors. The G-protein coupled formylpeptide receptor (FPR) was one of the originating members of the chemoattractant receptor superfamily (Le et al., 2002a; Oppenheim et al., 1991). N-formylated peptides such as fMLF are high affinity FPR ligands that trigger a variety of biologic activities in myeloid ce... aid1061.table aid1061.tbin
1062 21 University of New Mexico Assay Overview: Assay Support: NIH 1 X01 MH077613-01 Assay for disassembly of the 26S Proteasome PI: Dorota Skowyra, PhD Assay implementation: Peter Simons PhD, Irena Ivnitski-Steele PhD, Terry Foutz BS, Mark Carter MS, Anna Waller PhD Target Team Leader for the Center: Larry Sklar (lsklar@salud.unm.edu) Assay Background and Significance The 26S proteasome is a highly conserved ATP-dependent protease that plays a central role in the control of protein stability in all ... aid1062.table aid1062.tbin
1063 196173 No grant number Infection with Leishmania represents a major health concern in the developing world, with approximately 1.2 to 1.5 million cases reported annually and 350 million people (globally) at risk of infection. The limited number of available leishmaniasis treatments is complicated by (1) serious (toxic) side effects; and (2) an increase in chemoresistance. Therefore, the identification of new small molecules for the treatment of leishmaniasis is a critical. A simple, inexpensive and... aid1063.table aid1063.tbin
1064 5329 SMM on stem cell growth factors aid1064.table aid1064.tbin
1065 5329 SMM screen on torsin A, vimentin, XIP, LULL1 and LAP1 aid1065.table aid1065.tbin
1066 194423 Southern Research Molecular Libraries Screening Center (SRMLSC) Southern Research Institute (Birmingham, Alabama) NIH Molecular Libraries Screening Centers Network (MLSCN) Submitted by Dr. Cynthia Stauffacher (Purdue University) Award: 1-R03-MH082373-01 A number of common human pathogens, including Enterococcus faecalis, Streptococcus pneumoniae, and Staphylococcus aureus, are becoming progressively more resistant to antibiotics and pose a serious public health threat, especially to post-surgic... aid1066.table aid1066.tbin
1067 5329 SMM on stem cell growth factors aid1067.table aid1067.tbin
1068 35 NIH Molecular Libraries Screening Centers Network [MLSCN] Emory Chemical Biology Discovery Center in MLSCN Assay provider: Keith D. Wilkinson, Emory University MLSCN Grant: 1 R03 MH076382-01 Assay Overview: BAP1 (BRCA1 associated protein 1) is a member of the Ubiquitin carboxy-terminal hydrolase (UCH) family of deubiquitinating enzymes (DUB). We have identified small molecule inhibitors of BAP1 using a kinetic, fluorescence intensity high-throughput screen. In this assay, ubiquitin conjugated... aid1068.table aid1068.tbin
1069 430 University of New Mexico Assay Overview Assay Support: 1 X01 MH078937-01 MLSCN Assay for Activators of Prostate Cell Differentiation PI: Todd A. Thompson, PhD Assay Implementation: Mark Haynes PhD, Mark Carter MS, Anna Waller PhD Target Team Leader for the Center: Eric Prossnitz (EProssnitz@salud.unm.edu) Assay Background and Significance: Prostate cancer is the third leading cause of cancer-related deaths among men in the United States [Jemal A, et al. 2005]. Although there is no known etiolo... aid1069.table aid1069.tbin
1070 141 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Number: XO1 MH077632-01 Assay Provider: Dr. John C. Reed, Sanford-Burnham Medical Research Institute This Bfl-1 dose response assay is developed and performed for the purpose of SAR study on analogs of hits originally identified in the Bfl-1 fluorescence polarization HTS ass... aid1070.table aid1070.tbin
1071 5329 SMM on stem cell growth factors aid1071.table aid1071.tbin
1072 52 Sanford-Burnham Center for Chemical Genomics (SBCCG) Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) NIH Molecular Libraries Screening Centers Network (MLSCN) MLSCN Grant: XO1 MH079863-01 Assay Provider: Dr. John C. Reed, Sanford-Burnham Medical Research Institute This Hsp70 dose response assay is developed and performed for the purpose of SAR study on analogs of hits originally identified in the fluorescence polarization HTS assay for Hsp70 Inhibitors (AID 583). Over-express... aid1072.table aid1072.tbin
1073 5329 SMM on stem cell growth factors aid1073.table aid1073.tbin
1074 10 Emory Chemistry-Biology Discovery Center Assay Overview: Grant number: none Paramyxoviruses comprise several major human pathogens. Although a live-attenuated vaccine protects against measles virus (MV), a member of the paramyxovirus family, the virus remains a principal cause of worldwide mortality and accounts for approximately 21 million cases and 300,000 to 400,000 deaths annually. The development of novel antivirals that allow improved case management of severe measles and silence viral out... aid1074.table aid1074.tbin
1075 5329 SMM screen on proteins relevant to psychiatric diseases aid1075.table aid1075.tbin
1076 430 University of New Mexico Assay Overview Assay Support: 1 X01 MH078937-01 MLSCN Assay for Activators of Prostate Cell Differentiation PI: Todd A. Thompson, PhD Assay Implementation: Mark Haynes PhD, Mark Carter MS, Anna Waller PhD Target Team Leader for the Center: Eric Prossnitz (EProssnitz@salud.unm.edu) Assay Background and Significance: Prostate cancer is the third leading cause of cancer-related deaths among men in the United States [Jemal A, et al. 2005]. Although there is no known etiol... aid1076.table aid1076.tbin
1077 245 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Number: XO1 MH077603-01 Assay Provider: Dr. Tomas Mustelin, Sanford-Burnham Medical Research Institute Protein tyrosine phosphatases (PTPs), working with protein tyrosine kinases (PTKs), control the phosphorylation state of many proteins in the signal transduction pathways.... aid1077.table aid1077.tbin
1078 24 NIH Molecular Libraries Screening Centers Network [MLSCN] Emory Chemical Biology Discovery Center in MLSCN Assay provider: John A. Katzenellenbogen, University of Illinois Urbana-Champaign MLSCN Grant: 1 X01MH78953-01 Assay Overview Menopause is associated with the onset of hot flashes, night sweats, mood changes, and urogenital atrophy, which many women find distressing enough to seek medical management for relief. Estrogens in the form of hormone therapy (HT) have been the standard treatmen... aid1078.table aid1078.tbin
1079 23 NIH Molecular Libraries Screening Centers Network [MLSCN] Emory Chemical Biology Discovery Center in MLSCN Assay provider: John A. Katzenellenbogen, University of Illinois at Urbana-Champaign MLSCN Grant: 1 X01MH78953-01 Assay Overview Estrogens, which are responsible for the growth of many breast cancers, act through the estrogen receptors, ER-alpha and ER-beta, which are ligand-modulated transcription factors and members of the nuclear receptor gene superfamily. ER-alpha and ER-beta are ... aid1079.table aid1079.tbin
1080 1344 NIH Molecular Libraries Screening Centers Network [MLSCN] Emory Chemical Biology Discovery Center in MLSCN Assay provider: Dr. Raymond Dingledine, Emory University MLSCN Grant: 5-U01NS058158-02 Assay Overview: Injury of the brain is a major cause of death and morbidity after the prolonged seizures termed status epilepticus (SE). Studies in rodents have demonstrated that cyclooxygenase 2 (COX2) activation by ischemia and SE generally contributes to neuronal injury, but multiple downstream COX2 si... aid1080.table aid1080.tbin
1081 5329 SMM screen on proteins relevant to psychiatric diseases aid1081.table aid1081.tbin
1082 38 List of compounds to be tested: 37 component starting materials and chemical analogs of the 861574 hit identified in the in vitro PLK1-PBD binding primary screen AID 693, and confirmed in the 10-point concentration response assay AID 877. Extracted from the MH078944 proposal submitted by Dr. Michael Yaffe of MIT. The Polo-like kinases (Plks) play important roles in many cell cycle-related events including the initiation of mitosis, chromosome segregation, centrosome maturation, bipolar spindle... aid1082.table aid1082.tbin
1083 184 List of compounds to be tested: 184 purchased chemical analogs of selected hits identified in the in vitro PLK1-PBD binding primary screen AID 693, and confirmed in the 10-point concentration response assay AID 877. Extracted from the MH078944 proposal submitted by Dr. Michael Yaffe of MIT. The Polo-like kinases (Plks) play important roles in many cell cycle-related events including the initiation of mitosis, chromosome segregation, centrosome maturation, bipolar spindle formation, regulation o... aid1083.table aid1083.tbin
1084 5329 muscle differentiation and reporter gene assay for inhibition of FRG1 aid1084.table aid1084.tbin
1085 218788 NIH Molecular Libraries Screening Centers Network [MLSCN] Emory Chemical Biology Discovery Center in MLSCN Assay provider: Theodore Jardetzky; Northwestern University MLSCN Grant: 1R21NS059415-01 Epstein-Barr virus (EBV), or human herpes virus 4 (HHV-4), is a member of the larger herpesvirus family that consists of three subfamilies (&#945;, &#946;, &#947;). Epstein-Barr virus (EBV) is an extremely prevalent human herpesvirus. Disease syndromes in humans caused by EBV reflect the cell types that... aid1085.table aid1085.tbin
1086 5329 SMM on stem cell growth factors aid1086.table aid1086.tbin
1087 5329 SMM on stem cell growth factors aid1087.table aid1087.tbin
1088 5329 SMM screen for Sam68 and PRMT1 aid1088.table aid1088.tbin
1089 5329 comparative chemical genomics in yeasts aid1089.table aid1089.tbin
1090 5329 comparative chemical genomics in yeasts aid1090.table aid1090.tbin
1091 5329 comparative chemical genomics in yeasts aid1091.table aid1091.tbin
1092 5329 SMM screen on torsin A, vimentin, XIP, LULL1 and LAP1 aid1092.table aid1092.tbin
1093 5329 E. coli filamentation assay aid1093.table aid1093.tbin
1094 5329 SMM on stem cell growth factors aid1094.table aid1094.tbin
1095 5329 SMM on stem cell growth factors aid1095.table aid1095.tbin
1096 5329 SMM on stem cell growth factors aid1096.table aid1096.tbin
1097 5329 SMM on stem cell growth factors aid1097.table aid1097.tbin
1098 5329 SMM screen for pfSir2 binders aid1098.table aid1098.tbin
1099 5329 SMM on stem cell growth factors aid1099.table aid1099.tbin
1100 5329 SMM on stem cell growth factors aid1100.table aid1100.tbin
1101 5329 mammalian splicing inhibition reporter gene assay aid1101.table aid1101.tbin
1102 5329 SMM on stem cell growth factors aid1102.table aid1102.tbin
1103 5329 SMM screen on proteins relevant to psychiatric diseases aid1103.table aid1103.tbin
1104 5329 SMM on stem cell growth factors aid1104.table aid1104.tbin
1105 5329 SMM on stem cell growth factors aid1105.table aid1105.tbin
1106 5329 SMM on stem cell growth factors aid1106.table aid1106.tbin
1107 5329 SMM on stem cell growth factors aid1107.table aid1107.tbin
1108 5329 SMM screen of HPV-E7 aid1108.table aid1108.tbin
1109 5329 hepatitis C virus replication reporter gene assay aid1109.table aid1109.tbin
1110 5329 SMM on stem cell growth factors aid1110.table aid1110.tbin
1111 5329 SMM vanguard set to annotate compounds that bind to a set of control proteins aid1111.table aid1111.tbin
1112 5329 SMM vanguard set to annotate compounds that bind to a set of control proteins aid1112.table aid1112.tbin
1113 5329 SMM on stem cell growth factors aid1113.table aid1113.tbin
1114 5329 SMM on stem cell growth factors aid1114.table aid1114.tbin
1115 5329 SMM on stem cell growth factors aid1115.table aid1115.tbin
1116 5329 SMM screen on torsin A, vimentin, XIP, LULL1 and LAP1 aid1116.table aid1116.tbin
1117 5329 SMM vanguard set to annotate compounds that bind to a set of control proteins aid1117.table aid1117.tbin
1118 5329 SMM vanguard set to annotate compounds that bind to a set of control proteins aid1118.table aid1118.tbin
1119 5329 SMM screen on torsin A, vimentin, XIP, LULL1 and LAP1 aid1119.table aid1119.tbin
1120 5329 SMM on stem cell growth factors aid1120.table aid1120.tbin
1121 5329 SMM on stem cell growth factors aid1121.table aid1121.tbin
1122 5329 SMM vanguard set to annotate compounds that bind to a set of control proteins aid1122.table aid1122.tbin
1123 5329 SMM screen on torsin A, vimentin, XIP, LULL1 and LAP1 aid1123.table aid1123.tbin
1124 5329 SMM screen on torsin A, vimentin, XIP, LULL1 and LAP1 aid1124.table aid1124.tbin
1125 5329 SMM screen for Sam68 and PRMT1 aid1125.table aid1125.tbin
1126 5329 SMM on stem cell growth factors aid1126.table aid1126.tbin
1127 5329 SMM vanguard set to annotate compounds that bind to a set of control proteins aid1127.table aid1127.tbin
1128 5329 SMM vanguard set to annotate compounds that bind to a set of control proteins aid1128.table aid1128.tbin
1129 5329 SMM screen of HPV-E7 aid1129.table aid1129.tbin
1130 5329 SMM screen for focal adhesion protein binders aid1130.table aid1130.tbin
1131 5329 SMM vanguard set to annotate compounds that bind to a set of control proteins aid1131.table aid1131.tbin
1132 5329 SMM on stem cell growth factors aid1132.table aid1132.tbin
1133 5329 SMM on stem cell growth factors aid1133.table aid1133.tbin
1134 32 NIH Molecular Libraries Screening Centers Network [MLSCN] Emory Chemical Biology Discovery Center in MLSCN Grant number: 1 R03 MH076382-01 BAP1 (BRCA1 associated protein 1) is a member of the Ubiquitin carboxy-terminal hydrolase (UCH) family of deubiquitinating enzymes (DUB). The importance of ubiquitin conjugation in many cellular processes suggests a critical role of DUBs in normal physiology and potentially in pathological conditions. Small molecule BAP1 inhibitors were identified using a k... aid1134.table aid1134.tbin
1135 195624 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Proposal Number: MH077602-01 Assay Provider: Dr. Jose Luis Millan, Sanford-Burnham Medical Research Institute, San Diego, CA Alkaline phosphatases (EC 3.1.3.1) (APs) catalyze the hydrolysis of phosphomonoesters, releasing phosphate and alcohol. APs are dimeric enzymes found ... aid1135.table aid1135.tbin
1136 195624 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Proposal Number: R03 MH082385-01 Assay Provider: Dr. Jose Luis Millan, Sanford-Burnham Medical Research Institute, San Diego, CA Alkaline phosphatases (EC 3.1.3.1) (APs) catalyze the hydrolysis of phosphomonoesters, releasing phosphate and alcohol. APs are dimeric enzymes f... aid1136.table aid1136.tbin
1137 8 NIH Molecular Libraries Screening Centers Network [MLSCN] Emory Chemical Biology Discovery Center in MLSCN Assay provider: Dr. Eric Sandberg, ZYGOGEN, LLC MLSCN Grant: 1 X01 MH077629-01 Assay Overview: Pathological angiogenesis contributes to over 70 diseases, including cancer, age-related macular degeneration and rheumatoid arthritis. Current in vitro models employed in screening compounds for effects on angiogenesis lack the biological complexity of in vivo systems. Zygogen, LLC developed a r... aid1137.table aid1137.tbin
1138 5329 chemical-genetic profiling of PK04 Diversity Set aid1138.table aid1138.tbin
1139 5329 SMM on stem cell growth factors aid1139.table aid1139.tbin
1140 5329 SMM vanguard set to annotate compounds that bind to a set of control proteins aid1140.table aid1140.tbin
1141 5329 luciferase inhibition assay aid1141.table aid1141.tbin
1142 5329 SMM on stem cell growth factors aid1142.table aid1142.tbin
1143 5329 hepatitis C virus replication reporter gene assay aid1143.table aid1143.tbin
1144 5003 Pseudomonas biofilm formation assay aid1144.table aid1144.tbin
1145 5329 mammalian CREB reporter gene assay aid1145.table aid1145.tbin
1146 5329 mammalian CREB reporter gene assay aid1146.table aid1146.tbin
1147 5329 SMM vanguard set to annotate compounds that bind to a set of control proteins aid1147.table aid1147.tbin
1148 5329 SMM on stem cell growth factors aid1148.table aid1148.tbin
1149 5329 SMM screen on torsin A, vimentin, XIP, LULL1 and LAP1 aid1149.table aid1149.tbin
1150 5329 SMM screen on torsin A, vimentin, XIP, LULL1 and LAP1 aid1150.table aid1150.tbin
1151 5329 SMM on stem cell growth factors aid1151.table aid1151.tbin
1152 5329 E. coli filamentation assay aid1152.table aid1152.tbin
1153 5329 SMM on stem cell growth factors aid1153.table aid1153.tbin
1154 5329 permeant solute osmotic lysis assay aid1154.table aid1154.tbin
1155 5329 permeant solute osmotic lysis assay aid1155.table aid1155.tbin
1156 5329 permeant solute osmotic lysis assay aid1156.table aid1156.tbin
1157 5329 permeant solute osmotic lysis assay aid1157.table aid1157.tbin
1158 5329 cytoblot for phosphorylation of S6 protein kinase aid1158.table aid1158.tbin
1159 5329 mammalian NOX2/4/5 superoxide generation assay aid1159.table aid1159.tbin
1160 5329 mammalian NOX2/4/5 superoxide generation assay aid1160.table aid1160.tbin
1161 5329 mammalian NOX2/4/5 superoxide generation assay aid1161.table aid1161.tbin
1162 5329 chemical-genetic profiling of PK04 Diversity Set aid1162.table aid1162.tbin
1163 5329 chemical-genetic profiling of PK04 Diversity Set aid1163.table aid1163.tbin
1164 5329 chemical-genetic profiling of PK04 Diversity Set aid1164.table aid1164.tbin
1165 5329 chemical-genetic profiling of PK04 Diversity Set aid1165.table aid1165.tbin
1166 5329 chemical-genetic profiling of PK04 Diversity Set aid1166.table aid1166.tbin
1167 5329 chemical-genetic profiling of PK04 Diversity Set aid1167.table aid1167.tbin
1168 5329 chemical-genetic profiling of PK04 Diversity Set aid1168.table aid1168.tbin
1169 5329 chemical-genetic profiling of PK04 Diversity Set aid1169.table aid1169.tbin
1170 5329 chemical-genetic profiling of PK04 Diversity Set aid1170.table aid1170.tbin
1171 5329 chemical-genetic profiling of PK04 Diversity Set aid1171.table aid1171.tbin
1172 5329 chemical-genetic profiling of PK04 Diversity Set aid1172.table aid1172.tbin
1173 5329 comparative chemical genomics in yeasts aid1173.table aid1173.tbin
1174 5329 chemical-genetic profiling of PK04 Diversity Set aid1174.table aid1174.tbin
1175 5329 enzyme inhibition of dihydroorotate dehydrogenase aid1175.table aid1175.tbin
1176 5003 Pseudomonas biofilm formation assay aid1176.table aid1176.tbin
1177 5329 comparative chemical genomics in yeasts aid1177.table aid1177.tbin
1178 5329 E. coli filamentation assay aid1178.table aid1178.tbin
1179 5329 E. coli filamentation assay aid1179.table aid1179.tbin
1180 5329 chemical-genetic profiling of PK04 Diversity Set aid1180.table aid1180.tbin
1181 5329 SMM screen for proteins involved in apoptosis aid1181.table aid1181.tbin
1182 5329 SMM screen on torsin A, vimentin, XIP, LULL1 and LAP1 aid1182.table aid1182.tbin
1183 5329 SMM screen on torsin A, vimentin, XIP, LULL1 and LAP1 aid1183.table aid1183.tbin
1184 5329 Plasmodium whole-cell live/dead viability assay aid1184.table aid1184.tbin
1185 5329 fluorescence polarization screen for Hox protein:DNA interaction aid1185.table aid1185.tbin
1186 4423 imaging screen for inhibition of hydroxyurea-induced DNA damage aid1186.table aid1186.tbin
1187 4423 imaging screen for inhibition of hydroxyurea-induced DNA damage aid1187.table aid1187.tbin
1188 617 The EPA Fathead Minnow Acute Toxicity database was generated by the U.S. EPA Mid-Continental Ecology Division (MED) for the purpose of developing an expert system to predict acute toxicity from chemical structure based on mode of action considerations. Hence, an important and unusual characteristic of this toxicity database is that the 617 tested industrial organic chemicals were expressly chosen to serve as a useful training set for development of predictive quantitative structure-activity relat... aid1188.table aid1188.tbin
1189 1547 The Carcinogenic Potency Database (CPDB) is a unique and widely used international resource of the results of over 6500 chronic, long-term animal cancer tests on over 1500 chemical substances. The CPDB provides easy access to the bioassay literature, with qualitative and quantitative analyses of both positive and negative experiments that have been published over the past 50 years in the general literature and by the National Cancer Institute/National Toxicology Program. The CPDB standardizes the... aid1189.table aid1189.tbin
1190 32 The Carcinogenic Potency Database (CPDB) is a unique and widely used international resource of the results of over 6500 chronic, long-term animal cancer tests on over 1500 chemical substances. The CPDB provides easy access to the bioassay literature, with qualitative and quantitative analyses of both positive and negative experiments that have been published over the past 50 years in the general literature and by the National Cancer Institute/National Toxicology Program. The CPDB standardizes the... aid1190.table aid1190.tbin
1191 87 The Carcinogenic Potency Database (CPDB) is a unique and widely used international resource of the results of over 6500 chronic, long-term animal cancer tests on over 1500 chemical substances. The CPDB provides easy access to the bioassay literature, with qualitative and quantitative analyses of both positive and negative experiments that have been published over the past 50 years in the general literature and by the National Cancer Institute/National Toxicology Program. The CPDB standardizes the... aid1191.table aid1191.tbin
1192 10 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute (TSRI) Assay Provider: Hugh Rosen, TSRI Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 1 R03 MH076533-01 Grant Proposal PI: Germana Sanna External Assay ID: S1P3_AG_BLA_384_EC50_Purchased_Analogues Name: Dose Response Cell-Based Assay for Agonists of the Sphingosine 1-Phosphate Receptor 3 (S1P3): Purchased Analogues De... aid1192.table aid1192.tbin
1193 53 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) MLSCN Grant: XO1 MH079863-01 Assay Provider: Dr. John C. Reed, Sanford-Burnham Medical Research Institute ) This Hsc70 dose response assay is developed and performed to study the specificity of analogs of hits tested in the "In Vitro Hsp70 Dose Response Fluorescence Polarization... aid1193.table aid1193.tbin
1194 860 The Carcinogenic Potency Database (CPDB) is a unique and widely used international resource of the results of over 6500 chronic, long-term animal cancer tests on over 1500 chemical substances. The CPDB provides easy access to the bioassay literature, with qualitative and quantitative analyses of both positive and negative experiments that have been published over the past 50 years in the general literature and by the National Cancer Institute/National Toxicology Program. The CPDB standardizes the... aid1194.table aid1194.tbin
1195 1216 The Food and Drug Administration (FDA) Center for Drug Evaluation and Research, Office of Pharmaceutical Science, Informatics and Computational Safety Analysis Staff's Maximum Recommended Daily Dose (FDAMDD) database contains values for over 1200 pharmaceuticals listed in Martindale: The Extra Pharmacopoeia (1973, 1983, and 1993) and The Physicians' Desk Reference (1995 and 1999). Some classes of chemicals were excluded from the FDAMDD database due to their unsuitability for most QSAR modeling pr... aid1195.table aid1195.tbin
1196 95 University of New Mexico Assay Overview Assay Support: 1 X01 MH078937-01 MLSCN Assay for Activators of Prostate Cell Differentiation PI: Todd A. Thompson, PhD Assay Implementation: Mark Haynes PhD, Mark Carter MS, Anna Waller PhD, Target Team Leader for the Center: Eric Prossnitz (EProssnitz@salud.unm.edu) Assay Background and Significance: Prostate cancer is the third leading cause of cancer-related deaths among men in the United States [Jemal A, et al. 2005]. Although there is no known etio... aid1196.table aid1196.tbin
1197 95 University of New Mexico Assay Overview Assay Support: 1 X01 MH078937-01 MLSCN Assay for Activators of Prostate Cell Differentiation PI: Todd A. Thompson, PhD Assay Implementation: Mark Haynes PhD, Mark Carter MS, Anna Waller PhD Target Team Leader for the Center: Eric Prossnitz (EProssnitz@salud.unm.edu) Assay Background and Significance: Prostate cancer is the third leading cause of cancer-related deaths among men in the United States [Jemal A, et al. 2005]. Although there is no known etiol... aid1197.table aid1197.tbin
1198 95 University of New Mexico Assay Overview Assay Support: 1 X01 MH078937-01 MLSCN Assay for Activators of Prostate Cell Differentiation PI: Todd A. Thompson, PhD Assay Implementation: Mark Haynes PhD, Mark Carter MS, Anna Waller PhD, Target Team Leader for the Center: Eric Prossnitz (EProssnitz@salud.unm.edu) Assay Background and Significance: Prostate cancer is the third leading cause of cancer-related deaths among men in the United States [Jemal A, et al. 2005]. Although there is no known etio... aid1198.table aid1198.tbin
1199 1007 The Carcinogenic Potency Database (CPDB) is a unique and widely used international resource of the results of over 6500 chronic, long-term animal cancer tests on over 1500 chemical substances. The CPDB provides easy access to the bioassay literature, with qualitative and quantitative analyses of both positive and negative experiments that have been published over the past 50 years in the general literature and by the National Cancer Institute/National Toxicology Program. The CPDB standardizes the... aid1199.table aid1199.tbin
1200 95 University of New Mexico Assay Overview Assay Support: 1 X01 MH078937-01 MLSCN Assay for Activators of Prostate Cell Differentiation PI: Todd A. Thompson, PhD Assay Implementation: Mark Haynes PhD, Mark Carter MS, Anna Waller PhD Target Team Leader for the Center: Eric Prossnitz (EProssnitz@salud.unm.edu) Assay Background and Significance: Prostate cancer is the third leading cause of cancer-related deaths among men in the United States [Jemal A, et al. 2005]. Although there is no known etiol... aid1200.table aid1200.tbin
1201 209 The DBPCAN data file was derived from data published in [Woo, Y.T., D. Lai, J.L. McLain, M.K. Manibusan, and V. Dellarco (2002) Use of mechanism-based structure-activity relationships analysis in carcinogenic potential ranking for drinking water disinfection by-products, Environ. Health Perspect.,110 Suppl 1: 75-87.]. DBPCAN contains predicted estimates of carcinogenic potential for 209 chemicals detected in finished drinking water samples having undergone water disinfection treatment. Since lit... aid1201.table aid1201.tbin
1202 6 Principal Investigator: Bruce S. Edwards, Ph.D (BEdwards@salud.unm.edu) Grant: NIH 1R03MH076381-01 Screening Center: New Mexico Molecular Libraries Screening Center Background/Significance Formyl peptide receptors. The G-protein coupled formylpeptide receptor(FPR) was one of the originating members of the chemoattractant receptor superfamily (1, 2). N-formylated peptides such as fMLF are high affinity FPR ligands that trigger a variety of biologic activities in myeloid cells, including chemokin... aid1202.table aid1202.tbin
1203 196255 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute (TSRI) Assay Provider: Richard Morimoto, Northwestern University Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 5 R21 NS056337-02 Grant Proposal PI: Richard Morimoto External Assay ID: HSP70_AG_Lumi_1536_% Act Name: Primary cell-based high-throughput screening assay to identify transcriptional activators of heat shock pro... aid1203.table aid1203.tbin
1204 232 Researchers within FDA's National Center for Toxicological Research (NCTR) generated a database of experimental estrogen receptor binding results for the express purpose of developing improved QSAR models to predict ER binding affinities.The NCTR ER database is a structurally diverse set of natural, synthetic, and environmental estrogens covering most known estrogenic classes and spanning a wide range of biological activity. It represents the largest published ER binding database of same-assay re... aid1204.table aid1204.tbin
1205 1152 The Carcinogenic Potency Database (CPDB) is a unique and widely used international resource of the results of over 6500 chronic, long-term animal cancer tests on over 1500 chemical substances. The CPDB provides easy access to the bioassay literature, with qualitative and quantitative analyses of both positive and negative experiments that have been published over the past 50 years in the general literature and by the National Cancer Institute/National Toxicology Program. The CPDB standardizes the... aid1205.table aid1205.tbin
1206 45 Principal Investigator: Hattie D. Gresham, Ph.D. Grant: NIH 1X01MH078952-01 Screening Center: New Mexico Molecular Libraries Screening Center Target Team Leader for the Center: Bruce Edwards (BEdwards@salud.unm.edu) This report summarizes the series of assays used to identify novel small molecule antagonists directed against signaling pathways involved in quorum sensing, a cell-to-cell communication system that permits members of a bacterial population to coordinate their behavior dependent on... aid1206.table aid1206.tbin
1207 3 University of New Mexico Assay Overview: Assay Support: NIH 1 X01 MH077613-01 Assay for disassembly of the 26S Proteasome PI: Dorota Skowyra, PhD Assay implementation: Chris Allen PhD, Peter Simons PhD, Anna Waller PhD, MaryAnn Osley PhD Target Team Leader for the Center: Larry A. Sklar (LSklar@salud.unm.edu) Assay Background and Significance: The 26S proteasome in a highly conserved ATP-dependent protease that plays a central role in the control of protein stability in all eukaryotic cells. I... aid1207.table aid1207.tbin
1208 1240 The Carcinogenic Potency Database (CPDB) is a unique and widely used international resource of the results of over 6500 chronic, long-term animal cancer tests on over 1500 chemical substances. The CPDB provides easy access to the bioassay literature, with qualitative and quantitative analyses of both positive and negative experiments that have been published over the past 50 years in the general literature and by the National Cancer Institute/National Toxicology Program. The CPDB standardizes the... aid1208.table aid1208.tbin
1209 194226 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Number: R03 MH082386-01 Assay Provider: Dr. Hudson H. Freeze, Sanford-Burnham Medical Research Institute, San Diego, CA Congenital Disorders of Glycosylation (CDG) are autosomal recessive defects in the synthesis of N-linked oligosaccharide chains. CDG group I (CDG-I) defec... aid1209.table aid1209.tbin
1210 822 University of New Mexico Assay Overview: Assay Support: 1X01 MH077627-01 Assay for Ligands of GPR30 and Classical Estrogen Receptors PI: Eric Prossnitz, PhD Assay Development: Megan Dennis Assay Implementation: Megan Dennis, Mark Haynes, PhD Target Team Leader for the Center: Eric Prossnitz, PhD (EProssnitz@salud.unm.edu) Assay Background and Significance: The physiological effects of estrogen are diverse and numerous, with roles in growth, development and homeostasis of numerous tissues. Rol... aid1210.table aid1210.tbin
1211 822 University of New Mexico Assay Overview: Assay Support: 1X01 MH077627-01 Assay for Ligands of GPR30 and Classical Estrogen Receptors PI: Eric Prossnitz, PhD Assay Development: Megan Dennis Assay Implementation: Megan Dennis, Mark Haynes, PhD Target Team Leader for the Center: Eric Prossnitz, PhD (EProssnitz@salud.unm.edu) Assay Background and Significance: The physiological effects of estrogen are diverse and numerous, with roles in growth, development and homeostasis of numerous tissues. Rol... aid1211.table aid1211.tbin
1212 151 University of New Mexico Assay Overview: Assay Support: 1X01 MH077627-01 Assay for Ligands of GPR30 and Classical Estrogen Receptors PI: Eric Prossnitz, PhD Assay Development: Megan Dennis Assay Implementation: Megan Dennis, Mark Haynes, PhD Target Team Leader for the Center: Eric Prossnitz, PhD (EProssnitz@salud.unm.edu) Assay Background and Significance: The physiological effects of estrogen are diverse and numerous, with roles in growth, development and homeostasis of numerous tissues. Rol... aid1212.table aid1212.tbin
1213 99 University of New Mexico Assay Overview: Assay Support: 1X01 MH077627-01 Assay for Ligands of GPR30 and Classical Estrogen Receptors PI: Eric Prossnitz, PhD Assay Development: Megan Dennis Assay Implementation: Megan Dennis, Mark Haynes, PhD Target Team Leader for the Center: Eric Prossnitz, PhD (EProssnitz@salud.unm.edu) Assay Background and Significance: The physiological effects of estrogen are diverse and numerous, with roles in growth, development and homeostasis of numerous tissues. ... aid1213.table aid1213.tbin
1214 194226 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Number: R03 MH082386-01 Assay Provider: Dr. Hudson H. Freeze, Sanford-Burnham Medical Research Institute, San Diego, CA Congenital Disorders of Glycosylation (CDG) are autosomal recessive defects in the synthesis of N-linked oligosaccharide chains. CDG group I (CDG-I) defec... aid1214.table aid1214.tbin
1215 529 Molecular Library Screening Center Network (MLSCN) Penn Center for Molecular Discovery (PCMD) Assay Provider: Scott L. Diamond, University of Pennsylvania MLSCN Grant: X01-MH076406-01 Target Prothrombin, a 72 kDa blood zymogen (plasma concentration = 2 uM, (1)) is converted to thrombin by factor Xa (FXa) in the prothrombinase complex on platelets by cleavage of R271 and R320. Thrombin then further processes itself by cleavage at R155 and R284 in order to remove prothrombin fragment 1 and fra... aid1215.table aid1215.tbin
1216 194226 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Number: R03 MH082386-01 Assay Provider: Dr. Hudson H. Freeze, Sanford-Burnham Medical Research Institute, San Diego, CA Congenital Disorders of Glycosylation (CDG) are autosomal recessive defects in the synthesis of N-linked oligosaccharide chains. CDG group I (CDG-I) defec... aid1216.table aid1216.tbin
1217 194226 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Number: R03 MH082386-01 Assay Provider: Dr. Hudson H. Freeze, Sanford-Burnham Medical Research Institute, San Diego, CA Diaphorase is an enzyme which reversibly catalyzes the reaction of converting NAD(P)+ to NAD(P)H and transfers its electrons to a variety of Redox dyes, e.... aid1217.table aid1217.tbin
1218 12 The physiological effects of estrogen are diverse and numerous, with roles in growth, development and homeostasis of numerous tissues. Roles for estrogen in mammalian female reproductive development are among the best defined, but estrogen also plays a part in regulation of skeletal cancer, (cardio)vascular function, the central nervous system as well as in the immune system. Stimulation with estrogen induces many signaling pathways, leading to an array of cellular responses including adhesion, m... aid1218.table aid1218.tbin
1219 12 University of New Mexico Assay Overview: Assay Support: 1X01 MH077627-01 Assay for Ligands of GPR30 and Classical Estrogen Receptors PI: Eric Prossnitz, PhD Assay Development: Megan Dennis Assay Implementation: Megan Dennis, Mark Haynes, PhD Target Team Leader for the Center: Eric Prossnitz, PhD (EProssnitz@salud.unm.edu) Assay Background and Significance: The physiological effects of estrogen are diverse and numerous, with roles in growth, development and homeostasis of many tissues. Rol... aid1219.table aid1219.tbin
1220 194226 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Number: R03 MH082386-01 Assay Provider: Dr. Hudson H. Freeze, Sanford-Burnham Medical Research Institute, San Diego, CA Congenital Disorders of Glycosylation (CDG) are autosomal recessive defects in the synthesis of N-linked oligosaccharide chains. CDG group I (CDG-I) defect... aid1220.table aid1220.tbin
1221 63 University of New Mexico Assay Overview: Assay Support: 1X01 MH077627-01 Assay for Ligands of GPR30 and Classical Estrogen Receptors PI: Eric Prossnitz, PhD Assay Development: Megan Dennis Assay Implementation: Megan Dennis, Mark Haynes, PhD Target Team Leader for the Center: Eric Prossnitz, PhD (EProssnitz@salud.unm.edu) Assay Background and Significance: The physiological effects of estrogen are diverse and numerous, with roles in growth, development and homeostasis of numerous tissues. Rol... aid1221.table aid1221.tbin
1222 187284 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Number: R03 DA024887-01 Assay Provider: Dr. John C. Reed, Sanford-Burnham Medical Research Institute The endoplasmic reticulum (ER) fulfills multiple cellular functions (reviewed in [1-4]). The lumen of the ER contains high concentration of Ca2+ due to the transport of calc... aid1222.table aid1222.tbin
1223 63 University of New Mexico Assay Overview: Assay Support: 1X01 MH077627-01 Assay for Ligands of GPR30 and Classical Estrogen Receptors PI: Eric Prossnitz, PhD Assay Development: Megan Dennis Assay Implementation: Megan Dennis, Mark Haynes, PhD Target Team Leader for the Center: Eric Prossnitz, PhD (EProssnitz@salud.unm.edu) Assay Background and Significance: The physiological effects of estrogen are diverse and numerous, with roles in growth, development and homeostasis of numerous tissues. Rol... aid1223.table aid1223.tbin
1224 10 University of New Mexico Assay Overview: Assay Support: 1X01 MH077627-01 Assay for Ligands of GPR30 and Classical Estrogen Receptors PI: Eric Prossnitz, PhD Assay Development: Megan Dennis Assay Implementation: Megan Dennis, Mark Haynes, PhD Target Team Leader for the Center: Eric Prossnitz, PhD (EProssnitz@salud.unm.edu) Assay Background and Significance: The physiological effects of estrogen are diverse and numerous, with roles in growth, development and homeostasis of numerous tissues. Rol... aid1224.table aid1224.tbin
1225 47 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Affiliation: The Scripps Florida Research Institute, TSRI Assay Provider: Steven Brown, TSRI Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 1 R21 NS057101-01 Grant Proposal PI: Steven Brown External Assay ID: GALR2_ANT_BLA_1536_IC50 Name: Dose Response cell-based high-throughput screening assay to identify antagonists of galanin receptor 2 (GALR2) Description: Galanin, a 2... aid1225.table aid1225.tbin
1226 12 University of New Mexico Assay Overview: Assay Support: 1X01 MH077627-01 Assay for Ligands of GPR30 and Classical Estrogen Receptors PI: Eric Prossnitz, PhD Assay Development: Megan Dennis Assay Implementation: Megan Dennis, Mark Haynes, PhD Target Team Leader for the Center: Eric Prossnitz, PhD (EProssnitz@salud.unm.edu) Assay Background and Significance: The physiological effects of estrogen are diverse and numerous, with roles in growth, development and homeostasis of numerous tissues. Rol... aid1226.table aid1226.tbin
1227 744 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Number: None This glyceraldehydes-3-phosphate dehydrogenase (GAPDH; EC 1.2.1.12) dose-response assay is developed and performed at the Sanford-Burnham Center for Chemical Genomics for characterization of the hits of biochemical assays. GAPDH is found in all mammalian tiss... aid1227.table aid1227.tbin
1228 11 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Affiliation: The Scripps Florida Research Institute (TSRI) Assay Provider: Steven Brown, TSRI Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 1 R21 NS057101-01 Grant Proposal PI: Steven Brown External Assay ID: NFAT_ACT_BLA_1536_EC50 Name: Dose response counterscreen for agonists of galanin receptor 2 (GalR2): a cell-based high-throughput screening assay for activators of bet... aid1228.table aid1228.tbin
1229 194226 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Number: R03 MH082386-01 Assay Provider: Dr. Hudson H. Freeze, Sanford-Burnham Medical Research Institute, San Diego, CA Diaphorase is an enzyme which reversibly catalyzes the reaction of converting NAD(P)+ to NAD(P)H and transfers its electrons to a variety of Redox dyes, su... aid1229.table aid1229.tbin
1230 207898 Molecular Library Screening Center Network (MLSCN) Penn Center for Molecular Discovery (PCMD) Assay Provider: Brent Stockwell, Columbia University MLSCN Grant: R03MH082369-01 The E3 ligases are involved in regulating other proteins by covalent ligation to the 76 amino acid protein ubiquitin. This post-translational modification can result in altered conformation, altered activity, or degradation of the sustrate protein. Thus, E3 ligases are effectors of a major means of post-translational modifi... aid1230.table aid1230.tbin
1231 70 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Number: XO1 MH077632-01 Assay Provider: Dr. John C. Reed, Sanford-Burnham Medical Research Institute Apoptosis is governed in part by B-cell lymphoma-2 (Bcl-2)-family proteins.The human genome contains six genes that encode anti-apoptotic members of the Bcl-2 family of whic... aid1231.table aid1231.tbin
1232 10 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute (TSRI) Assay Provider: Hugh Rosen, TSRI Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 1 R03 MH076533-01 Grant Proposal PI: Germana Sanna External Assay ID: S1P2_AG_BLA_384_EC50_Purchased_S1P3_Analogues Name: Dose Response Cell-Based Assay for Agonists of the Sphingosine 1-Phosphate Receptor 2 (S1P2): Purchased Analogue... aid1232.table aid1232.tbin
1233 635 Southern Research Molecular Libraries Screening Center (SRMLSC) Southern Research Institute (Birmingham, Alabama) NIH Molecular Libraries Screening Centers Network (MLSCN) Submitted by Dr. Cynthia Stauffacher (Purdue University) Award: 1-R03-MH082373-01 A number of common human pathogens, including Enterococcus faecalis, Streptococcus pneumoniae, and Staphylococcus aureus, are becoming progressively more resistant to antibiotics and pose a serious public health threat, especially to post-surgic... aid1233.table aid1233.tbin
1234 61 List of compounds to be tested: active compounds identified in the Redox Cycling H2O2 Generation primary screen AID 878, will be confirmed in 10-point concentration response assays. Hydrogen peroxide (H2O2) can modulate (activate or inhibit) the activity of a variety of proteins including protein kinases, protein phosphatases, transcription factors, phospholipases, ion channels and G proteins. H2O2 is capable of oxidizing the cysteine residues of proteins that may be crucial for their catalytic... aid1234.table aid1234.tbin
1235 62139 Molecular Library Screening Center Network (MLSCN) Penn Center for Molecular Discovery (PCMD) Assay Provider: Scott Diamond, University of Pennsylvania MLSCN Grant: MH076406-01 The alternative complement pathway does not require antibody for its activation. A variety of antigens such as bacterial lipopolysaccharide and components of viruses and other pathogens have the ability to activate this pathway. The complement component C3 is spontaneously cleaved into C3a and C3b fragments. If C3b binds ... aid1235.table aid1235.tbin
1236 218788 NIH Molecular Libraries Screening Centers Network [MLSCN] Emory Chemical Biology Discovery Center in MLSCN Assay provider: Jonathan Glass, MD, Emory University School of Medicine MLSCN Grant: R03DA024890-01 Calpains are ubiquitous, calcium-activated cysteine proteases involved in both physiological and pathological cellular functions. The two major forms, u-calpain (calpain I) and m-calpain (calpain II), are activated by micromolar and millimolar calcium concentrations, respectively. A current... aid1236.table aid1236.tbin
1237 349 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute, TSRI Assay Provider: Peter Tobias, TSRI Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 1 X01 MH079857-01 Grant Proposal PI: Peter Tobias External Assay ID: BLA_INH_BLA_1536_3X%INH Name: Counterscreen for inhibitors of TLR4-MyD88 binding: a cell-based high-throughput screening assay for inhibitors of beta-lactamase activity ... aid1237.table aid1237.tbin
1238 47 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Affiliation: The Scripps Research Institute (TSRI) Assay Provider: Steven Brown, TSRI Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 1 R21 NS057101-01 Grant Proposal PI: Steven Brown External Assay ID: NFAT_INH_BLA_1536_ IC50 Name: Dose response counterscreen for antagonists of galanin receptor 2 (GalR2): a cell-based high-throughput screening assay for inhibitors of beta-la... aid1238.table aid1238.tbin
1239 193297 Southern Research Molecular Libraries Screening Center (SRMLSC) Southern Research Institute (Birmingham, Alabama) NIH Molecular Libraries Screening Centers Network (MLSCN) Submitted by Dr. Maurizio Grimaldi (Neuropharmacology Laboratory, Southern Research Institute) Award: R03 MH082367-01 The pharmacological treatment of neurodegenerative disorders has been a disappointment when compared to the successes obtained in stroke, other neurological diseases like seizures, and in mental health diseases... aid1239.table aid1239.tbin
1240 49567 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA). Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Number: XO1 MH077632-01 Assay Provider: Dr. John C. Reed, Sanford-Burnham Medical Research Institute Bcl-B is an anti-apoptotic member of the Bcl-2 family that is prominently expressed in plasma and multiple myeloma cells. TR3 (NR4A1; HMR; NP10; GFRP1; NAK1; NUR77; NGFIB) ... aid1240.table aid1240.tbin
1241 1242 Southern Research Molecular Libraries Screening Center (SRMLSC) Southern Research Institute (Birmingham, Alabama) NIH Molecular Libraries Screening Centers Network (MLSCN) Award: R03 MH082367-01, Submitted by Dr. Maurizio Grimaldi (Neuropharmacology Laboratory, Southern Research Institute) The pharmacological treatment of neurodegenerative disorders has been a disappointment when compared to the successes obtained in stroke, other neurological diseases like seizures, and in mental health disease... aid1241.table aid1241.tbin
1242 121267 Molecular Library Screening Centre Network (MLSCN) Penn Center for Molecular Discovery (PCMD) Assay Provider: Kim Lewis, Northeastern University, Boston, MA MLSCN Grant: X01 MH080686-01 This screen is for compounds that are potentiators of the antifungal drug clotrimazole that are active against multidrug tolerant persister cells of Candida albicans biofilms. Biofilms are notoriously resistant to antimicrobial therapy, but the mechanism of resistance remains largely unknown. The recently charact... aid1242.table aid1242.tbin
1243 54 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA). Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Number: XO1 MH077632-01 Assay Provider: Dr. John C. Reed, Sanford-Burnham Medical Research Institute This dose response assay is developed and performed for the purpose of confirming hits originally identified in Fluorescence Polarization Screen Assay for Bcl-B Phe... aid1243.table aid1243.tbin
1244 2 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA). Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Number: XO1 MH077632-01 Assay Provider: Dr. John C. Reed, Sanford-Burnham Medical Research Institute This assay is a counter screen for compounds identified in the Bcl-B/FITC-TR3 fluorescence polarization assay (AID 1240). Bcl-B is an anti-apoptotic member ... aid1244.table aid1244.tbin
1245 2 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA). Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Number: XO1 MH077632-01 Assay Provider: Dr. John C. Reed, Sanford-Burnham Medical Research Institute This assay is a counter screen for compounds identified in the Bcl-B/FITC-TR3 fluorescence polarization assay (AID 1240). Bcl-B is an anti-apoptotic member ... aid1245.table aid1245.tbin
1246 118107 Data Source: Columbia University Molecular Screening Center Source (MLSCN Center Name): Columbia University Molecular Screening Center Center Affiliation: Columbia University Molecular Screening Center Assay Provider: Thomas Mayer, Columbia University Molecular Screening Center Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 1R03MH076509-01 TNFalpha induced E-Selectin HCS was developed and screened at the Columbia University Molecular Screening Center as part o... aid1246.table aid1246.tbin
1247 37 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Number: 1R03 DA024887-01 Assay Provider: Dr. John C. Reed, Sanford-Burnham Medical Research Institute This dose response assay is developed and performed in the laboratory of the assay provider for the purpose of SAR study on analogs of benzodiazepine hits originally identif... aid1247.table aid1247.tbin
1248 10 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute (TSRI) Assay Provider: Hugh Rosen, TSRI Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 1 R03 MH076533-01 Grant Proposal PI: Germana Sanna External Assay ID: S1P1_AG_BLA_384_EC50_Purchased_Analogues Name: Dose Response Cell-Based Assay for Agonists of the Sphingosine 1-Phosphate Receptor 1 (S1P1): Purchased Analogues De... aid1248.table aid1248.tbin
1249 695 Data Source: Columbia University Molecular Screening Center Source (MLSCN Center Name): Columbia University Molecular Screening Center Center Affiliation: Columbia University Molecular Screening Center Assay Provider: Thomas Mayer, Columbia University Molecular Screening Center Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 1R03 MH076343-01 During inflammation, cytokine activation of the NFkB signaling pathway results in, among others, VCAM-1 (Vascular Cell Ad... aid1249.table aid1249.tbin
1250 658 Southern Research Molecular Libraries Screening Center (SRMLSC) Southern Research Institute (Birmingham, Alabama) NIH Molecular Libraries Screening Centers Network (MLSCN) Assay Provider: Dr. Qianjun Li, Southern Research Institute Award: R03 MH081 271-01 Arthropod borne viruses (arboviruses) are important human and/or animal pathogens that cause acute virus infections with severe diseases and/or death. Several recent human and/or animal epidemics have been caused by arboviruses, including Dengu... aid1250.table aid1250.tbin
1251 195489 Southern Research Molecular Libraries Screening Center (SRMLSC) Southern Research Institute (Birmingham, Alabama) NIH Molecular Libraries Screening Centers Network (MLSCN) Assay Provider: Dr. Qianjun Li, Southern Research Institute Award: R03 MH081 271-01 Arthropod borne viruses (arboviruses) are important human and/or animal pathogens that cause acute virus infections with severe diseases and/or death. Several recent human and/or animal epidemics have been caused by arboviruses, including Dengu... aid1251.table aid1251.tbin
1252 72 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute (TSRI) Assay Provider: Richard Morimoto, Northwestern University Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 5 R21 NS056337-02 Grant Proposal PI: Richard Morimoto External Assay ID:HSP70_AG_Lumi_1536_3X % Act Name: Dose response cell-based high-throughput screening assay to identify transcriptional activators of heat sho... aid1252.table aid1252.tbin
1253 186 Data Source: Columbia University Molecular Screening Center Source (MLSCN Center Name): Columbia University Molecular Screening Center Center Affiliation: Columbia University Molecular Screening Center Assay Provider: Drs. Tomas Mustelin and Lutz Tautz, Burnham Institute for Medical Research, La Jolla, CA Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: X01-MH077604-01 LYP, a lymphocyte specific protein tyrosine phosphatase that plays a critical regulatory role ... aid1253.table aid1253.tbin
1254 1195 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute (TSRI) Assay Provider: Claes Wahlestedt, TSRI Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number 1 R21 NS056950-01 Grant Proposal PI: Claes Wahlestedt External Assay ID: NPY-Y1_ANT_CNGC_1536_3X%INH Name: Cell-based high-throughput confirmation assay for antagonists of neuropeptide Y receptor Y1 (NPY-Y1) Description: Neuropeptide... aid1254.table aid1254.tbin
1255 1195 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Affiliation: The Scripps Research Institute, TSRI Assay Provider: Claes Wahlestedt, Scripps Florida Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number 1 R21 NS056950-01 Grant Proposal PI: Claes Wahlestedt External Assay ID: NPY-Y2_ANT_CNGC_1536_3X%INH (CS) Name: Counterscreen assay for antagonists of neuropeptide Y receptor Y1 (NPY-Y1): Cell-based high throughput assay to measure... aid1255.table aid1255.tbin
1256 707 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Affiliation: The Scripps Research Institute, TSRI Assay Provider: Claes Wahlestedt, Scripps Florida Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number 1 R21 NS056950-01 Grant Proposal PI: Claes Wahlestedt External Assay ID: NPY-Y1_ANT_CNGC_1536_3X%INH (CS) Name: Counterscreen assay for antagonists of neuropeptide Y receptor Y2 (NPY-Y2): Cell-based high throughput assay to measure... aid1256.table aid1256.tbin
1257 707 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Affiliation: The Scripps Research Institute, TSRI Assay Provider: Claes Wahlestedt, Scripps Florida Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number 1 R21 NS056950-01 Grant Proposal PI: Claes Wahlestedt External Assay ID: NPY-Y2_ANT_CNGC_1536_3X%INH Name: Cell-based high throughput confirmation assay for antagonists of neuropeptide Y receptor Y2 (NPY-Y2) Description: Neuropep... aid1257.table aid1257.tbin
1258 1122 Infection with Leishmania represents a major health concern in the developing world, with approximately 1.2 to 1.5 million cases reported annually and 350 million people (globally) at risk of infection. The limited number of available leishmaniasis treatments is complicated by (1) serious (toxic) side effects; and (2) an increase in chemoresistance. Therefore, the identification of new small molecules for the treatment of leishmaniasis is a critical. A simple, inexpensive and HTS amenable methodo... aid1258.table aid1258.tbin
1259 72 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute (TSRI) Assay Provider: Richard Morimoto, Northwestern University Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 5 R21 NS056337-02 Grant Proposal PI: Richard Morimoto External Assay ID: CYTOX_INH_LUMI_1536_CC50 Name: Cytotoxicity counterscreen assay for transcriptional activators of heat shock protein 70 (Hsp70) Description:... aid1259.table aid1259.tbin
1260 15 New Mexico Molecular Libraries Screening Center Screening Center PI: Larry A. Sklar, PhD Assay Provider/Institution: Todd A. Thompson, PhD/University of New Mexico Grant: NIH 1 X01 MH078937-01 Assay Implementation: Mark Haynes PhD, Mark Carter MS, Anna Waller PhD, Cristian Bologa PhD Background/Significance Prostate cancer contributes significantly to cancer-related deaths in the United States [Jemel et al, 2005]. Although there is no known etiology, prostatic adenocarcinomas likely develop fro... aid1260.table aid1260.tbin
1261 150 Yersinia pestis is the causal agent of the bubonic plague and, although modern antibiotics are extremely effective against the malady, the plague remains a threat in many areas in the world. Outbreaks of hundreds of cases still occur in Asia, Africa and South America and, in the United States cases are reported sporadically, mainly because of people handling infected animals or by being bitten by infected wild rodent fleas (http://www.cdc.gov). YopH (Yersinia outer protein H) is a protein essenti... aid1261.table aid1261.tbin
1262 1246 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute (TSRI) Assay Provider: David Frank Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 1 X01 MH079826-01 Grant Proposal PI: David Frank External Assay ID: STAT1_POT_LUMI_1536_3X%ACT Name: Confirmation cell-based high throughput screening assay to measure STAT1 activation Description: The signal transducer and activator of tr... aid1262.table aid1262.tbin
1263 199 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute (TSRI) Assay Provider: David Frank Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 1 X01 MH079826-01 Grant Proposal PI: David Frank External Assay ID: STAT1_INH_LUMI_1536_3X%INH Name:Confirmation cell-based high throughput screening assay to measure STAT1 inhibition Description: The signal transducer and activator of... aid1263.table aid1263.tbin
1264 66 The PLK1 HTS assay was developed and run at the University of Pittsburgh Molecular Screening Center (PMLSC) as part of the Molecular Library Screening Center Network (MLSCN)(1 X01 MH76330). Polo-like kinase 1 (Plk1) is a serine-threonine protein kinase that functions as a key regulator of mitosis/meiosis and cytokinesis (Barr et al., 2004). Numerous research studies have demonstrated that Plk1 gene expression is frequently up regulated in human cancers and carcinoma-derived cell lines (Simizu a... aid1264.table aid1264.tbin
1265 1215 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute (TSRI) Assay Provider: David Frank, Dana-Farber Cancer Institute Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 1 X01 MH079826-01 Grant Proposal PI: David Frank External Assay ID: STAT3_INH_LUMI_1536_3X%INH Name: Confirmation cell-based high throughput screening assay to measure STAT3 inhibition Description: The sign... aid1265.table aid1265.tbin
1266 28 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Number: None Assay Provider: Dr. John C. Reed, Sanford-Burnham Medical Research Institute The goal of this assay along with other related ones is to identify compounds that selectively inhibit one of the several known pathways that lead to NF-kB activation in mammalian cell... aid1266.table aid1266.tbin
1267 1197 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute (TSRI) Assay Provider: David Frank, Dana Farber Cancer Institute Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 1 X01 MH079826-01 Grant Proposal PI: David Frank External Assay ID: STAT3_POT_LUC_1536_3X%ACT Name: Confirmation cell-based high throughput screening assay to measure STAT3 activation Description: The signal t... aid1267.table aid1267.tbin
1268 158 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute, TSRI Assay Provider: Jeffery W Kelly, TSRI Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: MH078940-01 Grant Proposal PI: Jeffery W Kelly External Assay ID: Betaglucosidase_ACT_Fluor_384_EC50 Name: Dose response cell-based high throughput screening assay to identify enhancers of beta-glucosidase activity Description: Gauc... aid1268.table aid1268.tbin
1269 27 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Number: None Assay Provider: Dr. John C. Reed, Sanford-Burnham Medical Research Institute In vitro luciferase assay using purified luciferase was developed and performed to exclude the false positive hits which inhibit luciferase activity directly. The NIH library consisted... aid1269.table aid1269.tbin
1270 10 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Number: None Assay Provider: Dr. John C. Reed, Sanford-Burnham Medical Research Institute PMA/Ionomycin induced IL-8 production assay in HEK293 cells was developed and performed as an orthogonal assay to confirm the hits inhibiting antigen receptor induced NF-kB pathway. IL... aid1270.table aid1270.tbin
1271 1 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Number: None Assay Provider: Dr. John C. Reed, Sanford-Burnham Medical Research Institute Anti-cd3/cd28 induced IL-2 production assay in Jurkat T cells is developed and performed as an orthogonal assay to test whether compound SID 17450324 inhibits the antigen receptor induc... aid1271.table aid1271.tbin
1272 119 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Affiliation: The Scripps Research Institute, TSRI Assay Provider: Claes Wahlestedt, Scripps Florida Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number 1 R21 NS056950-01 Grant Proposal PI: Claes Wahlestedt External Assay ID: NPY-Y2_ANT_CNGC_1536_IC50 Name: Dose response cell-based screening assay for antagonists of neuropeptide Y receptor Y2 (NPY-Y2) Description: Neuropeptide... aid1272.table aid1272.tbin
1273 127982 Data Source: Columbia University Molecular Screening Center Source (MLSCN Center Name): Columbia University Molecular Screening Center Center Affiliation: Columbia University Molecular Screening Center Assay Provider: Fred Levine and Mark Mercola, The Burnham Institute at UCSD, La Jolla, CA Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: MH077630-01 The assay has been designed to screen for small molecule compounds that modulate insulin promoter activity (1). ... aid1273.table aid1273.tbin
1274 217639 NIH Molecular Libraries Screening Centers Network [MLSCN] Emory Chemical Biology Discovery Center in MLSCN Assay provider: Susan Smith, Emory University MLSCN Grant: MH083234-01 Oxidative stress (the excess production of cellular oxidizing substances) is a central component in many diseases. Reactive oxygen species (ROS) produce oxidative stress that plays a central role in inflammation in general, and in the tissue damage and abnormal cell growth and fibrosis associated with many diseases. ROS-... aid1274.table aid1274.tbin
1275 1027 NIH Molecular Libraries Screening Centers Network [MLSCN] Emory Chemical Biology Discovery Center in MLSCN Assay provider: Susan Smith, Emory University MLSCN Grant: MH083234-01 Oxidative stress (the excess production of cellular oxidizing substances) is a central component in many diseases. Reactive oxygen species (ROS) produce oxidative stress that plays a central role in inflammation in general, and in the tissue damage and abnormal cell growth and fibrosis associated with many diseases. ROS-... aid1275.table aid1275.tbin
1276 193457 Data Source: Columbia University Molecular Screening Center Source (MLSCN Center Name): Columbia University Molecular Screening Center Center Affiliation: Columbia University Molecular Screening Center Assay Provider: Jack T. Rogers Genetics and Ageing Research Unit; Psychiatry Department, Massachusetts General Hospital, Boston. Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: MH079854-01 Novel reagents that inhibit the Amyloid Precursor Protein (APP) translatio... aid1276.table aid1276.tbin
1277 63 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Affiliation: The Scripps Research Institute, TSRI Assay Provider: Claes Wahlestedt, Scripps Florida Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number 1 R21 NS056950-01 Grant Proposal PI: Claes Wahlestedt External Assay ID: NPY-Y1_ANT_CNGC_1536_IC50 Name: Dose response cell-based screening assay for antagonists of neuropeptide Y receptor Y1 (NPY-Y1) Description: Neuropeptide ... aid1277.table aid1277.tbin
1278 63 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Affiliation: The Scripps Research Institute, TSRI Assay Provider: Claes Wahlestedt, Scripps Florida Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number 1 R21 NS056950-01 Grant Proposal PI: Claes Wahlestedt External Assay ID: NPY-Y2_ANT_CNGC_1536_IC50 (CS) Name: Dose response counterscreen assay for neuropeptide Y receptor Y1 (NPY-Y1): Cell-based high throughput assay to measure ... aid1278.table aid1278.tbin
1279 119 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Affiliation: The Scripps Research Institute, TSRI Assay Provider: Claes Wahlestedt, Scripps Florida Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number 1 R21 NS056950-01 Grant Proposal PI: Claes Wahlestedt External Assay ID: NPY-Y1_ANT_CNGC_1536_IC50 (CS) Name: Dose response counterscreen for neuropeptide Y receptor Y2 (NPY-Y2): Cell-based high throughput assay to measure NPY-Y1... aid1279.table aid1279.tbin
1280 1 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Number: None Assay Provider: Dr. John C. Reed, Sanford-Burnham Medical Research Institute Description: the purpose of in vitro kinase assay is to test if the compound SID 17450324 is a direct inhibitor of PKC-beta. SID 17450324 was identified in earlier assays, 1266, 1269, 1... aid1280.table aid1280.tbin
1281 1 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Number: None Phorbol dibutryate(PDBu) induced IL-8 production in HEK293-NF-kB-luc stable cells is developed and performed for the purpose of confirming compound SID 17450324 as a possible candidate to selectively inhibit NF-kB activation. Compound SID 17450325 was identifi... aid1281.table aid1281.tbin
1282 1 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Number: None Assay Provider: Dr. John C. Reed, Sanford-Burnham Medical Research Institute The purpose of this vitro kinase assay is to test if the compound SID 17450324 is a direct inhibitor of IKK-beta. SID 17450324 was identified in earlier assays, 1266, 1269, 1270, 1287 ... aid1282.table aid1282.tbin
1283 1 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Number: None Assay Provider: Dr. John C. Reed, Sanford-Burnham Medical Research Institute the purpose of in vitro kinase assay is to test if the compound SID 17450324 is a direct inhibitor of PKC-theta. SID 17450324 was identified in earlier assays, 1266, 1269, 1270, 1287 a... aid1283.table aid1283.tbin
1284 362 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Affiliation: The Scripps Research Institute, TSRI Assay Provider: Scripps Florida Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number ML00111 Grant Proposal PI: Philip LoGrasso External Assay ID: JNK3_INH_TR-FRET_1536_IC50 Name: Dose response biochemical screening assay for inhibitors of c-Jun N-Terminal Kinase 3 (JNK3) Description: The c-Jun N-Terminal Kinases (JNK) are member... aid1284.table aid1284.tbin
1285 193771 Data Source: Columbia University Molecular Screening Center Source (MLSCN Center Name): Columbia University Molecular Screening Center Center Affiliation: Columbia University Molecular Screening Center Assay Provider: Jack T. Rogers Genetics and Ageing Research Unit; Psychiatry Department, Massachusetts General Hospital, Boston. Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: MH079854-01 Novel reagents that inhibit the Amyloid Precursor Protein (APP) translatio... aid1285.table aid1285.tbin
1286 86 The PKD HTS assay was developed and run at the University of Pittsburgh Molecular Screening Center (PMLSC) as part of the Molecular Library Screening Center Network (MLSCN)(1R03DA24898-01). Protein kinase D (PKD) is a novel family of serine/threonine kinases targeted by diacylglycerol. It regulates many fundamental cell functions including cell proliferation, survival, differentiation and protein trafficking, and plays important roles in pathological conditions such as cardiac hypertrophy and c... aid1286.table aid1286.tbin
1287 17 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Number: None Assay Provider: Dr. John C. Reed, Sanford-Burnham Medical Research Institute Different from that of PMA/Ionomycin which activates NF-kB via PKC, TNF induced NF-kB activation is through the TNF-receptor, TRADD and TRAF (Hsu, 1995). To exclude the hits which may a... aid1287.table aid1287.tbin
1288 773 Data Source: Columbia University Molecular Screening Center Source (MLSCN Center Name): Columbia University Molecular Screening Center Center Affiliation: Columbia University Molecular Screening Center Assay Provider: Thomas Mayer, Columbia University Molecular Screening Center Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 1R03MH076509-01 E Selectin HCS was developed and screened at the Columbia University Molecular Screening Center as part of the Molecular S... aid1288.table aid1288.tbin
1289 1 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Number: None Assay Provider: Dr. John C. Reed, Sanford-Burnham Medical Research Institute CD40 overexpresssion induced NF-kB luciferase in HEK293-NF-kB-Luc stable cells is developed and performed to test whether the compound SID 17450324 inhibits CD40 induced NF-kB pathway. ... aid1289.table aid1289.tbin
1290 1 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Number: None Assay Provider: Dr. John C. Reed, Sanford-Burnham Medical Research Institute NOD1 overexpresssion induced NF-kB luciferase in 293-NF-kB-Luc stable cells is developed and performed to test whether compound SID 17450324 inhibits NOD1 induced NF-kB pathway. SID 17... aid1290.table aid1290.tbin
1291 1 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Number: None Assay Provider: Dr. John C. Reed, Sanford-Burnham Medical Research Institute Doxorubincin induced NF-kB luciferase in HEK 293-NF-kB-Luc stable cells is developed and performed to test whether the compound SID 17450324 inhibits DNA damage induced NF-kB pathway. ... aid1291.table aid1291.tbin
1292 1 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Number: None Assay Provider: Dr. John C. Reed, Sanford-Burnham Medical Research Institute Retinoic acid induced NF-kB luciferase in HEK293-NF-kB-Luc stable cells is developed and performed to test whether the compound SID 17450324 inhibits RIG1 induced NF-kB pathway. SID 17... aid1292.table aid1292.tbin
1293 1 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Number: None Assay Provider: Dr. John C. Reed, Sanford-Burnham Medical Research Institute NOD2 overexpresssion induced NF-kB luciferase in 293-NF-kB-Luc stable cells is developed and performed to test whether compound SID 17450324 inhibits NOD2 induced NF-kB pathway. SID 17... aid1293.table aid1293.tbin
1294 1 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Number: None Assay Provider: Dr. John C. Reed, Sanford-Burnham Medical Research Institute CD4-TLR4 overexpresssion induced NF-kB luciferase in 293-NF-kB-Luc stable cells is developed and performed to test whether the compound SID 17450324 inhibits TLR4 induced NF-kB pathway.... aid1294.table aid1294.tbin
1295 1 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Number: None Assay Provider: Dr. John C. Reed, Sanford-Burnham Medical Research Institute PMA/Ionomycin-stimulated NF-kB DNA-binding activity assay was developed and performed to test whether the compound SID 17450324 affects PMA/Ionomycin induced NF-kB-DNA binding. SID 174... aid1295.table aid1295.tbin
1296 128716 Data Source: Columbia University Molecular Screening Center Source (MLSCN Center Name): Columbia University Molecular Screening Center Center Affiliation: Columbia University Molecular Screening Center Assay Provider: Fred Levine and Mark Mercola, The Burnham Institute at UCSD, La Jolla, CA Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: MH077630-01 The assay has been designed to screen for small molecule compounds that modulate insulin promoter activity (1). ... aid1296.table aid1296.tbin
1297 794 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute (TSRI) Assay Provider: Pat Griffin, TSRI Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 1 X01 MH079861-01 Grant Proposal PI: Pat Griffin, TSRI External Assay ID: PPARgSRC2_AG_TRFRET_1536_%ACT Name: Pimary biochemical High Throughput Screening assay for agonists of the steroid receptor coactivator 2 (SRC-2) recruitment by th... aid1297.table aid1297.tbin
1298 9532 Southern Research Molecular Libraries Screening Center (SRMLSC) Southern Research Institute (Birmingham, Alabama) NIH Molecular Libraries Screening Centers Network (MLSCN) Assay Provider: Dr. Eric Weiss, Northwestern University Award: 1R21NS056968-01 A screen for inhibitors of the budding yeast RAM network using a sensitized drug exporter inhibited strain. The RAM network is a novel and highly conserved signaling pathway involved in maintenance of polarized growth, cell proliferation, and contr... aid1298.table aid1298.tbin
1299 1 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Number: None Assay Provider: Dr. John C. Reed, Sanford-Burnham Medical Research Institute The cytotoxicity assay in different cell types was developed and performed to test the effect of compound SID 17450324 on cell viability in THP.1 cells. SID 17450324 was identified in ... aid1299.table aid1299.tbin
1300 794 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute (TSRI) Assay Provider: Pat Griffin, TSRI Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 1 X01 MH079861-01 Grant Proposal PI: Hugh Rosen, TSRI External Assay ID: PPARgSRC1_AG_TRFRET_1536_3X%ACT Name: Confirmation biochemical High Throughput Screening assay for agonists of the steroid receptor coactivator 1 (SRC-1) recruitment... aid1300.table aid1300.tbin
1301 794 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute, TSRI Assay Provider: Scripps Florida Network: Molecular Library Screening Center Network (MLSCN) Proposal Number: 1 X01 MH079861-01 http://molscreen.florida.scripps.edu/ External Assay ID: PPARgSRC3_AG_TRFRET_1536_3X%ACT Name: Confirmation biochemical High Throughput Screening assay for agonists of the steroid receptor coactivator 3 (SRC-3) recruitment by th... aid1301.table aid1301.tbin
1302 1 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Number: None Assay Provider: Dr. John C. Reed, Sanford-Burnham Medical Research Institute The cytotoxicity assay in different cell types was developed and performed to test the effect of compound SID 17450324 on cell viability in HeLa cells. SID 17450324 was identified in e... aid1302.table aid1302.tbin
1303 199 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute (TSRI) Assay Provider: Prem Subramaniam, The Scripps Research Institute Molecular Screening Center (SRIMSC) Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 1 X01 MH079826-01 Grant Proposal PI: David Frank http://molscreen.florida.scripps.edu/ External Assay ID: NFkB_INH_LUMI_1536_3X%INH (STAT1CS) Name: Counterscreen assay for... aid1303.table aid1303.tbin
1304 218117 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute (TSRI) Assay Provider: Claes Wahlestedt, TSRI Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number 1 R21 NS056950-01 Grant Proposal PI: Claes Wahlestedt External Assay ID: NPY-Y1_POT_CNGC_1536_%ACT Name: Primary cell-based high-throughput screening assay for potentiators or agonists of NPY-Y1 Description: Neuropeptide Y (NPY) is ... aid1304.table aid1304.tbin
1305 1244 Southern Research Molecular Libraries Screening Center (SRMLSC) Southern Research Institute (Birmingham, Alabama) NIH Molecular Libraries Screening Centers Network (MLSCN) Assay Provider: Dr. Eric Weiss, Northwestern University Award: 1R21NS056968-01 A screen for inhibitors of the budding yeast RAM network - Dose response with drug exporter sensitized control strain. The RAM network is a novel and highly conserved signaling pathway involved in maintenance of polarized growth, cell proliferatio... aid1305.table aid1305.tbin
1306 1246 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute (TSRI)http://molscreen.florida.scripps.edu/ Assay Provider: Prem Subramaniam, The Scripps Research Institute Molecular Screening Center (SRIMSC) Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 1 X01 MH079826-01 Grant Proposal PI: David Frank External Assay ID: NFkB_ ACT _LUMI_1536_3X%INH (STAT1CS) Name: Counterscreen assay f... aid1306.table aid1306.tbin
1307 1244 Southern Research Molecular Libraries Screening Center (SRMLSC) Southern Research Institute (Birmingham, Alabama) NIH Molecular Libraries Screening Centers Network (MLSCN) Assay Provider: Dr. Eric Weiss, Northwestern University Award: 1R21NS056968-01 A screen for inhibitors of the budding yeast RAM network - Dose response. The RAM network is a novel and highly conserved signaling pathway involved in maintenance of polarized growth, cell proliferation, and control of gene expression. The pathway... aid1307.table aid1307.tbin
1308 1215 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute (TSRI) http://molscreen.florida.scripps.edu Assay Provider: Prem Subramaniam, The Scripps Research Institute Molecular Screening Center (SRIMSC) Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 1 X01 MH079826-01 Grant Proposal PI: David Frank External Assay ID: NFkB_INH_LUMI_1536_3X%INH (STAT3CS) Name: Counterscreen assay for ... aid1308.table aid1308.tbin
1309 1197 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute (TSRI) Assay Provider: Prem Subramaniam, The Scripps Research Institute Molecular Screening Center (SRIMSC) Network: Molecular Library Screening Center Network (MLSCN) http://molscreen.florida.scripps.edu/ Grant Proposal Number: 1 X01 MH079826-01 Grant Proposal PI: David Frank External Assay ID: NFkB _ACT_LUMI_1536_3X%INH (STAT3 CS) Name: Counterscreen assay f... aid1309.table aid1309.tbin
1310 199 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute (TSRI) http://molscreen.florida.scripps.edu/ Assay Provider: David Frank Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 1 X01 MH079826-01 Grant Proposal PI: David Frank External Assay ID: STAT3_INH_LUMI_1536_3X%INH (CSRUN) Name: Counterscreen assay for STAT1 inhibitors: Cell-based high throughput assay to measure STAT3 inhib... aid1310.table aid1310.tbin
1311 3 University of New Mexico Assay Overview: Assay Support: NIH 1 X01 MH077613-01 Assay for disassembly of the 26S Proteasome PI: Dorota Skowyra, PhD Assay implementation: Chris Allen PhD, Peter Simons PhD, Anna Waller PhD, MaryAnn Osley PhD Target Team Leader for the Center: Larry Sklar (lsklar@salud.unm.edu) Assay Background and Significance: The 26S proteasome in a highly conserved ATP-dependent protease that plays a central role in the control of protein stability in all eukaryotic cells. It is... aid1311.table aid1311.tbin
1312 3 University of New Mexico Assay Overview: Assay Support: NIH 1 X01 MH077613-01 Assay for disassembly of the 26S Proteasome PI: Dorota Skowyra, PhD Assay implementation: Chris Allen PhD, Peter Simons PhD, Anna Waller PhD, MaryAnn Osley PhD Target Team Leader for the Center: Larry Sklar (lsklar@salud.unm.edu) Assay Background and Significance: The 26S proteasome in a highly conserved ATP-dependent protease that plays a central role in the control of protein stability in all eukaryotic cells. It is... aid1312.table aid1312.tbin
1313 3 University of New Mexico Assay Overview: Assay Support: NIH 1 X01 MH077613-01 Assay for disassembly of the 26S Proteasome PI: Dorota Skowyra, PhD Assay implementation: Chris Allen PhD, Peter Simons PhD, Anna Waller PhD, MaryAnn Osley PhD Target Team Leader for the Center: Larry Sklar (lsklar@salud.unm.edu) Assay Background and Significance: The 26S proteasome in a highly conserved ATP-dependent protease that plays a central role in the control of protein stability in all eukaryotic cells. It is... aid1313.table aid1313.tbin
1314 3 University of New Mexico Assay Overview: Assay Support: NIH 1 X01 MH077613-01 Assay for disassembly of the 26S Proteasome PI: Dorota Skowyra, PhD Assay implementation: Chris Allen PhD, Peter Simons PhD, Anna Waller PhD, MaryAnn Osley PhD Target Team Leader for the Center: Larry Sklar (lsklar@salud.unm.edu) Assay Background and Significance: The 26S proteasome in a highly conserved ATP-dependent protease that plays a central role in the control of protein stability in all eukaryotic cells. It is... aid1314.table aid1314.tbin
1315 86 The PKD HTS assay was developed and run at the University of Pittsburgh Molecular Screening Center (PMLSC) as part of the Molecular Library Screening Center Network (MLSCN)(1R03DA24898-01). Protein kinase D (PKD) is a novel family of serine/threonine kinases targeted by diacylglycerol. It regulates many fundamental cell functions including cell proliferation, survival, differentiation and protein trafficking, and plays important roles in pathological conditions such as cardiac hypertrophy and c... aid1315.table aid1315.tbin
1316 1246 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute (TSRI) http://molscreen.florida.scripps.edu/ Assay Provider: David Frank Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 1 X01 MH079826-01 Grant Proposal PI: David Frank External Assay ID: STAT3_ACT_LUMI_1536_3X%INH (CSRUN) Name: Counterscreen assay for STAT1 activators: Cell-based high throughput assay to measure STAT3 activ... aid1316.table aid1316.tbin
1317 1215 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute (TSRI) http://molscreen.florida.scripps.edu/ Assay Provider: David Frank, Dana-Farber Cancer Institute Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 1 X01 MH079826-01 Grant Proposal PI: David Frank External Assay ID: STAT1_INH_LUMI_1536_3X%INH (CSRUN) Name: Counterscreen assay for STAT3 inhibitors: Cell-based high throughpu... aid1317.table aid1317.tbin
1318 1197 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute (TSRI) http://molscreen.florida.scripps.edu/ Assay Provider: David Frank, Dana-Farber Cancer Institute Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 1 X01 MH079826-01 Grant Proposal PI: David Frank External Assay ID: STAT1_ACT_LUMI_1536_3X%INH (CSRUN) Name: Counterscreen assay for STAT3 activators: Cell-based high throughpu... aid1318.table aid1318.tbin
1319 349 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute (TSRI) Assay Provider: Pat Griffin, TSRI Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 1 X01 MH079861-01 Grant Proposal PI: Patrick Griffin, Scripps Florida External Assay ID: PPARgSRC1_AG_TRFRET_1536_EC50 Name: Dose response biochemical High Throughput Screening assay for agonists of the steroid receptor coactivator 1 (SR... aid1319.table aid1319.tbin
1320 834 University of New Mexico Assay Overview: Assay Support: NIH 1X01 MH079850-01 HTS to identify small molecule regulators of Bcl-2 family protein interactions PI: Larry Sklar, Ph.D. Assay Implementatiion: Peter Simons Ph.D, Susan Young MS, Anna Waller Ph.D, Mark Carter MS Dose Response Assay Background and Significance: One arm of apoptosis is regulated by the balance of anti-apoptotic and pro-apoptotic Bcl-2 family members. In humans, six genes have been identified that encode anti-apoptoti... aid1320.table aid1320.tbin
1321 218117 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute (TSRI) Assay Provider: Nagi Ayad, TSRI Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 1R21NS056991-01 Grant Proposal PI: Nagi Ayad, TSRI External Assay ID: Wee1_INH_LUMI_1536_%ACT Name: Primary Cell-based High Throughput Screening Assay for Inhibitors of Wee1 Degradation Description: The Cdc2/cyclinB protein complex plays ... aid1321.table aid1321.tbin
1322 834 University of New Mexico Assay Overview: Assay Support: NIH 1X01 MH079850-01 HTS to identify small molecule regulators of Bcl-2 family protein interactions PI: Larry Sklar, Ph.D. Assay Implementatiion: Peter Simons Ph.D, Susan Young MS, Anna Waller Ph.D, Mark Carter MS Dose Response Assay Background and Significance: One arm of apoptosis is regulated by the balance of anti-apoptotic and pro-apoptotic Bcl-2 family members. In humans, six genes have been identified that encode anti-apoptoti... aid1322.table aid1322.tbin
1323 349 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute (TSRI) Assay Provider: Pat Griffin, TSRI Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 1 X01 MH079861-01 Grant Proposal PI: Pat Griffin, TSRI External Assay ID: PPARgSRC2_AG_TRFRET_1536_EC50 Name: Dose response biochemical High Throughput Screening assay for agonists of the steroid receptor coactivator 2 (SRC-2) recruitmen... aid1323.table aid1323.tbin
1324 834 University of New Mexico Assay Overview: Assay Support: NIH 1X01 MH079850-01 HTS to identify small molecule regulators of Bcl-2 family protein interactions PI: Larry Sklar, Ph.D. Assay Implementatiion: Peter Simons Ph.D, Susan Young MS, Anna Waller Ph.D, Mark Carter MS Profiling Assay Background and Significance: One arm of apoptosis is regulated by the balance of anti-apoptotic and pro-apoptotic Bcl-2 family members. In humans, six genes have been identified that encode anti-apoptotic pro... aid1324.table aid1324.tbin
1325 194480 University of New Mexico Assay Overview: Assay Support: 1R03MH081228-01A1 High-throughput multiplex screening for ABC transporter inhibitors PI: Richard Larson MD, PhD Assay Development: Irena Ivnitski-Steele PhD Assay Implementation: Irena Ivnitski-Steele PhD, Terry Foutz, Anna Waller PhD, Mark Carter Target Team Leader for the Center: Bruce Edwards, PhD (BEdwards@salud.unm.edu Assay Background and Significance: The three major types of multidrug resistance (MDR) proteins in humans includ... aid1325.table aid1325.tbin
1326 193717 University of New Mexico Assay Overview: Assay Support: 1R03MH081228-01A1 High-throughput multiplex screening for ABC transporter inhibitors PI: Richard Larson MD, PhD Assay Development: Irena Ivnitski-Steele PhD Assay Implementation: Irena Ivnitski-Steele PhD, Terry Foutz, Anna Waller PhD, Mark Carter Target Team Leader for the Center: Bruce Edwards, PhD (BEdwards@salud.unm.edu Assay Background and Significance: The three major types of multidrug resistance (MDR) proteins in humans includ... aid1326.table aid1326.tbin
1327 834 University of New Mexico Assay Overview: Assay Support: NIH 1X01 MH079850-01 HTS to identify small molecule regulators of Bcl-2 family protein interactions PI: Larry Sklar, Ph.D. Assay Implementatiion: Peter Simons Ph.D, Susan Young MS, Anna Waller Ph.D, Mark Carter MS Dose Response Assay Background and Significance: One arm of apoptosis is regulated by the balance of anti-apoptotic and pro-apoptotic Bcl-2 family members. In humans, six genes have been identified that encode anti-apoptoti... aid1327.table aid1327.tbin
1328 834 University of New Mexico Assay Overview: Assay Support: NIH 1X01 MH079850-01 HTS to identify small molecule regulators of Bcl-2 family protein interactions PI: Larry Sklar, Ph.D. Assay Implementatiion: Peter Simons Ph.D, Susan Young MS, Anna Waller Ph.D, Mark Carter MS Dose Response Assay Background and Significance: One arm of apoptosis is regulated by the balance of anti-apoptotic and pro-apoptotic Bcl-2 family members. In humans, six genes have been identified that encode anti-apoptotic... aid1328.table aid1328.tbin
1329 834 University of New Mexico Assay Overview: Assay Support: NIH 1X01 MH079850-01 HTS to identify small molecule regulators of Bcl-2 family protein interactions PI: Larry Sklar, Ph.D. Assay Implementatiion: Peter Simons Ph.D, Susan Young MS, Anna Waller Ph.D, Mark Carter MS Dose Response Assay Background and Significance: One arm of apoptosis is regulated by the balance of anti-apoptotic and pro-apoptotic Bcl-2 family members. In humans, six genes have been identified that encode anti-apoptoti... aid1329.table aid1329.tbin
1330 834 University of New Mexico Assay Overview: Assay Support: NIH 1X01 MH079850-01 HTS to identify small molecule regulators of Bcl-2 family protein interactions PI: Larry Sklar, Ph.D. Assay Implementatiion: Peter Simons Ph.D, Susan Young MS, Anna Waller Ph.D, Mark Carter MS Dose Response Assay Background and Significance: One arm of apoptosis is regulated by the balance of anti-apoptotic and pro-apoptotic Bcl-2 family members. In humans, six genes have been identified that encode anti-apoptoti... aid1330.table aid1330.tbin
1331 349 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center http://molscreen.florida.scripps.edu/ Center Affiliation: The Scripps Research Institute, TSRI Assay Provider: Pat Griffin, TSRI Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 1 X01 MH079861-01 Grant Proposal PI: Pat Griffin, TSRI External Assay ID: PPARgSRC3_AG_TRFRET_1536_EC50 Name: Dose response biochemical High Throughput Screening assay for agonists of the steroid rec... aid1331.table aid1331.tbin
1332 1118 Southern Research Institute (Birmingham, Alabama) Award: N01 AI 15449 "Microbiological Drug Screening" E. Lucile White, P.I. Mycobacterium tuberculosis (Mtb) is a notorious pathogen whose increasing resistance to antibiotics and heightened lethality in combination with AIDS makes it a major health concern worldwide. The World Health Organization (WHO) estimates that one-third of the world's population is infected with Mtb; eight million people worldwide develop tuberculosis annually while nearly... aid1332.table aid1332.tbin
1333 1269 University of New Mexico Assay Overview: Assay Support: NIH I RO3 MH081231-01 HTS to identify specific small molecule inhibitors of Ras and Ras-related GTPases PI: Angela Wandinger-Ness, Ph.D. Co-PI: Larry Sklar, Ph.D. Assay Development: Zurab Surviladze, Ph.D. Assay Implementation: Zurab Surviladze, Danuta Wlodek, Terry Foutz, Mark Carter, Anna Waller Target Team Leader for the Center: Larry Sklar (lsklar@salud.unm.edu) Dose Response Assay Background and Significance: Ras and related small mo... aid1333.table aid1333.tbin
1334 1270 University of New Mexico Assay Overview: Assay Support: NIH I RO3 MH081231-01 HTS to identify specific small molecule inhibitors of Ras and Ras-related GTPases PI: Angela Wandinger-Ness, Ph.D. Co-PI: Larry Sklar, Ph.D. Assay Development: Zurab Surviladze, Ph.D. Assay Implementation: Zurab Surviladze, Danuta Wlodek, Terry Foutz, Mark Carter, Anna Waller Target Team Leader for the Center: Larry Sklar (lsklar@salud.unm.edu) Dose Response Assay Background and Significance: Ras and related small mo... aid1334.table aid1334.tbin
1335 1270 University of New Mexico Assay Overview: Assay Support: NIH I RO3 MH081231-01 HTS to identify specific small molecule inhibitors of Ras and Ras-related GTPases PI: Angela Wandinger-Ness, Ph.D. Co-PI: Larry Sklar, Ph.D. Assay Development: Zurab Surviladze, Ph.D. Assay Implementation: Zurab Surviladze, Danuta Wlodek, Terry Foutz, Mark Carter, Anna Waller Target Team Leader for the Center: Larry Sklar (lsklar@salud.unm.edu) Dose Response Assay Background and Significance: Ras and related small mo... aid1335.table aid1335.tbin
1336 1270 University of New Mexico Assay Overview: Assay Support: NIH I RO3 MH081231-01 HTS to identify specific small molecule inhibitors of Ras and Ras-related GTPases PI: Angela Wandinger-Ness, Ph.D. Co-PI: Larry Sklar, Ph.D. Assay Development: Zurab Surviladze, Ph.D. Assay Implementation: Zurab Surviladze, Danuta Wlodek, Terry Foutz, Mark Carter, Anna Waller Target Team Leader for the Center: Larry Sklar (lsklar@salud.unm.edu) Dose Response Assay Background and Significance: Ras and related small mo... aid1336.table aid1336.tbin
1337 1270 University of New Mexico Assay Overview: Assay Support: NIH I RO3 MH081231-01 HTS to identify specific small molecule inhibitors of Ras and Ras-related GTPases PI: Angela Wandinger-Ness, Ph.D. Co-PI: Larry Sklar, Ph.D. Assay Development: Zurab Surviladze, Ph.D. Assay Implementation: Zurab Surviladze, Danuta Wlodek, Terry Foutz, Mark Carter, Anna Waller Target Team Leader for the Center: Larry Sklar (lsklar@salud.unm.edu) Dose Response Assay Background and Significance: Ras and related small mo... aid1337.table aid1337.tbin
1338 12 Data Source: Columbia University Molecular Screening Center Source (MLSCN Center Name): Columbia University Molecular Screening Center Center Affiliation: Columbia University Molecular Screening Center Assay Provider: Drs. Tomas Mustelin and Lutz Tautz, Burnham Institute for Medical Research, La Jolla, CA Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: X01-MH077604-01 LYP, a lymphocyte specific protein tyrosine phosphatase that plays a critical regulatory role... aid1338.table aid1338.tbin
1339 1289 University of New Mexico Assay Overview: Assay Support: NIH I RO3 MH081231-01 HTS to identify specific small molecule inhibitors of Ras and Ras-related GTPases PI: Angela Wandinger-Ness, Ph.D. Co-PI: Larry Sklar, Ph.D. Assay Development: Zurab Surviladze, Ph.D. Assay Implementation: Zurab Surviladze, Danuta Wlodek, Terry Foutz, Mark Carter, Anna Waller Target Team Leader for the Center: Larry Sklar (lsklar@salud.unm.edu) Dose Response Assay Background and Significance: Ras and related small mo... aid1339.table aid1339.tbin
1340 1289 University of New Mexico Assay Overview: Assay Support: NIH I RO3 MH081231-01 HTS to identify specific small molecule inhibitors of Ras and Ras-related GTPases PI: Angela Wandinger-Ness, Ph.D. Co-PI: Larry Sklar, Ph.D. Assay Development: Zurab Surviladze, Ph.D. Assay Implementation: Zurab Surviladze, Danuta Wlodek, Terry Foutz, Mark Carter, Anna Waller Target Team Leader for the Center: Larry Sklar (lsklar@salud.unm.edu) Dose Response Assay Background and Significance: Ras and related small mo... aid1340.table aid1340.tbin
1341 1270 University of New Mexico Assay Overview: Assay Support: NIH I RO3 MH081231-01 HTS to identify specific small molecule inhibitors of Ras and Ras-related GTPases PI: Angela Wandinger-Ness, Ph.D. Co-PI: Larry Sklar, Ph.D. Assay Development: Zurab Surviladze, Ph.D. Assay Implementation: Zurab Surviladze, Danuta Wlodek, Terry Foutz, Mark Carter, Anna Waller Target Team Leader for the Center: Larry Sklar (lsklar@salud.unm.edu) Dose Response Assay Background and Significance: Ras and related small mo... aid1341.table aid1341.tbin
1342 18 The PKD HTS assay was developed and run at the University of Pittsburgh Molecular Screening Center (PMLSC) as part of the Molecular Library Screening Center Network (MLSCN)(1R03DA24898-01). Protein kinase D (PKD) is a novel family of serine/threonine kinases targeted by diacylglycerol. It regulates many fundamental cell functions including cell proliferation, survival, differentiation and protein trafficking, and plays important roles in pathological conditions such as cardiac hypertrophy and c... aid1342.table aid1342.tbin
1343 11 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Proposal Number: 1R03DA024887-01 Assay Provider: Dr. John C. Reed, Sanford-Burnham Medical Research Institute ER stress is a cellular response initiated by unfolded protein accumulation in the Endoplasmic Reticulum (ER). Prolonged ER stress induces cell death with a form of a... aid1343.table aid1343.tbin
1344 11 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Proposal Number: 1R03DA024887-01 Assay Provider: Dr. John C. Reed, Sanford-Burnham Medical Research Institute ER stress is a cellular response initiated by unfolded protein accumulation in the Endoplasmic Reticulum (ER). Prolonged ER stress induces cell death with a form of... aid1344.table aid1344.tbin
1345 11 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Proposal Number: 1R03DA024887-01 Assay Provider: Dr. John C. Reed, Sanford-Burnham Medical Research Institute ER stress is a cellular response initiated by unfolded protein accumulation in the Endoplasmic Reticulum (ER). Prolonged ER stress induces cell death with a form of a... aid1345.table aid1345.tbin
1346 11 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Proposal Number: 1R03DA024887-01 Assay Provider: Dr. John C. Reed, Sanford-Burnham Medical Research Institute ER stress is a cellular response initiated by unfolded protein accumulation in the Endoplasmic Reticulum (ER). Prolonged ER stress induces cell death with a form of ... aid1346.table aid1346.tbin
1347 11 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Proposal Number: 1R03DA024887-01 Assay Provider: Dr. John C. Reed, Sanford-Burnham Medical Research Institute ER stress is a cellular response initiated by unfolded protein accumulation in the Endoplasmic Reticulum (ER). Prolonged ER stress induces cell death with a form of a... aid1347.table aid1347.tbin
1348 11 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Proposal Number: 1R03DA024887-01 Assay Provider: Dr. John C. Reed, Sanford-Burnham Medical Research Institute ER stress is a cellular response initiated by unfolded protein accumulation in the Endoplasmic Reticulum (ER). Prolonged ER stress induces cell death with a form of a... aid1348.table aid1348.tbin
1349 11 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Proposal Number: 1R03DA024887-01 Assay Provider: Dr. John C. Reed, Sanford-Burnham Medical Research Institute ER stress is a cellular response initiated by unfolded protein accumulation in the Endoplasmic Reticulum (ER). Prolonged ER stress induces cell death with a form of a... aid1349.table aid1349.tbin
1350 11 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Proposal Number: 1R03DA024887-01 Assay Provider: Dr. John C. Reed, Sanford-Burnham Medical Research Institute ER stress is a cellular response initiated by unfolded protein accumulation in the Endoplasmic Reticulum (ER). Prolonged ER stress induces cell death with a form of a... aid1350.table aid1350.tbin
1351 11 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Proposal Number: 1R03DA024887-01 Assay Provider: Dr. John C. Reed, Sanford-Burnham Medical Research Institute ER stress is a cellular response initiated by unfolded protein accumulation in the Endoplasmic Reticulum (ER). Prolonged ER stress induces cell death with a form of a... aid1351.table aid1351.tbin
1352 11 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Proposal Number: 1R03DA024887-01 Assay Provider: Dr. John C. Reed, Sanford-Burnham Medical Research Institute ER stress is a cellular response initiated by unfolded protein accumulation in the Endoplasmic Reticulum (ER). Prolonged ER stress induces cell death with a form of ... aid1352.table aid1352.tbin
1353 11 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Proposal Number: 1R03DA024887-01 Assay Provider: Dr. John C. Reed, Sanford-Burnham Medical Research Institute ER stress is a cellular response initiated by unfolded protein accumulation in the Endoplasmic Reticulum (ER). Prolonged ER stress induces cell death with a form of ... aid1353.table aid1353.tbin
1354 11 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Proposal Number: 1R03DA024887-01 Assay Provider: Dr. John C. Reed, Sanford-Burnham Medical Research Institute ER stress is a cellular response initiated by unfolded protein accumulation in the Endoplasmic Reticulum (ER). Prolonged ER stress induces cell death with a form of a... aid1354.table aid1354.tbin
1355 11 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Proposal Number: 1R03DA024887-01 Assay Provider: Dr. John C. Reed, Sanford-Burnham Medical Research Institute ER stress is a cellular response initiated by unfolded protein accumulation in the Endoplasmic Reticulum (ER). Prolonged ER stress induces cell death with a form of a... aid1355.table aid1355.tbin
1356 11 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Proposal Number: 1R03DA024887-01 Assay Provider: Dr. John C. Reed, Sanford-Burnham Medical Research Institute ER stress is a cellular response initiated by unfolded protein accumulation in the Endoplasmic Reticulum (ER). Prolonged ER stress induces cell death with a form of ... aid1356.table aid1356.tbin
1357 11 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Proposal Number: 1R03DA024887-01 Assay Provider: Dr. John C. Reed, Sanford-Burnham Medical Research Institute ER stress is a cellular response initiated by unfolded protein accumulation in the Endoplasmic Reticulum (ER). Prolonged ER stress induces cell death with a form of a... aid1357.table aid1357.tbin
1358 11 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Proposal Number: 1R03DA024887-01 Assay Provider: Dr. John C. Reed, Sanford-Burnham Medical Research Institute ER stress is a cellular response initiated by unfolded protein accumulation in the Endoplasmic Reticulum (ER). Prolonged ER stress induces cell death with a form of a... aid1358.table aid1358.tbin
1359 218117 Source (MLPCN Center Name): The Scripps Research Institute Molecular Screening Center(SRIMSC) Center Affiliation: The Scripps Research Institute (TSRI) Assay Provider: Patricia McDonald, Scripps Florida Network: Molecular Libraries Probe Production Centers Network (MLPCN) Grant Proposal Number 1 R21 NS056950-01 Grant Proposal PI: Claes Wahlestedt External Assay ID: NPY-Y2_POT_CNGC_1536_%ACT Name: Primary cell-based high-throughput screening assay for potentiators or agonists of NPY-Y2 Descript... aid1359.table aid1359.tbin
1360 11 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Proposal Number: 1R03DA024887-01 Assay Provider: Dr. John C. Reed, Sanford-Burnham Medical Research Institute ER stress is a cellular response initiated by unfolded protein accumulation in the Endoplasmic Reticulum (ER). Prolonged ER stress induces cell death with a form of ... aid1360.table aid1360.tbin
1361 1146 Southern Research Molecular Libraries Screening Center (SRMLSC) Southern Research Institute (Birmingham, Alabama) NIH Molecular Libraries Screening Centers Network (MLSCN) Assay Provider: Dr. Jodi M. Nunnari, University of California, Davis Award: R03 MH081279-01 Screening for compounds that inhibit mitochondrial fusion using a yeast model system as a primary screening tool - confirmatory screen. Mitochondria are essential, double-membraned organelles that perform a myriad of tasks within cell... aid1361.table aid1361.tbin
1362 194235 Southern Research Molecular Libraries Screening Center (SRMLSC) Southern Research Institute (Birmingham, Alabama) NIH Molecular Libraries Screening Centers Network (MLSCN) Assay Provider: Dr. Jodi M. Nunnari, University of California, Davis Award: R03 MH081279-01 Screening for compounds that inhibit mitochondrial fusion using a yeast model system as a primary screening tool Mitochondria are essential, double-membraned organelles that perform a myriad of tasks within cells. Unlike their bacteri... aid1362.table aid1362.tbin
1363 2 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute, TSRI Assay Provider: Germana Sanna, TSRI Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 1 R03 MH076533-01 Grant Proposal PI: Germana Sanna External Assay ID: S1P1_ANT_RAD_96_IC50 Name: Cell-membrane dose response assay to identify antagonists of the Sphingosine 1-Phosphate receptor 1 (S1P1) Description: Sphingosine 1-phos... aid1363.table aid1363.tbin
1364 1 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Number: None Assay Provider: Dr. John C. Reed, Sanford-Burnham Medical Research Institute Anti-cd3/cd28 induced T cell proliferation of mouse primary lymphocytes was developed and performed to test whether the compound SID 17450324 inhibits primary T cell proliferation. SID ... aid1364.table aid1364.tbin
1365 1 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Number: None Assay Provider: Dr. John C. Reed, Sanford-Burnham Medical Research Institute An anti-IgM induced B cell proliferation of mouse primary lymphocytes assay was developed and performed to test whether the compound SID 17450324 inhibits primary B cell proliferation. ... aid1365.table aid1365.tbin
1366 1 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Number: None Assay Provider: Dr. John C. Reed, Sanford-Burnham Medical Research Institute PMA/Ionomycin induced NF-kB luciferase in MCF7 cells is developed and performed to test whether the compound SID 17450324 inhibits PMA/Ionomycin induced NF-kB in other cell types. SID 1... aid1366.table aid1366.tbin
1367 1 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Number: None Assay Provider: Dr. John C. Reed, Sanford-Burnham Medical Research Institute The cytotoxicity assay in different cell types was developed and performed to test the effect of compound SID 17450324 on cell viability in Jurkat T cells. SID 17450324 was identified i... aid1367.table aid1367.tbin
1368 27 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Number: None Assay Provider: Dr. John C. Reed, Sanford-Burnham Medical Research Institute A cytotoxicity assay in different cell types was developed and performed to test the effect of the compounds on cell viability. A total of 27 compounds were tested in this assay in HEK2... aid1368.table aid1368.tbin
1369 1 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Number: None Assay Provider: Dr. John C. Reed, Sanford-Burnham Medical Research Institute LPS-induced NF-kB assay in THP.1 cells was developed and performed to test whether the hit SID 17450324 inhibits LPS induced NF-kB pathway. Lipopolysaccharide (LPS) induced Toll-like re... aid1369.table aid1369.tbin
1370 1 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Number: None Assay Provider: Dr. John C. Reed, Sanford-Burnham Medical Research Institute GM-Tri-DAP induced IL-8 production assay in MCF-7 cells is developed and performed to test if the hit SID 17450324 affects NOD1 induced NF-kB pathway. SID 17450324 was identified in ea... aid1370.table aid1370.tbin
1371 1 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Number: None Assay Provider: Dr. John C. Reed, Sanford-Burnham Medical Research Institute A PMA/Ionomycin induced NF-kB luciferase in HEK293T cells assay is developed and performed to test whether the compound SID 17450324 inhibits PMA/Ionomycin induced NF-kB in HEK293T cell... aid1371.table aid1371.tbin
1372 1 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Number: None Assay Provider: Dr. John C. Reed, Sanford-Burnham Medical Research Institute A cytotoxicity assay for different cell types was developed and performed to test the effect of compound SID 17450324 on cell viability in HEK293T cells. SID 17450324 was identified in... aid1372.table aid1372.tbin
1373 1 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Number: None Assay Provider: Dr. John C. Reed, Sanford-Burnham Medical Research Institute A cytotoxicity assay in different cell types was developed used to test the effect of compound SID 17450324 on cell viability in MCF7 cells. SID 17450324 was identified in earlier assa... aid1373.table aid1373.tbin
1374 1 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Number: None Assay Provider: Dr. John C. Reed, Sanford-Burnham Medical Research Institute PMA/Ionomycin induced NF-kB luciferase in HeLa cells is developed and performed to test whether the compound SID 17450324 inhibits PMA/Ionomycin induced NF-kB in other cell types. SID ... aid1374.table aid1374.tbin
1375 1 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Number: None Assay Provider: Dr. John C. Reed, Sanford-Burnham Medical Research Institute PMA/Ionomycin induced NF-kB luciferase in PPC-1 cells is developed and performed to test whether compound SID 17450324 inhibits PMA/Ionomycin induced NF-kB in other cell types. SID 174... aid1375.table aid1375.tbin
1376 216162 Southern Research Molecular Libraries Screening Center (SRMLSC) Southern Research Institute (Birmingham, Alabama) NIH Molecular Libraries Screening Centers Network (MLSCN) Assay Provider: Dr. Michael McNeil, Colorado State University, Fort Collins, CO Mycobacterial Glucosamine-1-phosphate acetyl transferase GlmU) is a bi-functional enzyme involved in peptidoglycan synthesis by converting glucosamine-1-phosphate to UDP-N- acetylglucosamine in two distinct steps. The first step catalyzes the... aid1376.table aid1376.tbin
1377 217210 A HTS to identify inhibitors of zVAD Induced Cell Death in L929 Cells performed by the PMLSC in the University of Pittsburgh Drug Discovery Institute. Excerpts from the MH81266 Application - Dr. Junying Yuan, Harvard University. Necrosis in physiological and pathological conditions. Necrosis is a caspase-independent cell death marked by a rapid loss of plasma membrane integrity, organelle swelling and mitochondrial dysfunction, and lacking typical features of apoptosis such as internucleosomal DN... aid1377.table aid1377.tbin
1378 18 The PKD HTS assay was developed and run at the University of Pittsburgh Molecular Screening Center (PMLSC) as part of the Molecular Library Screening Center Network (MLSCN)(1R03DA24898-01). Protein kinase D (PKD) is a novel family of serine/threonine kinases targeted by diacylglycerol. It regulates many fundamental cell functions including cell proliferation, survival, differentiation and protein trafficking, and plays important roles in pathological conditions such as cardiac hypertrophy and c... aid1378.table aid1378.tbin
1379 201160 NCGC Assay Overview: NIH Molecular Libraries Screening Centers Network [MLSCN] NIH Chemical Genomics Center [NCGC] MLSCN Grant: None Luciferase (Kinase-Glo, Promega Corporation) was assayed for its ability to generate light using ATP and luciferin as substrates. The ATP concentration in the assay (10 uM) was within the linear range of enzyme activity for the assay conditions used. aid1379.table aid1379.tbin
1380 18 The PKD HTS assay was developed and run at the University of Pittsburgh Molecular Screening Center (PMLSC) as part of the Molecular Library Screening Center Network (MLSCN)(1R03DA24898-01). Protein kinase D (PKD) is a novel family of serine/threonine kinases targeted by diacylglycerol. It regulates many fundamental cell functions including cell proliferation, survival, differentiation and protein trafficking, and plays important roles in pathological conditions such as cardiac hypertrophy and c... aid1380.table aid1380.tbin
1381 220015 Excerpts from the R21NS057026 Application - Dr. Billy Day University of Pittsburgh. Cytoplasmic dynein is the molecular motor that carries cargo to the minus ends of microtubules (MTs) (e.g., from the cytoplasm to the nucleus), and provides the mechancial force for many other important fuctions, including nuclear envelope breakdown and sister chromatid exchange at mitosis. Unlike the numerous MT plus end-directed molecular motors, the kinesins, no specific small molecule inhibitors of dynein are... aid1381.table aid1381.tbin
1382 24 NCGC Assay Overview: NIH Molecular Libraries Screening Centers Network [MLSCN] NIH Chemical Genomics Center [NCGC] MLSCN Grant: XO1-MH078932-01 Beta-glucocerebrosidase catalyzes the hydrolysis of beta-glucocerebroside to glucose and ceramide. The inherited deficiency of beta-glucocerebrosidase results in Gaucher disease, which is characterized by a wide variety of symptoms including hepatosplenomegaly, anemia, thrombocytopenia, bony lesions and bone marrow infiltration with characteristic stora... aid1382.table aid1382.tbin
1383 12 The PKD HTS assay was developed and run at the University of Pittsburgh Molecular Screening Center (PMLSC) as part of the Molecular Library Screening Center Network (MLSCN)(1R03DA24898-01). Protein kinase D (PKD) is a novel family of serine/threonine kinases targeted by diacylglycerol. It regulates many fundamental cell functions including cell proliferation, survival, differentiation and protein trafficking, and plays important roles in pathological conditions such as cardiac hypertrophy and c... aid1383.table aid1383.tbin
1384 1 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Number: None Assay Provider: Dr. John C. Reed, Sanford-Burnham Medical Research Institute One of the cellular pathways leading to activation of NF-kB-family transcription factors, participating in host-defense, immunity, inflammation, and cancer is a pathway activated by ant... aid1384.table aid1384.tbin
1385 198479 Project Title: A screen for modulators of human Rad51, a key DNA repair protein Application Number: MH084119 Assay Submitter: Dr. Alex Mazin Submitter Institution: Drexel University Screening Center Name: Penn Center for Molecular Discovery (PCMD) Principal Investigator of Screening Center: Scott Diamond Ionizing radiation (IR) and inter-strand cross-linking agents (ICL) induce DNA double-stranded breaks (DSB). DSB are the most harmful type of DNA damage, which cause genome instability, can... aid1385.table aid1385.tbin
1386 1 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Proposal Number: 1R03DA024887-01 Assay Provider: Dr. John C. Reed, Sanford-Burnham Medical Research Institute ER stress is a cellular response initiated by unfolded protein accumulation in the Endoplasmic Reticulum (ER). Prolonged ER stress induces cell death with a form of ... aid1386.table aid1386.tbin
1387 2 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Proposal Number: 1R03DA024887-01 Assay Provider: Dr. John C. Reed, Sanford-Burnham Medical Research Institute ER stress is a cellular response initiated by unfolded protein accumulation in the Endoplasmic Reticulum (ER). Prolonged ER stress induces cell death with a form of ... aid1387.table aid1387.tbin
1388 11 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Proposal Number: 1R03DA024887-01 Assay Provider: Dr. John C. Reed, Sanford-Burnham Medical Research Institute ER stress is a cellular response initiated by unfolded protein accumulation in the Endoplasmic Reticulum (ER). Prolonged ER stress induces cell death with a form of ... aid1388.table aid1388.tbin
1389 11 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Proposal Number: 1R03DA024887-01 Assay Provider: Dr. John C. Reed, Sanford-Burnham Medical Research Institute ER stress is a cellular response initiated by unfolded protein accumulation in the Endoplasmic Reticulum (ER). Prolonged ER stress induces cell death with a form of ... aid1389.table aid1389.tbin
1390 11 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Proposal Number: 1R03DA024887-01 Assay Provider: Dr. John C. Reed, Sanford-Burnham Medical Research Institute ER stress is a cellular response initiated by unfolded protein accumulation in the Endoplasmic Reticulum (ER). Prolonged ER stress induces cell death with a form of ... aid1390.table aid1390.tbin
1391 2 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Proposal Number: 1R03DA024887-01 Assay Provider: Dr. John C. Reed, Sanford-Burnham Medical Research Institute ER stress is a cellular response initiated by unfolded protein accumulation in the Endoplasmic Reticulum (ER). Prolonged ER stress induces cell death with a form of ... aid1391.table aid1391.tbin
1392 52 Screening Center: Penn Center for Molecular Discovery Center Affiliation: University of Pennsylvania Network: Molecular Library Screening Center Network (MLSCN) Assay Provider: Arkady Mustaev, Public Health Research Institute, Newark, NJ Grant number: MH076325-01 DNA-directed RNA polymerase (EC 2.7.7.6) is responsible for bacterial RNA synthesis and as such is essential for bacterial gene expression. Owing to its central role in DNA transcription, the enzyme RNA polymerase is the target of vario... aid1392.table aid1392.tbin
1393 21 NCGC Assay Overview: NIH Molecular Libraries Screening Centers Network [MLSCN] NIH Chemical Genomics Center [NCGC] MLSCN Grant: XO1-MH078932-01 Beta-glucocerebrosidase catalyzes the hydrolysis of beta-glucocerebroside to glucose and ceramide. The inherited deficiency of beta-glucocerebrosidase results in Gaucher disease, which is characterized by a wide variety of symptoms including hepatosplenomegaly, anemia, thrombocytopenia, bony lesions and bone marrow infiltration with characteristic stora... aid1393.table aid1393.tbin
1394 200 Molecular Library Screening Center Network (MLSCN) Penn Center for Molecular Discovery (PCMD) Assay Provider: Brent Stockwell, Columbia University MLSCN Grant: R03MH082369-01 The E3 ligases are involved in regulating other proteins by covalent ligation to the 76 amino acid protein ubiquitin. This post-translational modification can result in altered conformation, altered activity, or degradation of the substrate protein. Thus, E3 ligases are effectors of a major means of post-translational modif... aid1394.table aid1394.tbin
1395 1 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Number: 1R03 DA024887-01 Assay Provider: Dr. John C. Reed, Sanford-Burnham Medical Research Institute The endoplasmic reticulum (ER) fulfills multiple cellular functions (reviewed in [1-7]). Myriad types of disturbances cause accumulation of unfolded proteins in the ER, trig... aid1395.table aid1395.tbin
1396 53 Source (MLPCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute (TSRI) Assay Provider: David Frank, Dana Farber Cancer Institute Network: Molecular Libraries Probe Production Centers Network (MLPCN) Grant Proposal Number: 1 X01 MH079826-01 Grant Proposal PI: David Frank, Dana Farber Cancer Institute External Assay ID: STAT1_INH_LUMI_1536_IC50 Name: Dose response cell-based assay to measure STAT1 inhibition Descripti... aid1396.table aid1396.tbin
1397 107 Source (MLPCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute (TSRI) Assay Provider: David Frank, Dana Farber Cancer Institute Network: Molecular Libraries Probe Production Centers Network (MLPCN) Grant Proposal Number: 1 X01 MH079826-01 Grant Proposal PI: David Frank, Dana Farber Cancer Institute External Assay ID: STAT1_ACT_LUMI_1536_EC50 Name: Dose response cell-based assay to measure STAT1 activation Descript... aid1397.table aid1397.tbin
1398 122 Source (MLPCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute (TSRI) Assay Provider: David Frank, Dana Farber Cancer Institute Network: Molecular Libraries Probe Production Centers Network (MLPCN) Grant Proposal Number: 1 X01 MH079826-01 Grant Proposal PI: David Frank, Dana Farber Cancer Institute External Assay ID: STAT3_ACT_LUMI_1536_EC50 Name: Dose response cell-based assay to measure STAT3 activation Descriptio... aid1398.table aid1398.tbin
1399 118 Source (MLPCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute (TSRI) Assay Provider: David Frank, Dana Farber Cancer Institute Network: Molecular Libraries Probe Production Centers Network (MLPCN) Grant Proposal Number: 1 X01 MH079826-01 Grant Proposal PI: David Frank, Dana Farber Cancer Institute External Assay ID: STAT3_INH_LUMI_1536_IC50 Name: Dose response cell-based assay to measure STAT3 inhibition Descrip... aid1399.table aid1399.tbin
1400 9 NCGC Assay Overview: NIH Molecular Libraries Screening Centers Network [MLSCN] NIH Chemical Genomics Center [NCGC] MLSCN Grant: XO1-MH077625-01 Hsp90 (heat shock protein 90) is the essential molecular chaperone and it accounts for 1-2% of all cytosolic proteins and is critical for the activity of diverse cellular proteins that are involved in a variety of cellular processes, including development, cell cycle, and steroid hormone signaling. Its client proteins include signaling kinases such as I... aid1400.table aid1400.tbin
1401 126 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] MLSCN Grant: 1 X01 MH080680-01 PI Name: Dr. Marvin Gershengorn, NIH NCGC Assay Overview: Thyroid Stimulating Hormone Receptor TSH is an alpha/beta heterodimeric glycoprotein hormone secreted from the anterior pituitary gland which belongs to the glycoprotein hormone family. The actions of TSH are mediated by a seven-transmembrane receptor, which upon TSH binding couples preferentially to the G-alpha (... aid1401.table aid1401.tbin
1402 31 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] MLSCN Grant: 1 X01 MH080680-01 PI Name: Dr. Marvin Gershengorn, NIH NCGC Assay Overview: Confirmation of Thyroid Stimulating Hormone Receptor Agonists TSH is an alpha/beta heterodimeric glycoprotein hormone secreted from the anterior pituitary gland which belongs to the glycoprotein hormone family. The actions of TSH are mediated by a seven-transmembrane receptor, which upon TSH binding couples prefer... aid1402.table aid1402.tbin
1403 29 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] MLSCN Grant: 1 X01 MH080680-01 PI Name: Dr. Marvin Gershengorn, NIH NCGC Assay Overview: Confirmation of Thyroid Stimulating Hormone Receptor Agonists TSH is an alpha/beta heterodimeric glycoprotein hormone secreted from the anterior pituitary gland which belongs to the glycoprotein hormone family. The actions of TSH are mediated by a seven-transmembrane receptor, which upon TSH binding couples prefer... aid1403.table aid1403.tbin
1404 107 Source (MLPCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute (TSRI) Assay Provider: David Frank, Dana Farber Cancer Institute Network: Molecular Libraries Probe Production Centers Network (MLPCN) Grant Proposal Number: 1 X01 MH079826-01 Grant Proposal PI: David Frank, Dana Farber Cancer Institute External Assay ID: STAT3_ACT_LUMI_1536_EC50 (CSDRUN) Name: Dose response counterscreen for STAT1 activators: cell-base... aid1404.table aid1404.tbin
1405 104 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] MLSCN Grant: X01 MH080684-01 PI Name: Dr. Ryszard Kole NCGC Assay Overview: Modulation of Alternative Splicing as Therapy Abnormal splicing is associated with a number of diseases, including cancer and genetic diseases such as cystic fibrosis, muscular dystrophy and beta-thalassemia. Alteration of disease associated splicing patterns provides a promising target for treatment. Work in the Kole laborato... aid1405.table aid1405.tbin
1406 122 Source (MLPCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute (TSRI) Assay Provider: David Frank, Dana Farber Cancer Institute Network: Molecular Libraries Probe Production Centers Network (MLPCN) Grant Proposal Number: 1 X01 MH079826-01 Grant Proposal PI: David Frank, Dana Farber Cancer Institute External Assay ID: STAT1_ACT_LUMI_1536_EC50 (CSDRUN) Name: Dose response counterscreen assay for STAT3 activators: cell-ba... aid1406.table aid1406.tbin
1407 1 Compound effects on the potassium voltage-gated channel KQT-like protein 2 (KCNQ2, Kv7.2) were measured by an optimized rubidium efflux assay. The HEK 293 cells stably expressing KCNQ2 channels were plated into 96-well plates. The next day, cells were loaded with medium containing RbCl. Compounds were added to the cell culture medium after the Rb+ loading. Cells were incubated with 10 uM compound for 3 hours. Then the residual Rb+ was washed out with Rb+ free plain medium. Cells were depolarized ... aid1407.table aid1407.tbin
1408 30 The PKD HTS assay was developed and run at the University of Pittsburgh Molecular Screening Center (PMLSC) as part of the Molecular Library Screening Center Network (MLSCN)(1R03DA24898-01). Protein kinase D (PKD) is a novel family of serine/threonine kinases targeted by diacylglycerol. It regulates many fundamental cell functions including cell proliferation, survival, differentiation and protein trafficking, and plays important roles in pathological conditions such as cardiac hypertrophy and c... aid1408.table aid1408.tbin
1409 53 Source (MLPCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute (TSRI) Assay Provider: David Frank, Dana Farber Cancer Institute Network: Molecular Libraries Probe Production Centers Network (MLPCN) Grant Proposal Number: 1 X01 MH079826-01 Grant Proposal PI: David Frank, Dana Farber Cancer Institute External Assay ID: STAT3_INH_LUMI_1536_IC50 (CSDRUN) Name: Dose response counterscreen for STAT1 inhibitors: cell-base... aid1409.table aid1409.tbin
1410 1090 Source (MLPCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute (TSRI) Assay Provider: Nagi Ayad, TSRI Network: Molecular Libraries Probe Production Centers Network (MLPCN) Grant Proposal Number: 1R21NS056991-01 Grant Proposal PI: Nagi Ayad, TSRI External Assay ID: Wee1_INH_LUMI_1536_3X%ACT Name: Confirmation cell-based high throughput screening assay for inhibitors of Wee1 degradation Description: Cell cycle progression... aid1410.table aid1410.tbin
1411 118 Source (MLPCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute (TSRI) Assay Provider: David Frank, Dana-Farber Cancer Institute Network: Molecular Libraries Probe Production Centers Network (MLPCN) Grant Proposal Number: 1 X01 MH079826-01 Grant Proposal PI: David Frank, Dana-Farber Cancer Institute External Assay ID: STAT1_INH_LUMI_1536_IC50 (CSDRUN) Name: Dose response counterscreen assay for STAT3 inhibitors: cell-ba... aid1411.table aid1411.tbin
1412 38 Source (MLPCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute (TSRI) Assay Provider: Nagi Ayad, TSRI Network: Molecular Libraries Probe Production Centers Network (MLPCN) Grant Proposal Number: 1R21NS056991-01 Grant Proposal PI: Nagi Ayad, TSRI External Assay ID: Wee1Degradation_ACT_LUMI_1536_EC50 Name: Dose Response Cell-based Assay for Inhibitors of Wee1 Degradation Description: Cell cycle progression and entry into... aid1412.table aid1412.tbin
1413 38 Source (MLPCN Center Name): The Scripps Research Institute Molecular Screening Center (SRIMSC) Center Affiliation: The Scripps Research Institute (TSRI) Assay Provider: Franck Madoux, SRIMSC Network: Molecular Libraries Probe Production Centers Network (MLPCN) Grant Proposal Number: 1R21NS056991-01 Grant Proposal PI: Nagi Ayad, TSRI External Assay ID: CytoxHeLa_INH_LUMI_1536_CC50 Name: Cytotoxicity counterscreen assay for inhibitors of Wee1 degradation Description: Cell cycle progression and ... aid1413.table aid1413.tbin
1414 38 Source (MLPCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute (TSRI) Assay Provider: Nagi Ayad, TSRI Network: Molecular Libraries Probe Production Centers Network (MLPCN) Grant Proposal Number: 1R21NS056991-01 Grant Proposal PI: Nagi Ayad, TSRI External Assay ID: cyclinBDegradation_ACT_LUMI_1536_EC50 Name: Counterscreen assay for inhibitors of Wee1 degradation: dose response cell-based assay to identify inhibitors of cy... aid1414.table aid1414.tbin
1415 218702 University of New Mexico Assay Overview: Assay Support: NIH R21NS057014 HTS to identify small molecule regulators of RGS family protein interactions PI: Richard Neubig, Ph.D. Assay Implementation: Yang Wu Ph.D., Mark Haynes Ph.D., Anna Waller Ph.D., Mark Carter MS Target Team Leader for the Center: Larry Sklar, Ph.D., (lsklar@salud.unm.edu) Assay Background and Significance: Regulators of G protein signaling (RGS) proteins are a diverse set of intracellular proteins that modulate G protein-c... aid1415.table aid1415.tbin
1416 218117 Source (MLPCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute (TSRI) Assay Provider: David Ron, New York University Network: Molecular Libraries Probe Production Centers Network (MLPCN) Grant Proposal Number R03 MH082370-01 Grant Proposal PI: David Ron, New York University External Assay ID: PERK_INH_SEAP_1536_%ACT Name: Primary cell-based high-throughput screening assay to measure PERK inhibition Description: The endo... aid1416.table aid1416.tbin
1417 1114 NIH Molecular Libraries Screening Centers Network [MLSCN] Emory Chemical Biology Discovery Center in MLSCN Assay provider: Nikolovska-Coleska, University of Michigan MLSCN Grant: R21NS057014 The Bcl-2 protein family includes anti-apoptotic proteins such as Bcl-2, Bcl-xL and Mcl-1 and pro-apoptotic proteins such as Bak, Bax, Bim, Bid and Bad. All members of the Bcl-2 protein family contain at least one conserved Bcl-2 homology (BH) domain. These domains have been demonstrated to be involved in th... aid1417.table aid1417.tbin
1418 1176 NIH Molecular Libraries Screening Centers Network [MLSCN] Emory Chemical Biology Discovery Center in MLSCN Assay provider: Nikolovska-Coleska, University of Michigan MLSCN Grant: R21NS057014 The Bcl-2 protein family includes anti-apoptotic proteins such as Bcl-2, Bcl-xL and Mcl-1 and pro-apoptotic proteins such as Bak, Bax, Bim, Bid and Bad. All members of the Bcl-2 protein family contain at least one conserved Bcl-2 homology (BH) domain. These domains have been demonstrated to be involved in th... aid1418.table aid1418.tbin
1419 849 NIH Molecular Libraries Screening Centers Network [MLSCN] Emory Chemical Biology Discovery Center in MLSCN Assay provider: Theodore Jardetzky; Northwestern University MLSCN Grant: 1R21NS059415-01 Epstein-Barr virus (EBV), or human herpes virus 4 (HHV-4), is a member of the larger herpesvirus family that consists of three subfamilies (##, ##, ##). Epstein-Barr virus (EBV) is an extremely prevalent human herpesvirus. Disease syndromes in humans caused by EBV reflect the cell types that EBV infects... aid1419.table aid1419.tbin
1420 1134 NIH Molecular Libraries Screening Centers Network [MLSCN] Emory Chemical Biology Discovery Center in MLSCN Assay provider: Jonathan Glass, MD, Emory University School of Medicine MLSCN Grant: R03DA024890-01 Calpains are ubiquitous, calcium-activated cysteine proteases involved in both physiological and pathological cellular functions. The two major forms, u-calpain (calpain I) and m-calpain (calpain II), are activated by micromolar and millimolar calcium concentrations, respectively. A current... aid1420.table aid1420.tbin
1421 1344 NIH Molecular Libraries Screening Centers Network [MLSCN] Emory Chemical Biology Discovery Center in MLSCN Assay provider: Dr. Raymond Dingledine, Emory University MLSCN Grant: 5-U01NS058158-02 Assay Overview: Injury of the brain is a major cause of death and morbidity after the prolonged seizures termed status epilepticus (SE). Studies in rodents have demonstrated that cyclooxygenase 2 (COX2) activation by ischemia and SE generally contributes to neuronal injury, but multiple downstream COX2 si... aid1421.table aid1421.tbin
1422 256758 NIH Molecular Libraries Screening Centers Network [MLSCN] Emory Chemical Biology Discovery Center in MLSCN Assay provider: Dr. Raymond Dingledine, Emory University MLSCN Grant: 5-U01NS058158-02 Assay Overview: Prostaglandin E2 that is produced by COX2 in response to cellular injury is involved in a multimodal inflammatory response in many tissues, including the brain. Studies in rodents have demonstrated that cyclooxygenase 2 (COX2) activation following ischemia and status epilepticus generall... aid1422.table aid1422.tbin
1423 218702 University of New Mexico Assay Overview: Assay Support: NIH R21NS057014 HTS to identify small molecule regulators of RGS family protein interactions PI: Richard Neubig, Ph.D. Assay Implementation: Yang Wu Ph.D., Mark Haynes Ph.D., Anna Waller Ph.D., Mark Carter MS Target Team Leader for the Center: Larry Sklar, Ph.D., (lsklar@salud.unm.edu) Assay Background and Significance: Regulators of G protein signaling (RGS) proteins are a diverse set of intracellular proteins that modulate G protein-c... aid1423.table aid1423.tbin
1424 218117 Source (MLPCN Center Name): The Scripps Research Institute Molecular Screening Center (SRIMSC) Center Affiliation: The Scripps Research Institute (TSRI) Assay Provider: Stefan Heller, Stanford University Network: Molecular Libraries Probe Production Centers Network (MLPCN) Grant Proposal Number 1 R03 MH083077-01 Grant Proposal PI: Stefan Heller, Stanford University External Assay ID: TRPN1_AG_Calcium_1536_%ACT Name: Primary cell-based high-throughput screening assay to identify agonists of the... aid1424.table aid1424.tbin
1425 60 This assay details affinity data for agonists at human 5-Hydroxytryptamine 5-HT1A receptor, as described in the published literature. Where possible the data described are at transfected receptors expressed in cell lines and the ligands described are those that are potent, selective, endogenous, or those used as prescription medications. aid1425.table aid1425.tbin
1426 1 This assay details affinity data for allosteric regulators at human 5-Hydroxytryptamine 5-HT1A receptor, as described in the published literature. Where possible the data described are at transfected receptors expressed in cell lines and the ligands described are those that are potent, selective, endogenous, or those used as prescription medications. aid1426.table aid1426.tbin
1427 2 This assay details affinity data for antagonists at rat 5-Hydroxytryptamine 5-HT1A receptor, as described in the published literature. Where possible the data described are at transfected receptors expressed in cell lines and the ligands described are those that are potent, selective, endogenous, or those used as prescription medications. aid1427.table aid1427.tbin
1428 43 This assay details affinity data for antagonists at human 5-Hydroxytryptamine 5-HT1A receptor, as described in the published literature. Where possible the data described are at transfected receptors expressed in cell lines and the ligands described are those that are potent, selective, endogenous, or those used as prescription medications. aid1428.table aid1428.tbin
1429 237 Source (MLPCN Center Name): The Scripps Research Institute Molecular Screening Center (SRIMSC) Center Affiliation: The Scripps Research Institute (TSRI) Assay Provider: Hugh Rosen, TSRI Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: 1 R03 MH076533-01 Grant Proposal PI: Germana Sanna, TSRI External Assay ID: S1P3_ANT_BLA_1536_3X%INH Name: Confirmation cell-based assay to identify antagonists of the Sphingosine 1-Phosphate Receptor 3 (S1P3) Description: Sp... aid1429.table aid1429.tbin
1430 220335 Excerpt from MH0882340 application (Dr. James Morris, Clemson University) Trypanosoma brucei, the digenic protozoan parasite that causes African sleeping sickness in man, annually infects ~500,000 people in sub-Saharan Africa, leading to 50,000-70,000 deaths per year. Glucose metabolism is essential for the parasite, with the pathogenic lifestage of the parasite, the bloodstream form (BSF), acquiring energy exclusively through glycolysis. Hexokinase (HK), the first enzyme in glycolysis, cata... aid1430.table aid1430.tbin
1431 1260 Molecular Library Screening Center Network (MLSCN) Penn Center for Molecular Discovery (PCMD) Assay Provider: Scott L. Diamond, University of Pennsylvania MLSCN Grant: X01-MH076406-01 Target Human kallikrein 5 (hK5) is a member of the human tissue kallikrein family, which contains 15 kallikrein-like serine proteases (1). It is synthesized as a 293 amino acid zymogen, and loses a 29 amino acid signal peptide upon secretion, followed by cleavage at the Arg66-Ile67 bond, which releases a 37 ami... aid1431.table aid1431.tbin
1433 38 Kinase inhibitors are a new class of therapeutics with a propensity to inhibit multiple targets. The biological consequences of multi-kinase activity are poorly defined, and an important step toward understanding the relationship between selectivity, efficacy and safety is the exploration of how inhibitors interact with the human kinome. We present interaction maps for 38 kinase inhibitors across a panel of 317 kinases representing >50% of the predicted human protein kinome. The data constitute t... aid1433.table aid1433.tbin
1434 218386 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Production Centers Network (MLPCN) Grant Number: X01 MH083230-01 Assay Provider: Dr. John Bushweller, University of Virginia Charlottesville, Charlottesville VA The protein-protein interaction between the subunits of the heterodimeric transcription factor CBF, core binding factor beta (CBFb) and Runx1 (CBFa), pla... aid1434.table aid1434.tbin
1435 68 Project Title: A screen for modulators of human Rad51, a key DNA repair protein Application Number: MH084119 Assay Submitter: Dr. Alex Mazin Submitter Institution: Drexel University Screening Center Name: Penn Center for Molecular Discovery (PCMD) Principal Investigator of Screening Center: Scott Diamond Ionizing radiation (IR) and inter-strand cross-linking agents (ICL) induce DNA double-stranded breaks (DSB). DSB are the most harmful type of DNA damage, which cause genome instability, can... aid1435.table aid1435.tbin
1436 19 Project Title: A screen for modulators of human Rad51, a key DNA repair protein Application Number: MH084119 Assay Submitter: Dr. Alex Mazin Submitter Institution: Drexel University Screening Center Name: Penn Center for Molecular Discovery (PCMD) Principal Investigator of Screening Center: Scott Diamond Ionizing radiation (IR) and inter-strand cross-linking agents (ICL) induce DNA double-stranded breaks (DSB). DSB are the most harmful type of DNA damage, which cause genome instability, can... aid1436.table aid1436.tbin
1437 95 Project Title: A screen for modulators of human Rad51, a key DNA repair protein Application Number: MH084119 Assay Submitter: Dr. Alex Mazin Submitter Institution: Drexel University Screening Center Name: Penn Center for Molecular Discovery (PCMD) Principal Investigator of Screening Center: Scott Diamond Ionizing radiation (IR) and inter-strand cross-linking agents (ICL) induce DNA double-stranded breaks (DSB). DSB are the most harmful type of DNA damage, which cause genome instability, can... aid1437.table aid1437.tbin
1438 2224 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Production Centers Network (MLPCN) Grant Number: X01 MH083230-01 Assay Provider: Dr. John Bushweller, University of Virginia Charlottesville, Charlottesville VA The protein-protein interaction between the subunits of the heterodimeric transcription factor CBF, core binding factor b (CBFb) and Runx1 (CBFa), plays ... aid1438.table aid1438.tbin
1439 218702 University of New Mexico Assay Overview: Assay Support: NIH R21NS057014 HTS to identify small molecule regulators of RGS family protein interactions PI: Richard Neubig, Ph.D. Assay Implementation: Yang Wu Ph.D., Mark Haynes Ph.D., Anna Waller Ph.D., Mark Carter MS Target Team Leader for the Center: Larry Sklar, Ph.D., (lsklar@salud.unm.edu) Assay Background and Significance: Regulators of G protein signaling (RGS) proteins are a diverse set of intracellular proteins that modulate G protein-c... aid1439.table aid1439.tbin
1440 218702 University of New Mexico Assay Overview: Assay Support: NIH R21NS057014 HTS to identify small molecule regulators of RGS family protein interactions PI: Richard Neubig, Ph.D. Assay Implementation: Yang Wu Ph.D., Mark Haynes Ph.D., Anna Waller Ph.D., Mark Carter MS Target Team Leader for the Center: Larry Sklar, Ph.D., (lsklar@salud.unm.edu) Assay Background and Significance: Regulators of G protein signaling (RGS) proteins are a diverse set of intracellular proteins that modulate G protein-c... aid1440.table aid1440.tbin
1441 218702 University of New Mexico Assay Overview: Assay Support: NIH R21NS057014 HTS to identify small molecule regulators of RGS family protein interactions PI: Richard Neubig, Ph.D. Assay Implementation: Yang Wu Ph.D., Mark Haynes Ph.D., Anna Waller Ph.D., Mark Carter MS Target Team Leader for the Center: Larry Sklar, Ph.D., (lsklar@salud.unm.edu) Assay Background and Significance: Regulators of G protein signaling (RGS) proteins are a diverse set of intracellular proteins that modulate G protein-c... aid1441.table aid1441.tbin
1442 40 Molecular Library Screening Center Network (MLSCN) Penn Center for Molecular Discovery (PCMD) Assay Provider: Brent Stockwell, Columbia University MLSCN Grant: R03MH082369-01 The E3 ligases are involved in regulating other proteins by covalent ligation to the 76 amino acid protein ubiquitin. This post-translational modification can result in altered conformation, altered activity, or degradation of the substrate protein. Thus, E3 ligases are effectors of a major means of post-translational modif... aid1442.table aid1442.tbin
1443 217187 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Production Centers Network (MLPCN) Grant Number: X01 MH083230-01 Assay Provider: Dr. Dmitri Rozanov, Sanford-Burnham Medical Research Institute, San Diego CA Cytotoxic chemotherapy induces apoptosis via a pathway involving mitochondria, sometimes referred to as the "intrinsic pathway." An acquired resistance to a... aid1443.table aid1443.tbin
1444 40 Molecular Library Screening Center Network (MLSCN) Penn Center for Molecular Discovery (PCMD) Assay Provider: Brent Stockwell, Columbia University MLSCN Grant: R03MH082369-01 The E3 ligases are involved in regulating other proteins by covalent ligation to the 76 amino acid protein ubiquitin. This post-translational modification can result in altered conformation, altered activity, or degradation of the substrate protein. Thus, E3 ligases are effectors of a major means of post-translational modif... aid1444.table aid1444.tbin
1445 217157 Southern Research Molecular Libraries Screening Center (SRMLSC) Southern Research Institute (Birmingham, Alabama) NIH Molecular Libraries Screening Centers Network (MLSCN) Assay Provider: Dr. Donald Gardiner, Queensland Institute of Medical Research Grant: 1-R03-MH082342-01A1 The intraerythrocytic stages of the human malaria parasite Plasmodium falciparum employs two cytosolic neutral aminopeptidases, an M1-family alanyl aminopeptidase (M1AAP) and an M17-family leucine aminopeptidase (M17LAP... aid1445.table aid1445.tbin
1446 218117 Source (MLPCN Center Name): The Scripps Research Institute Molecular Screening Center (SRIMSC) Center Affiliation: The Scripps Research Institute (TSRI) Assay Provider: Ross Levine, Memorial Sloan Kettering Cancer Center (MSKCC) Network: Molecular Libraries Probe Production Centers Network (MLPCN) Grant Proposal Number: 1 R03 MH084174-01 Grant Proposal PI: Ross Levine, MSKCC External Assay ID: JAK2V617F_INH_LUMI_1536_%INH Name: Primary cell-based high throughput assay for inhibitors of the Janus... aid1446.table aid1446.tbin
1447 1248 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Production Centers Network (MLPCN) Grant Number: X01 MH083230-01 Assay Provider: Dr. Dmitri Rosenoff, Sanford-Burnham Medical Research Institute, San Diego CA This assay is a counter screen for AID 1443, "uHTS for the identification of compounds that potentiate TRAIL-induced apoptosis of cancer cells". The goa... aid1447.table aid1447.tbin
1448 218117 Source (MLPCN Center Name): The Scripps Research Institute Molecular Screening Center (SRIMSC) Center Affiliation: The Scripps Research Institute (TSRI) Assay Provider: Stefan Heller, Stanford University Network: Molecular Libraries Probe Production Centers Network (MLPCN) Grant Proposal Number 1 R03 MH083077-01 Grant Proposal PI: Stefan Heller , Stanford University External Assay ID: TRPML3_AG_Calcium_1536_%ACT Name: Primary cell-based high-throughput screening assay to identify agonists of the... aid1448.table aid1448.tbin
1449 6 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Proposal Number: MH081277-01 Assay Provider: John C. Reed, Sanford-Burnham Medical Research Institute, San Diego, CA This XIAP dose response assay is developed and performed to confirm hits originally identified in the XIAP HTS binding assay (AID 1018) and to study the struc... aid1449.table aid1449.tbin
1450 39 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Proposal Number: 1R03 MH082385-01 This TNAP dose response assay is developed and performed to confirm hits originally identified in the TNAP luminescent HTS assay (AID 1001) and to study the structure-activity relationship on analogs of the confirmed hits. Compounds are eith... aid1450.table aid1450.tbin
1451 273 University of New Mexico Assay Overview: Assay Support: 1R03MH081228-01A1 High-throughput multiplex screening for ABC transporter inhibitors PI: Richard Larson MD, PhD Assay Development: Irena Ivnitski-Steele PhD Assay Implementation: Irena Ivnitski-Steele PhD, Terry Foutz, Anna Waller PhD, Mark Carter Target Team Leader for the Center: Bruce Edwards, PhD (BEdwards@salud.unm.edu) Assay Background and Significance: The three major types of multidrug resistance (MDR) proteins in humans incl... aid1451.table aid1451.tbin
1452 153607 Assay Provider: Holman, T.R., University of California, Santa Cruz Screening Center PI: Austin, C.P. Screening Center: NIH Chemical Genomics Center [NCGC] Human lipoxygenase 12hLO is a member of the closely related lipoxygenase family of enzymes which catalyze the site-specific oxidation of arachidonic acid to various hormone precursor molecules and as such is a candidate for drug development in a variety of disease areas, such as cancer and inflammation. Inhibition of 12hLO activity was screene... aid1452.table aid1452.tbin
1453 273 University of New Mexico Assay Overview: Assay Support:1R03MH081228-01A1 High-throughput multiplex screening for ABC transporter inhibitors PI: Richard Larson MD, PhD Assay Development: Irena Ivnitski-Steele PhD Assay Implementation: Irena Ivnitski-Steele PhD, Terry Foutz, Anna Waller PhD, Mark Carter Target Team Leader for the Center: Bruce Edwards, PhD (BEdwards@salud.unm.edu Assay Background and Significance: The three major types of multidrug resistance (MDR) proteins in humans include... aid1453.table aid1453.tbin
1454 133385 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Screening Centers Network [MLSCN] MLSCN Grant: 1X01MH082406-01 Assay Submitter (PI): Dr. Wei Zheng, NCGC/NIH NCGC Assay Overview: The Ras/extracellular-signal-regulated kinase (ERK) mitogen activated protein (MAP) kinase signaling pathway (ERK1/2 cascade) plays a key role in transmitting signals from the cell surface to the nucleus (Nishida and Gotoh 1993; Chang and Karin, 2001). The cascade is initiated by the small G-protein Ras, w... aid1454.table aid1454.tbin
1455 29 NIH Molecular Libraries Screening Centers Network [MLSCN] NIH Chemical Genomics Center [NCGC] MLSCN Grant: XO1-MH079852-01 PI Name: Nicholson, Ben. Progenra Inc, Malvern, PA NCGC Assay Overview: Homeostasis of cellular proteins is maintained through a combination of synthesis and degradation. The pathway that accounts for the majority of protein degradation is the ubiquitin-proteasomal pathway. Ubiquitin (Ub) is highly conserved in all cells and the generation of a multi-Ub chain typically tar... aid1455.table aid1455.tbin
1456 189275 Vanderbilt Screening Center for GPCRs, Ion Channels and Transporters Assay Provider: Eric Delpire Assay Provider Affliation: Vanderbilt University Grant Title: Identification of Novel Modulators of Cl- dependent Transport Process via HTS Grant Number: R21NS053658-01 Cation-chloride cotransporters such as K-Cl cotransport and Na-K-2Cl cotransport play major roles in a variety of physiological settings, including the modulation of GABAergic synaptic transmission. For instance, KCC2, a neuronal-spe... aid1456.table aid1456.tbin
1457 208882 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Probe Production centers Network [MLPCN] MLPCN Grant: X01 MH082413-01 Assay Submitter (PI): Wei Zheng NCGC Assay Overview: Lithium has been widely used for the treatment of bipolar disorder. But lithium has a narrow therapeutic index and it can cause side effects such as thirst, weight gain, tremor, polyuria and memory problems. Although the mechanism for lithium action in treatment of bipolar disorder is still not fully understood, i... aid1457.table aid1457.tbin
1458 211511 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Probe Production centers Network [MLPCN] MLPCN Grant: R03 MH084179-01 Assay Submitter (PI): Elliot Androphy NCGC Assay Overview: Spinal muscular atrophy (SMA) is caused by insufficient levels of the survival motor neuron protein SMN. The SMN locus on chromosome 5q13 contains two inverted copies of SMN called SMN1 and SMN2 which are 99% identical at the amino acid level. SMN1 is a fully functional protein and SMN2 skips exon 7 90% of t... aid1458.table aid1458.tbin
1459 1279 NCGC Assay Overview: Hutchinson-Gilford Progeria Syndrome (HGPS) is a pediatric premature aging disease caused by a spontaneous mutation in the lamin A/C (LMNA) gene. The mutation activates a cryptic splice site in the LMNA pre-mRNA which results in production of a pre-lamin A protein that cannot be processed properly. The mutant protein accumulates in the nucleus and negatively affects numerous cellular functions. Correction of the splicing defect in HGPS patient cells using a targeted oligonu... aid1459.table aid1459.tbin
1460 271676 NIH Molecular Libraries Probe Production Network [MLPCN] NIH Chemical Genomics Center [NCGC] MLPCN Grant: X01 MH083262-01 Assay Provider: Carlo Ballatore, University of Pennsylvania NCGC Assay Overview: The microtubule-associated protein tau is an abundant protein in the axons of neurons that stabilizes microtubules. With its ability to modulate microtubule dynamics, tau contributes directly or indirectly, to key structural and regulatory cellular functions. Particularly important is the influ... aid1460.table aid1460.tbin
1461 221370 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Probe Production centers Network [MLPCN] MLPCN Grant: X01-DA026210-01 Assay Submitter (PI): Heilig, Markus Alexander NCGC Assay Overview: Neuropeptide S receptor (NPSR), previously known as GPR154, is a recently de-orphanized G protein coupled receptor. Its endogenous ligand is the 20 amino acids peptide Neuropeptide S (NPS). Activation of NPSR induces transient increases in intracellular calcium and cAMP, suggesting coupling of this ... aid1461.table aid1461.tbin
1462 6 NIH Molecular Libraries Screening Centers Network [MLSCN] Emory Chemical Biology Discovery Center in MLSCN MLSCN Grant Number: MH077612-01 Escherichia coli DnaK, a homolog of heat shock protein 70, has been shown to protect denature proteins from aggregation and promote their refolding by ATP hydrolysis. DnaK, along with its two co-cohort proteins DnaJ and GrpE, forms a microbial chaperone system that shelters microorganisms from environmental stresses such as temperature, osmotic, and pH change... aid1462.table aid1462.tbin
1463 275712 NIH Molecular Libraries Probe Production Network [MLPCN] NIH Chemical Genomics Center [NCGC] MLPCN Grant: X01 MH083262-01 Assay Provider: Carlo Ballatore, University of Pennsylvania NCGC Assay Overview: The microtubule-associated protein tau is an abundant protein in the axons of neurons that stabilizes microtubules. With its ability to modulate microtubule dynamics, tau contributes directly or indirectly, to key structural and regulatory cellular functions. Particularly important is the influ... aid1463.table aid1463.tbin
1464 11 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Probe Production centers Network [MLPCN] MLPCN Grant: X01-DA026210-01 Assay Submitter (PI): Heilig, Markus Alexander NCGC Assay Overview: Neuropeptide S receptor (NPSR), previously known as GPR154, is a recently de-orphanized G protein coupled receptor. Its endogenous ligand is the 20 amino acids peptide Neuropeptide S (NPS). Activation of NPSR induces transient increases in intracellular calcium and cAMP, suggesting coupling of this ... aid1464.table aid1464.tbin
1465 215402 Screen for Probes that give insight into the mitochondrial electron transport chain Friedreich's ataxia is an autosomal recessive neurodegenerative disorder caused by mutations in the FXN gene, which encodes the protein frataxin [Campuzano, et al., 1996, Ohshima, et al. 1998], and for which there are no currently accepted treatments. It is the most common hereditary ataxia and causes progressive damage to the nervous system, particularly sensory neurons, resulting in symptoms ranging from atax... aid1465.table aid1465.tbin
1466 199303 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Probe Production centers Network [MLPCN] MLPCN Grant: 1R03MH084841-01 Assay Submitter (PI): Wei Zheng NCGC Assay Overview: Alpha-glucosidase is responsible for hydrolysis of terminal, non-reducing 1,4-linked alpha-D-glucose residues with release of alpha-D-glucose. It is a lysosomal hydrolase that is required for the degradation of a small percentage (1-3%) of cellular glycogen in human. Deficiency of this enzyme results in glycogen-s... aid1466.table aid1466.tbin
1467 229459 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Probe Production centers Network [MLPCN] MLPCN Grant: 1R03MH084842-01 Assay Submitter (PI): Wei Zheng NCGC Assay Overview: Alpha-galactosidase is a homodimeric glycoprotein that hydrolyzes the terminal alpha-galactosyl moieties from glycolipids and glycoproteins. Deficiency of this enzyme results in Fabry Disease with progressive accumulation of globotriaosylceramide and other glycosphingolipids in vascular endothelial cells that caus... aid1467.table aid1467.tbin
1468 275712 NIH Molecular Libraries Probe Production Network [MLPCN] NIH Chemical Genomics Center [NCGC] MLPCN Grant: X01 MH083262-01 Assay Provider: Carlo Ballatore, University of Pennsylvania NCGC Assay Overview: The microtubule-associated protein tau is an abundant protein in the axons of neurons that stabilizes microtubules. With its ability to modulate microtubule dynamics, tau contributes directly or indirectly, to key structural and regulatory cellular functions. Particularly important is the influ... aid1468.table aid1468.tbin
1469 282587 NIH Molecular Libraries Probe Production Network [MLPCN] NIH Chemical Genomics Center [NCGC] MLPCN Grant: DK058080 Assay Provider: R. Kip Guy, St. Jude Children's Research Hospital NCGC Assay Overview: Thyroid receptor (TR) regulates many homeostatic processes including basal metabolism, cardiovascular function, body weight, and lipid trafficking. TR modulators are potential therapeutics for obesity and hyperlipidemias but current thyroid analogs have undesirable side effects, particularly card... aid1469.table aid1469.tbin
1470 4 Assay Provider: P. Jeffrey Conn Assay Provider Affiliation: Vanderbilt University Grant Title: Discovery of novel allosteric modulators of the M1 muscarinic receptor Grant Number: 1 R03 MH077606-01 The M1 muscarinic receptor is thought to be an important therapeutic target in schizophrenia. A cell-based fluorometric calcium assay was developed for high throughput screening. This assay was used to identify compounds with high selectivity for the M1 receptor subtype that act at an allosteric site ... aid1470.table aid1470.tbin
1471 227407 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Probe Production centers Network [MLPCN] MLPCN Grant: R03MH084839-01A1 Assay Submitter (PI): Wei Zheng NCGC Assay Overview: Huntington's disease is a neurodegenerative disorder caused by a trinucleotide repeat expansion in Exon 1 of the Huntingtin gene. The CAG trinucleotide encodes glutamine and polyglutamine expansions cause cell death in selective areas of the brain. Huntington polyglutamine repeats have the tendency to form aggreg... aid1471.table aid1471.tbin
1472 15 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Probe Production centers Network [MLPCN] MLPCN Grant: 1R03MH084842-01 Assay Submitter (PI): Wei Zheng NCGC Assay Overview: Alpha-galactosidase is a homodimeric glycoprotein that hydrolyzes the terminal alpha-galactosyl moieties from glycolipids and glycoproteins. Deficiency of this enzyme results in Fabry Disease with progressive accumulation of globotriaosylceramide and other glycosphingolipids in vascular endothelial cells that caus... aid1472.table aid1472.tbin
1473 5 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Probe Production centers Network [MLPCN] MLPCN Grant: 1R03MH084841-01 Assay Submitter (PI): Wei Zheng NCGC Assay Overview: Alpha-glucosidase is responsible for hydrolysis of terminal, non-reducing 1,4-linked alpha-D-glucose residues with release of alpha-D-glucose. It is a lysosomal hydrolase that is required for the degradation of a small percentage (1-3%) of cellular glycogen in human. Deficiency of this enzyme results in glycogen-s... aid1473.table aid1473.tbin
1474 38 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Probe Production centers Network [MLPCN] MLPCN Grant: R03 MH084179-01 Assay Submitter (PI): Elliot Androphy NCGC Assay Overview: Spinal muscular atrophy (SMA) is caused by insufficient levels of the survival motor neuron protein SMN. The SMN locus on chromosome 5q13 contains two inverted copies of SMN called SMN1 and SMN2 which are 99% identical at the amino acid level. SMN1 is a fully functional protein and SMN2 skips exon 7 90% of t... aid1474.table aid1474.tbin
1475 21 NIH Molecular Libraries Probe Production Network [MLPCN] NIH Chemical Genomics Center [NCGC] MLPCN Grant: X01 MH083262-01 Assay Provider: Carlo Ballatore, University of Pennsylvania NCGC Assay Overview: The microtubule-associated protein tau is an abundant protein in the axons of neurons that stabilizes microtubules. With its ability to modulate microtubule dynamics, tau contributes directly or indirectly, to key structural and regulatory cellular functions. Particularly important is the infl... aid1475.table aid1475.tbin
1476 197846 Cruzain is a cysteine protease from the tropical parasite Trypanosoma cruzi. Dual qHTS experiment was performed against the NCGC compound collection in order to 1) identify novel inhibitors of the enzyme and 2) profile the compound collection for promiscuous inhibitors operating via colloidal aggregation (by performing detergent-free and detergent-containing comparison screens). Cruzain was assayed by the use of fluorogenic coumarin-based substrate Z-FR-AMC: proteolytic cleavage releases AMC, who... aid1476.table aid1476.tbin
1477 234798 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Probe Production centers Network [MLPCN] MLPCN Grant: X01MH083259-01 Assay Submitter (PI): Paul Liu NCGC Assay Overview: Core Binding Factor (CBF) abnormalities are associated with 20-25% of all acute myeloid leukemias (AML), of which 5-10% are further sub classified as acute myelomonocytic leukemia with eosinophilia, also known as M4Eo in the FAB classification scheme. This subtype of leukemia is usually associated with chromosome ... aid1477.table aid1477.tbin
1478 197846 Cruzain (3.4.22.51) is a cysteine protease from the tropical parasite Trypanosoma cruzi. Dual qHTS experiment was performed against the NCGC compound collection in order to 1) identify novel inhibitors of the enzyme and 2) profile the compound collection for promiscuous inhibitors operating via colloidal aggregation (by performing detergent-free and detergent-containing comparison screens). Cruzain was assayed by the use of fluorogenic coumarin-based substrate Z-FR-AMC: proteolytic cleavage relea... aid1478.table aid1478.tbin
1479 282587 NIH Molecular Libraries Probe Production Network [MLPCN] NIH Chemical Genomics Center [NCGC] MLPCN Grant: DK058080 Assay Provider: R. Kip Guy, St. Jude Children's Research Hospital NCGC Assay Overview: Thyroid receptor (TR) regulates many homeostatic processes including basal metabolism, cardiovascular function, body weight, and lipid trafficking. TR modulators are potential therapeutics for obesity and hyperlipidemias but current thyroid analogs have undesirable side effects, particularly car... aid1479.table aid1479.tbin
1480 273 University of New Mexico Assay Overview: Assay Support: 1R03MH081228-01A1 High-throughput multiplex screening for ABC transporter inhibitors PI: Richard Larson MD, PhD Assay Development: Irena Ivnitski-Steele PhD Assay Implementation: Irena Ivnitski-Steele PhD, Terry Foutz, Anna Waller PhD, Mark Carter Target Team Leader for the Center: Bruce Edwards, PhD (BEdwards@salud.unm.edu) Assay Background and Significance: For the multiplex screen of ABC transporters (AID 1325 and 1326) testing co... aid1480.table aid1480.tbin
1481 218117 Source (MLPCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute (TSRI) Assay Provider: Raymond Deshaies, California Institute of Technology Network: Molecular Libraries Probe Production Centers Network (MLPCN) Grant Proposal Number 1 R03 MH085687-01 Grant Proposal PI: Raymond Deshaies, California Institute of Technology External Assay ID: P97_INH_Lumi_1536_%INH Name: Primary biochemical high-throughput screening assay to me... aid1481.table aid1481.tbin
1482 11 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Probe Production centers Network [MLPCN] MLPCN Grant: R03MH084839-01A1 Assay Submitter (PI): Wei Zheng NCGC Assay Overview: Huntington's disease is a neurodegenerative disorder caused by a trinucleotide repeat expansion in Exon 1 of the Huntingtin gene. The CAG trinucleotide encodes glutamine and polyglutamine expansions cause cell death in selective areas of the brain. Huntington polyglutamine repeats have the tendency to form aggreg... aid1482.table aid1482.tbin
1483 273 University of New Mexico Assay Overview: Assay Support: 1R03MH081228-01A1 High-throughput multiplex screening for ABC transporter inhibitors PI: Richard Larson MD, PhD Assay Development: Irena Ivnitski-Steele PhD Assay Implementation: Irena Ivnitski-Steele PhD, Terry Foutz, Anna Waller PhD, Mark Carter Target Team Leader for the Center: Bruce Edwards, PhD (BEdwards@salud.unm.edu) Assay Background and Significance: For the multiplex screen of ABC transporters (AID 1325 and 1326) testing co... aid1483.table aid1483.tbin
1484 15 NCGC Assay Overview: Core Binding Factor (CBF) abnormalities are associated with 20-25% of all acute myeloid leukemias (AML), of which 5-10% are further sub classified as acute myelomonocytic leukemia with eosinophilia, also known as M4Eo in the FAB classification scheme. This subtype of leukemia is usually associated with chromosome 16 inversion (p13:q22). Chromosome 16 inversion results in formation of the fusion oncogene CBFB-MYH11, which encodes the fusion protein CBF-beta-SMMHC. This fusion... aid1484.table aid1484.tbin
1485 1 NIH Molecular Libraries Probe Production Network [MLPCN] NIH Chemical Genomics Center [NCGC] MLPCN Grant: DK058080 Assay Provider: R. Kip Guy, St. Jude Children's Research Hospital NCGC Assay Overview: Thyroid receptor (TR) regulates many homeostatic processes including basal metabolism, cardiovascular function, body weight, and lipid trafficking. TR modulators are potential therapeutics for obesity and hyperlipidemias but current thyroid analogs have undesirable side effects, particularly car... aid1485.table aid1485.tbin
1486 218117 Source (MLPCN Center Name): The Scripps Research Institute Molecular Screening Center (SRIMSC) Center Affiliation: The Scripps Research Institute (TSRI) Assay Provider: Ross Levine, Memorial Sloan Kettering Cancer Center (MSKCC) Network: Molecular Libraries Probe Production Centers Network (MLPCN) Grant Proposal Number: 1 R03 MH084174-01 Grant Proposal PI: Ross Levine, MSKCC External Assay ID: BAF3CYTOX_INH_LUMI_1536_%INH Name: Counterscreen for inhibitors of Janus kinase 2 mutant JAK2V617F: Cel... aid1486.table aid1486.tbin
1487 198098 NCGC Assay Overview: Hutchinson-Gilford Progeria Syndrome (HGPS) is a pediatric premature aging disease caused by a spontaneous mutation in the lamin A/C (LMNA) gene. The mutation activates a cryptic splice site in the LMNA pre-mRNA which results in production of a pre-lamin A protein that cannot be processed properly. The mutant protein accumulates in the nucleus and negatively affects numerous cellular functions. Correction of the splicing defect in HGPS patient cells using a targeted oligonuc... aid1487.table aid1487.tbin
1488 1665 Assay Provider: P. Jeffery Conn Assay Provider Affiliation: Vanderbilt University Grant Title: Discovery of novel allosteric modulators of the M1 muscarinic receptor Grant Number: 1 R03 MH077606-01 The M1 muscarinic receptor is thought to be an important therapeutic target in schizophrenia. A cell-based fluorometric calcium assay was developed for high throughput screening. This assay was used to identify compounds with high selectivity for the M1 receptor subtype that act at an allosteric site ... aid1488.table aid1488.tbin
1489 213 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Probe Production centers Network [MLPCN] MLPCN Grant: X01-DA026210-01 Assay Submitter (PI): Heilig, Markus Alexander NCGC Assay Overview: Neuropeptide S receptor (NPSR), previously known as GPR154, is a recently de-orphanized G protein coupled receptor. Its endogenous ligand is the 20 amino acids peptide Neuropeptide S (NPS). Activation of NPSR induces transient increases in intracellular calcium and cAMP, suggesting coupling of this ... aid1489.table aid1489.tbin
1490 310852 Assay Submitter: Michael Burkart, University of California, San Diego Screening Center PI: Austin, C.P. Screening Center: NIH Chemical Genomics Center [NCGC] The covalent attachment of a phosphopantetheinyl (4'-PP) arm to a variety of synthases and other proteins is a key posttranslational protein modification. The 4'-PP is installed on the proteins post-translationally from coenzyme A (CoA) on a conserved serine residue by action of phosphopantetheinyl transferase (PPTase) enzymes. Phosphopante... aid1490.table aid1490.tbin
1491 259 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Probe Production centers Network [MLPCN] MLPCN Grant: X01-DA026210-01 Assay Submitter (PI): Heilig, Markus Alexander NCGC Assay Overview: Neuropeptide S receptor (NPSR), previously known as GPR154, is a recently de-orphanized G protein coupled receptor. Its endogenous ligand is the 20 amino acids peptide Neuropeptide S (NPS). Activation of NPSR induces transient increases in intracellular calcium and cAMP, suggesting coupling of this ... aid1491.table aid1491.tbin
1492 85 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Probe Production centers Network [MLPCN] MLPCN Grant: X01-DA026210-01 Assay Submitter (PI): Heilig, Markus Alexander NCGC Assay Overview: Neuropeptide S receptor (NPSR), previously known as GPR154, is a recently de-orphanized G protein coupled receptor. Its endogenous ligand is the 20 amino acids peptide Neuropeptide S (NPS). Activation of NPSR induces transient increases in intracellular calcium and cAMP, suggesting coupling of this ... aid1492.table aid1492.tbin
1493 16 NIH Chemical Genomics Center [NCGC] NIH Molecular Libraries Probe Production centers Network [MLPCN] MLPCN Grant: X01-DA026210-01 Assay Submitter (PI): Heilig, Markus Alexander NCGC Assay Overview: Neuropeptide S receptor (NPSR), previously known as GPR154, is a recently de-orphanized G protein coupled receptor. Its endogenous ligand is the 20 amino acids peptide Neuropeptide S (NPS). Activation of NPSR induces transient increases in intracellular calcium and cAMP, suggesting coupling of this ... aid1493.table aid1493.tbin
1494 11 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Proposal Number: XO1 MH078942 Assay Provider: Dr. Maurizio Pellecchia, Sanford-Burnham Medical Research Institute, San Diego, CA The misregulation of protein folding often results in a variety of deleterious consequences on cellular function that range from the accumulation ... aid1494.table aid1494.tbin
1495 16 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Proposal Number: XO1 MH078942 Assay Provider: Dr. Maurizio Pellecchia, Sanford-Burnham Medical Research Institute, San Diego, CA The misregulation of protein folding often results in a variety of deleterious consequences on cellular function that range from the accumulation ... aid1495.table aid1495.tbin
1496 215818 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Production Centers Network (MLPCN) Grant Number: X01 MH083230-01 Assay Provider: Dr. John Bushweller, University of Virginia Charlottesville, Charlottesville VA The protein-protein interaction between the subunits of the heterodimeric transcription factor CBF, core binding factor b (CBFb) and Runx1 (CBFa), plays ... aid1496.table aid1496.tbin
1497 92523 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Production Centers Network (MLPCN) Grant Number: 1R03NS053659-01 Assay Provider: Dr. Vineet Gupta, University of Miami, Miami FL The leukocyte specific beta2 integrins are central to the biological function of these cells. These cellular receptors mediate the divalent metal ion dependent adhesion of leukocytes in... aid1497.table aid1497.tbin
1498 152 NCGC Assay Overview: Hutchinson-Gilford Progeria Syndrome (HGPS) is a pediatric premature aging disease caused by a spontaneous mutation in the lamin A/C (LMNA) gene. The mutation activates a cryptic splice site in the LMNA pre-mRNA which results in production of a pre-lamin A protein that cannot be processed properly. The mutant protein accumulates in the nucleus and negatively affects numerous cellular functions. Correction of the splicing defect in HGPS patient cells using a targeted oligonuc... aid1498.table aid1498.tbin
1499 92690 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Production Centers Network (MLPCN) Grant Number: 1R03NS053659-01 Assay Provider: Dr. Vineet Gupta, University of Miami, Miami FL The leukocyte specific beta2 integrins are central to the biological function of these cells. These cellular receptors mediate the divalent metal ion dependent adhesion of leukocytes inc... aid1499.table aid1499.tbin
1500 4 Principal Investigator: Hattie D. Gresham, Ph.D. Grant: NIH 1X01MH078952-01 Screening Center: New Mexico Molecular Libraries Screening Center Assay Background and Significance Quorum sensing is a cell-to-cell communication system that permits members of a bacterial population to coordinate their behavior dependent on cell density (for review see Waters and Bassler, 2005). The mediators of this communication system are small, diffusible pheromones or autoinducers that are secreted by the bacte... aid1500.table aid1500.tbin
1501 1 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Proposal Number: XO1 MH078942 Assay Provider: Dr. Maurizio Pellecchia, Sanford-Burnham Medical Research Institute, San Diego, CA The misregulation of protein folding often results in a variety of deleterious consequences on cellular function that range from the accumulation ... aid1501.table aid1501.tbin
1502 475 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Production Centers Network (MLPCN) Grant Number: X01 MH083230-01 Assay Provider: Dr. John Bushweller, University of Virginia Charlottesville, Charlottesville VA The protein-protein interaction between the subunits of the heterodimeric transcription factor CBF, core binding factor beta (CBFb) and Runx1 (CBFa), pla... aid1502.table aid1502.tbin
1503 8 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Proposal Number: XO1 MH078942 Assay Provider: Dr. Maurizio Pellecchia, Sanford-Burnham Medical Research Institute, San Diego, CA The misregulation of protein folding often results in a variety of deleterious consequences on cellular function that range from the accumulation ... aid1503.table aid1503.tbin
1504 1629 University of New Mexico Assay Overview: Assay Support: NIH R21NS057014 HTS to identify small molecule regulators of RGS family protein interactions PI: Larry Sklar, Ph.D., Richard Neubig, Ph.D Assay Implementatiion: Yang Wu Ph.D, Mark Hynes Ph.D, Anna Waller Ph.D, Mark Carter MS Assay Background and Significance: This report summarizes the series of assays used to identify small molecule modulators of regulators of G protein signaling (RGS) family protein interactions via the G protein a... aid1504.table aid1504.tbin
1505 2 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Proposal Number: XO1 MH078942 Assay Provider: Dr. Maurizio Pellecchia, Sanford-Burnham Medical Research Institute, San Diego, CA The misregulation of protein folding often results in a variety of deleterious consequences on cellular function that range from the accumulation ... aid1505.table aid1505.tbin
1506 4 Principal Investigator: Hattie D. Gresham, Ph.D. Grant: NIH 1X01MH078952-01 Screening Center: New Mexico Molecular Libraries Screening Center Assay Background and Significance Quorum sensing is a cell-to-cell communication system that permits members of a bacterial population to coordinate their behavior dependent on cell density (for review see Waters and Bassler, 2005). The mediators of this communication system are small, diffusible pheromones or autoinducers that are secreted by the bacte... aid1506.table aid1506.tbin
1507 4 Principal Investigator: Hattie D. Gresham, Ph.D. Grant: NIH 1X01MH078952-01 Screening Center: New Mexico Molecular Libraries Screening Center Assay Background and Significance Quorum sensing is a cell-to-cell communication system that permits members of a bacterial population to coordinate their behavior dependent on cell density (for review see Waters and Bassler, 2005). The mediators of this communication system are small, diffusible pheromones or autoinducers that are secreted by the bacte... aid1507.table aid1507.tbin
1508 4 Assay Provider: P. Jeffrey Conn Assay Provider Affiliation: Vanderbilt University Grant Title: Discovery of novel allosteric modulators of the M1 muscarinic receptor Grant Number: 1 R03 MH077606-01 The M1 muscarinic receptor is thought to be an important therapeutic target in schizophrenia. A cell-based fluorometric calcium assay was developed for high throughput screening. This assay was used to identify compounds with high selectivity for the M1 receptor subtype that act at an allosteric site ... aid1508.table aid1508.tbin
1509 218117 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute (TSRI) Assay Provider: Michael Oldstone, TSRI Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: U01 AI074564 Grant Proposal PI: Michael Oldstone, TSRI External Assay ID: S1P4_AG_BLA_1536_%ACT Name: Primary Cell-Based Assay to Identify Agonists of the Sphingosine 1-Phosphate Receptor 4 (S1P4) Description: Pandemic influenza repr... aid1509.table aid1509.tbin
1510 218117 Source (MLSCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute (TSRI) Assay Provider: Michael Oldstone, TSRI Network: Molecular Library Screening Center Network (MLSCN) Grant Proposal Number: U01 AI074564 Grant Proposal PI: Michael Oldstone, TSRI External Assay ID: S1P4_ANT_BLA_1536_%INH Name: Primary Cell-Based Assay to Identify Antagonists of the Sphingosine 1-Phosphate Receptor 4 (S1P4) Description: Pandemic influenza... aid1510.table aid1510.tbin
1511 305679 Source (MLPCN Center Name): Johns Hopkins Ion Channel Center (JHICC) Center Affiliation: Johns Hopkins University, School of Medicine Assay Provider: Dr. Sabina Kupershmidt, Vanderbilt University Medical Center Network: Molecular Libraries Probe Production Centers Network (MLPCN) Grant Proposal Number: 1R03MH084820-01 Grant Proposal PI: Dr. Sabina Kupershmidt, Vanderbilt University Medical Center Assay Implementation: Beiyan Zou Ph.D., Shunyou Long M.S., Amy Scott M.S., Haibo Yu Ph.D., Meng Wu Ph... aid1511.table aid1511.tbin
1512 274 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Proposal Number: MH077602-01 Assay Provider: Dr. Jose Luis Millan, Sanford-Burnham Medical Research Institute, San Diego, CA. This PLAP dose response assay is developed and performed to confirm hits originally identified in the PLAP Luminescent HTS assay (AID 690) and to stu... aid1512.table aid1512.tbin
1513 238 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Proposal Number: MH081277-01 Assay Provider: John C. Reed, Sanford-Burnham Medical Research Institute, San Diego, CA This XIAP-Bir3 dose response assay is developed and performed as a counter screen for hits originally identified in the XIAP-Bir1/2 HTS binding assay (AID 101... aid1513.table aid1513.tbin
1514 4 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Proposal Number: MH081277-01 Assay Provider: John C. Reed, Sanford-Burnham Medical Research Institute, San Diego, CA This dose response assay is developed and performed as a counter screen to compounds in the Chemical Antagonists of IAP-family anti-apoptotic proteins confirm... aid1514.table aid1514.tbin
1515 218117 Source (MLPCN Center Name): The Scripps Research Institute Molecular Screening Center (SRIMSC) Center Affiliation: The Scripps Research Institute (TSRI) Assay Provider: Benjamin Cravatt, TSRI Network: Molecular Libraries Probe Production Centers Network (MLPCN) Grant Proposal Number: 1 R01 CA087660-05 Fast Track Grant Proposal PI: Benjamin Cravatt, TSRI External Assay ID: RBBP9_INH_FP_1536_%INH Name: Primary biochemical high throughput screening assay to identify inhibitors of Retinoblastoma bin... aid1515.table aid1515.tbin
1516 31 Source (MLPCN Center Name): The Scripps Research Institute Molecular Screening Center (SRIMSC) Center Affiliation: The Scripps Research Institute (TSRI) Assay Provider: Hugh Rosen, TSRI Network: Molecular Library Probe Production Centers Network (MLPCN) Grant Proposal Number: 1 R03 MH076533-01 Grant Proposal PI: Germana Sanna, TSRI External Assay ID: 5HT1E_ANT_BLA_384_IC50 CS Name: Counterscreen assay for S1P3 antagonists: Dose response cell-based high throughput screening assay to identify anta... aid1516.table aid1516.tbin
1517 759 Source (MLPCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute (TSRI) Assay Provider: Raymond Deshaies, California Institute of Technology Network: Molecular Libraries Probe Production Centers Network (MLPCN) Grant Proposal Number: 1 R03 MH085687-01 Grant Proposal PI: Raymond Deshaies, California Institute of Technology External Assay ID: p97_INH_Lumi_1536_3X%INH Name: Confirmation biochemical high-throughput screening ass... aid1517.table aid1517.tbin
1518 31 Source (MLPCN Center Name): The Scripps Research Institute Molecular Screening Center (SRIMSC) Center Affiliation: The Scripps Research Institute (TSRI) Assay Provider: Hugh Rosen, TSRI Network: Molecular Library Probe Production Centers Network (MLPCN) Grant Proposal Number: 1 R03 MH076533-01 Grant Proposal PI: Germana Sanna, TSRI External Assay ID: S1P3_ANT_BLA_384_IC50 Name: Dose response cell-based high throughput screening assay for antagonists of the Sphingosine 1-Phosphate Receptor 3 (S1P... aid1518.table aid1518.tbin
1519 8868 NIH Molecular Libraries Probe Production Centers Network [MLPCN] NIH Chemical Genomics Center [NCGC] MLPCN Grant: TBA Assay Submitter (PI): Beller, Mathias; Max-Planck-Institut fur Biophysikalische Chemie NCGC Assay Overview: Storing lipids as a reservoir for energy or the anabolism of elementary metabolites is a common feature of probably all cells and is conserved from bacteria to humans. The universal cellular lipid storage organelle is the so-called lipid storage droplet (LD). Although ubiq... aid1519.table aid1519.tbin
1520 576 Source (MLPCN Center Name): The Scripps Research Institute Molecular Screening Center (SRIMSC) Center Affiliation: The Scripps Research Institute (TSRI) Assay Provider: Ross Levine, Memorial Sloan Kettering Cancer Center (MSKCC) Network: Molecular Libraries Probe Production Centers Network (MLPCN) Grant Proposal Number: 1 R03 MH084174-01 Grant Proposal PI: Ross Levine, MSKCC External Assay ID: BAF3CYTOX_INH_LUMI_1536_3X%INH Name: Counterscreen for inhibitors of mutant JAK2V617F: Cell-based high t... aid1520.table aid1520.tbin
1521 576 Source (MLPCN Center Name): The Scripps Research Institute Molecular Screening Center (SRIMSC) Center Affiliation: The Scripps Research Institute (TSRI) Assay Provider: Ross Levine, Memorial Sloan Kettering Cancer Center (MSKCC) Network: Molecular Libraries Probe Production Centers Network (MLPCN) Grant Proposal Number: 1 R03 MH084174-01 Grant Proposal PI: Ross Levine, MSKCC External Assay ID: JAK2V617F_INH_LUMI_1536_3X%INH Name: Confirmation cell-based high throughput screening assay for inhibi... aid1521.table aid1521.tbin
1522 118 Source (MLPCN Center Name): The Scripps Research Institute Molecular Screening Center (SRIMSC) Center Affiliation: The Scripps Research Institute (TSRI) Assay Provider: David Ron, New York University Network: Molecular Libraries Probe Production Centers Network (MLPCN) Grant Proposal Number R03 MH082370-01 Grant Proposal PI: David Ron, New York University External Assay ID: PERK_INH_SEAP_1536_EC50 Name: Dose response cell-based high-throughput screening assay to measure PERK inhibition Descripti... aid1522.table aid1522.tbin
1523 737 Source (MLPCN Center Name): The Scripps Research Institute Molecular Screening Center (SRISMC) Center Affiliation: The Scripps Research Institute (TSRI) Assay Provider: Michael Oldstone, TSRI Network: Molecular Library Probe Production Centers Network (MLPCN) Grant Proposal Number: U01 AI074564 Fast Track Grant Proposal PI: Michael Oldstone, TSRI External Assay ID: S1P4_AG_BLA_1536_3X%ACT Name: Confirmation cell-based high throughput assay for agonists of the Sphingosine 1-Phosphate Receptor 4 (... aid1523.table aid1523.tbin
1524 515 Source (MLPCN Center Name): The Scripps Research Institute Molecular Screening Center (SRISMC) Center Affiliation: The Scripps Research Institute (TSRI) Assay Provider: Michael Oldstone, TSRI Network: Molecular Library Probe Production Centers Network (MLPCN) Grant Proposal Number: U01 AI074564 Fast Track Grant Proposal PI: Michael Oldstone, TSRI External Assay ID: S1P4_ANT_BLA_1536_3X%INH Name: Confirmation cell-based high throughput assay for antagonists of the Sphingosine 1-Phosphate Receptor... aid1524.table aid1524.tbin
1525 306 Source (MLPCN Center Name): The Scripps Research Institute Molecular Screening Center (SRIMSC) Center Affiliation: The Scripps Research Institute (TSRI) Assay Provider: Stefan Heller, Stanford University Network: Molecular Libraries Probe Production Centers Network (MLPCN) Grant Proposal Number 1 R03 MH083077-01 Grant Proposal PI: Stefan Heller , Stanford University External Assay ID: TRPN1_AG_Calcium_1536_3X%ACT CSRUN Name: Counterscreen assay for TRPML3 agonists: cell-based high-throughput scre... aid1525.table aid1525.tbin
1526 306 Source (MLPCN Center Name): The Scripps Research Institute Molecular Screening Center (SRIMSC) Center Affiliation: The Scripps Research Institute (TSRI) Assay Provider: Stefan Heller, Stanford University Network: Molecular Libraries Probe Production Centers Network (MLPCN) Grant Proposal Number 1 R03 MH083077-01 Grant Proposal PI: Stefan Heller , Stanford University External Assay ID: TRPML3_AG_Calcium_1536_3X%ACT Name: Confirmation cell-based high-throughput screening assay for agonists of the ... aid1526.table aid1526.tbin
1527 290893 Source (MLPCN Center Name): The Scripps Research Institute Molecular Screening Center (SRIMSC) Center Affiliation: The Scripps Research Institute (TSRI) Assay Provider: Peter Hodder, TSRI Network: Molecular Libraries Probe Production Centers Network (MLPCN) Grant Proposal Number: 1 R21 NS059451-01 Fast Track Grant Proposal PI: Peter Hodder, TSRI External Assay ID: VIM-2_INH_EPIABS_1536_%INH Name: Primary biochemical high throughput screening assay to identify inhibitors of VIM-2 metallo-beta-la... aid1527.table aid1527.tbin
1528 118 Source (MLPCN Center Name): The Scripps Research Institute Molecular Screening Center (SRIMSC) Center Affiliation: The Scripps Research Institute (TSRI) Assay Provider: David Ron, New York University Network: Molecular Libraries Probe Production Centers Network (MLPCN) Grant Proposal Number R03 MH082370-01 Grant Proposal PI: David Ron, New York University External Assay ID: PERK_INH_SEAP_1536_EC50 6HR CS Name: Counterscreen assay for PERK inhibitors: Dose response cell-based high throughput scre... aid1528.table aid1528.tbin
1529 289974 University of New Mexico Assay Overview: Assay Support: 1R03MH084830-01 Project Title: TR-FRET HTS Assay for Inhibitors of MEKK2-MEK5 PB1 Domain Interaction PI: Kazuhiro Nakamura, Ph.D Center PI: Larry Sklar, Ph.D Assay Implementatiion: Zurab Surviladze Ph.D, Mark Haynes Ph.D, Anna Waller Ph.D, Mark Carter MS Assay Background and Significance: PB1 (Phox/Bem1p) domains function as protein-protein interaction sites by forming PB1-PB1 domain heterodimers (Moscat, et al. 2006). There are at... aid1529.table aid1529.tbin
1530 289939 University of New Mexico Assay Overview: Assay Support: 1R03MH084830-01 Project Title: TR-FRET HTS Assay for Inhibitors of MEKK2-MEK5 PB1 Domain Interaction PI: Kazuhiro Nakamura, Ph.D Center PI: Larry Sklar, Ph.D Assay Implementatiion: Zurab Surviladze Ph.D, Mark Haynes Ph.D, Anna Waller Ph.D, Mark Carter MS Assay Background and Significance: PB1 (Phox/Bem1p) domains function as protein-protein interaction sites by forming PB1-PB1 domain heterodimers (Moscat, et al. 2006). There are at... aid1530.table aid1530.tbin
1531 289866 University of New Mexico Assay Overview: Assay Support: 1R03MH084830-01 Project Title: TR-FRET HTS Assay for Inhibitors of MEKK2-MEK5 PB1 Domain Interaction PI: Kazuhiro Nakamura, Ph.D Center PI: Larry Sklar, Ph.D Assay Implementatiion: Zurab Surviladze Ph.D, Mark Haynes Ph.D, Anna Waller Ph.D, Mark Carter MS Assay Background and Significance: PB1 (Phox/Bem1p) domains function as protein-protein interaction sites by forming PB1-PB1 domain heterodimers (Moscat, et al. 2006). There are at... aid1531.table aid1531.tbin
1532 106289 Molecular Library Screening Center Network (MLSCN) Penn Center for Molecular Discovery (PCMD) Assay Provider: Michael McNeil, Colorado State University, Fort Collins, CO MLSCN Grant: DA024889-01 This screen is for compounds that have the potential to be developed into new drugs against tuberculosis (TB) because they inhibit the enzymes required for the formation of the cell wall of the tuberculosis bacterium. New drugs are needed because the rate of cure with the present drugs is very slow, and ... aid1532.table aid1532.tbin
1533 158970 Molecular Library Screening Center Network (MLSCN) Penn Center for Molecular Discovery (PCMD) Assay Provider: Michael McNeil, Colorado State University, Fort Collins, CO MLSCN Grant: DA024889-01 This screen is for compounds that have the potential to be developed into new drugs against tuberculosis (TB) because they inhibit the enzymes required for the formation of the cell wall of the tuberculosis bacterium. New drugs are needed because the rate of cure with the present drugs is very slow, and ... aid1533.table aid1533.tbin
1534 54 Source (MLPCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute (TSRI) Assay Provider: Raymond Deshaies, California Institute of Technology Network: Molecular Libraries Probe Production Centers Network (MLPCN) Grant Proposal Number: 1 R03 MH085687-01 Grant Proposal PI: Raymond Deshaies, California Institute of Technology External Assay ID: p97_INH_Lumi_384_IC50 Name: Dose response biochemical high throughput screening assay... aid1534.table aid1534.tbin
1535 202 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Number: R03 MH082386-01 Assay Provider: Dr. Hudson H. Freeze, Sanford-Burnham Medical Research Institute, San Diego, CA Congenital Disorders of Glycosylation (CDG) are autosomal recessive defects in the synthesis of N-linked oligosaccharide chains. CDG group I (CDG-I) defect... aid1535.table aid1535.tbin
1536 16 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Number: R03 MH082386-01 Assay Provider: Dr. Hudson H. Freeze, Sanford-Burnham Medical Research Institute, San Diego, CA Congenital Disorders of Glycosylation (CDG) are autosomal recessive defects in the synthesis of N-linked oligosaccharide chains. CDG group I (CDG-I) defect... aid1536.table aid1536.tbin
1537 408 Source (MLPCN Center Name): The Scripps Research Institute Molecular Screening Center (SRIMSC) Center Affiliation: The Scripps Research Institute (TSRI) Assay Provider: Benjamin Cravatt, TSRI Network: Molecular Libraries Probe Production Centers Network (MLPCN) Grant Proposal Number: 1 R01 CA087660-05 Fast Track Grant Proposal PI: Benjamin Cravatt, TSRI External Assay ID: RBBP9_INH_FP_1536_3X%INH Name: Confirmation biochemical high throughput screening assay for inhibitors of Retinoblastoma bind... aid1537.table aid1537.tbin
1538 77 Source (MLPCN Center Name): The Scripps Research Institute Molecular Screening Center (SRIMSC) Center Affiliation: The Scripps Research Institute (TSRI) Assay Provider: Stefan Heller, Stanford University Network: Molecular Libraries Probe Production Centers Network (MLPCN) Grant Proposal Number 1 R03 MH083077-01 Grant Proposal PI: Stefan Heller , Stanford University External Assay ID: TRPN1_AG_Calcium_1536_EC50 Name: Dose response cell-based high-throughput screening assay for agonists of the tr... aid1538.table aid1538.tbin
1539 453 Source (MLPCN Center Name): The Scripps Research Institute Molecular Screening Center (SRIMSC) Center Affiliation: The Scripps Research Institute (TSRI) Assay Provider: Patricia McDonald, Scripps Florida Network: Molecular Library Probe Production Centers Network (MLPCN) Grant Proposal Number 1 R21 NS056950-01 Grant Proposal PI: Claes Wahlestedt External Assay ID: NPY-Y2_POT_CNGC_1536_3X%ACT Name: Confirmation cell-based high-throughput screening assay for potentiators or agonists of NPY-Y2 Desc... aid1539.table aid1539.tbin
1540 165 Confirmatory assay NIH Chemical Genomics Center [NCGC] Matthew G. Vander Heiden, M.D., Ph.D. [Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115] NCGC Assay Overview: Human pyruvate kinase muscle 2 (hPK-M2)enzyme was supplied as a highly purified (>95% pure) preparation from Harvard Medical School and a secondary assay was used to evaluate compounds. This assay couples the formation of pyruvate from hPK-M2 using lactate dehydrogenase (LDH) and NADH. The depletion of NADH is foll... aid1540.table aid1540.tbin
1541 120 Confirmatory assay NIH Chemical Genomics Center [NCGC] Matthew G. Vander Heiden, M.D., Ph.D. [Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115] NCGC Assay Overview: Human pyruvate kinase liver (hPK-L)enzyme was supplied as a highly purified (>95% pure) preparation from Harvard Medical School and a secondary assay was used to evaluate compounds. This assay couples the formation of pyruvate from hPK-R using lactate dehydrogenase (LDH) and NADH. The depletion of NADH is followed i... aid1541.table aid1541.tbin
1542 131 Confirmatory assay NIH Chemical Genomics Center [NCGC] Matthew G. Vander Heiden, M.D., Ph.D. [Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115] NCGC Assay Overview: Human pyruvate kinase liver (hPK-M1)enzyme was supplied as a highly purified (>95% pure) preparation from Harvard Medical School and a secondary assay was used to evaluate compounds. This assay couples the formation of pyruvate from hPK-R using lactate dehydrogenase (LDH) and NADH. The depletion of NADH is followed ... aid1542.table aid1542.tbin
1543 108 Confirmatory assay NIH Chemical Genomics Center [NCGC] Wael M. Rabeh, Lyudmila Nedyalkova and Hee-Wan Park [Structural Genomics Consortium, 100 College St. Toronto, Ontario, Canada] NCGC Assay Overview: Human pyruvate kinase reticulocyte (hPK-R) enzyme was supplied as a highly purified (>95% pure) preparation from Structural Genomics Consortium in Toronto (Ontario, Canada) and a secondary assay was used to evaluate compounds. This assay couples the formation of pyruvate from hPK-R using lactate... aid1543.table aid1543.tbin
1544 54 Source (MLPCN Center Name): The Scripps Research Institute Molecular Screening Center Center Affiliation: The Scripps Research Institute (TSRI) Assay Provider: Raymond Deshaies, California Institute of Technology Network: Molecular Libraries Probe Production Centers Network (MLPCN) Grant Proposal Number: 1 R03 MH085687-01 Grant Proposal PI: Raymond Deshaies, California Institute of Technology External Assay ID: P97C522A_INH_LUMI_384_IC50 CS Name: Luminescence counterscreen assay for p97 inhibit... aid1544.table aid1544.tbin
1545 2 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Number: R03 MH082386-01 Assay Provider: Dr. Hudson H. Freeze, Sanford-Burnham Medical Research Institute, San Diego, CA Congenital Disorders of Glycosylation (CDG) are autosomal recessive defects in the synthesis of N-linked oligosaccharide chains. CDG group I (CDG-I) defect... aid1545.table aid1545.tbin
1546 281 Source (MLPCN Center Name): The Scripps Research Institute Molecular Screening Center (SRIMSC) Center Affiliation: The Scripps Research Institute (TSRI) Assay Provider: Claes Wahlestedt, Scripps Florida Network: Molecular Library Probe Production Centers Network (MLPCN) Grant Proposal Number 1 R21 NS056950-01 Grant Proposal PI: Claes Wahlestedt External Assay ID: NPY-Y1_POT_CNGC_1536_3X%ACT Name: Confirmation cell-based high-throughput screening assay for potentiators or agonists of NPY-Y1 Descr... aid1546.table aid1546.tbin
1547 1 Data Source: University of Maryland BioAssay Type: Secondary Description: NIH Molecular Libraries Probe Production Centers Network [MLPCN] NIH Chemical Genomics Center [NCGC] Assay Submitter (PI): Carole Sztalryd; GRECC/Geriatrics, Veterans Affairs Medical Center, Department of Medicine, School of Medicine, University of Maryland, Baltimore, Maryland, USA. Beller, Mathias; Max-Planck-Institut fur Biophysikalische Chemie, Germany. NCGC Assay Overview: Storing lipids as a reservoir for energy ... aid1547.table aid1547.tbin
1548 2 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Proposal Number: 1R03 MH082385-01 Assay Providers: Drs. Jose Luis Millan and Eduard Sergienko, Sanford-Burnham Medical Research Institute, San Diego, CA. Alkaline phosphatases (EC 3.1.3.1) (APs) catalyze the hydrolysis of phosphomonoesters, releasing phosphate and alcohol. A... aid1548.table aid1548.tbin
1549 3677 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Number: R03MH082366 Assay Provider: Dr. Maurizio Pellecchia, Sanford-Burnham Medical Research Institute Of the current available drugs against influenza A virus, two target the M2 proton channel [1]. These are the adamantane-based compounds Amantadine and its close analogu... aid1549.table aid1549.tbin
1550 6 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Production Centers Network (MLPCN) Grant Number: X01 MH083230-01 Assay Provider: Dr. John Bushweller, University of Virginia Charlottesville, Charlottesville VA The protein-protein interaction between the subunits of the heterodimeric transcription factor CBF, core binding factor beta(CBFb) and Runx1 (CBFa), play... aid1550.table aid1550.tbin
1551 16 Source (MLPCN Center Name): The Scripps Research Institute Molecular Screening Center (SRIMSC) Center Affiliation: The Scripps Research Institute (TSRI) Assay Provider: Raymond Deshaies, California Institute of Technology Network: Molecular Libraries Probe Production Centers Network (MLPCN) Grant Proposal Number: 1 R03 MH085687-01 Grant Proposal PI: Raymond Deshaies, California Institute of Technology External Assay ID: P97ATP_INH_LUMI_384_IC50_SAR_round1 Name: Luminescence dose response biochem... aid1551.table aid1551.tbin
1552 3677 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Number: XO1 MH078942 Assay Provider: Dr. Maurizio Pellecchia, Sanford-Burnham Medical Research Institute The ubiquitin-proteasome system has won the reputation of the cellular recycling center that degraded aggregated or misfolded proteins in the cell (ref. [1] and referenc... aid1552.table aid1552.tbin
1553 90 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Number: R03 MH082386-01 Assay Provider: Dr. Hudson H. Freeze, Sanford-Burnham Medical Research Institute, San Diego, CA Congenital Disorders of Glycosylation (CDG) are autosomal recessive defects in the synthesis of N-linked oligosaccharide chains. CDG group I (CDG-I) defect... aid1553.table aid1553.tbin
1554 303344 Broad Institute MLPCN Ras Selective Lethality Project Project ID: 2013 Keywords: Ras, apoptosis, cancer, VDAC, oxidative cell death Primary Collaborators: Brent Stockwell, Columbia University, 614 Fairchild Center, MC 2406, 1212 Amesterdam Ave, New York, NY 10027, bs2189@columbia.edu, 212.854.2948 Dan Zaharevitz, NCI Science Officer, zaharevd@mail.nih.gov Project Overview: The goal of the project is to identify small molecules in the MLSMR and related compound synthesized through follow-up... aid1554.table aid1554.tbin
1555 1 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, Lake Nona, FL) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Proposal Number: Assay Provider: Dr. Layton Smith, Sanford-Burnham Medical Research Institute This assay was established to measure the extent of potential hepatic metabolism of compounds. Liver microsomes consist mainly of endoplasmatic reticulum, contain many drug-metabol... aid1555.table aid1555.tbin
1556 290893 Source (MLPCN Center Name): The Scripps Research Institute Molecular Screening Center (SRIMSC) Center Affiliation: The Scripps Research Institute (TSRI) Assay Provider: Peter Hodder, TSRI Network: Molecular Libraries Probe Production Centers Network (MLPCN) Grant Proposal Number: 1 R21 NS059451-01 Fast Track Grant Proposal PI: Peter Hodder, TSRI External Assay ID: IMP-1_INH_EPIABS_1536_%INH Name: Fluorescence primary biochemical high throughput screening assay to identify inhibitors of IMP-1 meta... aid1556.table aid1556.tbin
1557 1 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, Lake Nona, FL) Network: NIH Molecular Libraries Probe Production Centers Network (MLPCN) Grant Proposal Number: Assay Provider: Dr. Layton Smith, Sanford-Burnham Medical Research Institute Evaluating compound permeability through cell a monolayer is a good indication of intestinal permeability and oral bioavailability. The parallel artificial membrane permeabi... aid1557.table aid1557.tbin
1558 138 NIH Molecular Libraries Probe Production Network [MLPCN] NIH Chemical Genomics Center [NCGC] MLPCN Grant: X01 MH083262-01 Assay Provider: Carlo Ballatore, University of Pennsylvania NCGC Assay Overview: The microtubule-associated protein tau is an abundant protein in the axons of neurons that stabilizes microtubules. With its ability to modulate microtubule dynamics, tau contributes directly or indirectly, to key structural and regulatory cellular functions. Particularly important is the influ... aid1558.table aid1558.tbin
1559 138 NIH Molecular Libraries Probe Production Network [MLPCN] NIH Chemical Genomics Center [NCGC] MLPCN Grant: X01 MH083262-01 Assay Provider: Carlo Ballatore, University of Pennsylvania NCGC Assay Overview: The microtubule-associated protein tau is an abundant protein in the axons of neurons that stabilizes microtubules. With its ability to modulate microtubule dynamics, tau contributes directly or indirectly, to key structural and regulatory cellular functions. Particularly important is the influ... aid1559.table aid1559.tbin
1560 13 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Production Centers Network (MLPCN) Grant Number: X01 MH083230-01 Assay Provider: Dr. John Bushweller, University of Virginia Charlottesville, Charlottesville VA The protein-protein interaction between the subunits of the heterodimeric transcription factor CBF, core binding factor beta (CBFb) and Runx1 (CBFa), pla... aid1560.table aid1560.tbin
1561 1 NIH Molecular Libraries Probe Production Centers Network [MLPCN] NIH Chemical Genomics Center [NCGC] MLPCN Grant: TBA Assay Submitter (PI): Beller, Mathias; Max-Planck-Institut fur Biophysikalische Chemie, Germany. NCGC Assay Overview: Storing lipids as a reservoir for energy or the anabolism of elementary metabolites is a common feature of probably all cells and is conserved from bacteria to humans. The universal cellular lipid storage organelle is the so-called lipid storage droplet (LD). Alt... aid1561.table aid1561.tbin
1562 188 Source (MLPCN Center Name): The Scripps Research Institute Molecular Screening Center (SRIMSC) Center Affiliation: The Scripps Research Institute (TSRI) Assay Provider: Stefan Heller, Stanford University Network: Molecular Libraries Probe Production Centers Network (MLPCN) Grant Proposal Number 1 R03 MH083077-01 Grant Proposal PI: Stefan Heller , Stanford University External Assay ID: TRPML3_AG_Calcium_1536_EC50 Name: Dose response cell-based high-throughput screening assay for agonists of the tr... aid1562.table aid1562.tbin
1563 737 Source (MLPCN Center Name): The Scripps Research Institute Molecular Screening Center (SRIMSC) Center Affiliation: The Scripps Research Institute (TSRI) Assay Provider: Michael Oldstone, TSRI Network: Molecular Library Probe Production Center Network (MLPCN) Grant Proposal Number: U01 AI074564 Fast Track Grant Proposal PI: Michael Oldstone, TSRI External Assay ID: S1P1_AG_BLA_1536_3X%ACT Counterscreen Name: Counterscreen assay for S1P4 agonists: Cell-based high throughput screening assay to iden... aid1563.table aid1563.tbin
1564 515 Source (MLPCN Center Name): The Scripps Research Institute Molecular Screening Center (SRIMSC) Center Affiliation: The Scripps Research Institute (TSRI) Assay Provider: Michael Oldstone, TSRI Network: Molecular Library Probe Production Center Network (MLPCN) Grant Proposal Number: U01 AI074564 Fast Track Grant Proposal PI: Michael Oldstone, TSRI External Assay ID: S1P1_ANT_BLA_1536_3X%ACT Counterscreen Name: Counterscreen assay for S1P4 antagonists: Cell-based high throughput screening assay to... aid1564.table aid1564.tbin
1565 288481 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Production Centers Network (MLPCN) Grant Number: 1 R03 MH084086-01 Assay Provider: Dr. Jose Luis Milan, Sanford-Burnham Medical Research Institute, San Diego CA Mineralization of cartilage and bone occurs by a series of physicochemical and biochemical processes that together facilitate the deposition of hydroxyap... aid1565.table aid1565.tbin
1566 292486 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Probe Production Centers Network (MLPCN) Grant Number: 1 R03 MH084844-01 Assay Provider: Dr. John C. Reed, Sanford-Burnham Medical Research Institute, San Diego CA The modulation of immune response activity is one of the major goals in the development of novel therapeutics for auto-immune and inflammatory diseases... aid1566.table aid1566.tbin
1567 103 NIH Molecular Libraries Probe Production Network [MLPCN] NIH Chemical Genomics Center [NCGC] MLPCN Grant: DK058080 Assay Provider: R. Kip Guy, St. Jude Children's Research Hospital NCGC Assay Overview: Thyroid receptor (TR) regulates many homeostatic processes including basal metabolism, cardiovascular function, body weight, and lipid trafficking. TR modulators are potential therapeutics for obesity and hyperlipidemias but current thyroid analogs have undesirable side effects, particularly car... aid1567.table aid1567.tbin
1568 103 NIH Molecular Libraries Probe Production Network [MLPCN] NIH Chemical Genomics Center [NCGC] MLPCN Grant: DK058080 Assay Provider: R. Kip Guy, St. Jude Children's Research Hospital NCGC Assay Overview: Thyroid receptor (TR) regulates many homeostatic processes including basal metabolism, cardiovascular function, body weight, and lipid trafficking. TR modulators are potential therapeutics for obesity and hyperlipidemias but current thyroid analogs have undesirable side effects, particularly car... aid1568.table aid1568.tbin
1569 749 NIH Molecular Libraries Probe Production Centers Network [MLPCN] NIH Chemical Genomics Center [NCGC] MLPCN Grant: 1 R03 MH085686-01 Assay Submitter (PI): Beller, Mathias; Max-Planck-Institut fur Biophysikalische Chemie NCGC Assay Overview: Storing lipids as a reservoir for energy or the anabolism of elementary metabolites is a common feature of probably all cells and is conserved from bacteria to humans. The universal cellular lipid storage organelle is the so-called lipid storage droplet (LD).... aid1569.table aid1569.tbin
1570 112 NIH Molecular Libraries Probe Production Network [MLPCN] NIH Chemical Genomics Center [NCGC] MLPCN Grant: DK058080 Assay Provider: R. Kip Guy, St. Jude Children's Research Hospital NCGC Assay Overview: Thyroid receptor (TR) regulates many homeostatic processes including basal metabolism, cardiovascular function, body weight, and lipid trafficking. TR modulators are potential therapeutics for obesity and hyperlipidemias but current thyroid analogs have undesirable side effects, particularly card... aid1570.table aid1570.tbin
1571 112 NIH Molecular Libraries Probe Production Network [MLPCN] NIH Chemical Genomics Center [NCGC] MLPCN Grant: DK058080 Assay Provider: R. Kip Guy, St. Jude Children's Research Hospital NCGC Assay Overview: Thyroid receptor (TR) regulates many homeostatic processes including basal metabolism, cardiovascular function, body weight, and lipid trafficking. TR modulators are potential therapeutics for obesity and hyperlipidemias but current thyroid analogs have undesirable side effects, particularly card... aid1571.table aid1571.tbin
1572 112 NIH Molecular Libraries Probe Production Network [MLPCN] NIH Chemical Genomics Center [NCGC] MLPCN Grant: DK058080 Assay Provider: R. Kip Guy, St. Jude Children's Research Hospital NCGC Assay Overview: Thyroid receptor (TR) regulates many homeostatic processes including basal metabolism, cardiovascular function, body weight, and lipid trafficking. TR modulators are potential therapeutics for obesity and hyperlipidemias but current thyroid analogs have undesirable side effects, particularly card... aid1572.table aid1572.tbin
1573 112 NIH Molecular Libraries Probe Production Network [MLPCN] NIH Chemical Genomics Center [NCGC] MLPCN Grant: DK058080 Assay Provider: R. Kip Guy, St. Jude Children's Research Hospital NCGC Assay Overview: Thyroid receptor (TR) regulates many homeostatic processes including basal metabolism, cardiovascular function, body weight, and lipid trafficking. TR modulators are potential therapeutics for obesity and hyperlipidemias but current thyroid analogs have undesirable side effects, particularly card... aid1573.table aid1573.tbin
1574 1 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Probe Production Centers Network (MLPCN) Grant Number: 1 R03 MH084086-01 Assay Provider: Dr. Jose Luis Milan, Sanford-Burnham Medical Research Institute, San Diego CA Mineralization of cartilage and bone occurs by a series of physicochemical and biochemical processes that together facilitate the deposition of... aid1574.table aid1574.tbin
1575 2 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Probe Production Centers Network (MLPCN) Grant Number: 1 R03 MH084844-01 Assay Provider: Dr. John C. Reed, Sanford-Burnham Medical Research Institute, San Diego, CA The modulation of immune response activity is one of the major goals in the development of novel therapeutics for auto-immune and inflammatory disea... aid1575.table aid1575.tbin
1576 278 This study was conducted by EPA researchers to evaluate the validity of the rat uterine cytosolic (RUC) estrogen receptor (ER) competitive binding assay for use in the Endocrine Disruption Screening Program (EDSP). The assay measures the ability of radiolabeled 17-beta-estradiol (3H-E2) to bind with RUC ER in the presence of increasing concentrations of a test chemical. The goal is to employ this in vitro assay as a 1st tier screening tool for assessing the potential of structurally diverse envir... aid1576.table aid1576.tbin
1577 2 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Screening Centers Network (MLSCN) Grant Proposal Number: MH077602-01 Assay Provider: Dr. Jose Luis Millan, Sanford-Burnham Medical Research Institute, San Diego, CA. Alkaline phosphatase (EC 3.1.3.1) (APs) catalyze the hydrolysis of phosphomonoesters, releasing phosphate and alcohol. APs are dimeric enzymes found ... aid1577.table aid1577.tbin
1578 289584 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Probe Production Centers Network (MLPCN) Grant Number: 1 R03 MH084844-01 Assay Provider: Dr. John C. Reed, Sanford-Burnham Medical Research Institute, San Diego CA The modulation of immune response activity is one of the major goals in the development of novel therapeutics for auto-immune and inflammatory diseas... aid1578.table aid1578.tbin
1579 1 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Probe Production Centers Network (MLPCN) Grant Number: 1 R03 MH084844-01 Assay Provider: Dr. John C. Reed, Sanford-Burnham Medical Research Institute, San Diego CA The modulation of immune response activity is one of the major goals in the development of novel therapeutics for auto-immune and inflammatory diseases... aid1579.table aid1579.tbin
1580 1040 Screen for compounds that inhibit mitochondrial permeability transition. Disease: Huntington's Disease, ALS, Stroke, AD. The mitochondrial permeability transition may contribute to cell death in multiple neurodegenerative conditions aid1580.table aid1580.tbin
1581 1040 Disease: Neurodegeneration Rationale: Par4 is an emerging pivotal player that is well established as a mediator of neuronal degeneration in diseases such as: Alzheimer's, Parkinson's, Huntington's, amyotrophic lateral sclerosis, stroke and epileptic seizures. Par4 has been identified as a binding protein of the atypical protein kinase Cs (aPKC). Atypical PKCs play a critical role in regulation of the transcription factor NF-micro B, whose activity is required for neuronal survival. Formation of ... aid1581.table aid1581.tbin
1582 1040 Disease: Huntington's Disease Rationale: Huntington's Disease is a late onset neurodegenerative disease caused by expression of expanded polyglutamine tracts. A number of experimental models indicate that inhibition of histone acetyltranferase activity by expanded polyglutamine may be a primary cause of polyglutamine toxicity. Furthermore, application of histone deacetylase (HDAC) inhibitors have been shown to reduce polyglutamine toxicity in experimental models. In order to discover novel HDAC ... aid1582.table aid1582.tbin
1583 1040 This Assay identifies inhibitors of polyglutamine-induced caspase-3 activation. Spinal and Bulbar Muscular Atrophy (SBMA) is an X-linked recessive neurodegenerative disorder due to an expansion of the CAG triplet repeat sequence within exon 1 of the androgen receptor gene. Evidence indicates that expression of the androgen receptor with an expanded polyglutamine stretch induces apoptosis in culture cells.The androgen receptor is cleaved by caspase-3 and it has been proposed that caspase cleavage... aid1583.table aid1583.tbin
1584 1040 Disease: Huntington's disease Rationale: Huntington's disease (HD) is caused by an expanded CAG repeat encoding at a tract of consecutive glutamines near the N-terminus of huntingtin. The hypothesis that a conformational property capable of promoting aggregation is the crucial element in triggering HD pathogenesis leads directly to the notion that compounds that affect this property could have therapeutic potential. At present, it is uncertain whether the initiator of pathogenesis involves an in... aid1584.table aid1584.tbin
1585 1040 Disease: Huntington's Disease (HD) Rationale: Expanded polyglutamine (polyGln) diseases like Huntington's disease (HD) are typified by the formation and accumulation of the polyGln disease-related protein into insoluble aggregates in the nucleus and/or in the cytoplasm of the affected neurons. Given the potential role of polyGln aggregates and polyGln aggregate extension in the pathogenesis of expanded CAG repeat diseases, we developed a 96-well screening assay for polyGln aggregation inhibitors... aid1585.table aid1585.tbin
1586 1040 Disease: Huntington's Disease Rationale: The goal has been to develop a yeast 2-hybrid interaction which depends on interactions between polyglutamine-containing proteins. For this purpose, a set of six plasmids was constructed, each of which expresses a fusion of the first exon of huntingtin with GFP. This unit is followed by either a lexA DNA binding domain or by an artificial activation domain. In the huntingtin sequences, there were either 0, 25 or 103 glutamine residues. Expression from the... aid1586.table aid1586.tbin
1587 1040 Disease: Amyotrophic Lateral Sclerosis (ALS) Rationale: Amyotrophic Lateral Sclerosis (ALS) is the most common motor neuron disease in adults. Selective killing of motor neurons initiates a progressive paralysis in mid-life and is generally fatal within 1-5 years of onset. Several hypotheses for mechanisms that provoke or contribute to motor neuron death in ALS have been put forward. Mishandling of L-glutamate, the primary excitatory neurotransmitter in the mammalian CNS, results in repetitive m... aid1587.table aid1587.tbin
1588 1040 Disease: Polyglutamine repeat diseases including Huntington's disease Rationale: There are eight known diseases caused by an expansion in the polyglutamine region of specific proteins. The diseases include Huntington's disease, Spino-cerebellar ataxias, and Spino-bulbar muscular atrophies. They are all autosomal-dominant, late-onset neurodegenerative diseases characterized by neuronal death and the formation of protein aggregates. The pathogenesis of the diseases correlates with the number of re... aid1588.table aid1588.tbin
1589 1040 Disease: Polyglutamine Rationale: Intracellular inclusions are a common pathological hallmark of polyglutamine diseases, and are believed to reflect conformational abnormalities of the protein. Some cell-based models, including the differentiated PC12 neuralcells used in our assays, show inclusion formation that correlates with cytotoxicity. Moreover, genes that block toxicity in our cell-based system also reduce inclusion formation. In addition, genetic suppressors of polyQ toxicity in a fly mo... aid1589.table aid1589.tbin
1590 1040 Disease: Huntington's disease Rationale: Neuronal cell death in HD is a major cause of disability and is believed to be largely a dominant cell autonomous effect of the mutant huntingtin protein. The toxic species may be an N-terminal fragment of huntingtin. We have produced a cell model involving toxicity caused by expression of the N-terminal portion of huntingtin with an expanded repeat. Signal Type: smaller is better Literature Reference: Following the protocol of Cytotoxicity Detection ki... aid1590.table aid1590.tbin
1591 1 Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, Lake Nona, FL) Network: NIH Molecular Libraries Probe Production Network (MLPCN) Grant Proposal Number: Assay Provider: Dr. Layton Smith, Sanford-Burnham Medic